Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01100 and CA265_RS01105 are separated by 29 nucleotides CA265_RS01105 and CA265_RS01110 are separated by 30 nucleotides
CA265_RS01100: CA265_RS01100 - transcriptional regulator, at 278,651 to 279,040
_RS01100
CA265_RS01105: CA265_RS01105 - hypothetical protein, at 279,070 to 280,968
_RS01105
CA265_RS01110: CA265_RS01110 - hypothetical protein, at 280,999 to 283,446
_RS01110
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 278.136 kb on - strand at 278.162 kb on - strand at 278.177 kb on + strand at 278.210 kb on + strand at 278.211 kb on - strand at 278.211 kb on - strand at 278.211 kb on - strand at 278.222 kb on + strand at 278.234 kb on + strand at 278.234 kb on + strand at 278.294 kb on - strand at 278.326 kb on - strand at 278.376 kb on + strand at 278.391 kb on + strand at 278.391 kb on + strand at 278.392 kb on - strand at 278.468 kb on - strand at 278.497 kb on + strand at 278.640 kb on - strand at 278.660 kb on - strand at 278.740 kb on + strand, within CA265_RS01100 at 278.838 kb on + strand, within CA265_RS01100 at 278.838 kb on + strand, within CA265_RS01100 at 278.838 kb on + strand, within CA265_RS01100 at 278.839 kb on - strand, within CA265_RS01100 at 278.839 kb on - strand, within CA265_RS01100 at 278.871 kb on - strand, within CA265_RS01100 at 278.872 kb on + strand, within CA265_RS01100 at 278.873 kb on - strand, within CA265_RS01100 at 278.913 kb on + strand, within CA265_RS01100 at 278.926 kb on - strand, within CA265_RS01100 at 278.930 kb on - strand, within CA265_RS01100 at 278.936 kb on + strand, within CA265_RS01100 at 278.958 kb on + strand, within CA265_RS01100 at 279.012 kb on + strand at 279.013 kb on - strand at 279.039 kb on - strand at 279.192 kb on + strand at 279.260 kb on + strand, within CA265_RS01105 at 279.266 kb on + strand, within CA265_RS01105 at 279.267 kb on - strand, within CA265_RS01105 at 279.267 kb on - strand, within CA265_RS01105 at 279.422 kb on - strand, within CA265_RS01105 at 279.438 kb on - strand, within CA265_RS01105 at 279.485 kb on + strand, within CA265_RS01105 at 279.500 kb on + strand, within CA265_RS01105 at 279.500 kb on + strand, within CA265_RS01105 at 279.501 kb on - strand, within CA265_RS01105 at 279.501 kb on - strand, within CA265_RS01105 at 279.501 kb on - strand, within CA265_RS01105 at 279.503 kb on - strand, within CA265_RS01105 at 279.519 kb on + strand, within CA265_RS01105 at 279.520 kb on - strand, within CA265_RS01105 at 279.520 kb on - strand, within CA265_RS01105 at 279.585 kb on - strand, within CA265_RS01105 at 279.671 kb on + strand, within CA265_RS01105 at 279.752 kb on - strand, within CA265_RS01105 at 279.759 kb on - strand, within CA265_RS01105 at 279.767 kb on + strand, within CA265_RS01105 at 279.879 kb on - strand, within CA265_RS01105 at 279.946 kb on + strand, within CA265_RS01105 at 279.947 kb on - strand, within CA265_RS01105 at 279.947 kb on - strand, within CA265_RS01105 at 280.046 kb on + strand, within CA265_RS01105 at 280.047 kb on - strand, within CA265_RS01105 at 280.050 kb on - strand, within CA265_RS01105 