Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09230 and EX31_RS09235 are separated by 7 nucleotides EX31_RS09235 and EX31_RS09240 are separated by 90 nucleotides
EX31_RS09230: EX31_RS09230 - LysR family transcriptional regulator, at 44,316 to 45,215
_RS09230
EX31_RS09235: EX31_RS09235 - M55 family metallopeptidase, at 45,223 to 46,044
_RS09235
EX31_RS09240: EX31_RS09240 - D-aminopeptidase, at 46,135 to 47,199
_RS09240
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 44.480 kb on - strand, within EX31_RS09230 at 44.551 kb on + strand, within EX31_RS09230 at 44.723 kb on + strand, within EX31_RS09230 at 44.962 kb on - strand, within EX31_RS09230 at 44.962 kb on - strand, within EX31_RS09230 at 44.962 kb on - strand, within EX31_RS09230 at 44.994 kb on - strand, within EX31_RS09230 at 44.994 kb on - strand, within EX31_RS09230 at 45.066 kb on - strand, within EX31_RS09230 at 45.177 kb on + strand at 45.207 kb on + strand at 45.225 kb on - strand at 45.271 kb on - strand at 45.274 kb on - strand at 45.274 kb on - strand at 45.530 kb on + strand, within EX31_RS09235 at 45.530 kb on + strand, within EX31_RS09235 at 45.530 kb on + strand, within EX31_RS09235 at 45.531 kb on - strand, within EX31_RS09235 at 45.546 kb on - strand, within EX31_RS09235 at 45.631 kb on + strand, within EX31_RS09235 at 45.631 kb on + strand, within EX31_RS09235 at 45.631 kb on + strand, within EX31_RS09235 at 45.631 kb on + strand, within EX31_RS09235 at 45.632 kb on - strand, within EX31_RS09235 at 45.632 kb on - strand, within EX31_RS09235 at 45.759 kb on - strand, within EX31_RS09235 at 46.084 kb on - strand at 46.137 kb on - strand at 46.150 kb on - strand at 46.153 kb on + strand at 46.154 kb on - strand at 46.154 kb on - strand at 46.227 kb on + strand at 46.228 kb on - strand at 46.443 kb on + strand, within EX31_RS09240 at 46.565 kb on - strand, within EX31_RS09240 at 46.571 kb on - strand, within EX31_RS09240 at 46.624 kb on + strand, within EX31_RS09240 at 46.625 kb on - strand, within EX31_RS09240 at 46.744 kb on - strand, within EX31_RS09240 at 46.744 kb on - strand, within EX31_RS09240 at 46.748 kb on + strand, within EX31_RS09240 at 46.748 kb on + strand, within EX31_RS09240 at 46.749 kb on - strand, within EX31_RS09240 at 46.749 kb on - strand, within EX31_RS09240 at 46.846 kb on + strand, within EX31_RS09240 at 46.846 kb on + strand, within EX31_RS09240
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 44,480 - EX31_RS09230 0.18 +1.7 44,551 + EX31_RS09230 0.26 +2.0 44,723 + EX31_RS09230 0.45 +0.1 44,962 - EX31_RS09230 0.72 +0.5 44,962 - EX31_RS09230 0.72 +0.4 44,962 - EX31_RS09230 0.72 +0.1 44,994 - EX31_RS09230 0.75 -1.1 44,994 - EX31_RS09230 0.75 +0.5 45,066 - EX31_RS09230 0.83 +0.7 45,177 + +0.6 45,207 + +0.3 45,225 - -1.6 45,271 - +0.4 45,274 - +1.3 45,274 - +0.3 45,530 + EX31_RS09235 0.37 -0.5 45,530 + EX31_RS09235 0.37 +0.4 45,530 + EX31_RS09235 0.37 +0.9 45,531 - EX31_RS09235 0.37 +1.3 45,546 - EX31_RS09235 0.39 -0.2 45,631 + EX31_RS09235 0.50 +2.5 45,631 + EX31_RS09235 0.50 +0.3 45,631 + EX31_RS09235 0.50 +0.7 45,631 + EX31_RS09235 0.50 +1.7 45,632 - EX31_RS09235 0.50 +0.8 45,632 - EX31_RS09235 0.50 -0.1 45,759 - EX31_RS09235 0.65 +0.0 46,084 - +1.0 46,137 - +0.9 46,150 - +1.0 46,153 + +0.5 46,154 - +0.4 46,154 - +1.0 46,227 + -0.2 46,228 - -0.4 46,443 + EX31_RS09240 0.29 -0.6 46,565 - EX31_RS09240 0.40 +1.4 46,571 - EX31_RS09240 0.41 +1.7 46,624 + EX31_RS09240 0.46 +0.8 46,625 - EX31_RS09240 0.46 +0.4 46,744 - EX31_RS09240 0.57 +0.9 46,744 - EX31_RS09240 0.57 +0.7 46,748 + EX31_RS09240 0.58 -0.2 46,748 + EX31_RS09240 0.58 -0.8 46,749 - EX31_RS09240 0.58 +1.7 46,749 - EX31_RS09240 0.58 +0.3 46,846 + EX31_RS09240 0.67 -2.6 46,846 + EX31_RS09240 0.67 -1.0
Or see this region's nucleotide sequence