Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06305 and EX31_RS06310 are separated by 179 nucleotides EX31_RS06310 and EX31_RS06315 are separated by 220 nucleotides EX31_RS06315 and EX31_RS06320 overlap by 8 nucleotides EX31_RS06320 and EX31_RS06325 are separated by 74 nucleotides EX31_RS06325 and EX31_RS06330 overlap by 1 nucleotides
EX31_RS06305: EX31_RS06305 - signal peptide peptidase SppA, at 22,770 to 24,623
_RS06305
EX31_RS06310: EX31_RS06310 - NAD(P)H nitroreductase, at 24,803 to 25,354
_RS06310
EX31_RS06315: EX31_RS06315 - DNA topoisomerase III, at 25,575 to 27,527
_RS06315
EX31_RS06320: EX31_RS06320 - YnjH family protein, at 27,520 to 27,834
_RS06320
EX31_RS06325: EX31_RS06325 - pyrimidine (deoxy)nucleoside triphosphate diphosphatase, at 27,909 to 28,310
_RS06325
EX31_RS06330: EX31_RS06330 - exodeoxyribonuclease III, at 28,310 to 29,119
_RS06330
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 24.644 kb on + strand at 24.644 kb on + strand at 24.644 kb on + strand at 24.644 kb on + strand at 24.644 kb on + strand at 24.645 kb on - strand at 24.645 kb on - strand at 24.645 kb on - strand at 24.660 kb on - strand at 24.660 kb on - strand at 24.660 kb on - strand at 24.687 kb on + strand at 24.687 kb on + strand at 24.687 kb on + strand at 24.687 kb on + strand at 24.687 kb on + strand at 24.719 kb on - strand at 24.786 kb on - strand at 24.822 kb on + strand at 24.822 kb on + strand at 24.822 kb on + strand at 24.823 kb on - strand at 25.002 kb on + strand, within EX31_RS06310 at 25.070 kb on - strand, within EX31_RS06310 at 25.070 kb on - strand, within EX31_RS06310 at 25.102 kb on + strand, within EX31_RS06310 at 25.103 kb on - strand, within EX31_RS06310 at 25.198 kb on + strand, within EX31_RS06310 at 25.227 kb on + strand, within EX31_RS06310 at 25.227 kb on + strand, within EX31_RS06310 at 25.227 kb on + strand, within EX31_RS06310 at 25.228 kb on - strand, within EX31_RS06310 at 25.228 kb on - strand, within EX31_RS06310 at 25.228 kb on - strand, within EX31_RS06310 at 25.228 kb on - strand, within EX31_RS06310 at 25.278 kb on - strand, within EX31_RS06310 at 25.371 kb on + strand at 25.372 kb on - strand at 25.403 kb on - strand at 25.453 kb on - strand at 25.461 kb on + strand at 25.461 kb on + strand at 25.461 kb on + strand at 25.462 kb on - strand at 25.464 kb on + strand at 25.469 kb on - strand at 25.483 kb on + strand at 25.488 kb on + strand at 25.488 kb on + strand at 25.488 kb on + strand at 25.489 kb on - strand at 25.489 kb on - strand at 25.512 kb on + strand at 25.533 kb on - strand at 25.554 kb on + strand at 25.554 kb on + strand at 25.554 kb on + strand at 25.554 kb on + strand at 25.554 kb on + strand at 25.662 kb on + strand at 25.662 kb on + strand at 25.662 kb on + strand at 25.662 kb on + strand at 25.663 kb on - strand at 25.663 kb on - strand at 25.664 kb on + strand at 25.711 kb on + strand at 25.711 kb on + strand at 25.712 kb on - strand at 25.849 kb on + strand, within EX31_RS06315 at 25.849 kb on + strand, within EX31_RS06315 at 25.929 kb on + strand, within EX31_RS06315 at 26.012 kb on + strand, within EX31_RS06315 at 26.043 kb on - strand, within EX31_RS06315 at 26.102 kb on + strand, within EX31_RS06315 at 26.102 kb on + strand, within EX31_RS06315 at 26.102 kb on + strand, within