at 280.092 kb on + strand, within CA265_RS01105 at 280.137 kb on + strand, within CA265_RS01105 at 280.161 kb on + strand, within CA265_RS01105 at 280.253 kb on + strand, within CA265_RS01105 at 280.341 kb on + strand, within CA265_RS01105 at 280.352 kb on + strand, within CA265_RS01105 at 280.383 kb on + strand, within CA265_RS01105 at 280.455 kb on + strand, within CA265_RS01105 at 280.478 kb on + strand, within CA265_RS01105 at 280.515 kb on + strand, within CA265_RS01105 at 280.587 kb on + strand, within CA265_RS01105 at 280.587 kb on + strand, within CA265_RS01105 at 280.588 kb on - strand, within CA265_RS01105 at 280.591 kb on + strand, within CA265_RS01105 at 280.591 kb on + strand, within CA265_RS01105 at 280.591 kb on + strand, within CA265_RS01105 at 280.591 kb on + strand, within CA265_RS01105 at 280.591 kb on + strand, within CA265_RS01105 at 280.592 kb on - strand, within CA265_RS01105 at 280.592 kb on - strand, within CA265_RS01105 at 280.592 kb on - strand, within CA265_RS01105 at 280.642 kb on - strand, within CA265_RS01105 at 280.681 kb on + strand, within CA265_RS01105 at 280.681 kb on + strand, within CA265_RS01105 at 280.719 kb on + strand, within CA265_RS01105 at 280.726 kb on - strand, within CA265_RS01105 at 280.739 kb on + strand, within CA265_RS01105 at 280.740 kb on - strand, within CA265_RS01105 at 280.803 kb on + strand at 280.807 kb on - strand at 280.828 kb on + strand at 280.937 kb on - strand at 280.966 kb on + strand at 281.005 kb on + strand at 281.006 kb on - strand at 281.006 kb on - strand at 281.008 kb on - strand at 281.036 kb on + strand at 281.040 kb on + strand at 281.224 kb on + strand at 281.345 kb on + strand, within CA265_RS01110 at 281.400 kb on + strand, within CA265_RS01110 at 281.400 kb on + strand, within CA265_RS01110 at 281.401 kb on - strand, within CA265_RS01110 at 281.401 kb on - strand, within CA265_RS01110 at 281.439 kb on + strand, within CA265_RS01110 at 281.456 kb on + strand, within CA265_RS01110 at 281.457 kb on - strand, within CA265_RS01110 at 281.478 kb on + strand, within CA265_RS01110 at 281.648 kb on - strand, within CA265_RS01110 at 281.673 kb on - strand, within CA265_RS01110 at 281.794 kb on - strand, within CA265_RS01110 at 281.824 kb on - strand, within CA265_RS01110 at 281.883 kb on + strand, within CA265_RS01110 at 281.883 kb on + strand, within CA265_RS01110 at 281.883 kb on + strand, within CA265_RS01110 at 281.962 kb on + strand, within CA265_RS01110 at 281.963 kb on - strand, within CA265_RS01110 at 281.963 kb on - strand, within CA265_RS01110
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 278,136 - -2.5 278,162 - +1.6 278,177 + -0.5 278,210 + -0.1 278,211 - +0.9 278,211 - -0.8 278,211 - -0.3 278,222 + +0.2 278,234 + -2.2 278,234 + +0.6 278,294 - -0.4 278,326 - +0.6 278,376 + -0.0 278,391 + +0.5 278,391 + -0.4 278,392 - +0.6 278,468 - +2.2 278,497 + +0.0 278,640 - -1.0 278,660 - -0.8 278,740 + CA265_RS01100 0.23 +0.8 278,838 + CA265_RS01100 0.48 +0.8 278,838 + CA265_RS01100 0.48 +0.1 278,838 + CA265_RS01100 0.48 -0.4 278,839 - CA265_RS01100 0.48 -0.4 278,839 - CA265_RS01100 