Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08730 and EX31_RS08735 are separated by 65 nucleotides EX31_RS08735 and EX31_RS08740 are separated by 255 nucleotides EX31_RS08740 and EX31_RS08745 are separated by 87 nucleotides EX31_RS08745 and EX31_RS08750 are separated by 39 nucleotides EX31_RS08750 and EX31_RS25265 are separated by 4 nucleotides
EX31_RS08730: EX31_RS08730 - iron-sulfur cluster repair protein YtfE, at 256,494 to 257,159
_RS08730
EX31_RS08735: EX31_RS08735 - peptidylprolyl isomerase, at 257,225 to 257,845
_RS08735
EX31_RS08740: EX31_RS08740 - opacity-associated protein OapA, at 258,101 to 258,811
_RS08740
EX31_RS08745: EX31_RS08745 - 50S ribosomal protein L9, at 258,899 to 259,351
_RS08745
EX31_RS08750: EX31_RS08750 - 30S ribosomal protein S18, at 259,391 to 259,618
_RS08750
EX31_RS25265: EX31_RS25265 - primosomal replication protein N, at 259,623 to 259,940
_RS25265
Position (kb) 258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 257.157 kb on + strand at 257.175 kb on + strand at 257.189 kb on + strand at 257.189 kb on + strand at 257.190 kb on - strand at 257.190 kb on - strand at 257.190 kb on - strand at 257.226 kb on + strand at 257.268 kb on + strand at 257.268 kb on + strand at 257.268 kb on + strand at 257.269 kb on - strand at 257.269 kb on - strand at 257.323 kb on + strand, within EX31_RS08735 at 257.324 kb on - strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.325 kb on + strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.326 kb on - strand, within EX31_RS08735 at 257.339 kb on - strand, within EX31_RS08735 at 257.339 kb on - strand, within EX31_RS08735 at 257.423 kb on + strand, within EX31_RS08735 at 257.466 kb on + strand, within EX31_RS08735 at 257.477 kb on + strand, within EX31_RS08735 at 257.478 kb on - strand, within EX31_RS08735 at 257.478 kb on - strand, within EX31_RS08735 at 257.478 kb on - strand, within EX31_RS08735 at 257.478 kb on - strand, within EX31_RS08735 at 257.478 kb on - strand, within EX31_RS08735 at 257.721 kb on - strand, within EX31_RS08735 at 257.748 kb on - strand, within EX31_RS08735 at 257.802 kb on + strand at 257.803 kb on - strand at 257.803 kb on - strand at 257.803 kb on - strand at 257.803 kb on - strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.819 kb on + strand at 257.820 kb on - strand at 257.820 kb on - strand at 257.820 kb on - strand at 257.820 kb on - strand at 257.820 kb on - strand at 257.820 kb on - strand at 257.856 kb on + strand at 257.857 kb on - strand at 257.885 kb on + strand at 257.885 kb on + strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.886 kb on - strand at 257.886 kb on - strand at 258.004 kb on + strand at 258.004 kb on + strand at 258.005 kb on - strand at 258.005 kb on - strand at 258.048 kb on + strand at 258.048 kb on + strand at 258.048 kb on + strand at 258.048 kb on + strand at 258.049 kb on - strand at 258.049 kb on - strand at 258.049 kb on - strand at 258.049 kb on - strand at 258.051 kb on + strand at 258.051 kb on + strand at 258.051 kb on + strand at 258.051 kb on + strand at 258.051 kb on + strand at 258.052 kb on - strand at 258.052 kb on - strand at 258.052 kb on - strand at 258.052 kb on - strand at 258.052 kb on - strand at 258.052 kb on - strand at 258.075 kb on + strand at 258.084 kb on + strand at 258.084 kb on + strand at 258.084 kb on + strand at 258.085 kb on - strand at 258.085 kb on - strand at 258.139 kb on + strand at 258.139 kb on + strand at 258.139 kb on + strand at 258.139 kb on + strand at 258.143 kb on + strand at 258.143 kb on + strand at 258.143 kb on + strand at 258.143 kb on + strand at 258.143 kb on + strand at 258.144 kb on - strand at 258.175 kb on + strand, within EX31_RS08740 at 258.256 kb on + strand, within EX31_RS08740 at 258.256 kb on + strand, within EX31_RS08740 at 258.342 kb on - strand, within EX31_RS08740 at 258.361 kb on + strand, within EX31_RS08740 at 258.393 kb on + strand, within EX31_RS08740 at 258.393 kb on + strand, within EX31_RS08740 at 258.393 kb on + strand, within EX31_RS08740 at 258.394 kb on - strand, within EX31_RS08740 at 258.404 kb on + strand, within EX31_RS08740 at 258.404 kb on + strand, within EX31_RS08740 at 258.404 kb on + strand, within EX31_RS08740 at 258.404 kb on + strand, within EX31_RS08740 at 258.404 kb on + strand, within EX31_RS08740 at 258.405 kb on - strand, within EX31_RS08740 at 258.405 kb on - strand, within EX31_RS08740 at 258.406 kb on + strand, within EX31_RS08740 at 258.406 kb on + strand, within EX31_RS08740 at 258.407 kb on - strand, within EX31_RS08740 at 258.407 kb on - strand, within EX31_RS08740 at 258.422 kb on + strand, within EX31_RS08740 at 258.422 kb on + strand, within EX31_RS08740 at 258.422 kb on + strand, within EX31_RS08740 at 258.422 kb on + strand, within EX31_RS08740 at 258.423 kb on - strand, within EX31_RS08740 at 258.423 kb on - strand, within EX31_RS08740 at 258.423 kb on - strand, within EX31_RS08740 at 258.423 kb on - strand, within EX31_RS08740 at 258.455 kb on - strand, within EX31_RS08740 at 258.535 kb on + strand, within EX31_RS08740 at 258.536 kb on - strand, within EX31_RS08740 at 258.563 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.610 kb on + strand, within EX31_RS08740 at 258.611 kb on - strand, within EX31_RS08740 at 258.611 kb on - strand, within EX31_RS08740 at 258.611 kb on - strand, within EX31_RS08740 at 258.613 kb on + strand, within EX31_RS08740 at 258.614 kb on - strand, within EX31_RS08740 at 258.614 kb on - strand, within EX31_RS08740 at 258.622 kb on - strand, within EX31_RS08740 at 258.630 kb on + strand, within EX31_RS08740 at 258.648 kb on + strand, within EX31_RS08740 at 258.648 kb on + strand, within EX31_RS08740 at 258.673 kb on + strand, within EX31_RS08740 at 258.673 kb on + strand, within EX31_RS08740 at 258.674 kb on - strand, within EX31_RS08740 at 258.674 kb on - strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.702 kb on + strand, within EX31_RS08740 at 258.703 kb on - strand, within EX31_RS08740 at 258.760 kb on + strand at 258.773 kb on - strand at 258.806 kb on - strand at 258.821 kb on + strand at 258.821 kb on + strand at 258.886 kb on - strand at 258.921 kb on - strand at 258.921 kb on - strand at 258.921 kb on - strand at 258.921 kb on - strand at 258.921 kb on - strand at 258.939 kb on - strand at 259.019 kb on - strand, within EX31_RS08745 at 259.019 kb on - strand, within EX31_RS08745 at 259.073 kb on - strand, within EX31_RS08745 at 259.119 kb on - strand, within EX31_RS08745 at 259.378 kb on - strand at 259.