Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15575 and EX31_RS15580 are separated by 225 nucleotides EX31_RS15580 and EX31_RS15585 are separated by 36 nucleotides EX31_RS15585 and EX31_RS15590 overlap by 20 nucleotides
EX31_RS15575: EX31_RS15575 - LysR family transcriptional regulator, at 10,081 to 10,956
_RS15575
EX31_RS15580: EX31_RS15580 - FAD-dependent oxidoreductase, at 11,182 to 12,495
_RS15580
EX31_RS15585: EX31_RS15585 - ABC transporter permease, at 12,532 to 13,188
_RS15585
EX31_RS15590: EX31_RS15590 - methionine ABC transporter ATP-binding protein, at 13,169 to 14,323
_RS15590
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-1
0
1
2
3 at 10.472 kb on + strand, within EX31_RS15575 at 10.472 kb on + strand, within EX31_RS15575 at 10.472 kb on + strand, within EX31_RS15575 at 10.473 kb on - strand, within EX31_RS15575 at 10.473 kb on - strand, within EX31_RS15575 at 10.535 kb on + strand, within EX31_RS15575 at 10.535 kb on + strand, within EX31_RS15575 at 10.589 kb on + strand, within EX31_RS15575 at 10.635 kb on - strand, within EX31_RS15575 at 10.635 kb on - strand, within EX31_RS15575 at 10.812 kb on - strand, within EX31_RS15575 at 10.812 kb on - strand, within EX31_RS15575 at 10.812 kb on - strand, within EX31_RS15575 at 10.863 kb on + strand, within EX31_RS15575 at 10.863 kb on + strand, within EX31_RS15575 at 10.864 kb on - strand, within EX31_RS15575 at 10.864 kb on - strand, within EX31_RS15575 at 11.122 kb on - strand at 11.129 kb on + strand at 11.130 kb on - strand at 11.130 kb on - strand at 11.131 kb on + strand at 11.132 kb on - strand at 11.198 kb on + strand at 11.198 kb on + strand at 11.214 kb on + strand at 11.264 kb on + strand at 11.264 kb on + strand at 11.264 kb on + strand at 11.334 kb on + strand, within EX31_RS15580 at 11.409 kb on + strand, within EX31_RS15580 at 11.432 kb on + strand, within EX31_RS15580 at 11.495 kb on + strand, within EX31_RS15580 at 11.495 kb on + strand, within EX31_RS15580 at 11.496 kb on - strand, within EX31_RS15580 at 11.572 kb on + strand, within EX31_RS15580 at 11.572 kb on + strand, within EX31_RS15580 at 11.573 kb on - strand, within EX31_RS15580 at 11.617 kb on + strand, within EX31_RS15580 at 11.730 kb on + strand, within EX31_RS15580 at 11.731 kb on - strand, within EX31_RS15580 at 11.798 kb on - strand, within EX31_RS15580 at 12.062 kb on - strand, within EX31_RS15580 at 12.063 kb on + strand, within EX31_RS15580 at 12.064 kb on - strand, within EX31_RS15580 at 12.064 kb on - strand, within EX31_RS15580 at 12.177 kb on + strand, within EX31_RS15580 at 12.178 kb on - strand, within EX31_RS15580 at 12.204 kb on + strand, within EX31_RS15580 at 12.211 kb on + strand, within EX31_RS15580 at 12.212 kb on - strand, within EX31_RS15580 at 12.238 kb on - strand, within EX31_RS15580 at 12.268 kb on - strand, within EX31_RS15580 at 12.284 kb on - strand, within EX31_RS15580 at 12.457 kb on - strand at 12.619 kb on + strand, within