Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07750 and EX31_RS07755 are separated by 85 nucleotides EX31_RS07755 and EX31_RS07760 overlap by 4 nucleotides EX31_RS07760 and EX31_RS07765 are separated by 355 nucleotides EX31_RS07765 and EX31_RS07770 are separated by 42 nucleotides EX31_RS07770 and EX31_RS07775 are separated by 110 nucleotides EX31_RS07775 and EX31_RS07780 are separated by 8 nucleotides
EX31_RS07750: EX31_RS07750 - glutamate--cysteine ligase, at 31,890 to 33,461
_RS07750
EX31_RS07755: EX31_RS07755 - DedA family protein, at 33,547 to 33,975
_RS07755
EX31_RS07760: EX31_RS07760 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 33,972 to 34,538
_RS07760
EX31_RS07765: EX31_RS07765 - tRNA-Arg, at 34,894 to 34,970
_RS07765
EX31_RS07770: EX31_RS07770 - tRNA-Arg, at 35,013 to 35,089
_RS07770
EX31_RS07775: EX31_RS07775 - tRNA-Arg, at 35,200 to 35,276
_RS07775
EX31_RS07780: EX31_RS07780 - tRNA-Ser, at 35,285 to 35,377
_RS07780
Position (kb) 33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 33.010 kb on + strand, within EX31_RS07750 at 33.028 kb on - strand, within EX31_RS07750 at 33.106 kb on + strand, within EX31_RS07750 at 33.107 kb on - strand, within EX31_RS07750 at 33.167 kb on + strand, within EX31_RS07750 at 33.167 kb on + strand, within EX31_RS07750 at 33.167 kb on + strand, within EX31_RS07750 at 33.167 kb on + strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.168 kb on - strand, within EX31_RS07750 at 33.236 kb on + strand, within EX31_RS07750 at 33.236 kb on + strand, within EX31_RS07750 at 33.237 kb on - strand, within EX31_RS07750 at 33.257 kb on + strand, within EX31_RS07750 at 33.258 kb on - strand, within EX31_RS07750 at 33.258 kb on - strand, within EX31_RS07750 at 33.264 kb on + strand, within EX31_RS07750 at 33.267 kb on + strand, within EX31_RS07750 at 33.319 kb on - strand at 33.398 kb on + strand at 33.405 kb on + strand at 33.406 kb on - strand at 33.447 kb on + strand at 33.447 kb on + strand at 33.447 kb on + strand at 33.448 kb on - strand at 33.448 kb on - strand at 33.448 kb on - strand at 33.448 kb on - strand at 33.489 kb on - strand at 33.501 kb on + strand at 33.501 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.514 kb on + strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.515 kb on - strand at 33.526 kb on + strand at 33.527 kb on - strand at 33.527 kb on - strand at 33.527 kb on - strand at 33.533 kb on + strand at 33.533 kb on + strand at 33.548 kb on + strand at 33.578 kb on - strand at 33.596 kb on + strand, within EX31_RS07755 at 33.596 kb on + strand, within EX31_RS07755 at 33.596 kb on + strand, within EX31_RS07755 at 33.596 kb on + strand, within EX31_RS07755 at 33.596 kb on + strand, within EX31_RS07755 at 33.597 kb on - strand, within EX31_RS07755 