Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07985 and MMP_RS07990 are separated by 160 nucleotides MMP_RS07990 and MMP_RS07995 are separated by 129 nucleotides MMP_RS07995 and MMP_RS08000 are separated by 72 nucleotides MMP_RS08000 and MMP_RS08005 are separated by 259 nucleotides
MMP_RS07985: MMP_RS07985 - nitroreductase family protein, at 1,506,963 to 1,507,565
_RS07985
MMP_RS07990: MMP_RS07990 - tRNA-Leu, at 1,507,726 to 1,507,810
_RS07990
MMP_RS07995: MMP_RS07995 - tRNA-Met, at 1,507,940 to 1,508,054
_RS07995
MMP_RS08000: MMP_RS08000 - methyl coenzyme M reductase-arginine methyltransferase Mmp10, at 1,508,127 to 1,509,437
_RS08000
MMP_RS08005: MMP_RS08005 - coenzyme-B sulfoethylthiotransferase subunit beta, at 1,509,697 to 1,511,028
_RS08005
Position (kb)
1508
1509
1510 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1507.167 kb on - strand, within MMP_RS07985 at 1507.186 kb on + strand, within MMP_RS07985 at 1507.243 kb on - strand, within MMP_RS07985 at 1507.247 kb on - strand, within MMP_RS07985 at 1507.266 kb on + strand, within MMP_RS07985 at 1507.266 kb on + strand, within MMP_RS07985 at 1507.329 kb on - strand, within MMP_RS07985 at 1507.329 kb on - strand, within MMP_RS07985 at 1507.332 kb on - strand, within MMP_RS07985 at 1507.377 kb on - strand, within MMP_RS07985 at 1507.377 kb on - strand, within MMP_RS07985 at 1507.379 kb on - strand, within MMP_RS07985 at 1507.379 kb on - strand, within MMP_RS07985 at 1507.379 kb on - strand, within MMP_RS07985 at 1507.379 kb on - strand, within MMP_RS07985 at 1507.379 kb on - strand, within MMP_RS07985 at 1507.380 kb on + strand, within MMP_RS07985 at 1507.380 kb on + strand, within MMP_RS07985 at 1507.380 kb on + strand, within MMP_RS07985 at 1507.382 kb on + strand, within MMP_RS07985 at 1507.382 kb on + strand, within MMP_RS07985 at 1507.382 kb on + strand, within MMP_RS07985 at 1507.470 kb on - strand, within MMP_RS07985 at 1507.470 kb on - strand, within MMP_RS07985 at 1507.503 kb on - strand, within MMP_RS07985 at 1507.504 kb on + strand, within MMP_RS07985 at 1507.519 kb on - strand at 1507.520 kb on + strand at 1507.542 kb on - strand at 1507.543 kb on + strand at 1507.543 kb on + strand at 1507.585 kb on - strand at 1507.588 kb on - strand at 1507.590 kb on - strand at 1507.591 kb on + strand at 1507.591 kb on + strand at 1507.615 kb on + strand at 1507.616 kb on - strand at 1507.616 kb on - strand at 1507.617 kb on + strand at 1508.112 kb on + strand at 1508.294 kb on - strand, within MMP_RS08000 at 1508.341 kb on + strand, within MMP_RS08000 at 1508.352 kb on - strand, within MMP_RS08000 at 1508.361 kb on - strand, within MMP_RS08000 at 1508.391 kb on + strand, within MMP_RS08000 at 1508.461 kb on - strand, within MMP_RS08000 at 1508.462 kb on + strand, within MMP_RS08000 at 1508.463 kb on - strand, within MMP_RS08000 at 1508.464 kb on + strand, within MMP_RS08000 at 1508.465 kb on - strand, within MMP_RS08000 at 1508.465 kb on - strand, within MMP_RS08000 at 1508.465 kb on - strand, within MMP_RS08000 at 1508.465 kb on - strand, within MMP_RS08000 at 1508.465 kb on - strand, within MMP_RS08000 at 1508.466 kb on + strand, within MMP_RS08000 at 1508.466 kb on + strand, within MMP_RS08000 at 1508.466 kb on + strand, within MMP_RS08000 at 1508.466 kb on + strand, within MMP_RS08000 at 1508.468 kb on + strand, within MMP_RS08000 at 1508.487 kb on - strand, within MMP_RS08000 at 1508.546 kb on + strand, within MMP_RS08000 at 1508.671 kb on + strand, within MMP_RS08000 at 1508.702 kb on - strand, within MMP_RS08000 at 1508.720 kb on - strand, within MMP_RS08000 at 1508.790 kb on - strand, within MMP_RS08000 at 1508.791 kb on + strand, within MMP_RS08000 at 1508.974 kb on - strand, within MMP_RS08000 at 1509.014 kb on - strand, within MMP_RS08000 at 1509.014 kb on - strand, within MMP_RS08000 at 1509.015 kb on + strand, within MMP_RS08000 at 1509.016 kb on - strand, within MMP_RS08000 at 1509.021 kb on - strand, within MMP_RS08000 at 1509.021 kb on - strand, within MMP_RS08000 at 1509.237 kb on + strand, within MMP_RS08000 at 1509.438 kb on + strand at 1509.449 kb on + strand at 1509.452 kb on - strand at 1509.453 kb on + strand at 1509.467 kb on + strand at 1509.502 kb on - strand at 1509.533 kb on - strand at 1509.549 kb on + strand at 1509.604 kb on + strand at 1509.604 kb on + strand at 1509.642 kb on - strand at 1509.642 kb on - strand at 1509.643 kb on + strand at 1509.668 kb on - strand at 1509.670 kb on - strand at 1509.696 kb on - strand at 1509.862 kb on + strand at 1510.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations remove 1,507,167 - MMP_RS07985 0.34 +0.5 1,507,186 + MMP_RS07985 0.37 +0.3 1,507,243 - MMP_RS07985 0.46 +0.9 1,507,247 - MMP_RS07985 0.47 -0.7 1,507,266 + MMP_RS07985 0.50 -0.0 1,507,266 + MMP_RS07985 0.50 +0.7 1,507,329 - MMP_RS07985 0.61 +0.6 1,507,329 - MMP_RS07985 0.61 +0.5 1,507,332 - MMP_RS07985 0.61 +0.5 1,507,377 - MMP_RS07985 0.69 +1.0 1,507,377 - MMP_RS07985 0.69 +0.6 1,507,379 - MMP_RS07985 0.69 +0.5 1,507,379 - MMP_RS07985 0.69 +1.1 1,507,379 - MMP_RS07985 0.69 +0.7 1,507,379 - MMP_RS07985 0.69 +1.2 1,507,379 - MMP_RS07985 0.69 +0.8 1,507,380 + MMP_RS07985 0.69 +0.3 1,507,380 + MMP_RS07985 0.69 +0.6 1,507,380 + MMP_RS07985 0.69 +0.8 1,507,382 + MMP_RS07985 0.69 +0.7 1,507,382 + MMP_RS07985 0.69 +0.0 1,507,382 + MMP_RS07985 0.69 +0.4 1,507,470 - MMP_RS07985 0.84 +0.4 1,507,470 - MMP_RS07985 0.84 +0.6 1,507,503 - MMP_RS07985 0.90 +0.7 1,507,504 + MMP_RS07985 0.90 +0.6 1,507,519 - +0.4 1,507,520 + +0.4 1,507,542 - +0.8 1,507,543 + +0.7 1,507,543 + +1.1 1,507,585 - +0.3 1,507,588 - +1.1 1,507,590 - +0.6 1,507,591 + +0.9 1,507,591 + +0.2 1,507,615 + +0.7 1,507,616 - +1.3 1,507,616 - +0.1 1,507,617 + +0.9 1,508,112 + +0.3 1,508,294 - MMP_RS08000 0.13 -2.6 1,508,341 + MMP_RS08000 0.16 -3.7 1,508,352 - MMP_RS08000 0.17 -4.0 1,508,361 - MMP_RS08000 0.18 -3.5 1,508,391 + MMP_RS08000 0.20 -3.9 1,508,461 - MMP_RS08000 0.25 -6.2 1,508,462 + MMP_RS08000 0.26 -4.4 1,508,463 - MMP_RS08000 0.26 -3.2 1,508,464 + MMP_RS08000 0.26 -4.0 1,508,465 - MMP_RS08000 0.26 -3.9 1,508,465 - MMP_RS08000 0.26 -4.0 1,508,465 - MMP_RS08000 0.26 -4.2 1,508,465 - MMP_RS08000 0.26 -3.6 1,508,465 - MMP_RS08000 0.26 -3.7 1,508,466 + MMP_RS08000 0.26 -3.4 1,508,466 + MMP_RS08000 0.26 -3.7 1,508,466 + MMP_RS08000 0.26 -3.7 1,508,466 + MMP_RS08000 0.26 -3.0 1,508,468 + MMP_RS08000 0.26 -3.3 1,508,487 - MMP_RS08000 0.27 -4.2 1,508,546 + MMP_RS08000 0.32 -3.0 1,508,671 + MMP_RS08000 0.41 -4.1 1,508,702 - MMP_RS08000 0.44 -3.2 1,508,720 - MMP_RS08000 0.45 -5.2 1,508,790 - MMP_RS08000 0.51 -3.2 1,508,791 + MMP_RS08000 0.51 -4.4 1,508,974 - MMP_RS08000 0.65 -3.1 1,509,014 - MMP_RS08000 0.68 -4.7 1,509,014 - MMP_RS08000 0.68 -2.1 1,509,015 + MMP_RS08000 0.68 -4.8 1,509,016 - MMP_RS08000 0.68 -3.6 1,509,021 - MMP_RS08000 0.68 -3.8 1,509,021 - MMP_RS08000 0.68 -4.0 1,509,237 + MMP_RS08000 0.85 -4.0 1,509,438 + -3.1 1,509,449 + -3.0 1,509,452 - -3.1 1,509,453 + -4.2 1,509,467 + -5.0 1,509,502 - -3.5 1,509,533 - -2.0 1,509,549 + -4.9 1,509,604 + -3.4 1,509,604 + -3.2 1,509,642 - -3.4 1,509,642 - -3.7 1,509,643 + -3.6 1,509,668 - -3.4 1,509,670 - -1.4 1,509,696 - -3.5 1,509,862 + -3.3 1,510,260 + -3.0
Or see this region's nucleotide sequence