Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08720

Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08715 and MMP_RS08720 are separated by 29 nucleotidesMMP_RS08720 and MMP_RS09070 are separated by 89 nucleotidesMMP_RS09070 and MMP_RS08725 are separated by 15 nucleotides MMP_RS08715: MMP_RS08715 - formylmethanofuran dehydrogenase subunit B, at 1,631,371 to 1,632,675 _RS08715 MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892 _RS08720 MMP_RS09070: MMP_RS09070 - F420 non-reducing hydrogenase subunit, at 1,633,982 to 1,634,116 _RS09070 MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388 _RS08725 Position (kb) 1632 1633 1634Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1631.827 kb on + strand, within MMP_RS08715at 1631.829 kb on - strand, within MMP_RS08715at 1632.471 kb on + strand, within MMP_RS08715at 1632.652 kb on - strandat 1632.782 kb on - strandat 1633.411 kb on + strand, within MMP_RS08720at 1633.412 kb on - strand, within MMP_RS08720at 1633.668 kb on - strand, within MMP_RS08720at 1633.746 kb on - strand, within MMP_RS08720at 1634.354 kb on - strand, within MMP_RS08725at 1634.721 kb on - strand, within MMP_RS08725

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations
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1,631,827 + MMP_RS08715 0.35 -3.6
1,631,829 - MMP_RS08715 0.35 -3.5
1,632,471 + MMP_RS08715 0.84 -4.5
1,632,652 - -3.1
1,632,782 - -3.3
1,633,411 + MMP_RS08720 0.59 -3.1
1,633,412 - MMP_RS08720 0.60 -3.1
1,633,668 - MMP_RS08720 0.81 -2.2
1,633,746 - MMP_RS08720 0.88 -4.2
1,634,354 - MMP_RS08725 0.18 +0.5
1,634,721 - MMP_RS08725 0.47 -3.6

Or see this region's nucleotide sequence