Experiment: R2A with nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09720 and CA265_RS09725 are separated by 174 nucleotides CA265_RS09725 and CA265_RS09730 are separated by 31 nucleotides CA265_RS09730 and CA265_RS09735 are separated by 91 nucleotides
CA265_RS09720: CA265_RS09720 - transglycosylase, at 2,395,305 to 2,397,755
_RS09720
CA265_RS09725: CA265_RS09725 - tolB protein precursor, at 2,397,930 to 2,401,118
_RS09725
CA265_RS09730: CA265_RS09730 - subclass B3 metallo-beta-lactamase, at 2,401,150 to 2,402,007
_RS09730
CA265_RS09735: CA265_RS09735 - nuclease, at 2,402,099 to 2,402,449
_RS09735
Position (kb)
2397
2398
2399
2400
2401
2402 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2396.938 kb on + strand, within CA265_RS09720 at 2397.146 kb on + strand, within CA265_RS09720 at 2397.265 kb on + strand, within CA265_RS09720 at 2397.277 kb on - strand, within CA265_RS09720 at 2397.286 kb on + strand, within CA265_RS09720 at 2397.413 kb on - strand, within CA265_RS09720 at 2397.437 kb on + strand, within CA265_RS09720 at 2397.441 kb on + strand, within CA265_RS09720 at 2397.470 kb on + strand, within CA265_RS09720 at 2397.515 kb on + strand at 2397.516 kb on - strand at 2397.535 kb on + strand at 2397.660 kb on - strand at 2397.768 kb on + strand at 2397.781 kb on - strand at 2397.789 kb on + strand at 2397.790 kb on - strand at 2397.811 kb on + strand at 2397.839 kb on + strand at 2397.865 kb on - strand at 2397.929 kb on + strand at 2397.930 kb on - strand at 2397.930 kb on - strand at 2397.944 kb on + strand at 2397.949 kb on + strand at 2397.950 kb on - strand at 2397.970 kb on + strand at 2397.971 kb on - strand at 2397.984 kb on - strand at 2398.012 kb on - strand at 2398.030 kb on + strand at 2398.031 kb on - strand at 2398.087 kb on - strand at 2398.087 kb on - strand at 2398.089 kb on + strand at 2398.089 kb on + strand at 2398.172 kb on + strand at 2398.204 kb on - strand at 2398.208 kb on + strand at 2398.209 kb on - strand at 2398.301 kb on - strand, within CA265_RS09725 at 2398.400 kb on + strand, within CA265_RS09725 at 2398.401 kb on - strand, within CA265_RS09725 at 2398.535 kb on - strand at 2398.596 kb on + strand, within CA265_RS09725 at 2398.596 kb on + strand, within CA265_RS09725 at 2398.597 kb on - strand, within CA265_RS09725 at 2398.600 kb on + strand, within CA265_RS09725 at 2398.601 kb on - strand, within CA265_RS09725 at 2398.626 kb on - strand, within CA265_RS09725 at 2398.656 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.756 kb on + strand, within CA265_RS09725 at 2398.757 kb on - strand, within CA265_RS09725 at 2398.757 kb on - strand, within CA265_RS09725 at 2398.823 kb on + strand, within CA265_RS09725 at 2398.823 kb on + strand, within CA265_RS09725 at 2398.861 kb on - strand, within CA265_RS09725 at 2398.871 kb on + strand, within CA265_RS09725 at 2398.904 kb on - strand, within CA265_RS09725 at 2398.946 kb on - strand, within CA265_RS09725 at 2398.989 kb on - strand, within