Experiment: Formate pooled triplicate biofilm cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00860 and MMJJ_RS00865 are separated by 8 nucleotides MMJJ_RS00865 and MMJJ_RS00870 are separated by 21 nucleotides MMJJ_RS00870 and MMJJ_RS00875 are separated by 16 nucleotides
MMJJ_RS00860: MMJJ_RS00860 - 4Fe-4S binding protein, at 164,474 to 165,667
_RS00860
MMJJ_RS00865: MMJJ_RS00865 - Ni/Fe hydrogenase subunit alpha, at 165,676 to 167,091
_RS00865
MMJJ_RS00870: MMJJ_RS00870 - F420-nonreducing hydrogenase, at 167,113 to 168,015
_RS00870
MMJJ_RS00875: MMJJ_RS00875 - hydrogenase iron-sulfur subunit, at 168,032 to 168,454
_RS00875
Position (kb)
165
166
167
168 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 164.738 kb on + strand, within MMJJ_RS00860 at 164.835 kb on + strand, within MMJJ_RS00860 at 164.853 kb on - strand, within MMJJ_RS00860 at 164.960 kb on - strand, within MMJJ_RS00860 at 164.960 kb on - strand, within MMJJ_RS00860 at 164.961 kb on + strand, within MMJJ_RS00860 at 165.008 kb on - strand, within MMJJ_RS00860 at 165.018 kb on + strand, within MMJJ_RS00860 at 165.062 kb on - strand, within MMJJ_RS00860 at 165.062 kb on - strand, within MMJJ_RS00860 at 165.063 kb on + strand, within MMJJ_RS00860 at 165.063 kb on + strand, within MMJJ_RS00860 at 165.092 kb on + strand, within MMJJ_RS00860 at 165.166 kb on - strand, within MMJJ_RS00860 at 165.171 kb on - strand, within MMJJ_RS00860 at 165.172 kb on + strand, within MMJJ_RS00860 at 165.172 kb on + strand, within MMJJ_RS00860 at 165.174 kb on + strand, within MMJJ_RS00860 at 165.174 kb on + strand, within MMJJ_RS00860 at 165.176 kb on + strand, within MMJJ_RS00860 at 165.244 kb on - strand, within MMJJ_RS00860 at 165.244 kb on - strand, within MMJJ_RS00860 at 165.245 kb on + strand, within MMJJ_RS00860 at 165.245 kb on + strand, within MMJJ_RS00860 at 165.248 kb on - strand, within MMJJ_RS00860 at 165.301 kb on - strand, within MMJJ_RS00860 at 165.326 kb on - strand, within MMJJ_RS00860 at 165.326 kb on - strand, within MMJJ_RS00860 at 165.345 kb on - strand, within MMJJ_RS00860 at 165.490 kb on - strand, within MMJJ_RS00860 at 165.494 kb on + strand, within MMJJ_RS00860 at 165.506 kb on - strand, within MMJJ_RS00860 at 165.522 kb on + strand, within MMJJ_RS00860 at 165.637 kb on - strand at 165.640 kb on + strand at 165.640 kb on + strand at 165.787 kb on - strand at 165.805 kb on - strand at 165.806 kb on + strand at 165.838 kb on - strand, within MMJJ_RS00865 at 165.839 kb on + strand, within MMJJ_RS00865 at 165.839 kb on + strand, within MMJJ_RS00865 at 165.907 kb on - strand, within MMJJ_RS00865 at 165.908 kb on + strand, within MMJJ_RS00865 at 166.018 kb on - strand, within MMJJ_RS00865 at 166.019 kb on + strand, within MMJJ_RS00865 at 166.025 kb on + strand, within MMJJ_RS00865 at 166.066 kb on - strand, within MMJJ_RS00865 at 166.106 kb on - strand, within MMJJ_RS00865 at 166.167 kb on + strand, within MMJJ_RS00865 at 166.179 kb on - strand, within MMJJ_RS00865 at 166.180 kb on + strand, within MMJJ_RS00865 at 166.180 kb on + strand, within