EX31_RS06315 at 26.115 kb on + strand, within EX31_RS06315 at 26.115 kb on + strand, within EX31_RS06315 at 26.248 kb on + strand, within EX31_RS06315 at 26.248 kb on + strand, within EX31_RS06315 at 26.412 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.462 kb on + strand, within EX31_RS06315 at 26.463 kb on - strand, within EX31_RS06315 at 26.463 kb on - strand, within EX31_RS06315 at 26.519 kb on + strand, within EX31_RS06315 at 26.555 kb on + strand, within EX31_RS06315 at 26.555 kb on + strand, within EX31_RS06315 at 26.555 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.750 kb on + strand, within EX31_RS06315 at 26.751 kb on - strand, within EX31_RS06315 at 26.805 kb on + strand, within EX31_RS06315 at 26.805 kb on + strand, within EX31_RS06315 at 26.806 kb on - strand, within EX31_RS06315 at 27.050 kb on + strand, within EX31_RS06315 at 27.051 kb on - strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.060 kb on - strand, within EX31_RS06315 at 27.104 kb on + strand, within EX31_RS06315 at 27.142 kb on + strand, within EX31_RS06315 at 27.216 kb on + strand, within EX31_RS06315 at 27.217 kb on - strand, within EX31_RS06315 at 27.217 kb on - strand, within EX31_RS06315 at 27.218 kb on + strand, within EX31_RS06315 at 27.218 kb on + strand, within EX31_RS06315 at 27.219 kb on - strand, within EX31_RS06315 at 27.219 kb on - strand, within EX31_RS06315 at 27.477 kb on - strand at 27.495 kb on - strand at 27.621 kb on + strand, within EX31_RS06320 at 27.678 kb on + strand, within EX31_RS06320 at 27.678 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.709 kb on - strand, within EX31_RS06320 at 27.709 kb on - strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.727 kb on + strand, within EX31_RS06320 at 27.843 kb on + strand at 27.895 kb on - strand at 27.895 kb on - strand at 27.899 kb on - strand at 28.026 kb on - strand, within EX31_RS06325 at 28.072 kb on - strand, within EX31_RS06325 at 28.091 kb on + strand, within EX31_RS06325 at 28.092 kb on - strand, within EX31_RS06325 at 28.158 kb on + strand, within EX31_RS06325 at 28.158 kb on + strand, within EX31_RS06325 at 28.159 kb on - strand, within EX31_RS06325 at 28.159 kb on - strand, within EX31_RS06325 at 28.254 kb on + strand, within EX31_RS06325 at 28.255 kb on - strand, within EX31_RS06325 at 28.311 kb on + strand at 28.311 kb on + strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.378 kb on - strand at 28.378 kb on - strand at 28.378 kb on - strand at 28.439 kb on - strand, within EX31_RS06330 at 28.496 kb on - strand, within EX31_RS06330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 24,644 + +0.7 24,644 + +0.5 24,644 + -0.6 24,644 + -0.5 24,644 + -1.2 24,645 - -0.1 24,645 - -0.6 24,645 - -0.8 24,660 - +0.1 24,660 - +0.3 24,660 - -0.7 24,687 + -0.8 24,687 + -0.5 24,687 + -0.1 24,687 + +1.5 24,687 + +0.7 24,719 - -0.7 24,786 - +0.6 24,822 + -1.1 24,822 + +0.9 24,822 + +0.1 24,823 - -2.4 25,002 + EX31_RS06310 0.36 +0.3 25,070 - EX31_RS06310 0.48 +0.1 25,070 - EX31_RS06310 0.48 -1.1 25,102 + EX31_RS06310 0.54 -0.1 25,103 - EX31_RS06310 0.54 +1.9 25,198 + EX31_RS06310 0.72 +0.8 25,227 + EX31_RS06310 0.77 -1.4 25,227 + EX31_RS06310 0.77 +0.2 25,227 + EX31_RS06310 0.77 +0.5 25,228 - EX31_RS06310 0.77 +0.5 25,228 - EX31_RS06310 0.77 -0.5 25,228 - EX31_RS06310 0.77 -0.1 25,228 - EX31_RS06310 0.77 +0.8 25,278 - EX31_RS06310 0.86 -1.3 25,371 + +1.1 25,372 - +0.5 25,403 - +0.6 25,453 - +0.0 25,461 + +0.2 25,461 + -1.6 25,461 + -0.4 25,462 - -1.3 25,464 + +0.4 25,469 - -0.7 25,483 + -1.1 25,488 + -1.6 25,488 + +0.2 25,488 + -0.1 25,489 - -0.7 25,489 - +1.4 25,512 + -0.1 25,533 - +1.7 25,554 + -0.3 25,554 + -1.6 25,554 + +0.1 25,554 + +0.3 25,554 + +0.5 25,662 + -0.1 25,662 + +0.4 25,662 + -0.8 25,662 + -0.3 25,663 - -1.8 25,663 - -0.5 25,664 + +1.9 25,711 + +1.1 25,711 + -0.3 25,712 - +1.2 25,849 + EX31_RS06315 0.14 +0.0 25,849 + EX31_RS06315 0.14 -0.5 25,929 + EX31_RS06315 0.18 -1.1 26,012 + EX31_RS06315 0.22 -0.1 26,043 - EX31_RS06315 0.24 -0.5 26,102 + EX31_RS06315 0.27 +0.3 26,102 + EX31_RS06315 0.27 -1.1 26,102 + EX31_RS06315 0.27 -0.1 26,115 + EX31_RS06315 0.28 -0.1 26,115 + EX31_RS06315 0.28 +0.5 26,248 + EX31_RS06315 0.34 -0.1 26,248 + EX31_RS06315 0.34 -0.7 26,412 + EX31_RS06315 0.43 -0.1 26,462 + EX31_RS06315 0.45 -2.8 26,462 + EX31_RS06315 0.45 -0.0 26,462 + EX31_RS06315 0.45 -0.4 26,462 + EX31_RS06315 0.45 +0.3 26,462 + EX31_RS06315 0.45 -0.6 26,462 + EX31_RS06315 0.45 +0.6 26,463 - EX31_RS06315 0.45 +1.3 26,463 - EX31_RS06315 0.45 -0.8 26,519 + EX31_RS06315 0.48 -0.4 26,555 + EX31_RS06315 0.50 +0.9 26,555 + EX31_RS06315 0.50 -0.1 26,555 + EX31_RS06315 0.50 -0.2 26,680 + EX31_RS06315 0.57 -0.3 26,680 + EX31_RS06315 0.57 +0.3 26,680 + EX31_RS06315 0.57 -1.2 26,680 + EX31_RS06315 0.57 +1.5 26,750 + EX31_RS06315 0.60 +0.7 26,751 - EX31_RS06315 0.60 +1.0 26,805 + EX31_RS06315 0.63 -1.3 26,805 + EX31_RS06315 0.63 +1.1 26,806 - EX31_RS06315 0.63 +1.0 27,050 + EX31_RS06315 0.76 -0.1 27,051 - EX31_RS06315 0.76 -0.5 27,059 + EX31_RS06315 0.76 -0.5 27,059 + EX31_RS06315 0.76 +0.3 27,059 + EX31_RS06315 0.76 +0.5 27,059 + EX31_RS06315 0.76 -0.3 27,059 + EX31_RS06315 0.76 -0.6 27,059 + EX31_RS06315 0.76 -2.5 27,060 - EX31_RS06315 0.76 +0.9 27,104 + EX31_RS06315 0.78 +3.5 27,142 + EX31_RS06315 0.80 +0.5 27,216 + EX31_RS06315 0.84 +0.1 27,217 - EX31_RS06315 0.84 -1.4 27,217 - EX31_RS06315 0.84 -0.2 27,218 + EX31_RS06315 0.84 -0.5 27,218 + EX31_RS06315 0.84 +0.6 27,219 - EX31_RS06315 0.84 -0.1 27,219 - EX31_RS06315 0.84 +0.1 27,477 - +1.3 27,495 - -0.1 27,621 + EX31_RS06320 0.32 -1.3 27,678 + EX31_RS06320 0.50 +1.1 27,678 + EX31_RS06320 0.50 -1.0 27,708 + EX31_RS06320 0.60 +0.5 27,708 + EX31_RS06320 0.60 -0.2 27,708 + EX31_RS06320 0.60 +0.7 27,709 - EX31_RS06320 0.60 +0.7 27,709 - EX31_RS06320 0.60 -0.5 27,712 + EX31_RS06320 0.61 +0.5 27,712 + EX31_RS06320 0.61 +0.5 27,712 + EX31_RS06320 0.61 -0.1 27,712 + EX31_RS06320 0.61 -1.4 27,712 + EX31_RS06320 0.61 -0.3 27,727 + EX31_RS06320 0.66 +0.5 27,843 + +0.6 27,895 - +0.6 27,895 - +0.7 27,899 - -0.1 28,026 - EX31_RS06325 0.29 -0.5 28,072 - EX31_RS06325 0.41 -0.6 28,091 + EX31_RS06325 0.45 -1.2 28,092 - EX31_RS06325 0.46 -0.3 28,158 + EX31_RS06325 0.62 +1.5 28,158 + EX31_RS06325 0.62 -1.5 28,159 - EX31_RS06325 0.62 +0.3 28,159 - EX31_RS06325 0.62 +1.1 28,254 + EX31_RS06325 0.86 +0.9 28,255 - EX31_RS06325 0.86 -0.9 28,311 + +0.4 28,311 + +0.5 28,312 - +0.1 28,312 - +2.1 28,312 - -0.3 28,312 - +0.9 28,378 - -0.2 28,378 - +2.1 28,378 - -1.3 28,439 - EX31_RS06330 0.16 -0.2 28,496 - EX31_RS06330 0.23 -1.2
Or see this region's nucleotide sequence