EX31_RS15585 at 12.619 kb on + strand, within EX31_RS15585 at 12.619 kb on + strand, within EX31_RS15585 at 12.619 kb on + strand, within EX31_RS15585 at 12.620 kb on - strand, within EX31_RS15585 at 12.648 kb on - strand, within EX31_RS15585 at 12.753 kb on + strand, within EX31_RS15585 at 12.801 kb on + strand, within EX31_RS15585 at 12.985 kb on - strand, within EX31_RS15585 at 13.188 kb on + strand at 13.188 kb on + strand at 13.189 kb on - strand at 13.297 kb on - strand, within EX31_RS15590 at 13.371 kb on - strand, within EX31_RS15590 at 13.410 kb on + strand, within EX31_RS15590 at 13.411 kb on - strand, within EX31_RS15590 at 13.475 kb on + strand, within EX31_RS15590 at 13.476 kb on - strand, within EX31_RS15590 at 13.484 kb on + strand, within EX31_RS15590
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 10,472 + EX31_RS15575 0.45 -0.4 10,472 + EX31_RS15575 0.45 -0.9 10,472 + EX31_RS15575 0.45 -0.6 10,473 - EX31_RS15575 0.45 -1.2 10,473 - EX31_RS15575 0.45 -0.7 10,535 + EX31_RS15575 0.52 +1.6 10,535 + EX31_RS15575 0.52 +0.8 10,589 + EX31_RS15575 0.58 -0.6 10,635 - EX31_RS15575 0.63 +0.5 10,635 - EX31_RS15575 0.63 +0.2 10,812 - EX31_RS15575 0.83 -1.4 10,812 - EX31_RS15575 0.83 -0.3 10,812 - EX31_RS15575 0.83 -0.8 10,863 + EX31_RS15575 0.89 -1.6 10,863 + EX31_RS15575 0.89 +0.5 10,864 - EX31_RS15575 0.89 +0.3 10,864 - EX31_RS15575 0.89 +1.0 11,122 - +1.0 11,129 + -1.6 11,130 - +0.9 11,130 - -0.1 11,131 + -0.8 11,132 - +1.2 11,198 + -0.2 11,198 + -0.8 11,214 + -0.2 11,264 + +1.1 11,264 + +1.5 11,264 + -0.2 11,334 + EX31_RS15580 0.12 +1.1 11,409 + EX31_RS15580 0.17 -0.1 11,432 + EX31_RS15580 0.19 +2.8 11,495 + EX31_RS15580 0.24 -0.9 11,495 + EX31_RS15580 0.24 +1.1 11,496 - EX31_RS15580 0.24 -0.7 11,572 + EX31_RS15580 0.30 +0.2 11,572 + EX31_RS15580 0.30 +1.9 11,573 - EX31_RS15580 0.30 +1.9 11,617 + EX31_RS15580 0.33 +0.5 11,730 + EX31_RS15580 0.42 +1.0 11,731 - EX31_RS15580 0.42 +0.9 11,798 - EX31_RS15580 0.47 -1.1 12,062 - EX31_RS15580 0.67 +0.4 12,063 + EX31_RS15580 0.67 +1.8 12,064 - EX31_RS15580 0.67 +0.7 12,064 - EX31_RS15580 0.67 +1.5 12,177 + EX31_RS15580 0.76 +0.8 12,178 - EX31_RS15580 0.76 +1.2 12,204 + EX31_RS15580 0.78 +0.9 12,211 + EX31_RS15580 0.78 +0.9 12,212 - EX31_RS15580 0.78 -0.1 12,238 - EX31_RS15580 0.80 +0.3 12,268 - EX31_RS15580 0.83 +0.8 12,284 - EX31_RS15580 0.84 -0.0 12,457 - +0.8 12,619 + EX31_RS15585 0.13 -1.4 12,619 + EX31_RS15585 0.13 -0.2 12,619 + EX31_RS15585 0.13 -0.6 12,619 + EX31_RS15585 0.13 -0.1 12,620 - EX31_RS15585 0.13 +0.3 12,648 - EX31_RS15585 0.18 +1.8 12,753 + EX31_RS15585 0.34 -0.4 12,801 + EX31_RS15585 0.41 +0.8 12,985 - EX31_RS15585 0.69 -1.2 13,188 + +0.7 13,188 + +0.0 13,189 - +1.2 13,297 - EX31_RS15590 0.11 -0.4 13,371 - EX31_RS15590 0.17 -0.8 13,410 + EX31_RS15590 0.21 -1.2 13,411 - EX31_RS15590 0.21 +0.0 13,475 + EX31_RS15590 0.26 -1.1 13,476 - EX31_RS15590 0.27 -0.2 13,484 + EX31_RS15590 0.27 -0.8
Or see this region's nucleotide sequence