at 33.597 kb on - strand, within EX31_RS07755 at 33.665 kb on - strand, within EX31_RS07755 at 33.667 kb on + strand, within EX31_RS07755 at 33.667 kb on + strand, within EX31_RS07755 at 33.667 kb on + strand, within EX31_RS07755 at 33.667 kb on + strand, within EX31_RS07755 at 33.668 kb on - strand, within EX31_RS07755 at 33.668 kb on - strand, within EX31_RS07755 at 33.673 kb on + strand, within EX31_RS07755 at 33.674 kb on - strand, within EX31_RS07755 at 33.710 kb on - strand, within EX31_RS07755 at 33.771 kb on + strand, within EX31_RS07755 at 33.772 kb on - strand, within EX31_RS07755 at 33.772 kb on - strand, within EX31_RS07755 at 33.772 kb on - strand, within EX31_RS07755 at 33.809 kb on - strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.817 kb on + strand, within EX31_RS07755 at 33.818 kb on - strand, within EX31_RS07755 at 33.818 kb on - strand, within EX31_RS07755 at 33.818 kb on - strand, within EX31_RS07755 at 33.818 kb on - strand, within EX31_RS07755 at 33.818 kb on - strand, within EX31_RS07755 at 33.822 kb on + strand, within EX31_RS07755 at 33.823 kb on - strand, within EX31_RS07755 at 33.890 kb on - strand, within EX31_RS07755 at 33.890 kb on - strand, within EX31_RS07755 at 33.892 kb on + strand, within EX31_RS07755 at 33.893 kb on - strand, within EX31_RS07755 at 33.917 kb on - strand, within EX31_RS07755 at 33.928 kb on + strand, within EX31_RS07755 at 33.929 kb on - strand, within EX31_RS07755 at 33.980 kb on + strand at 34.001 kb on + strand at 34.001 kb on + strand at 34.001 kb on + strand at 34.171 kb on - strand, within EX31_RS07760 at 34.171 kb on - strand, within EX31_RS07760 at 34.171 kb on - strand, within EX31_RS07760 at 34.243 kb on + strand, within EX31_RS07760 at 34.243 kb on + strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.244 kb on - strand, within EX31_RS07760 at 34.278 kb on + strand, within EX31_RS07760 at 34.279 kb on - strand, within EX31_RS07760 at 34.279 kb on - strand, within EX31_RS07760 at 34.279 kb on - strand, within EX31_RS07760 at 34.352 kb on + strand, within EX31_RS07760 at 34.352 kb on + strand, within EX31_RS07760 at 34.352 kb on + strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.353 kb on - strand, within EX31_RS07760 at 34.355 kb on + strand, within EX31_RS07760 at 34.355 kb on + strand, within EX31_RS07760 at 34.356 kb on - strand, within EX31_RS07760 at 34.356 kb on - strand, within EX31_RS07760 at 34.356 kb on - strand, within EX31_RS07760 at 34.356 kb on - strand, within EX31_RS07760 at 34.362 kb on + strand, within EX31_RS07760 at 34.362 kb on + strand, within EX31_RS07760 at 34.363 kb on - strand, within EX31_RS07760 at 34.364 kb on + strand, within EX31_RS07760 at 34.365 kb on - strand, within EX31_RS07760 at 34.426 kb on + strand, within