Experiment: Formate pooled triplicate biofilm cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01195 and MMJJ_RS01200 are separated by 24 nucleotides MMJJ_RS01200 and MMJJ_RS09335 are separated by 125 nucleotides MMJJ_RS09335 and MMJJ_RS01205 are separated by 28 nucleotides MMJJ_RS01205 and MMJJ_RS01210 are separated by 74 nucleotides MMJJ_RS01210 and MMJJ_RS01215 are separated by 135 nucleotides
MMJJ_RS01195: MMJJ_RS01195 - aspartate dehydrogenase, at 229,929 to 230,732
_RS01195
MMJJ_RS01200: MMJJ_RS01200 - PRC-barrel domain-containing protein, at 230,757 to 231,011
_RS01200
MMJJ_RS09335: MMJJ_RS09335 - hypothetical protein, at 231,137 to 231,307
_RS09335
MMJJ_RS01205: MMJJ_RS01205 - DNA adenine methylase, at 231,336 to 232,256
_RS01205
MMJJ_RS01210: MMJJ_RS01210 - hypothetical protein, at 232,331 to 232,648
_RS01210
MMJJ_RS01215: MMJJ_RS01215 - MATE family efflux transporter, at 232,784 to 234,142
_RS01215
Position (kb) 231
232
233 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 230.736 kb on + strand at 230.771 kb on - strand at 230.953 kb on - strand at 230.953 kb on - strand, within MMJJ_RS01200 at 230.953 kb on - strand, within MMJJ_RS01200 at 230.953 kb on - strand, within MMJJ_RS01200 at 230.954 kb on + strand, within MMJJ_RS01200 at 230.954 kb on + strand, within MMJJ_RS01200 at 230.954 kb on + strand, within MMJJ_RS01200 at 230.956 kb on + strand, within MMJJ_RS01200 at 230.957 kb on - strand, within MMJJ_RS01200 at 230.957 kb on - strand, within MMJJ_RS01200 at 231.014 kb on - strand at 231.043 kb on + strand at 231.054 kb on + strand at 231.064 kb on + strand at 231.086 kb on - strand at 231.087 kb on + strand at 231.090 kb on + strand at 231.092 kb on + strand at 231.092 kb on + strand at 231.092 kb on + strand at 231.101 kb on + strand at 231.101 kb on + strand at 231.102 kb on - strand at 231.103 kb on + strand at 231.131 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.136 kb on - strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.137 kb on + strand at 231.150 kb on + strand at 231.150 kb on + strand at 231.150 kb on + strand at 231.181 kb on - strand, within MMJJ_RS09335 at 231.181 kb on - strand, within MMJJ_RS09335 at 231.181 kb on - strand, within MMJJ_RS09335 at 231.181 kb on - strand, within MMJJ_RS09335 at 231.182 kb on + strand, within MMJJ_RS09335 at 231.182 kb on + strand, within MMJJ_RS09335 at 231.182 kb on + strand, within MMJJ_RS09335 at 231.184 kb on + strand, within MMJJ_RS09335 at 231.184 kb on + strand, within MMJJ_RS09335 at 231.263 kb on + strand, within MMJJ_RS09335 at 231.263 kb on + strand at 231.268 kb on + strand, within MMJJ_RS09335 at 231.306 kb on + strand at 231.317 kb on - strand at 231.318 kb on + strand at 231.320 kb on - strand at 231.330 kb on - strand at 231.402 kb on - strand at 231.431 kb on - strand, within MMJJ_RS01205 at 231.432 kb on + strand, within MMJJ_RS01205 at 231.442 kb on - strand, within MMJJ_RS01205 at 231.476 kb on - strand, within MMJJ_RS01205 