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 257,157 + -0.5 257,175 + +1.3 257,189 + -0.9 257,189 + -0.6 257,190 - +0.5 257,190 - +0.1 257,190 - -1.1 257,226 + -0.2 257,268 + -0.4 257,268 + +1.0 257,268 + -0.0 257,269 - +0.2 257,269 - -0.9 257,323 + EX31_RS08735 0.16 +0.2 257,324 - EX31_RS08735 0.16 -0.6 257,325 + EX31_RS08735 0.16 -0.0 257,325 + EX31_RS08735 0.16 +0.1 257,325 + EX31_RS08735 0.16 -1.3 257,325 + EX31_RS08735 0.16 +1.5 257,325 + EX31_RS08735 0.16 -0.9 257,325 + EX31_RS08735 0.16 +0.5 257,325 + EX31_RS08735 0.16 +0.9 257,325 + EX31_RS08735 0.16 -0.3 257,325 + EX31_RS08735 0.16 +0.5 257,325 + EX31_RS08735 0.16 +1.9 257,325 + EX31_RS08735 0.16 -0.1 257,325 + EX31_RS08735 0.16 -0.4 257,325 + EX31_RS08735 0.16 +0.5 257,325 + EX31_RS08735 0.16 +1.2 257,325 + EX31_RS08735 0.16 +0.7 257,325 + EX31_RS08735 0.16 -0.7 257,325 + EX31_RS08735 0.16 +0.3 257,325 + EX31_RS08735 0.16 -0.0 257,325 + EX31_RS08735 0.16 -0.9 257,325 + EX31_RS08735 0.16 -1.4 257,326 - EX31_RS08735 0.16 -2.6 257,326 - EX31_RS08735 0.16 -0.6 257,326 - EX31_RS08735 0.16 +0.9 257,326 - EX31_RS08735 0.16 -0.7 257,326 - EX31_RS08735 0.16 +0.4 257,326 - EX31_RS08735 0.16 -0.1 257,326 - EX31_RS08735 0.16 +0.7 257,326 - EX31_RS08735 0.16 -0.2 257,326 - EX31_RS08735 0.16 +0.2 257,326 - EX31_RS08735 0.16 +0.9 257,326 - EX31_RS08735 0.16 -1.1 257,326 - EX31_RS08735 0.16 -0.3 257,326 - EX31_RS08735 0.16 +0.2 257,326 - EX31_RS08735 0.16 -0.2 257,326 - EX31_RS08735 0.16 -1.7 257,326 - EX31_RS08735 0.16 -0.0 257,326 - EX31_RS08735 0.16 +0.1 257,326 - EX31_RS08735 0.16 -0.3 257,326 - EX31_RS08735 0.16 -1.9 257,326 - EX31_RS08735 0.16 +1.2 257,326 - EX31_RS08735 0.16 +0.4 257,326 - EX31_RS08735 0.16 -1.2 257,326 - EX31_RS08735 0.16 -0.1 257,326 - EX31_RS08735 0.16 -0.2 257,326 - EX31_RS08735 0.16 +0.1 257,326 - EX31_RS08735 0.16 +1.2 257,326 - EX31_RS08735 0.16 +1.8 257,326 - EX31_RS08735 0.16 -0.2 257,326 - EX31_RS08735 0.16 -0.7 257,339 - EX31_RS08735 0.18 +0.3 257,339 - EX31_RS08735 0.18 +0.5 257,423 + EX31_RS08735 0.32 +0.4 257,466 + EX31_RS08735 0.39 +0.1 257,477 + EX31_RS08735 0.41 +0.5 257,478 - EX31_RS08735 0.41 +0.1 257,478 - EX31_RS08735 0.41 +0.1 257,478 - EX31_RS08735 0.41 -1.2 257,478 - EX31_RS08735 0.41 -0.1 257,478 - EX31_RS08735 0.41 +0.2 257,721 - EX31_RS08735 0.80 -0.1 257,748 - EX31_RS08735 0.84 +0.2 257,802 + +1.5 257,803 - -1.3 257,803 - -0.5 257,803 - +0.1 257,803 - -0.1 257,819 + +0.4 257,819 + -1.4 257,819 + +0.9 257,819 + +1.1 257,819 + +0.2 257,819 + -0.1 257,819 + -1.3 257,819 + -1.3 257,819 + -1.4 257,820 - +0.7 257,820 - -1.4 257,820 - -1.6 257,820 - +0.3 257,820 - -0.7 257,820 - -0.7 257,856 + -0.2 257,857 - +0.1 257,885 + +0.2 257,885 + +0.1 257,886 - +0.4 257,886 - +1.4 257,886 - -0.0 