EX31_RS07760 at 34.427 kb on - strand, within EX31_RS07760 at 34.438 kb on + strand, within EX31_RS07760 at 34.438 kb on + strand, within EX31_RS07760 at 34.438 kb on + strand, within EX31_RS07760 at 34.439 kb on - strand, within EX31_RS07760 at 34.439 kb on - strand, within EX31_RS07760 at 34.439 kb on - strand, within EX31_RS07760 at 34.439 kb on - strand, within EX31_RS07760 at 34.476 kb on - strand, within EX31_RS07760 at 34.476 kb on - strand, within EX31_RS07760 at 34.476 kb on - strand, within EX31_RS07760 at 34.476 kb on - strand, within EX31_RS07760 at 34.476 kb on - strand, within EX31_RS07760 at 34.492 kb on - strand at 34.492 kb on - strand at 34.492 kb on - strand at 34.505 kb on + strand at 34.512 kb on + strand at 34.512 kb on + strand at 34.513 kb on - strand at 34.513 kb on - strand at 34.516 kb on - strand at 34.526 kb on - strand at 34.534 kb on + strand at 34.534 kb on + strand at 34.535 kb on - strand at 34.535 kb on - strand at 34.574 kb on - strand at 34.574 kb on - strand at 34.574 kb on - strand at 34.574 kb on - strand at 34.592 kb on + strand at 34.593 kb on - strand at 34.672 kb on + strand at 34.673 kb on - strand at 34.673 kb on - strand at 34.721 kb on - strand at 34.750 kb on + strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.751 kb on - strand at 34.760 kb on - strand at 34.760 kb on - strand at 34.760 kb on - strand at 34.760 kb on - strand at 34.813 kb on - strand at 34.824 kb on - strand at 34.824 kb on - strand at 34.824 kb on - strand at 34.824 kb on - strand at 34.824 kb on - strand at 34.824 kb on - strand at 34.860 kb on - strand at 34.860 kb on - strand at 34.860 kb on - strand at 34.860 kb on - strand at 34.860 kb on - strand at 34.862 kb on - strand at 34.862 kb on - strand at 34.862 kb on - strand at 34.888 kb on - strand at 34.888 kb on - strand at 34.888 kb on - strand at 34.888 kb on - strand at 34.888 kb on - strand at 34.893 kb on - strand at 34.893 kb on - strand at 34.893 kb on - strand at 34.944 kb on + strand, within EX31_RS07765 at 34.979 kb on - strand at 34.991 kb on - strand at 34.991 kb on - strand at 35.102 kb on - strand at 35.113 kb on - strand at 35.126 kb on - strand at 35.126 kb on - strand at 35.131 kb on - strand at 35.131 kb on - strand at 35.131 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.185 kb on - strand at 35.199 kb on - strand at 35.199 kb on - strand at 35.199 kb on - strand at 35.380 kb on - strand at 35.533 kb on + strand at 35.534 kb on - strand at 35.