Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17635 and EX31_RS25530 are separated by 98 nucleotides EX31_RS25530 and EX31_RS17640 are separated by 21 nucleotides EX31_RS17640 and EX31_RS17645 are separated by 33 nucleotides EX31_RS17645 and EX31_RS17650 are separated by 97 nucleotides
EX31_RS17635: EX31_RS17635 - winged helix-turn-helix transcriptional regulator, at 78,512 to 78,994
_RS17635
EX31_RS25530: EX31_RS25530 - hypothetical protein, at 79,093 to 79,212
_RS25530
EX31_RS17640: EX31_RS17640 - TetR/AcrR family transcriptional regulator, at 79,234 to 79,470
_RS17640
EX31_RS17645: EX31_RS17645 - SDR family oxidoreductase, at 79,504 to 80,559
_RS17645
EX31_RS17650: EX31_RS17650 - LysR family transcriptional regulator, at 80,657 to 81,553
_RS17650
Position (kb) 79
80
81 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 78.535 kb on + strand at 78.535 kb on + strand at 78.535 kb on + strand at 78.558 kb on + strand at 78.565 kb on + strand, within EX31_RS17635 at 78.565 kb on + strand, within EX31_RS17635 at 78.565 kb on + strand, within EX31_RS17635 at 78.565 kb on + strand, within EX31_RS17635 at 78.566 kb on - strand, within EX31_RS17635 at 78.637 kb on + strand, within EX31_RS17635 at 78.637 kb on + strand, within EX31_RS17635 at 78.691 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.693 kb on + strand, within EX31_RS17635 at 78.694 kb on - strand, within EX31_RS17635 at 78.694 kb on - strand, within EX31_RS17635 at 78.699 kb on + strand, within EX31_RS17635 at 78.700 kb on - strand, within EX31_RS17635 at 78.733 kb on + strand, within EX31_RS17635 at 78.733 kb on + strand, within EX31_RS17635 at 78.734 kb on - strand, within EX31_RS17635 at 78.753 kb on + strand, within EX31_RS17635 at 78.757 kb on + strand, within EX31_RS17635 at 78.757 kb on + strand, within EX31_RS17635 at 78.757 kb on + strand, within EX31_RS17635 at 78.884 kb on + strand, within EX31_RS17635 at 78.884 kb on + strand, within EX31_RS17635 at 78.887 kb on - strand, within EX31_RS17635 at 78.901 kb on + strand, within EX31_RS17635 at 79.003 kb on + strand at 79.003 kb on + strand at 79.003 kb on + strand at 79.003 kb on + strand at 79.004 kb on - strand at 79.004 kb on - strand at 79.004 kb on - strand at 79.004 kb on - strand at 79.004 kb on - strand at 79.009 kb on + strand at 79.009 kb on + strand at 79.010 kb on - strand at 79.035 kb on + strand at 79.036 kb on - strand at 79.046 kb on + strand at 79.047 kb on - strand at 79.056 kb on + strand at 79.056 kb on + strand at 79.079 kb on - strand at 79.079 kb on - strand at 79.079 kb on - strand at 79.116 kb on - strand, within EX31_RS25530 at 79.125 kb on + strand, within EX31_RS25530 at 79.125 kb on + strand, within EX31_RS25530 at 79.126 kb on - strand, within EX31_RS25530 at 79.126 kb on - strand, within EX31_RS25530 at 79.126 kb on - strand, within