0.48 -0.6 278,871 - CA265_RS01100 0.56 +0.4 278,872 + CA265_RS01100 0.57 -0.2 278,873 - CA265_RS01100 0.57 -0.1 278,913 + CA265_RS01100 0.67 -1.5 278,926 - CA265_RS01100 0.71 -0.3 278,930 - CA265_RS01100 0.72 +0.6 278,936 + CA265_RS01100 0.73 +0.0 278,958 + CA265_RS01100 0.79 +0.1 279,012 + +0.5 279,013 - +1.1 279,039 - -0.1 279,192 + +1.0 279,260 + CA265_RS01105 0.10 +0.2 279,266 + CA265_RS01105 0.10 +0.0 279,267 - CA265_RS01105 0.10 +1.5 279,267 - CA265_RS01105 0.10 +0.6 279,422 - CA265_RS01105 0.19 +1.1 279,438 - CA265_RS01105 0.19 -0.3 279,485 + CA265_RS01105 0.22 -0.7 279,500 + CA265_RS01105 0.23 -0.6 279,500 + CA265_RS01105 0.23 +0.2 279,501 - CA265_RS01105 0.23 -1.0 279,501 - CA265_RS01105 0.23 -1.5 279,501 - CA265_RS01105 0.23 +0.3 279,503 - CA265_RS01105 0.23 +1.0 279,519 + CA265_RS01105 0.24 -0.3 279,520 - CA265_RS01105 0.24 +0.4 279,520 - CA265_RS01105 0.24 -0.1 279,585 - CA265_RS01105 0.27 +0.1 279,671 + CA265_RS01105 0.32 +0.2 279,752 - CA265_RS01105 0.36 -0.8 279,759 - CA265_RS01105 0.36 -0.0 279,767 + CA265_RS01105 0.37 -0.4 279,879 - CA265_RS01105 0.43 +0.8 279,946 + CA265_RS01105 0.46 +0.0 279,947 - CA265_RS01105 0.46 -1.3 279,947 - CA265_RS01105 0.46 -1.5 280,046 + CA265_RS01105 0.51 -1.3 280,047 - CA265_RS01105 0.51 +0.0 280,050 - CA265_RS01105 0.52 +1.4 280,092 + CA265_RS01105 0.54 -1.1 280,137 + CA265_RS01105 0.56 +0.0 280,161 + CA265_RS01105 0.57 -2.0 280,253 + CA265_RS01105 0.62 -0.6 280,341 + CA265_RS01105 0.67 -0.2 280,352 + CA265_RS01105 0.68 -1.0 280,383 + CA265_RS01105 0.69 -0.8 280,455 + CA265_RS01105 0.73 -0.7 280,478 + CA265_RS01105 0.74 -1.7 280,515 + CA265_RS01105 0.76 -0.4 280,587 + CA265_RS01105 0.80 +0.2 280,587 + CA265_RS01105 0.80 +0.3 280,588 - CA265_RS01105 0.80 -1.5 280,591 + CA265_RS01105 0.80 -0.1 280,591 + CA265_RS01105 0.80 +0.1 280,591 + CA265_RS01105 0.80 +0.1 280,591 + CA265_RS01105 0.80 +0.0 280,591 + CA265_RS01105 0.80 +0.1 280,592 - CA265_RS01105 0.80 -0.1 280,592 - CA265_RS01105 0.80 +0.2 280,592 - CA265_RS01105 0.80 +0.8 280,642 - CA265_RS01105 0.83 -1.0 280,681 + CA265_RS01105 0.85 -0.0 280,681 + CA265_RS01105 0.85 -0.2 280,719 + CA265_RS01105 0.87 -0.8 280,726 - CA265_RS01105 0.87 -0.8 280,739 + CA265_RS01105 0.88 -0.1 280,740 - CA265_RS01105 0.88 +0.3 280,803 + -0.5 280,807 - +0.8 280,828 + -0.1 280,937 - +0.0 280,966 + -1.2 281,005 + +0.6 281,006 - -0.5 281,006 - +0.1 281,008 - +1.0 281,036 + +0.4 281,040 + +0.0 281,224 + +0.9 281,345 + CA265_RS01110 0.14 -0.9 281,400 + CA265_RS01110 0.16 +0.6 281,400 + CA265_RS01110 0.16 +1.4 281,401 - CA265_RS01110 0.16 -0.0 281,401 - CA265_RS01110 0.16 -1.9 281,439 + CA265_RS01110 0.18 -0.4 281,456 + CA265_RS01110 0.19 +1.0 281,457 - CA265_RS01110 0.19 +0.1 281,478 + CA265_RS01110 0.20 +0.8 281,648 - CA265_RS01110 0.27 +2.0 281,673 - CA265_RS01110 0.28 +0.2 281,794 - CA265_RS01110 0.32 -0.0 281,824 - CA265_RS01110 0.34 +0.5 281,883 + CA265_RS01110 0.36 -0.6 281,883 + CA265_RS01110 0.36 -0.5 281,883 + CA265_RS01110 0.36 +0.8 281,962 + CA265_RS01110 0.39 -1.1 281,963 - CA265_RS01110 0.39 +1.1 281,963 - CA265_RS01110 0.39 +0.6
Or see this region's nucleotide sequence