Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32545 and AZOBR_RS32550 are separated by 143 nucleotides AZOBR_RS32550 and AZOBR_RS32555 overlap by 8 nucleotides AZOBR_RS32555 and AZOBR_RS32560 are separated by 114 nucleotides
AZOBR_RS32545: AZOBR_RS32545 - hypothetical protein, at 665,889 to 666,413
_RS32545
AZOBR_RS32550: AZOBR_RS32550 - hypothetical protein, at 666,557 to 666,787
_RS32550
AZOBR_RS32555: AZOBR_RS32555 - membrane protein, at 666,780 to 667,823
_RS32555
AZOBR_RS32560: AZOBR_RS32560 - LysR family transcriptional regulator, at 667,938 to 668,819
_RS32560
Position (kb)
666
667
668 Strain fitness (log2 ratio)
-2
-1
0
1 at 665.789 kb on + strand at 665.789 kb on + strand at 665.789 kb on + strand at 665.790 kb on - strand at 665.790 kb on - strand at 666.411 kb on + strand at 666.411 kb on + strand at 666.411 kb on + strand at 666.411 kb on + strand at 666.411 kb on + strand at 666.411 kb on + strand at 666.411 kb on + strand at 667.061 kb on + strand, within AZOBR_RS32555 at 667.062 kb on - strand, within AZOBR_RS32555 at 667.062 kb on - strand, within AZOBR_RS32555 at 667.062 kb on - strand, within AZOBR_RS32555 at 667.239 kb on - strand, within AZOBR_RS32555 at 667.465 kb on + strand, within AZOBR_RS32555 at 667.466 kb on - strand, within AZOBR_RS32555 at 667.466 kb on - strand, within AZOBR_RS32555 at 667.884 kb on + strand at 667.887 kb on - strand at 667.906 kb on - strand at 668.413 kb on + strand, within AZOBR_RS32560 at 668.413 kb on + strand, within AZOBR_RS32560 at 668.413 kb on + strand, within AZOBR_RS32560 at 668.413 kb on + strand, within AZOBR_RS32560 at 668.413 kb on + strand, within AZOBR_RS32560 at 668.414 kb on - strand, within AZOBR_RS32560 at 668.414 kb on - strand, within AZOBR_RS32560 at 668.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 665,789 + -0.7 665,789 + -1.5 665,789 + -0.8 665,790 - +1.0 665,790 - +0.8 666,411 + -0.4 666,411 + +0.3 666,411 + +0.2 666,411 + +0.0 666,411 + -0.1 666,411 + -0.6 666,411 + +0.4 667,061 + AZOBR_RS32555 0.27 -0.2 667,062 - AZOBR_RS32555 0.27 -0.2 667,062 - AZOBR_RS32555 0.27 +0.5 667,062 - AZOBR_RS32555 0.27 -0.6 667,239 - AZOBR_RS32555 0.44 -1.3 667,465 + AZOBR_RS32555 0.66 -2.5 667,466 - AZOBR_RS32555 0.66 -0.1 667,466 - AZOBR_RS32555 0.66 -0.6 667,884 + +0.1 667,887 - +0.7 667,906 - -0.7 668,413 + AZOBR_RS32560 0.54 +0.0 668,413 + AZOBR_RS32560 0.54 +0.0 668,413 + AZOBR_RS32560 0.54 +0.5 668,413 + AZOBR_RS32560 0.54 -1.0 668,413 + AZOBR_RS32560 0.54 +0.2 668,414 - AZOBR_RS32560 0.54 +0.2 668,414 - AZOBR_RS32560 0.54 +0.5 668,817 + +0.8
Or see this region's nucleotide sequence