EX31_RS25530 at 79.126 kb on - strand, within EX31_RS25530 at 79.212 kb on - strand at 79.232 kb on + strand at 79.233 kb on - strand at 79.350 kb on - strand, within EX31_RS17640 at 79.404 kb on - strand, within EX31_RS17640 at 79.404 kb on - strand, within EX31_RS17640 at 79.404 kb on - strand, within EX31_RS17640 at 79.446 kb on - strand, within EX31_RS17640 at 79.476 kb on - strand at 79.476 kb on - strand at 79.485 kb on + strand at 79.485 kb on + strand at 79.486 kb on - strand at 79.486 kb on - strand at 79.486 kb on - strand at 79.579 kb on - strand at 79.622 kb on + strand, within EX31_RS17645 at 79.623 kb on - strand, within EX31_RS17645 at 79.724 kb on + strand, within EX31_RS17645 at 79.724 kb on + strand, within EX31_RS17645 at 79.725 kb on - strand, within EX31_RS17645 at 79.725 kb on - strand, within EX31_RS17645 at 79.725 kb on - strand, within EX31_RS17645 at 79.930 kb on - strand, within EX31_RS17645 at 79.930 kb on - strand, within EX31_RS17645 at 79.930 kb on - strand, within EX31_RS17645 at 79.930 kb on - strand, within EX31_RS17645 at 79.930 kb on - strand, within EX31_RS17645 at 79.966 kb on + strand, within EX31_RS17645 at 79.980 kb on + strand, within EX31_RS17645 at 79.995 kb on - strand, within EX31_RS17645 at 80.199 kb on - strand, within EX31_RS17645 at 80.207 kb on + strand, within EX31_RS17645 at 80.224 kb on + strand, within EX31_RS17645 at 80.224 kb on + strand, within EX31_RS17645 at 80.225 kb on - strand, within EX31_RS17645 at 80.407 kb on + strand, within EX31_RS17645 at 80.418 kb on + strand, within EX31_RS17645 at 80.418 kb on + strand, within EX31_RS17645 at 80.419 kb on - strand, within EX31_RS17645 at 80.419 kb on - strand, within EX31_RS17645 at 80.419 kb on - strand, within EX31_RS17645 at 80.435 kb on - strand, within EX31_RS17645 at 80.435 kb on - strand, within EX31_RS17645 at 80.435 kb on - strand, within EX31_RS17645 at 80.553 kb on + strand at 80.553 kb on + strand at 80.554 kb on - strand at 80.554 kb on - strand at 80.554 kb on - strand at 80.554 kb on - strand at 80.554 kb on - strand at 80.586 kb on + strand at 80.586 kb on + strand at 80.586 kb on + strand at 80.586 kb on + strand at 80.586 kb on + strand at 80.586 kb on + strand at 80.587 kb on - strand at 80.596 kb on + strand at 80.596 kb on + strand at 80.596 kb on + strand at 80.599 kb on - strand at 80.599 kb on - strand at 80.616 kb on + strand at 80.616 kb on + strand at 80.617 kb on - strand at 80.617 kb on - strand at 80.639 kb on + strand at 80.640 kb on - strand at 80.665 kb on + strand at 80.665 kb on + strand at 80.665 kb on + strand at 80.666 kb on - strand at 80.799 kb on + strand, within EX31_RS17650 at 80.800 kb on - strand, within EX31_RS17650 at 80.812 kb on + strand, within EX31_RS17650 at 80.812 kb on + strand, within EX31_RS17650 at 80.813 kb on - strand, within