MMJJ_RS00865 at 166.185 kb on - strand, within MMJJ_RS00865 at 166.185 kb on - strand, within MMJJ_RS00865 at 166.201 kb on - strand, within MMJJ_RS00865 at 166.201 kb on - strand, within MMJJ_RS00865 at 166.201 kb on - strand, within MMJJ_RS00865 at 166.201 kb on - strand, within MMJJ_RS00865 at 166.201 kb on - strand, within MMJJ_RS00865 at 166.202 kb on + strand, within MMJJ_RS00865 at 166.202 kb on + strand, within MMJJ_RS00865 at 166.310 kb on - strand, within MMJJ_RS00865 at 166.315 kb on - strand, within MMJJ_RS00865 at 166.315 kb on - strand, within MMJJ_RS00865 at 166.316 kb on + strand, within MMJJ_RS00865 at 166.319 kb on - strand, within MMJJ_RS00865 at 166.385 kb on - strand, within MMJJ_RS00865 at 166.386 kb on + strand, within MMJJ_RS00865 at 166.389 kb on - strand, within MMJJ_RS00865 at 166.437 kb on + strand, within MMJJ_RS00865 at 166.477 kb on - strand, within MMJJ_RS00865 at 166.492 kb on - strand, within MMJJ_RS00865 at 166.516 kb on - strand, within MMJJ_RS00865 at 166.517 kb on + strand, within MMJJ_RS00865 at 166.517 kb on + strand, within MMJJ_RS00865 at 166.597 kb on - strand, within MMJJ_RS00865 at 166.608 kb on - strand, within MMJJ_RS00865 at 166.609 kb on + strand, within MMJJ_RS00865 at 166.672 kb on - strand, within MMJJ_RS00865 at 166.673 kb on + strand, within MMJJ_RS00865 at 166.744 kb on - strand, within MMJJ_RS00865 at 167.092 kb on - strand at 167.102 kb on - strand at 167.293 kb on + strand, within MMJJ_RS00870 at 167.311 kb on + strand, within MMJJ_RS00870 at 167.335 kb on - strand, within MMJJ_RS00870 at 167.496 kb on - strand, within MMJJ_RS00870 at 167.535 kb on - strand, within MMJJ_RS00870 at 167.535 kb on - strand, within MMJJ_RS00870 at 167.536 kb on + strand, within MMJJ_RS00870 at 167.536 kb on + strand, within MMJJ_RS00870 at 167.540 kb on + strand, within MMJJ_RS00870 at 167.540 kb on + strand, within MMJJ_RS00870 at 167.540 kb on + strand, within MMJJ_RS00870 at 167.583 kb on - strand, within MMJJ_RS00870 at 167.583 kb on - strand, within MMJJ_RS00870 at 167.655 kb on - strand, within MMJJ_RS00870 at 167.693 kb on - strand, within MMJJ_RS00870 at 167.725 kb on - strand, within MMJJ_RS00870 at 167.726 kb on + strand, within MMJJ_RS00870 at 167.731 kb on + strand, within MMJJ_RS00870 at 167.866 kb on + strand, within MMJJ_RS00870 at 167.950 kb on - strand at 167.950 kb on - strand at 167.965 kb on - strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.010 kb on - strand at 168.015 kb on - strand at 168.015 kb on - strand at 168.029 kb on - strand at 168.029 kb on - strand at 168.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate biofilm cells remove 164,738 + MMJJ_RS00860 0.22 +0.9 164,835 + MMJJ_RS00860 0.30 -0.0 164,853 - MMJJ_RS00860 0.32 -1.1 164,960 - MMJJ_RS00860 0.41 -1.0 164,960 - MMJJ_RS00860 0.41 +0.9 164,961 + MMJJ_RS00860 0.41 -0.2 165,008 - MMJJ_RS00860 0.45 -0.5 165,018 + MMJJ_RS00860 0.46 -0.3 165,062 - MMJJ_RS00860 0.49 -0.1 165,062 - MMJJ_RS00860 0.49 +0.2 165,063 + MMJJ_RS00860 0.49 +0.3 165,063 + MMJJ_RS00860 0.49 +0.4 165,092 + MMJJ_RS00860 