CA265_RS09725 at 2398.991 kb on - strand, within CA265_RS09725 at 2399.019 kb on - strand, within CA265_RS09725 at 2399.037 kb on - strand, within CA265_RS09725 at 2399.072 kb on + strand, within CA265_RS09725 at 2399.093 kb on + strand, within CA265_RS09725 at 2399.093 kb on + strand, within CA265_RS09725 at 2399.093 kb on + strand, within CA265_RS09725 at 2399.218 kb on + strand, within CA265_RS09725 at 2399.218 kb on + strand, within CA265_RS09725 at 2399.219 kb on - strand, within CA265_RS09725 at 2399.219 kb on - strand, within CA265_RS09725 at 2399.219 kb on - strand, within CA265_RS09725 at 2399.219 kb on - strand, within CA265_RS09725 at 2399.255 kb on - strand, within CA265_RS09725 at 2399.256 kb on + strand, within CA265_RS09725 at 2399.256 kb on + strand, within CA265_RS09725 at 2399.315 kb on + strand, within CA265_RS09725 at 2399.316 kb on - strand, within CA265_RS09725 at 2399.338 kb on + strand, within CA265_RS09725 at 2399.343 kb on - strand, within CA265_RS09725 at 2399.351 kb on - strand, within CA265_RS09725 at 2399.353 kb on + strand, within CA265_RS09725 at 2399.354 kb on - strand, within CA265_RS09725 at 2399.405 kb on - strand, within CA265_RS09725 at 2399.432 kb on - strand, within CA265_RS09725 at 2399.462 kb on + strand, within CA265_RS09725 at 2399.500 kb on + strand, within CA265_RS09725 at 2399.503 kb on - strand, within CA265_RS09725 at 2399.525 kb on + strand, within CA265_RS09725 at 2399.603 kb on + strand, within CA265_RS09725 at 2399.613 kb on - strand, within CA265_RS09725 at 2399.617 kb on + strand, within CA265_RS09725 at 2399.618 kb on - strand, within CA265_RS09725 at 2399.618 kb on - strand, within CA265_RS09725 at 2399.619 kb on + strand, within CA265_RS09725 at 2399.653 kb on - strand, within CA265_RS09725 at 2399.653 kb on - strand, within CA265_RS09725 at 2399.749 kb on + strand, within CA265_RS09725 at 2399.750 kb on - strand, within CA265_RS09725 at 2399.822 kb on - strand, within CA265_RS09725 at 2399.826 kb on + strand, within CA265_RS09725 at 2399.826 kb on + strand, within CA265_RS09725 at 2399.963 kb on + strand, within CA265_RS09725 at 2400.056 kb on + strand, within CA265_RS09725 at 2400.063 kb on + strand, within CA265_RS09725 at 2400.068 kb on - strand, within CA265_RS09725 at 2400.078 kb on - strand, within CA265_RS09725 at 2400.106 kb on + strand, within CA265_RS09725 at 2400.107 kb on - strand, within CA265_RS09725 at 2400.107 kb on - strand, within CA265_RS09725 at 2400.107 kb on - strand, within CA265_RS09725 at 2400.165 kb on - strand, within CA265_RS09725 at 2400.168 kb on - strand, within CA265_RS09725 at 2400.168 kb on - strand, within CA265_RS09725 at 2400.260 kb on + strand, within CA265_RS09725 at 2400.260 kb on + strand, within CA265_RS09725 at 2400.261 kb on - strand, within CA265_RS09725 at 2400.288 kb on + strand, within CA265_RS09725 at 2400.309 kb on + strand, within CA265_RS09725 at 2400.309 kb on + strand, within CA265_RS09725 at 2400.339 kb on - strand, within