Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11245 and EX31_RS11250 are separated by 111 nucleotides EX31_RS11250 and EX31_RS11255 are separated by 389 nucleotides EX31_RS11255 and EX31_RS11260 overlap by 8 nucleotides
EX31_RS11245: EX31_RS11245 - amino acid ABC transporter permease, at 181,847 to 182,761
_RS11245
EX31_RS11250: EX31_RS11250 - amino acid ABC transporter ATP-binding protein, at 182,873 to 183,622
_RS11250
EX31_RS11255: EX31_RS11255 - CidA/LrgA family protein, at 184,012 to 184,416
_RS11255
EX31_RS11260: EX31_RS11260 - CidB/LrgB family autolysis modulator, at 184,409 to 185,104
_RS11260
Position (kb)
182
183
184 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 182.002 kb on - strand, within EX31_RS11245 at 182.002 kb on - strand, within EX31_RS11245 at 182.137 kb on - strand, within EX31_RS11245 at 182.137 kb on - strand, within EX31_RS11245 at 182.159 kb on + strand, within EX31_RS11245 at 182.160 kb on - strand, within EX31_RS11245 at 182.161 kb on + strand, within EX31_RS11245 at 182.228 kb on + strand, within EX31_RS11245 at 182.228 kb on + strand, within EX31_RS11245 at 182.385 kb on + strand, within EX31_RS11245 at 182.469 kb on + strand, within EX31_RS11245 at 182.470 kb on - strand, within EX31_RS11245 at 182.534 kb on + strand, within EX31_RS11245 at 182.534 kb on + strand, within EX31_RS11245 at 182.535 kb on - strand, within EX31_RS11245 at 182.535 kb on - strand, within EX31_RS11245 at 182.535 kb on - strand, within EX31_RS11245 at 182.535 kb on - strand, within EX31_RS11245 at 182.562 kb on - strand, within EX31_RS11245 at 182.580 kb on - strand, within EX31_RS11245 at 182.580 kb on - strand, within EX31_RS11245 at 182.631 kb on - strand, within EX31_RS11245 at 182.631 kb on - strand, within EX31_RS11245 at 182.667 kb on - strand, within EX31_RS11245 at 182.669 kb on + strand, within EX31_RS11245 at 182.669 kb on + strand, within EX31_RS11245 at 182.669 kb on + strand, within EX31_RS11245 at 182.670 kb on - strand at 182.670 kb on - strand at 182.670 kb on - strand at 182.670 kb on - strand at 182.670 kb on - strand at 182.681 kb on + strand at 182.681 kb on + strand at 182.682 kb on - strand at 182.759 kb on + strand at 182.776 kb on + strand at 182.776 kb on + strand at 182.776 kb on + strand at 182.776 kb on + strand at 182.777 kb on - strand at 182.777 kb on - strand at 182.885 kb on - strand at 182.897 kb on + strand at 182.897 kb on + strand at 182.898 kb on - strand at 183.040 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.060 kb on - strand, within EX31_RS11250 at 183.060 kb on - strand, within EX31_RS11250 at 183.140 kb on + strand, within EX31_RS11250 at 183.162 kb on + strand, within EX31_RS11250 at 183.365 kb on - strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.523 kb on + strand, within EX31_RS11250 at 183.621 kb on - strand at 183.697 kb on + strand at 183.810 kb on - strand at 183.934 kb on - strand at 183.934 kb on - strand at 183.971 kb on + strand at 183.972 kb on - strand at 183.978 kb on - strand at 183.986 kb on + strand at 183.987 kb on - strand at 184.006 kb on + strand at 184.007 kb on - strand at 184.043 kb on - strand at 184.066 kb on + strand, within EX31_RS11255 at 184.106 kb on + strand, within EX31_RS11255 at 184.203 kb on + strand, within EX31_RS11255 at 184.203 kb on + strand, within EX31_RS11255 at 184.204 kb on - strand, within EX31_RS11255 at 184.204 kb on - strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.220 kb on - strand, within EX31_RS11255 at 184.221 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.268 kb on - strand, within EX31_RS11255 at 184.320 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.332 kb on - strand, within EX31_RS11255 at 184.348 kb on + strand, within EX31_RS11255 at 184.348 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.363 kb on - strand, within EX31_RS11255 at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.418 kb on - strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.477 kb on + strand at 184.477 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 182,002 - EX31_RS11245 0.17 -1.4 182,002 - EX31_RS11245 0.17 +0.4 182,137 - EX31_RS11245 0.32 -0.3 182,137 - EX31_RS11245 0.32 +0.7 182,159 + EX31_RS11245 0.34 +1.4 182,160 - EX31_RS11245 0.34 -0.2 182,161 + EX31_RS11245 0.34 +0.1 182,228 + EX31_RS11245 0.42 -0.4 182,228 + EX31_RS11245 0.42 +0.6 182,385 + EX31_RS11245 0.59 +1.2 182,469 + EX31_RS11245 0.68 +0.2 182,470 - EX31_RS11245 0.68 -0.9 182,534 + EX31_RS11245 0.75 +1.4 182,534 + EX31_RS11245 0.75 +0.7 182,535 - EX31_RS11245 0.75 -0.6 182,535 - EX31_RS11245 0.75 +0.5 182,535 - EX31_RS11245 0.75 +2.1 182,535 - EX31_RS11245 0.75 +0.8 182,562 - EX31_RS11245 0.78 +1.3 182,580 - EX31_RS11245 0.80 +0.2 182,580 - EX31_RS11245 0.80 +1.5 182,631 - EX31_RS11245 0.86 -0.0 182,631 - EX31_RS11245 0.86 +0.4 182,667 - EX31_RS11245 0.90 +0.5 182,669 + EX31_RS11245 0.90 +0.5 182,669 + EX31_RS11245 0.90 -1.6 182,669 + EX31_RS11245 0.90 +0.2 182,670 - +1.7 182,670 - +2.7 182,670 - +0.8 182,670 - +0.0 182,670 - -0.8 182,681 + -3.1 182,681 + +0.7 182,682 - -0.7 182,759 + -1.9 182,776 + +0.3 182,776 + +1.1 182,776 + -0.8 182,776 + +0.7 182,777 - -0.3 182,777 - +0.1 182,885 - -0.1 182,897 + -2.6 182,897 + -0.6 182,898 - +0.5 183,040 + EX31_RS11250 0.22 +0.8 183,059 + EX31_RS11250 0.25 -0.1 183,059 + EX31_RS11250 0.25 +0.0 183,059 + EX31_RS11250 0.25 +2.3 183,060 - EX31_RS11250 0.25 +0.0 183,060 - EX31_RS11250 0.25 +1.3 183,140 + EX31_RS11250 0.36 +1.5 183,162 + EX31_RS11250 0.39 -0.7 183,365 - EX31_RS11250 0.66 +0.6 183,436 + EX31_RS11250 0.75 -0.8 183,436 + EX31_RS11250 0.75 -0.4 183,436 + EX31_RS11250 0.75 -1.4 183,436 + EX31_RS11250 0.75 +0.4 183,523 + EX31_RS11250 0.87 -0.9 183,621 - +0.7 183,697 + -0.1 183,810 - +1.2 183,934 - -0.7 183,934 - +0.8 183,971 + +1.7 183,972 - +0.3 183,978 - -0.1 183,986 + +0.9 183,987 - +0.9 184,006 + +1.2 184,007 - -0.6 184,043 - -0.6 184,066 + EX31_RS11255 0.13 -1.1 184,106 + EX31_RS11255 0.23 -0.1 184,203 + EX31_RS11255 0.47 -0.1 184,203 + EX31_RS11255 0.47 -0.3 184,204 - EX31_RS11255 0.47 +0.7 184,204 - EX31_RS11255 0.47 -0.1 184,219 + EX31_RS11255 0.51 +0.3 184,219 + EX31_RS11255 0.51 +0.4 184,219 + EX31_RS11255 0.51 -1.2 184,219 + EX31_RS11255 0.51 +0.0 184,220 - EX31_RS11255 0.51 -0.6 184,221 + EX31_RS11255 0.52 -0.1 184,264 + EX31_RS11255 0.62 +0.4 184,264 + EX31_RS11255 0.62 +1.5 184,264 + EX31_RS11255 0.62 +0.6 184,268 - EX31_RS11255 0.63 +0.2 184,320 + EX31_RS11255 0.76 +0.2 184,331 + EX31_RS11255 0.79 +0.5 184,331 + EX31_RS11255 0.79 +0.2 184,331 + EX31_RS11255 0.79 +0.4 184,332 - EX31_RS11255 0.79 -1.0 184,348 + EX31_RS11255 0.83 +1.9 184,348 + EX31_RS11255 0.83 -0.8 184,360 + EX31_RS11255 0.86 -0.7 184,360 + EX31_RS11255 0.86 +0.1 184,360 + EX31_RS11255 0.86 +1.5 184,360 + EX31_RS11255 0.86 +1.6 184,360 + EX31_RS11255 0.86 -1.3 184,362 + EX31_RS11255 0.86 +0.6 184,362 + EX31_RS11255 0.86 -0.4 184,362 + EX31_RS11255 0.86 -0.8 184,362 + EX31_RS11255 0.86 +0.8 184,363 - EX31_RS11255 0.87 -1.4 184,417 + +0.0 184,417 + -1.1 184,417 + +0.6 184,417 + +0.9 184,417 + -1.1 184,417 + -0.4 184,417 + -0.8 184,417 + -0.1 184,417 + +0.0 184,418 - +0.3 184,419 + +0.6 184,419 + +0.1 184,419 + -0.5 184,419 + +1.7 184,419 + +0.3 184,419 + -0.5 184,419 + -0.4 184,419 + +1.3 184,420 - +2.7 184,420 - -0.3 184,420 - +0.6 184,477 + +0.4 184,477 + +0.8
Or see this region's nucleotide sequence