0.52 +0.8 165,166 - MMJJ_RS00860 0.58 +2.9 165,171 - MMJJ_RS00860 0.58 -0.3 165,172 + MMJJ_RS00860 0.58 -0.0 165,172 + MMJJ_RS00860 0.58 +0.1 165,174 + MMJJ_RS00860 0.59 -0.8 165,174 + MMJJ_RS00860 0.59 -0.6 165,176 + MMJJ_RS00860 0.59 +0.4 165,244 - MMJJ_RS00860 0.64 -0.6 165,244 - MMJJ_RS00860 0.64 -0.1 165,245 + MMJJ_RS00860 0.65 -0.3 165,245 + MMJJ_RS00860 0.65 +2.0 165,248 - MMJJ_RS00860 0.65 +0.2 165,301 - MMJJ_RS00860 0.69 +2.4 165,326 - MMJJ_RS00860 0.71 +0.1 165,326 - MMJJ_RS00860 0.71 -0.3 165,345 - MMJJ_RS00860 0.73 -0.8 165,490 - MMJJ_RS00860 0.85 +0.2 165,494 + MMJJ_RS00860 0.85 +0.1 165,506 - MMJJ_RS00860 0.86 -1.2 165,522 + MMJJ_RS00860 0.88 +0.2 165,637 - -0.7 165,640 + +1.2 165,640 + -0.2 165,787 - +0.3 165,805 - -0.1 165,806 + +0.8 165,838 - MMJJ_RS00865 0.11 -0.2 165,839 + MMJJ_RS00865 0.12 -0.4 165,839 + MMJJ_RS00865 0.12 +1.2 165,907 - MMJJ_RS00865 0.16 +0.4 165,908 + MMJJ_RS00865 0.16 +3.1 166,018 - MMJJ_RS00865 0.24 -0.1 166,019 + MMJJ_RS00865 0.24 +2.6 166,025 + MMJJ_RS00865 0.25 +2.2 166,066 - MMJJ_RS00865 0.28 +0.5 166,106 - MMJJ_RS00865 0.30 -0.8 166,167 + MMJJ_RS00865 0.35 -0.3 166,179 - MMJJ_RS00865 0.36 -0.1 166,180 + MMJJ_RS00865 0.36 -0.3 166,180 + MMJJ_RS00865 0.36 -1.7 166,185 - MMJJ_RS00865 0.36 +0.1 166,185 - MMJJ_RS00865 0.36 +0.1 166,201 - MMJJ_RS00865 0.37 +3.3 166,201 - MMJJ_RS00865 0.37 -0.0 166,201 - MMJJ_RS00865 0.37 -0.3 166,201 - MMJJ_RS00865 0.37 +1.0 166,201 - MMJJ_RS00865 0.37 +0.4 166,202 + MMJJ_RS00865 0.37 -1.4 166,202 + MMJJ_RS00865 0.37 +1.2 166,310 - MMJJ_RS00865 0.45 +3.3 166,315 - MMJJ_RS00865 0.45 +0.1 166,315 - MMJJ_RS00865 0.45 +0.2 166,316 + MMJJ_RS00865 0.45 +0.5 166,319 - MMJJ_RS00865 0.45 -0.3 166,385 - MMJJ_RS00865 0.50 -0.0 166,386 + MMJJ_RS00865 0.50 +0.1 166,389 - MMJJ_RS00865 0.50 -0.4 166,437 + MMJJ_RS00865 0.54 -0.2 166,477 - MMJJ_RS00865 0.57 -0.2 166,492 - MMJJ_RS00865 0.58 +2.7 166,516 - MMJJ_RS00865 0.59 -0.2 166,517 + MMJJ_RS00865 0.59 -0.1 166,517 + MMJJ_RS00865 0.59 +0.3 166,597 - MMJJ_RS00865 0.65 +0.2 166,608 - MMJJ_RS00865 0.66 +0.6 166,609 + MMJJ_RS00865 0.66 +0.2 166,672 - MMJJ_RS00865 0.70 +0.1 166,673 + MMJJ_RS00865 0.70 +0.2 166,744 - MMJJ_RS00865 0.75 +0.3 167,092 - +0.2 167,102 - -0.3 167,293 + MMJJ_RS00870 0.20 -1.7 167,311 + MMJJ_RS00870 0.22 +0.3 167,335 - MMJJ_RS00870 0.25 -1.3 167,496 - MMJJ_RS00870 0.42 -0.0 167,535 - MMJJ_RS00870 0.47 -0.8 167,535 - MMJJ_RS00870 0.47 +0.8 167,536 + MMJJ_RS00870 0.47 -1.0 167,536 + MMJJ_RS00870 0.47 +0.1 167,540 + MMJJ_RS00870 0.47 -0.3 167,540 + MMJJ_RS00870 0.47 -0.1 167,540 + MMJJ_RS00870 0.47 -0.1 167,583 - MMJJ_RS00870 0.52 -0.0 167,583 - MMJJ_RS00870 0.52 -0.2 167,655 - MMJJ_RS00870 0.60 -0.1 167,693 - MMJJ_RS00870 0.64 +1.1 167,725 - MMJJ_RS00870 0.68 +0.2 167,726 + MMJJ_RS00870 0.68 +0.2 167,731 + MMJJ_RS00870 0.68 +1.0 167,866 + MMJJ_RS00870 0.83 +0.2 167,950 - -2.1 167,950 - -0.1 167,965 - +3.4 168,001 + -1.4 168,001 + -0.5 168,001 + +0.2 168,001 + +0.8 168,010 - -0.3 168,015 - -0.2 168,015 - -1.4 168,029 - +0.2 168,029 - +0.2 168,030 + +0.1
Or see this region's nucleotide sequence