CA265_RS09725 at 2400.419 kb on + strand, within CA265_RS09725 at 2400.420 kb on - strand, within CA265_RS09725 at 2400.458 kb on + strand, within CA265_RS09725 at 2400.458 kb on + strand, within CA265_RS09725 at 2400.459 kb on - strand, within CA265_RS09725 at 2400.469 kb on + strand, within CA265_RS09725 at 2400.495 kb on + strand, within CA265_RS09725 at 2400.570 kb on + strand, within CA265_RS09725 at 2400.571 kb on - strand, within CA265_RS09725 at 2400.602 kb on + strand, within CA265_RS09725 at 2400.618 kb on + strand, within CA265_RS09725 at 2400.619 kb on - strand, within CA265_RS09725 at 2400.634 kb on - strand, within CA265_RS09725 at 2400.675 kb on - strand, within CA265_RS09725 at 2400.675 kb on - strand, within CA265_RS09725 at 2400.708 kb on - strand, within CA265_RS09725 at 2400.715 kb on + strand, within CA265_RS09725 at 2400.773 kb on + strand, within CA265_RS09725 at 2400.791 kb on - strand, within CA265_RS09725 at 2400.818 kb on - strand at 2400.890 kb on + strand at 2400.890 kb on + strand at 2400.999 kb on - strand at 2400.999 kb on - strand at 2401.000 kb on + strand at 2401.001 kb on - strand at 2401.003 kb on - strand at 2401.045 kb on - strand at 2401.045 kb on - strand at 2401.047 kb on + strand at 2401.212 kb on - strand at 2401.227 kb on + strand at 2401.264 kb on - strand, within CA265_RS09730 at 2401.312 kb on + strand, within CA265_RS09730 at 2401.317 kb on - strand, within CA265_RS09730 at 2401.317 kb on - strand, within CA265_RS09730 at 2401.339 kb on - strand, within CA265_RS09730 at 2401.339 kb on - strand, within CA265_RS09730 at 2401.413 kb on + strand, within CA265_RS09730 at 2401.436 kb on + strand, within CA265_RS09730 at 2401.436 kb on + strand, within CA265_RS09730 at 2401.502 kb on + strand, within CA265_RS09730 at 2401.504 kb on + strand, within CA265_RS09730 at 2401.504 kb on + strand, within CA265_RS09730 at 2401.505 kb on - strand, within CA265_RS09730 at 2401.555 kb on + strand, within CA265_RS09730 at 2401.577 kb on + strand, within CA265_RS09730 at 2401.635 kb on - strand, within CA265_RS09730 at 2401.645 kb on - strand, within CA265_RS09730 at 2401.645 kb on - strand, within CA265_RS09730 at 2401.650 kb on - strand, within CA265_RS09730 at 2401.674 kb on + strand, within CA265_RS09730 at 2401.696 kb on - strand, within CA265_RS09730 at 2401.698 kb on - strand, within CA265_RS09730 at 2401.698 kb on - strand, within CA265_RS09730 at 2401.702 kb on - strand, within CA265_RS09730 at 2401.747 kb on - strand, within CA265_RS09730 at 2401.758 kb on - strand, within CA265_RS09730 at 2401.785 kb on - strand, within CA265_RS09730 at 2401.792 kb on + strand, within CA265_RS09730 at 2401.813 kb on + strand, within CA265_RS09730 at 2401.816 kb on - strand, within CA265_RS09730 at 2401.896 kb on + strand, within CA265_RS09730 at 2401.942 kb on - strand at 2402.014 kb on - strand at 2402.