Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05960 and AZOBR_RS05965 are separated by 263 nucleotides AZOBR_RS05965 and AZOBR_RS05970 overlap by 4 nucleotides AZOBR_RS05970 and AZOBR_RS05975 are separated by 67 nucleotides AZOBR_RS05975 and AZOBR_RS05980 are separated by 107 nucleotides
AZOBR_RS05960: AZOBR_RS05960 - hypothetical protein, at 1,265,537 to 1,265,941
_RS05960
AZOBR_RS05965: AZOBR_RS05965 - hypothetical protein, at 1,266,205 to 1,266,576
_RS05965
AZOBR_RS05970: AZOBR_RS05970 - 4-carboxymuconolactone decarboxylase, at 1,266,573 to 1,267,040
_RS05970
AZOBR_RS05975: AZOBR_RS05975 - oxidoreductase, at 1,267,108 to 1,267,839
_RS05975
AZOBR_RS05980: AZOBR_RS05980 - LysR family transcriptional regulator, at 1,267,947 to 1,268,867
_RS05980
Position (kb)
1266
1267
1268 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1265.625 kb on + strand, within AZOBR_RS05960 at 1265.625 kb on + strand, within AZOBR_RS05960 at 1265.625 kb on + strand, within AZOBR_RS05960 at 1265.625 kb on + strand, within AZOBR_RS05960 at 1265.709 kb on + strand, within AZOBR_RS05960 at 1265.790 kb on + strand, within AZOBR_RS05960 at 1265.818 kb on - strand, within AZOBR_RS05960 at 1265.818 kb on - strand, within AZOBR_RS05960 at 1265.836 kb on - strand, within AZOBR_RS05960 at 1265.838 kb on + strand, within AZOBR_RS05960 at 1265.872 kb on - strand, within AZOBR_RS05960 at 1265.946 kb on + strand at 1265.958 kb on + strand at 1265.958 kb on + strand at 1265.976 kb on + strand at 1266.015 kb on + strand at 1266.016 kb on - strand at 1266.332 kb on + strand, within AZOBR_RS05965 at 1266.332 kb on + strand, within AZOBR_RS05965 at 1266.332 kb on + strand, within AZOBR_RS05965 at 1266.333 kb on - strand, within AZOBR_RS05965 at 1266.365 kb on + strand, within AZOBR_RS05965 at 1266.365 kb on + strand, within AZOBR_RS05965 at 1266.429 kb on - strand, within AZOBR_RS05965 at 1266.429 kb on - strand, within AZOBR_RS05965 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.676 kb on + strand, within AZOBR_RS05970 at 1266.677 kb on - strand, within AZOBR_RS05970 at 1266.677 kb on - strand, within AZOBR_RS05970 at 1266.677 kb on - strand, within AZOBR_RS05970 at 1266.841 kb on + strand, within AZOBR_RS05970 at 1266.841 kb on + strand, within AZOBR_RS05970 at 1266.842 kb on - strand, within AZOBR_RS05970 at 1267.224 kb on - strand, within AZOBR_RS05975 at 1267.361 kb on + strand, within AZOBR_RS05975 at 1267.362 kb on - strand, within AZOBR_RS05975 at 1267.376 kb on + strand, within AZOBR_RS05975 at 1267.376 kb on + strand, within AZOBR_RS05975 at 1267.376 kb on + strand, within AZOBR_RS05975 at 1267.481 kb on + strand, within AZOBR_RS05975 at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.883 kb on + strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand at 1267.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,265,625 + AZOBR_RS05960 0.22 +0.0 1,265,625 + AZOBR_RS05960 0.22 -0.2 1,265,625 + AZOBR_RS05960 0.22 +0.3 1,265,625 + AZOBR_RS05960 0.22 -1.4 1,265,709 + AZOBR_RS05960 0.42 -0.2 1,265,790 + AZOBR_RS05960 0.62 +0.4 1,265,818 - AZOBR_RS05960 0.69 -0.3 1,265,818 - AZOBR_RS05960 0.69 +0.6 1,265,836 - AZOBR_RS05960 0.74 -0.1 1,265,838 + AZOBR_RS05960 0.74 -2.3 1,265,872 - AZOBR_RS05960 0.83 -0.6 1,265,946 + +0.4 1,265,958 + +0.2 1,265,958 + -1.6 1,265,976 + +0.1 1,266,015 + +0.1 1,266,016 - -0.4 1,266,332 + AZOBR_RS05965 0.34 +0.3 1,266,332 + AZOBR_RS05965 0.34 +0.3 1,266,332 + AZOBR_RS05965 0.34 -0.2 1,266,333 - AZOBR_RS05965 0.34 -0.5 1,266,365 + AZOBR_RS05965 0.43 +0.3 1,266,365 + AZOBR_RS05965 0.43 +0.3 1,266,429 - AZOBR_RS05965 0.60 +0.3 1,266,429 - AZOBR_RS05965 0.60 +0.1 1,266,676 + AZOBR_RS05970 0.22 -1.5 1,266,676 + AZOBR_RS05970 0.22 -0.5 1,266,676 + AZOBR_RS05970 0.22 -0.3 1,266,676 + AZOBR_RS05970 0.22 -0.8 1,266,676 + AZOBR_RS05970 0.22 -1.5 1,266,676 + AZOBR_RS05970 0.22 +0.8 1,266,677 - AZOBR_RS05970 0.22 -0.4 1,266,677 - AZOBR_RS05970 0.22 -0.2 1,266,677 - AZOBR_RS05970 0.22 -0.0 1,266,841 + AZOBR_RS05970 0.57 -0.9 1,266,841 + AZOBR_RS05970 0.57 -0.5 1,266,842 - AZOBR_RS05970 0.57 -0.1 1,267,224 - AZOBR_RS05975 0.16 +0.1 1,267,361 + AZOBR_RS05975 0.35 +0.1 1,267,362 - AZOBR_RS05975 0.35 +1.4 1,267,376 + AZOBR_RS05975 0.37 -0.0 1,267,376 + AZOBR_RS05975 0.37 +0.5 1,267,376 + AZOBR_RS05975 0.37 +1.4 1,267,481 + AZOBR_RS05975 0.51 -0.2 1,267,883 + +0.3 1,267,883 + -0.3 1,267,883 + +0.5 1,267,883 + -0.3 1,267,883 + +0.2 1,267,883 + -0.5 1,267,883 + +0.8 1,267,883 + -0.5 1,267,884 - +0.7 1,267,884 - -0.4 1,267,884 - -0.3 1,267,884 - +0.1 1,267,884 - +0.8 1,267,884 - -0.2 1,267,884 - +1.8 1,267,884 - +0.2
Or see this region's nucleotide sequence