EX31_RS17650 at 80.813 kb on - strand, within EX31_RS17650 at 80.813 kb on - strand, within EX31_RS17650 at 80.980 kb on - strand, within EX31_RS17650 at 81.025 kb on + strand, within EX31_RS17650 at 81.136 kb on + strand, within EX31_RS17650 at 81.169 kb on + strand, within EX31_RS17650 at 81.246 kb on + strand, within EX31_RS17650 at 81.271 kb on + strand, within EX31_RS17650 at 81.286 kb on + strand, within EX31_RS17650 at 81.286 kb on + strand, within EX31_RS17650 at 81.286 kb on + strand, within EX31_RS17650 at 81.287 kb on - strand, within EX31_RS17650 at 81.287 kb on - strand, within EX31_RS17650 at 81.289 kb on + strand, within EX31_RS17650 at 81.290 kb on - strand, within EX31_RS17650 at 81.307 kb on + strand, within EX31_RS17650 at 81.324 kb on + strand, within EX31_RS17650 at 81.324 kb on + strand, within EX31_RS17650 at 81.324 kb on + strand, within EX31_RS17650 at 81.367 kb on - strand, within EX31_RS17650 at 81.367 kb on - strand, within EX31_RS17650 at 81.456 kb on - strand, within EX31_RS17650 at 81.473 kb on - strand at 81.504 kb on + strand at 81.505 kb on - strand at 81.549 kb on + strand at 81.549 kb on + strand at 81.549 kb on + strand at 81.550 kb on - strand at 81.550 kb on - strand at 81.550 kb on - strand at 81.550 kb on - strand at 81.550 kb on - strand at 81.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 78,535 + +0.1 78,535 + +0.5 78,535 + -1.0 78,558 + -0.2 78,565 + EX31_RS17635 0.11 -0.3 78,565 + EX31_RS17635 0.11 +1.9 78,565 + EX31_RS17635 0.11 +1.1 78,565 + EX31_RS17635 0.11 +0.6 78,566 - EX31_RS17635 0.11 +1.5 78,637 + EX31_RS17635 0.26 +0.3 78,637 + EX31_RS17635 0.26 +0.2 78,691 + EX31_RS17635 0.37 +1.1 78,693 + EX31_RS17635 0.37 -0.4 78,693 + EX31_RS17635 0.37 +0.8 78,693 + EX31_RS17635 0.37 +0.5 78,693 + EX31_RS17635 0.37 +0.7 78,693 + EX31_RS17635 0.37 +0.1 78,693 + EX31_RS17635 0.37 -0.1 78,693 + EX31_RS17635 0.37 +0.5 78,693 + EX31_RS17635 0.37 -0.5 78,693 + EX31_RS17635 0.37 -0.5 78,694 - EX31_RS17635 0.38 -0.0 78,694 - EX31_RS17635 0.38 -0.1 78,699 + EX31_RS17635 0.39 -0.8 78,700 - EX31_RS17635 0.39 -0.1 78,733 + EX31_RS17635 0.46 +1.2 78,733 + EX31_RS17635 0.46 -1.2 78,734 - EX31_RS17635 0.46 +0.8 78,753 + EX31_RS17635 0.50 +1.1 78,757 + EX31_RS17635 0.51 +0.5 78,757 + EX31_RS17635 0.51 -0.1 78,757 + EX31_RS17635 0.51 +0.5 78,884 + EX31_RS17635 0.77 -0.3 78,884 + EX31_RS17635 0.77 -0.7 78,887 - EX31_RS17635 0.78 +1.1 78,901 + EX31_RS17635 0.81 -0.5 79,003 + -0.5 79,003 + +0.9 79,003 + +1.3 79,003 + +0.1 79,004 - +0.3 79,004 - -0.7 79,004 - +0.1 79,004 - +0.4 79,004 - +1.9 79,009 + -0.5 79,009 + -0.2 79,010 - +1.0 79,035 + +1.1 79,036 - +0.9 79,046 + -2.0 79,047 - -0.1 79,056 + +0.7 79,056 + -0.1 79,079 - -0.6 79,079 - -0.6 79,079 - +0.8 79,116 - EX31_RS25530 0.19 -1.5 79,125 + EX31_RS25530 0.27 +1.9 79,125 + EX31_RS25530 0.27 +0.6 79,126 - EX31_RS25530 0.28 +0.3 79,126 - EX31_RS25530 0.28 +0.7 79,126 - EX31_RS25530 0.28 -0.5 79,126 - EX31_RS25530 0.28 -0.6 79,212 - -0.4 79,232 + -0.8 79,233 - -0.1 79,350 - EX31_RS17640 0.49 -0.1 79,404 - EX31_RS17640 0.72 +0.6 79,404 - EX31_RS17640 0.72 +1.9 79,404 - EX31_RS17640 0.72 +0.5 79,446 - EX31_RS17640 0.89 +1.1 79,476 - -0.5 79,476 - +1.3 79,485 + +1.9 79,485 + -0.0 79,486 - -0.1 79,486 - -0.1 79,486 - +1.2 79,579 - +0.4 79,622 + EX31_RS17645 0.11 +1.5 79,623 - EX31_RS17645 0.11 +0.4 79,724 + EX31_RS17645 0.21 +0.1 79,724 + EX31_RS17645 0.21 -0.0 79,725 - EX31_RS17645 0.21 +0.3 79,725 - EX31_RS17645 0.21 -1.1 79,725 - EX31_RS17645 0.21 +0.3 79,930 - EX31_RS17645 0.40 -0.2 79,930 - EX31_RS17645 0.40 +0.3 79,930 - EX31_RS17645 0.40 -1.5 79,930 - EX31_RS17645 0.40 -0.1 79,930 - EX31_RS17645 0.40 +1.0 79,966 + EX31_RS17645 0.44 -0.0 79,980 + EX31_RS17645 0.45 +0.9 79,995 - EX31_RS17645 0.46 +0.5 80,199 - EX31_RS17645 0.66 -0.3 80,207 + EX31_RS17645 0.67 -0.1 80,224 + EX31_RS17645 0.68 +1.1 80,224 + EX31_RS17645 0.68 +1.9 80,225 - EX31_RS17645 0.68 +0.5 80,407 + EX31_RS17645 0.86 +0.3 80,418 + EX31_RS17645 0.87 +0.3 80,418 + EX31_RS17645 0.87 +1.9 80,419 - EX31_RS17645 0.87 +1.9 80,419 - EX31_RS17645 0.87 +0.5 80,419 - EX31_RS17645 0.87 -0.2 80,435 - EX31_RS17645 0.88 -0.1 80,435 - EX31_RS17645 0.88 +1.0 80,435 - EX31_RS17645 0.88 +1.2 80,553 + +0.9 80,553 + -0.4 80,554 - -0.3 80,554 - -0.1 80,554 - +0.9 80,554 - -0.5 80,554 - -0.5 80,586 + -0.3 80,586 + -1.1 80,586 + +0.2 80,586 + +0.3 80,586 + -0.9 80,586 + +1.5 80,587 - +1.3 80,596 + +0.1 80,596 + +2.9 80,596 + -0.5 80,599 - -0.7 80,599 - +0.3 80,616 + +0.4 80,616 + +1.8 80,617 - +0.1 80,617 - -1.6 80,639 + +2.5 80,640 - -1.0 80,665 + +0.6 80,665 + +0.6 80,665 + +0.5 80,666 - -1.8 80,799 + EX31_RS17650 0.16 -0.1 80,800 - EX31_RS17650 0.16 +1.4 80,812 + EX31_RS17650 0.17 -1.1 80,812 + EX31_RS17650 0.17 +0.9 80,813 - EX31_RS17650 0.17 -2.0 80,813 - EX31_RS17650 0.17 +1.1 80,813 - EX31_RS17650 0.17 -0.3 80,980 - EX31_RS17650 0.36 +1.3 81,025 + EX31_RS17650 0.41 -0.1 81,136 + EX31_RS17650 0.53 -0.0 81,169 + EX31_RS17650 0.57 +0.5 81,246 + EX31_RS17650 0.66 -1.1 81,271 + EX31_RS17650 0.68 +0.2 81,286 + EX31_RS17650 0.70 +1.9 81,286 + EX31_RS17650 0.70 -0.2 81,286 + EX31_RS17650 0.70 -0.4 81,287 - EX31_RS17650 0.70 -0.7 81,287 - EX31_RS17650 0.70 -0.5 81,289 + EX31_RS17650 0.70 +0.6 81,290 - EX31_RS17650 0.71 +1.3 81,307 + EX31_RS17650 0.72 +1.3 81,324 + EX31_RS17650 0.74 -0.9 81,324 + EX31_RS17650 0.74 +0.1 81,324 + EX31_RS17650 0.74 -0.9 81,367 - EX31_RS17650 0.79 +0.8 81,367 - EX31_RS17650 0.79 +0.1 81,456 - EX31_RS17650 0.89 +1.0 81,473 - -0.8 81,504 + +0.9 81,505 - -1.1 81,549 + +0.5 81,549 + -0.6 81,549 + +0.1 81,550 - +1.9 81,550 - -0.5 81,550 - +0.3 81,550 - +0.9 81,550 - +0.6 81,550 - +0.5
Or see this region's nucleotide sequence