at 231.476 kb on - strand, within MMJJ_RS01205 at 231.476 kb on - strand, within MMJJ_RS01205 at 231.477 kb on + strand, within MMJJ_RS01205 at 231.494 kb on - strand, within MMJJ_RS01205 at 231.505 kb on + strand, within MMJJ_RS01205 at 231.505 kb on + strand, within MMJJ_RS01205 at 231.508 kb on - strand at 231.508 kb on - strand, within MMJJ_RS01205 at 231.508 kb on - strand, within MMJJ_RS01205 at 231.509 kb on + strand, within MMJJ_RS01205 at 231.509 kb on + strand, within MMJJ_RS01205 at 231.509 kb on + strand, within MMJJ_RS01205 at 231.509 kb on + strand, within MMJJ_RS01205 at 231.509 kb on + strand, within MMJJ_RS01205 at 231.534 kb on + strand, within MMJJ_RS01205 at 231.582 kb on + strand, within MMJJ_RS01205 at 231.582 kb on + strand, within MMJJ_RS01205 at 231.594 kb on + strand, within MMJJ_RS01205 at 231.599 kb on - strand, within MMJJ_RS01205 at 231.637 kb on - strand, within MMJJ_RS01205 at 231.638 kb on + strand, within MMJJ_RS01205 at 231.638 kb on + strand, within MMJJ_RS01205 at 231.639 kb on - strand, within MMJJ_RS01205 at 231.683 kb on - strand, within MMJJ_RS01205 at 231.685 kb on - strand, within MMJJ_RS01205 at 231.769 kb on - strand, within MMJJ_RS01205 at 231.770 kb on + strand, within MMJJ_RS01205 at 231.803 kb on - strand, within MMJJ_RS01205 at 231.813 kb on + strand, within MMJJ_RS01205 at 231.825 kb on + strand, within MMJJ_RS01205 at 231.891 kb on + strand, within MMJJ_RS01205 at 231.891 kb on + strand, within MMJJ_RS01205 at 231.916 kb on - strand, within MMJJ_RS01205 at 231.917 kb on + strand, within MMJJ_RS01205 at 231.927 kb on - strand, within MMJJ_RS01205 at 231.935 kb on + strand, within MMJJ_RS01205 at 231.937 kb on - strand, within MMJJ_RS01205 at 231.937 kb on - strand, within MMJJ_RS01205 at 231.938 kb on + strand, within MMJJ_RS01205 at 231.986 kb on - strand, within MMJJ_RS01205 at 232.006 kb on + strand, within MMJJ_RS01205 at 232.019 kb on - strand, within MMJJ_RS01205 at 232.034 kb on - strand, within MMJJ_RS01205 at 232.090 kb on - strand, within MMJJ_RS01205 at 232.275 kb on + strand at 232.285 kb on - strand at 232.285 kb on - strand at 232.286 kb on + strand at 232.287 kb on - strand at 232.287 kb on - strand at 232.287 kb on - strand at 232.287 kb on - strand at 232.287 kb on - strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.288 kb on + strand at 232.291 kb on - strand at 232.292 kb on + strand at 232.293 kb on - strand at 232.347 kb on + strand at 232.352 kb on - strand at 232.352 kb on - strand at 232.353 kb on + strand at 232.353 kb on + strand at 232.353 kb on + strand at 232.404 kb on + strand, within MMJJ_RS01210 at 232.404 kb on + strand, within MMJJ_RS01210 at 232.478 kb on - strand, within MMJJ_RS01210 at 232.479 kb on + strand, within MMJJ_RS01210 at 232.648 kb on - strand at 232.648 kb on - strand at 232.649 kb on + strand at 232.650 kb on - strand at 232.650 kb on - strand at 232.650 kb on - strand at 232.651 kb on + strand at 232.664 kb on - strand at 232.665 kb on + strand at 232.667 kb on + strand at 232.667 kb on + strand at 232.667 kb on + strand at 232.683 kb on + strand at 232.701 kb on - strand at 232.701 kb on - strand at 232.701 kb on - strand at 232.701 kb on - strand at 232.701 kb on - strand at 232.701 kb on - strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.702 kb on + strand at 232.703 kb on - strand at 232.703 kb on - strand at 232.703 kb on - strand at 232.703 kb on - strand at 232.703 kb on - strand at 232.703 kb on - strand at 232.704 kb on + strand at 232.704 kb on + strand at 232.704 kb on + strand at 232.705 kb on - strand at 232.705 kb on - strand at 232.705 kb on - strand at 232.705 kb on - strand at 232.706 kb on + strand at 232.706 kb on + strand at 232.706 kb on + strand at 232.706 kb on + strand at 232.712 kb on - strand at 232.722 kb on - strand at 232.724 kb on - strand at 232.725 kb on + strand at 232.823 kb on + strand at 232.833 kb on - strand at 232.833 kb on - strand at 232.834 kb on + strand at 232.834 kb on + strand at 232.838 kb on - strand at 232.838 kb on - strand at 232.838 kb on - strand at 232.838 kb on - strand at 232.838 kb on - strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.839 kb on + strand at 232.871 kb on + strand at 232.899 kb on - strand at 232.972 kb on - strand, within MMJJ_RS01215 at 232.973 kb on + strand, within MMJJ_RS01215 at 232.973 kb on + strand, within MMJJ_RS01215 at 233.013 kb on - strand, within MMJJ_RS01215 at 233.065 kb on + strand, within MMJJ_RS01215 at 233.065 kb on + strand, within MMJJ_RS01215 at 233.081 kb on - strand, within MMJJ_RS01215 at 233.081 kb on - strand, within MMJJ_RS01215 at 233.082 kb on + strand, within MMJJ_RS01215 at 233.082 kb on + strand, within MMJJ_RS01215 at 233.082 kb on + strand, within MMJJ_RS01215 at 233.082 kb on + strand, within MMJJ_RS01215 at 233.082 kb on + strand, within MMJJ_RS01215 at 233.086 kb on - strand, within MMJJ_RS01215 at 233.088 kb on - strand, within MMJJ_RS01215 at 233.107 kb on - strand, within MMJJ_RS01215 at 233.108 kb on + strand, within MMJJ_RS01215 at 233.127 kb on - strand, within MMJJ_RS01215 at 233.128 kb on + strand, within MMJJ_RS01215 at 233.130 kb on - strand, within MMJJ_RS01215 at 233.131 kb on + strand, within MMJJ_RS01215 at 233.175 kb on - strand, within MMJJ_RS01215 at 233.175 kb on - strand, within MMJJ_RS01215 at 233.176 kb on + strand, within MMJJ_RS01215 at 233.176 kb on + strand, within MMJJ_RS01215 at 233.176 kb on + strand, within MMJJ_RS01215 at 233.176 kb on + strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.177 kb on - strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.178 kb on + strand, within MMJJ_RS01215 at 233.202 kb on - strand, within MMJJ_RS01215 at 233.254 kb on - strand, within MMJJ_RS01215 at 233.254 kb on - strand, within MMJJ_RS01215 at 233.254 kb on - strand, within MMJJ_RS01215