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 33,010 + EX31_RS07750 0.71 +0.5 33,028 - EX31_RS07750 0.72 -0.1 33,106 + EX31_RS07750 0.77 -1.1 33,107 - EX31_RS07750 0.77 -0.9 33,167 + EX31_RS07750 0.81 +0.6 33,167 + EX31_RS07750 0.81 -1.5 33,167 + EX31_RS07750 0.81 -1.0 33,167 + EX31_RS07750 0.81 -1.4 33,168 - EX31_RS07750 0.81 -0.9 33,168 - EX31_RS07750 0.81 +0.5 33,168 - EX31_RS07750 0.81 +1.2 33,168 - EX31_RS07750 0.81 -0.4 33,168 - EX31_RS07750 0.81 -0.2 33,168 - EX31_RS07750 0.81 -0.9 33,168 - EX31_RS07750 0.81 +1.1 33,168 - EX31_RS07750 0.81 +0.5 33,168 - EX31_RS07750 0.81 +0.3 33,168 - EX31_RS07750 0.81 +0.9 33,168 - EX31_RS07750 0.81 +1.2 33,236 + EX31_RS07750 0.86 +0.2 33,236 + EX31_RS07750 0.86 +0.6 33,237 - EX31_RS07750 0.86 -1.0 33,257 + EX31_RS07750 0.87 +0.3 33,258 - EX31_RS07750 0.87 -0.5 33,258 - EX31_RS07750 0.87 -0.9 33,264 + EX31_RS07750 0.87 -0.6 33,267 + EX31_RS07750 0.88 -0.6 33,319 - -1.0 33,398 + +1.5 33,405 + -0.5 33,406 - -0.5 33,447 + -0.3 33,447 + -1.1 33,447 + -0.2 33,448 - +0.6 33,448 - +0.5 33,448 - -0.1 33,448 - -0.5 33,489 - +0.5 33,501 + -0.1 33,501 + -0.6 33,514 + -1.2 33,514 + -3.2 33,514 + +0.3 33,514 + +0.4 33,514 + +0.2 33,514 + +1.4 33,514 + -0.3 33,514 + -0.1 33,514 + -2.1 33,514 + +0.4 33,515 - +0.1 33,515 - -0.4 33,515 - +0.4 33,515 - -0.4 33,515 - -1.4 33,515 - -0.0 33,515 - -0.2 33,515 - +1.3 33,515 - +0.5 33,515 - +0.5 33,515 - +0.9 33,526 + -0.0 33,527 - -1.0 33,527 - +1.2 33,527 - -0.6 33,533 + +0.9 33,533 + -1.1 33,548 + +3.0 33,578 - +0.1 33,596 + EX31_RS07755 0.11 +0.6 33,596 + EX31_RS07755 0.11 +0.2 33,596 + EX31_RS07755 0.11 +0.9 33,596 + EX31_RS07755 0.11 -0.5 33,596 + EX31_RS07755 0.11 -0.4 33,597 - EX31_RS07755 0.12 -1.1 33,597 - EX31_RS07755 0.12 +0.1 33,665 - EX31_RS07755 0.28 +0.7 33,667 + EX31_RS07755 0.28 +0.6 33,667 + EX31_RS07755 0.28 -0.9 33,667 + EX31_RS07755 0.28 -0.9 33,667 + EX31_RS07755 0.28 +0.1 33,668 - EX31_RS07755 0.28 -0.2 33,668 - EX31_RS07755 0.28 +1.5 33,673 + EX31_RS07755 0.29 +0.9 33,674 - EX31_RS07755 0.30 +0.5 33,710 - EX31_RS07755 0.38 +0.3 33,771 + EX31_RS07755 0.52 -0.2 33,772 - EX31_RS07755 0.52 -0.3 33,772 - EX31_RS07755 0.52 -0.2 33,772 - EX31_RS07755 0.52 +0.0 33,809 - EX31_RS07755 0.61 -0.0 33,817 + EX31_RS07755 0.63 +0.5 33,817 + EX31_RS07755 0.63 +0.3 33,817 + EX31_RS07755 0.63 -0.2 33,817 + EX31_RS07755 0.63 +1.0 33,817 + EX31_RS07755 0.63 -0.1 33,817 + EX31_RS07755 0.63 +1.2 33,818 - EX31_RS07755 0.63 -0.0 33,818 - EX31_RS07755 0.63 -0.9 33,818 - EX31_RS07755 0.63 +0.3 33,818 - EX31_RS07755 0.63 +1.4 33,818 - EX31_RS07755 0.63 -1.3 33,822 + EX31_RS07755 0.64 +2.5 33,823 - EX31_RS07755 0.64 -0.2 33,890 - EX31_RS07755 0.80 -0.2 33,890 - EX31_RS07755 0.80 -0.4 33,892 + EX31_RS07755 0.80 +0.5 33,893 - EX31_RS07755 0.81 +0.4 33,917 - EX31_RS07755 0.86 -1.0 33,928 + EX31_RS07755 0.89 -0.4 33,929 - EX31_RS07755 0.89 +0.3 33,980 + -0.3 34,001 + +2.5 34,001 + +1.5 34,001 + -0.1 34,171 - EX31_RS07760 0.35 +0.4 34,171 - EX31_RS07760 0.35 +1.1 34,171 - EX31_RS07760 0.35 -1.0 34,243 + EX31_RS07760 0.48 +0.4 34,243 + EX31_RS07760 0.48 +0.3 34,244 - EX31_RS07760 0.48 -0.6 34,244 - EX31_RS07760 0.48 -1.0 34,244 - EX31_RS07760 0.48 -0.9 34,244 - EX31_RS07760 0.48 +0.2 34,244 - EX31_RS07760 0.48 -0.7 34,244 - EX31_RS07760 0.48 +0.7 34,278 + EX31_RS07760 0.54 -0.0 34,279 - EX31_RS07760 0.54 +0.5 34,279 - EX31_RS07760 0.54 +1.4 34,279 - EX31_RS07760 0.54 -1.9 34,352 + EX31_RS07760 0.67 +0.4 34,352 + EX31_RS07760 0.67 +1.5 34,352 + EX31_RS07760 0.67 -0.9 34,353 - EX31_RS07760 0.67 +0.8 34,353 - EX31_RS07760 0.67 +0.3 34,353 - EX31_RS07760 0.67 -0.2 34,353 - EX31_RS07760 0.67 +0.6 34,353 - EX31_RS07760 0.67 +1.1 34,353 - EX31_RS07760 0.67 +2.5 34,353 - EX31_RS07760 0.67 +1.0 34,353 - EX31_RS07760 0.67 -0.3 34,355 + EX31_RS07760 0.68 +0.7 34,355 + EX31_RS07760 0.68 +1.1 34,356 - EX31_RS07760 0.68 -0.3 34,356 - EX31_RS07760 0.68 +0.7 34,356 - EX31_RS07760 0.68 +0.3 34,356 - EX31_RS07760 0.68 -0.1 34,362 + EX31_RS07760 0.69 +0.4 34,362 + EX31_RS07760 0.69 +0.8 34,363 - EX31_RS07760 0.69 -0.1 34,364 + EX31_RS07760 0.69 +0.7 34,365 - EX31_RS07760 0.69 -0.1 34,426 + EX31_RS07760 0.80 +0.5 34,427 - EX31_RS07760 0.80 +0.7 34,438 + EX31_RS07760 0.82 +1.0 34,438 + EX31_RS07760 0.82 +0.7 34,438 + EX31_RS07760 0.82 -1.5 34,439 - EX31_RS07760 0.82 +0.3 34,439 - EX31_RS07760 0.82 -0.4 34,439 - EX31_RS07760 0.82 -1.6 34,439 - EX31_RS07760 0.82 +0.6 34,476 - EX31_RS07760 0.89 -0.4 34,476 - EX31_RS07760 0.89 +0.2 34,476 - EX31_RS07760 0.89 +0.6 34,476 - EX31_RS07760 0.89 +0.8 34,476 - EX31_RS07760 0.89 +0.6 34,492 - -0.4 34,492 - -0.0 34,492 - +1.7 34,505 + +1.7 34,512 + -0.2 34,512 + -0.6 34,513 - -0.4 34,513 - +0.4 34,516 - -0.2 34,526 - +0.7 34,534 + +0.6 34,534 + +0.3 34,535 - +0.7 34,535 - -1.4 34,574 - +0.4 34,574 - -0.5 34,574 - -0.8 34,574 - +2.3 34,592 + +0.9 34,593 - +0.6 34,672 + +0.4 34,673 - -0.6 34,673 - +0.5 34,721 - +0.6 34,750 + +1.5 34,751 - +0.7 34,751 - +0.5 34,751 - -0.2 34,751 - -0.6 34,751 - +0.9 34,751 - +0.1 34,751 - +0.4 34,751 - +0.5 34,760 - +0.5 34,760 - +1.1 34,760 - -0.3 34,760 - -1.8 34,813 - +1.1 34,824 - -0.6 34,824 - +0.9 34,824 - -0.5 34,824 - +0.2 34,824 - +0.5 34,824 - -0.2 34,860 - -0.3 34,860 - -0.5 34,860 - +1.1 34,860 - -0.8 34,860 - +1.5 34,862 - +0.0 34,862 - +0.8 34,862 - -0.3 34,888 - +0.1 34,888 - +0.4 34,888 - +1.5 34,888 - -0.0 34,888 - +0.4 34,893 - -0.4 34,893 - +0.3 34,893 - +0.1 34,944 + EX31_RS07765 0.65 -0.3 34,979 - -1.6 34,991 - -0.4 34,991 - +1.2 35,102 - -0.5 35,113 - -0.1 35,126 - -0.9 35,126 - +0.6 35,131 - +0.7 35,131 - +1.0 35,131 - -0.3 35,185 - +0.8 35,185 - -0.1 35,185 - -0.0 35,185 - -0.6 35,185 - +1.0 35,185 - +0.4 35,185 - -1.3 35,185 - -1.1 35,185 - +0.9 35,199 - +0.8 35,199 - +0.5 35,199 - -0.1 35,380 - -0.8 35,533 + +0.6 35,534 - +0.0 35,534 - -0.2
Or see this region's nucleotide sequence