Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06315 and EX31_RS06320 overlap by 8 nucleotides EX31_RS06320 and EX31_RS06325 are separated by 74 nucleotides EX31_RS06325 and EX31_RS06330 overlap by 1 nucleotides
EX31_RS06315: EX31_RS06315 - DNA topoisomerase III, at 25,575 to 27,527
_RS06315
EX31_RS06320: EX31_RS06320 - YnjH family protein, at 27,520 to 27,834
_RS06320
EX31_RS06325: EX31_RS06325 - pyrimidine (deoxy)nucleoside triphosphate diphosphatase, at 27,909 to 28,310
_RS06325
EX31_RS06330: EX31_RS06330 - exodeoxyribonuclease III, at 28,310 to 29,119
_RS06330
Position (kb)
27
28 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 26.555 kb on + strand, within EX31_RS06315 at 26.555 kb on + strand, within EX31_RS06315 at 26.555 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.680 kb on + strand, within EX31_RS06315 at 26.750 kb on + strand, within EX31_RS06315 at 26.751 kb on - strand, within EX31_RS06315 at 26.805 kb on + strand, within EX31_RS06315 at 26.805 kb on + strand, within EX31_RS06315 at 26.806 kb on - strand, within EX31_RS06315 at 27.050 kb on + strand, within EX31_RS06315 at 27.051 kb on - strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.059 kb on + strand, within EX31_RS06315 at 27.060 kb on - strand, within EX31_RS06315 at 27.104 kb on + strand, within EX31_RS06315 at 27.142 kb on + strand, within EX31_RS06315 at 27.216 kb on + strand, within EX31_RS06315 at 27.217 kb on - strand, within EX31_RS06315 at 27.217 kb on - strand, within EX31_RS06315 at 27.218 kb on + strand, within EX31_RS06315 at 27.218 kb on + strand, within EX31_RS06315 at 27.219 kb on - strand, within EX31_RS06315 at 27.219 kb on - strand, within EX31_RS06315 at 27.477 kb on - strand at 27.495 kb on - strand at 27.621 kb on + strand, within EX31_RS06320 at 27.678 kb on + strand, within EX31_RS06320 at 27.678 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.708 kb on + strand, within EX31_RS06320 at 27.709 kb on - strand, within EX31_RS06320 at 27.709 kb on - strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.712 kb on + strand, within EX31_RS06320 at 27.727 kb on + strand, within EX31_RS06320 at 27.843 kb on + strand at 27.895 kb on - strand at 27.895 kb on - strand at 27.899 kb on - strand at 28.026 kb on - strand, within EX31_RS06325 at 28.072 kb on - strand, within EX31_RS06325 at 28.091 kb on + strand, within EX31_RS06325 at 28.092 kb on - strand, within EX31_RS06325 at 28.158 kb on + strand, within EX31_RS06325 at 28.158 kb on + strand, within EX31_RS06325 at 28.159 kb on - strand, within EX31_RS06325 at 28.159 kb on - strand, within EX31_RS06325 at 28.254 kb on + strand, within EX31_RS06325 at 28.255 kb on - strand, within EX31_RS06325 at 28.311 kb on + strand at 28.311 kb on + strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.312 kb on - strand at 28.378 kb on - strand at 28.378 kb on - strand at 28.378 kb on - strand at 28.439 kb on - strand, within EX31_RS06330 at 28.496 kb on - strand, within EX31_RS06330 at 28.661 kb on - strand, within EX31_RS06330 at 28.661 kb on - strand, within EX31_RS06330 at 28.795 kb on - strand, within EX31_RS06330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 26,555 + EX31_RS06315 0.50 +0.5 26,555 + EX31_RS06315 0.50 -0.5 26,555 + EX31_RS06315 0.50 -0.6 26,680 + EX31_RS06315 0.57 +1.1 26,680 + EX31_RS06315 0.57 -0.2 26,680 + EX31_RS06315 0.57 -0.7 26,680 + EX31_RS06315 0.57 +1.6 26,750 + EX31_RS06315 0.60 +2.2 26,751 - EX31_RS06315 0.60 +0.3 26,805 + EX31_RS06315 0.63 -0.2 26,805 + EX31_RS06315 0.63 +0.4 26,806 - EX31_RS06315 0.63 -1.2 27,050 + EX31_RS06315 0.76 -0.4 27,051 - EX31_RS06315 0.76 -0.4 27,059 + EX31_RS06315 0.76 -0.8 27,059 + EX31_RS06315 0.76 +0.6 27,059 + EX31_RS06315 0.76 -0.0 27,059 + EX31_RS06315 0.76 -0.8 27,059 + EX31_RS06315 0.76 -0.8 27,059 + EX31_RS06315 0.76 +0.1 27,060 - EX31_RS06315 0.76 +2.0 27,104 + EX31_RS06315 0.78 +2.6 27,142 + EX31_RS06315 0.80 +0.6 27,216 + EX31_RS06315 0.84 +0.1 27,217 - EX31_RS06315 0.84 -0.4 27,217 - EX31_RS06315 0.84 +0.2 27,218 + EX31_RS06315 0.84 -0.4 27,218 + EX31_RS06315 0.84 +0.7 27,219 - EX31_RS06315 0.84 +0.3 27,219 - EX31_RS06315 0.84 -1.4 27,477 - -1.0 27,495 - -0.0 27,621 + EX31_RS06320 0.32 -1.2 27,678 + EX31_RS06320 0.50 -0.4 27,678 + EX31_RS06320 0.50 -0.6 27,708 + EX31_RS06320 0.60 +2.4 27,708 + EX31_RS06320 0.60 -1.3 27,708 + EX31_RS06320 0.60 +0.1 27,709 - EX31_RS06320 0.60 -0.9 27,709 - EX31_RS06320 0.60 +0.1 27,712 + EX31_RS06320 0.61 -0.2 27,712 + EX31_RS06320 0.61 -0.4 27,712 + EX31_RS06320 0.61 +0.7 27,712 + EX31_RS06320 0.61 -0.0 27,712 + EX31_RS06320 0.61 -0.3 27,727 + EX31_RS06320 0.66 -0.4 27,843 + -0.2 27,895 - -0.3 27,895 - +1.4 27,899 - -0.1 28,026 - EX31_RS06325 0.29 -1.4 28,072 - EX31_RS06325 0.41 -0.6 28,091 + EX31_RS06325 0.45 +0.2 28,092 - EX31_RS06325 0.46 -0.2 28,158 + EX31_RS06325 0.62 +3.6 28,158 + EX31_RS06325 0.62 -1.4 28,159 - EX31_RS06325 0.62 -0.7 28,159 - EX31_RS06325 0.62 +0.6 28,254 + EX31_RS06325 0.86 -2.0 28,255 - EX31_RS06325 0.86 -0.4 28,311 + +0.3 28,311 + +0.6 28,312 - +0.6 28,312 - +1.6 28,312 - +0.1 28,312 - -0.4 28,378 - +0.6 28,378 - +1.6 28,378 - -1.8 28,439 - EX31_RS06330 0.16 -1.4 28,496 - EX31_RS06330 0.23 -2.1 28,661 - EX31_RS06330 0.43 +0.3 28,661 - EX31_RS06330 0.43 +4.4 28,795 - EX31_RS06330 0.60 -0.0
Or see this region's nucleotide sequence