257,886 - -1.8 257,886 - +0.1 257,886 - +2.1 258,004 + +0.7 258,004 + -0.3 258,005 - +0.0 258,005 - +0.8 258,048 + -0.0 258,048 + -0.4 258,048 + +0.5 258,048 + +0.4 258,049 - -0.2 258,049 - +0.1 258,049 - +0.1 258,049 - -0.8 258,051 + -0.3 258,051 + +0.3 258,051 + -0.6 258,051 + +0.0 258,051 + +0.1 258,052 - +1.3 258,052 - -0.4 258,052 - +0.9 258,052 - +0.8 258,052 - -0.4 258,052 - +0.4 258,075 + +0.0 258,084 + -0.2 258,084 + -0.5 258,084 + +0.1 258,085 - -0.6 258,085 - -0.4 258,139 + -0.2 258,139 + +0.9 258,139 + +0.7 258,139 + +0.4 258,143 + +1.6 258,143 + -0.2 258,143 + +0.7 258,143 + +0.5 258,143 + -1.5 258,144 - +0.3 258,175 + EX31_RS08740 0.10 -0.1 258,256 + EX31_RS08740 0.22 -0.8 258,256 + EX31_RS08740 0.22 +0.6 258,342 - EX31_RS08740 0.34 +0.3 258,361 + EX31_RS08740 0.37 +0.9 258,393 + EX31_RS08740 0.41 -0.8 258,393 + EX31_RS08740 0.41 -0.1 258,393 + EX31_RS08740 0.41 +1.5 258,394 - EX31_RS08740 0.41 -0.2 258,404 + EX31_RS08740 0.43 -0.9 258,404 + EX31_RS08740 0.43 +0.1 258,404 + EX31_RS08740 0.43 -0.3 258,404 + EX31_RS08740 0.43 -0.7 258,404 + EX31_RS08740 0.43 -0.3 258,405 - EX31_RS08740 0.43 -0.7 258,405 - EX31_RS08740 0.43 -0.3 258,406 + EX31_RS08740 0.43 -1.8 258,406 + EX31_RS08740 0.43 +1.5 258,407 - EX31_RS08740 0.43 -0.9 258,407 - EX31_RS08740 0.43 +0.7 258,422 + EX31_RS08740 0.45 +0.4 258,422 + EX31_RS08740 0.45 +1.3 258,422 + EX31_RS08740 0.45 +0.0 258,422 + EX31_RS08740 0.45 -1.0 258,423 - EX31_RS08740 0.45 -0.8 258,423 - EX31_RS08740 0.45 -0.2 258,423 - EX31_RS08740 0.45 +0.2 258,423 - EX31_RS08740 0.45 -1.1 258,455 - EX31_RS08740 0.50 +0.3 258,535 + EX31_RS08740 0.61 +0.4 258,536 - EX31_RS08740 0.61 +0.6 258,563 + EX31_RS08740 0.65 +1.7 258,610 + EX31_RS08740 0.72 +0.2 258,610 + EX31_RS08740 0.72 -1.0 258,610 + EX31_RS08740 0.72 +0.3 258,610 + EX31_RS08740 0.72 +0.8 258,610 + EX31_RS08740 0.72 +0.2 258,610 + EX31_RS08740 0.72 +0.0 258,610 + EX31_RS08740 0.72 +0.5 258,610 + EX31_RS08740 0.72 +0.1 258,610 + EX31_RS08740 0.72 +0.8 258,610 + EX31_RS08740 0.72 -1.6 258,611 - EX31_RS08740 0.72 +0.7 258,611 - EX31_RS08740 0.72 +0.9 258,611 - EX31_RS08740 0.72 +0.2 258,613 + EX31_RS08740 0.72 +0.1 258,614 - EX31_RS08740 0.72 -0.4 258,614 - EX31_RS08740 0.72 +1.0 258,622 - EX31_RS08740 0.73 +0.6 258,630 + EX31_RS08740 0.74 +0.1 258,648 + EX31_RS08740 0.77 +1.2 258,648 + EX31_RS08740 0.77 -0.9 258,673 + EX31_RS08740 0.80 +0.5 258,673 + EX31_RS08740 0.80 -0.3 258,674 - EX31_RS08740 0.81 +1.3 258,674 - EX31_RS08740 0.81 +0.2 258,702 + EX31_RS08740 0.85 +0.8 258,702 + EX31_RS08740 0.85 +0.7 258,702 + EX31_RS08740 0.85 +0.2 258,702 + EX31_RS08740 0.85 -0.0 258,702 + EX31_RS08740 0.85 -0.2 258,702 + EX31_RS08740 0.85 +1.6 258,703 - EX31_RS08740 0.85 -0.2 258,760 + +1.7 258,773 - +0.7 258,806 - -1.1 258,821 + -0.0 258,821 + +0.8 258,886 - -0.9 258,921 - +0.2 258,921 - +0.2 258,921 - -0.5 258,921 - -0.7 258,921 - -0.1 258,939 - +1.5 259,019 - EX31_RS08745 0.26 -1.4 259,019 - EX31_RS08745 0.26 -0.9 259,073 - EX31_RS08745 0.38 +0.3 259,119 - EX31_RS08745 0.49 -2.0 259,378 - -0.1 259,378 - -1.1
Or see this region's nucleotide sequence