Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06985 and MMP_RS06990 are separated by 15 nucleotides MMP_RS06990 and MMP_RS06995 are separated by 17 nucleotides MMP_RS06995 and MMP_RS07000 are separated by 15 nucleotides MMP_RS07000 and MMP_RS07005 are separated by 44 nucleotides MMP_RS07005 and MMP_RS07010 are separated by 13 nucleotides
MMP_RS06985: MMP_RS06985 - tRNA 4-thiouridine(8) synthase ThiI, at 1,333,713 to 1,334,864
_RS06985
MMP_RS06990: MMP_RS06990 - CatB-related O-acetyltransferase, at 1,334,880 to 1,335,560
_RS06990
MMP_RS06995: MMP_RS06995 - TIGR00269 family protein, at 1,335,578 to 1,336,513
_RS06995
MMP_RS07000: MMP_RS07000 - MoaD/ThiS family protein, at 1,336,529 to 1,336,783
_RS07000
MMP_RS07005: MMP_RS07005 - 4Fe-4S binding protein, at 1,336,828 to 1,337,226
_RS07005
MMP_RS07010: MMP_RS07010 - homocysteine biosynthesis protein, at 1,337,240 to 1,338,781
_RS07010
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1334.750 kb on + strand at 1334.844 kb on + strand at 1334.868 kb on + strand at 1334.877 kb on + strand at 1335.025 kb on + strand, within MMP_RS06990 at 1335.053 kb on - strand, within MMP_RS06990 at 1335.061 kb on + strand, within MMP_RS06990 at 1335.095 kb on - strand, within MMP_RS06990 at 1335.104 kb on - strand, within MMP_RS06990 at 1335.144 kb on + strand, within MMP_RS06990 at 1335.271 kb on - strand, within MMP_RS06990 at 1335.286 kb on - strand, within MMP_RS06990 at 1335.287 kb on + strand, within MMP_RS06990 at 1335.360 kb on + strand, within MMP_RS06990 at 1335.377 kb on + strand, within MMP_RS06990 at 1335.503 kb on - strand at 1335.504 kb on + strand at 1335.510 kb on + strand at 1335.745 kb on + strand at 1336.447 kb on - strand at 1336.448 kb on + strand at 1336.480 kb on + strand at 1336.481 kb on - strand at 1336.517 kb on + strand at 1336.551 kb on - strand at 1336.553 kb on - strand at 1336.553 kb on - strand at 1336.580 kb on - strand at 1336.581 kb on + strand at 1336.622 kb on - strand at 1336.685 kb on - strand at 1336.827 kb on + strand at 1337.128 kb on - strand, within MMP_RS07005 at 1337.405 kb on - strand at 1337.406 kb on + strand at 1337.407 kb on - strand at 1337.407 kb on - strand at 1337.407 kb on - strand at 1337.408 kb on + strand at 1337.409 kb on - strand at 1337.409 kb on - strand at 1337.410 kb on + strand at 1337.410 kb on + strand at 1337.414 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations remove 1,334,750 + -2.8 1,334,844 + +0.5 1,334,868 + -0.2 1,334,877 + +0.1 1,335,025 + MMP_RS06990 0.21 +0.2 1,335,053 - MMP_RS06990 0.25 -0.1 1,335,061 + MMP_RS06990 0.27 +0.1 1,335,095 - MMP_RS06990 0.32 -0.2 1,335,104 - MMP_RS06990 0.33 +0.3 1,335,144 + MMP_RS06990 0.39 -0.0 1,335,271 - MMP_RS06990 0.57 +0.1 1,335,286 - MMP_RS06990 0.60 +0.3 1,335,287 + MMP_RS06990 0.60 -1.3 1,335,360 + MMP_RS06990 0.70 +0.6 1,335,377 + MMP_RS06990 0.73 +0.6 1,335,503 - -0.3 1,335,504 + +0.7 1,335,510 + -0.2 1,335,745 + -4.2 1,336,447 - -3.3 1,336,448 + -3.2 1,336,480 + -3.7 1,336,481 - -4.7 1,336,517 + -4.2 1,336,551 - -5.3 1,336,553 - -7.4 1,336,553 - -4.2 1,336,580 - -6.4 1,336,581 + -4.4 1,336,622 - -5.9 1,336,685 - -5.0 1,336,827 + -6.3 1,337,128 - MMP_RS07005 0.75 -4.3 1,337,405 - -3.9 1,337,406 + -3.4 1,337,407 - -4.2 1,337,407 - -3.7 1,337,407 - -4.4 1,337,408 + -3.8 1,337,409 - -2.0 1,337,409 - -3.3 1,337,410 + -1.8 1,337,410 + -3.1 1,337,414 + -4.7
Or see this region's nucleotide sequence