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate biofilm cells remove 230,736 + -0.8 230,771 - -2.8 230,953 - -0.8 230,953 - MMJJ_RS01200 0.77 -2.4 230,953 - MMJJ_RS01200 0.77 -2.1 230,953 - MMJJ_RS01200 0.77 -1.4 230,954 + MMJJ_RS01200 0.77 -2.4 230,954 + MMJJ_RS01200 0.77 -1.4 230,954 + MMJJ_RS01200 0.77 -0.8 230,956 + MMJJ_RS01200 0.78 -2.1 230,957 - MMJJ_RS01200 0.78 -2.4 230,957 - MMJJ_RS01200 0.78 -2.4 231,014 - -3.0 231,043 + -1.4 231,054 + -2.1 231,064 + -3.6 231,086 - +0.3 231,087 + +0.9 231,090 + -0.2 231,092 + -0.9 231,092 + +0.2 231,092 + +0.1 231,101 + +2.6 231,101 + -0.3 231,102 - -0.2 231,103 + -0.5 231,131 - -0.8 231,136 - -1.1 231,136 - -0.1 231,136 - +1.0 231,136 - +1.2 231,136 - +0.3 231,136 - -0.5 231,136 - -0.0 231,136 - +0.4 231,136 - -1.2 231,137 + +0.4 231,137 + -0.5 231,137 + -0.7 231,137 + +0.1 231,137 + +0.7 231,137 + -0.2 231,137 + +0.1 231,137 + +0.4 231,137 + -0.0 231,150 + -0.0 231,150 + -0.1 231,150 + -0.1 231,181 - MMJJ_RS09335 0.26 -1.0 231,181 - MMJJ_RS09335 0.26 +3.0 231,181 - MMJJ_RS09335 0.26 +0.5 231,181 - MMJJ_RS09335 0.26 -0.4 231,182 + MMJJ_RS09335 0.26 +0.2 231,182 + MMJJ_RS09335 0.26 +1.2 231,182 + MMJJ_RS09335 0.26 -0.3 231,184 + MMJJ_RS09335 0.27 +0.2 231,184 + MMJJ_RS09335 0.27 -1.0 231,263 + MMJJ_RS09335 0.74 +0.0 231,263 + -1.4 231,268 + MMJJ_RS09335 0.77 -1.1 231,306 + -0.6 231,317 - -0.1 231,318 + -0.6 231,320 - -0.4 231,330 - -0.5 231,402 - +0.0 231,431 - MMJJ_RS01205 0.10 -1.8 231,432 + MMJJ_RS01205 0.10 -0.8 231,442 - MMJJ_RS01205 0.12 -0.4 231,476 - MMJJ_RS01205 0.15 +0.8 231,476 - MMJJ_RS01205 0.15 +1.5 231,476 - MMJJ_RS01205 0.15 -0.3 231,477 + MMJJ_RS01205 0.15 +0.7 231,494 - MMJJ_RS01205 0.17 +0.9 231,505 + MMJJ_RS01205 0.18 -0.4 231,505 + MMJJ_RS01205 0.18 -0.2 231,508 - -0.8 231,508 - MMJJ_RS01205 0.19 +0.1 231,508 - MMJJ_RS01205 0.19 -0.1 231,509 + MMJJ_RS01205 0.19 -0.3 231,509 + MMJJ_RS01205 0.19 +0.3 231,509 + MMJJ_RS01205 0.19 +0.5 231,509 + MMJJ_RS01205 0.19 -0.6 231,509 + MMJJ_RS01205 0.19 -0.1 231,534 + MMJJ_RS01205 0.21 -0.2 231,582 + MMJJ_RS01205 0.27 +0.8 231,582 + MMJJ_RS01205 0.27 +1.0 231,594 + MMJJ_RS01205 0.28 -0.3 231,599 - MMJJ_RS01205 0.29 +0.1 231,637 - MMJJ_RS01205 0.33 -0.1 231,638 + MMJJ_RS01205 0.33 -0.5 231,638 + MMJJ_RS01205 0.33 -0.3 231,639 - MMJJ_RS01205 0.33 +0.3 231,683 - MMJJ_RS01205 0.38 -0.7 231,685 - MMJJ_RS01205 0.38 -0.8 231,769 - MMJJ_RS01205 0.47 +0.2 231,770 + MMJJ_RS01205 0.47 +0.2 231,803 - MMJJ_RS01205 0.51 +0.3 231,813 + MMJJ_RS01205 0.52 +0.1 231,825 + MMJJ_RS01205 0.53 -0.5 231,891 + MMJJ_RS01205 0.60 -0.3 231,891 + MMJJ_RS01205 0.60 -0.6 231,916 - MMJJ_RS01205 0.63 +0.5 231,917 + MMJJ_RS01205 0.63 -1.2 231,927 - MMJJ_RS01205 0.64 -0.4 231,935 + MMJJ_RS01205 0.65 -0.3 231,937 - MMJJ_RS01205 0.65 -0.6 231,937 - MMJJ_RS01205 0.65 -0.3 231,938 + MMJJ_RS01205 0.65 +0.4 231,986 - MMJJ_RS01205 0.71 +0.8 232,006 + MMJJ_RS01205 0.73 -0.4 232,019 - MMJJ_RS01205 0.74 +1.5 232,034 - MMJJ_RS01205 0.76 -0.1 232,090 - MMJJ_RS01205 0.82 -0.2 232,275 + -0.3 232,285 - -0.3 232,285 - +0.4 232,286 + +0.6 232,287 - +0.6 232,287 - +0.4 232,287 - +3.5 232,287 - -0.2 232,287 - +0.3 232,288 + -0.7 232,288 + +1.2 232,288 + -0.1 232,288 + +0.7 232,288 + -0.3 232,288 + -0.2 232,288 + +0.9 232,291 - +3.6 232,292 + +0.2 232,293 - +0.2 232,347 + +0.2 232,352 - -0.3 232,352 - -0.1 232,353 + +0.9 232,353 + -0.1 232,353 + +0.2 232,404 + MMJJ_RS01210 0.23 +3.6 232,404 + MMJJ_RS01210 0.23 +2.7 232,478 - MMJJ_RS01210 0.46 -0.0 232,479 + MMJJ_RS01210 0.47 -0.3 232,648 - -0.8 232,648 - +0.2 232,649 + -0.2 232,650 - -0.2 232,650 - +0.6 232,650 - +0.2 232,651 + +0.7 232,664 - -0.6 232,665 + +0.7 232,667 + +0.7 232,667 + -0.1 232,667 + -0.3 232,683 + -0.9 232,701 - +2.0 232,701 - +1.2 232,701 - -0.0 232,701 - +0.7 232,701 - -0.1 232,701 - -0.2 232,702 + +0.1 232,702 + -0.1 232,702 + -0.0 232,702 + +0.1 232,702 + +0.0 232,702 + -1.0 232,702 + +0.7 232,702 + +0.5 232,702 + -0.1 232,702 + +0.1 232,703 - +0.1 232,703 - +0.6 232,703 - -0.1 232,703 - +0.3 232,703 - -0.6 232,703 - +0.3 232,704 + -0.2 232,704 + -0.7 232,704 + -0.6 232,705 - +0.3 232,705 - +0.1 232,705 - -0.9 232,705 - +0.2 232,706 + +0.9 232,706 + +0.3 232,706 + -0.1 232,706 + +0.2 232,712 - +2.5 232,722 - -0.0 232,724 - -0.6 232,725 + -0.0 232,823 + -0.4 232,833 - -0.1 232,833 - -0.8 232,834 + +0.6 232,834 + +0.3 232,838 - -0.1 232,838 - -0.1 232,838 - -0.3 232,838 - -0.8 232,838 - -0.4 232,839 + -0.9 232,839 + -0.1 232,839 + -0.8 232,839 + -0.3 232,839 + +2.5 232,839 + -2.4 232,839 + -0.2 232,871 + +0.1 232,899 - +0.1 232,972 - MMJJ_RS01215 0.14 +0.0 232,973 + MMJJ_RS01215 0.14 +3.0 232,973 + MMJJ_RS01215 0.14 -0.5 233,013 - MMJJ_RS01215 0.17 -0.1 233,065 + MMJJ_RS01215 0.21 +0.0 233,065 + MMJJ_RS01215 0.21 -0.1 233,081 - MMJJ_RS01215 0.22 +0.2 233,081 - MMJJ_RS01215 0.22 -0.2 233,082 + MMJJ_RS01215 0.22 -1.3 233,082 + MMJJ_RS01215 0.22 +0.1 233,082 + MMJJ_RS01215 0.22 +0.5 233,082 + MMJJ_RS01215 0.22 +0.3 233,082 + MMJJ_RS01215 0.22 +0.0 233,086 - MMJJ_RS01215 0.22 +0.1 233,088 - MMJJ_RS01215 0.22 +3.3 233,107 - MMJJ_RS01215 0.24 -0.4 233,108 + MMJJ_RS01215 0.24 +0.3 233,127 - MMJJ_RS01215 0.25 +1.9 233,128 + MMJJ_RS01215 0.25 +0.0 233,130 - MMJJ_RS01215 0.25 +3.4 233,131 + MMJJ_RS01215 0.26 -0.1 233,175 - MMJJ_RS01215 0.29 -0.3 233,175 - MMJJ_RS01215 0.29 +0.1 233,176 + MMJJ_RS01215 0.29 +0.3 233,176 + MMJJ_RS01215 0.29 +0.2 233,176 + MMJJ_RS01215 0.29 -0.1 233,176 + MMJJ_RS01215 0.29 -0.3 233,177 - MMJJ_RS01215 0.29 +0.2 233,177 - MMJJ_RS01215 0.29 -0.1 233,177 - MMJJ_RS01215 0.29 -0.1 233,177 - MMJJ_RS01215 0.29 +0.1 233,177 - MMJJ_RS01215 0.29 -0.8 233,177 - MMJJ_RS01215 0.29 -0.3 233,177 - MMJJ_RS01215 0.29 +0.1 233,178 + MMJJ_RS01215 0.29 +0.2 233,178 + MMJJ_RS01215 0.29 +0.4 233,178 + MMJJ_RS01215 0.29 -0.6 233,178 + MMJJ_RS01215 0.29 +0.1 233,178 + MMJJ_RS01215 0.29 -1.3 233,178 + MMJJ_RS01215 0.29 +0.6 233,178 + MMJJ_RS01215 0.29 +0.2 233,202 - MMJJ_RS01215 0.31 -0.3 233,254 - MMJJ_RS01215 0.35 -0.3 233,254 - MMJJ_RS01215 0.35 -0.4 233,254 - MMJJ_RS01215 0.35 -0.0
Or see this region's nucleotide sequence