Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00640 and AZOBR_RS00645 are separated by 20 nucleotides AZOBR_RS00645 and AZOBR_RS00650 overlap by 4 nucleotides AZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotides
AZOBR_RS00640: AZOBR_RS00640 - pyrophosphorylase, at 128,739 to 129,566
_RS00640
AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576
_RS00645
AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481
_RS00650
AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833
_RS00655
Position (kb) 129
130
131 Strain fitness (log2 ratio) -1
0
1
2 at 128.648 kb on - strand at 128.648 kb on - strand at 128.650 kb on - strand at 128.828 kb on - strand, within AZOBR_RS00640 at 129.588 kb on + strand at 129.588 kb on + strand at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.378 kb on - strand, within AZOBR_RS00645
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 128,648 - +0.9 128,648 - -0.8 128,650 - +1.0 128,828 - AZOBR_RS00640 0.11 -1.4 129,588 + +1.8 129,588 + -1.1 130,356 + AZOBR_RS00645 0.78 +0.0 130,356 + AZOBR_RS00645 0.78 +0.3 130,356 + AZOBR_RS00645 0.78 -0.6 130,357 - AZOBR_RS00645 0.78 +1.2 130,357 - AZOBR_RS00645 0.78 +0.3 130,378 - AZOBR_RS00645 0.80 +1.8
Or see this region's nucleotide sequence