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 100 mM remove 2,396,938 + CA265_RS09720 0.67 +0.0 2,397,146 + CA265_RS09720 0.75 -0.9 2,397,265 + CA265_RS09720 0.80 +0.8 2,397,277 - CA265_RS09720 0.80 -0.5 2,397,286 + CA265_RS09720 0.81 +1.1 2,397,413 - CA265_RS09720 0.86 +1.3 2,397,437 + CA265_RS09720 0.87 +0.7 2,397,441 + CA265_RS09720 0.87 -0.5 2,397,470 + CA265_RS09720 0.88 -0.3 2,397,515 + -0.1 2,397,516 - +0.2 2,397,535 + +0.3 2,397,660 - +0.3 2,397,768 + +0.0 2,397,781 - +0.1 2,397,789 + -0.1 2,397,790 - -0.5 2,397,811 + -0.0 2,397,839 + -1.1 2,397,865 - +0.4 2,397,929 + +0.6 2,397,930 - -0.0 2,397,930 - +0.6 2,397,944 + +0.2 2,397,949 + +0.3 2,397,950 - -0.3 2,397,970 + +0.9 2,397,971 - +0.1 2,397,984 - +1.2 2,398,012 - +0.6 2,398,030 + +0.6 2,398,031 - -0.0 2,398,087 - +0.6 2,398,087 - +0.4 2,398,089 + +0.0 2,398,089 + +1.0 2,398,172 + +0.9 2,398,204 - -0.7 2,398,208 + -0.1 2,398,209 - +0.4 2,398,301 - CA265_RS09725 0.12 -0.2 2,398,400 + CA265_RS09725 0.15 -0.6 2,398,401 - CA265_RS09725 0.15 +0.6 2,398,535 - -0.1 2,398,596 + CA265_RS09725 0.21 +1.8 2,398,596 + CA265_RS09725 0.21 +0.4 2,398,597 - CA265_RS09725 0.21 +0.3 2,398,600 + CA265_RS09725 0.21 +0.0 2,398,601 - CA265_RS09725 0.21 -0.5 2,398,626 - CA265_RS09725 0.22 +0.1 2,398,656 + CA265_RS09725 0.23 +1.0 2,398,745 + CA265_RS09725 0.26 +0.9 2,398,745 + CA265_RS09725 0.26 -0.4 2,398,745 + CA265_RS09725 0.26 -0.1 2,398,745 + CA265_RS09725 0.26 +0.3 2,398,756 + CA265_RS09725 0.26 -0.3 2,398,757 - CA265_RS09725 0.26 -0.8 2,398,757 - CA265_RS09725 0.26 +0.3 2,398,823 + CA265_RS09725 0.28 +0.6 2,398,823 + CA265_RS09725 0.28 -0.1 2,398,861 - CA265_RS09725 0.29 +0.8 2,398,871 + CA265_RS09725 0.30 +0.7 2,398,904 - CA265_RS09725 0.31 -1.0 2,398,946 - CA265_RS09725 0.32 -0.0 2,398,989 - CA265_RS09725 0.33 +0.2 2,398,991 - CA265_RS09725 0.33 -0.0 2,399,019 - CA265_RS09725 0.34 -0.8 2,399,037 - CA265_RS09725 0.35 +0.0 2,399,072 + CA265_RS09725 0.36 -0.3 2,399,093 + CA265_RS09725 0.36 +0.7 2,399,093 + CA265_RS09725 0.36 +0.8 2,399,093 + CA265_RS09725 0.36 -0.6 2,399,218 + CA265_RS09725 0.40 +0.8 2,399,218 + CA265_RS09725 0.40 +1.0 2,399,219 - CA265_RS09725 0.40 +0.1 2,399,219 - CA265_RS09725 0.40 +0.1 2,399,219 - CA265_RS09725 0.40 +0.2 2,399,219 - CA265_RS09725 0.40 +0.1 2,399,255 - CA265_RS09725 0.42 -0.9 2,399,256 + CA265_RS09725 0.42 +0.4 2,399,256 + CA265_RS09725 0.42 +0.4 2,399,315 + CA265_RS09725 0.43 +0.5 2,399,316 - CA265_RS09725 0.43 +0.3 2,399,338 + CA265_RS09725 0.44 +0.3 2,399,343 - CA265_RS09725 0.44 -0.3 2,399,351 - CA265_RS09725 0.45 +0.6 2,399,353 + CA265_RS09725 0.45 -0.2 2,399,354 - CA265_RS09725 0.45 +0.2 2,399,405 - CA265_RS09725 0.46 +0.6 2,399,432 - CA265_RS09725 0.47 +1.5 2,399,462 + CA265_RS09725 0.48 +0.3 2,399,500 + CA265_RS09725 0.49 +0.7 2,399,503 - CA265_RS09725 0.49 +0.1 2,399,525 + CA265_RS09725 0.50 +0.6 2,399,603 + CA265_RS09725 0.52 -0.1 2,399,613 - CA265_RS09725 0.53 -0.0 2,399,617 + CA265_RS09725 0.53 +1.2 2,399,618 - CA265_RS09725 0.53 +0.5 2,399,618 - CA265_RS09725 0.53 +1.2 2,399,619 + CA265_RS09725 0.53 +0.1 2,399,653 - CA265_RS09725 0.54 +0.2 2,399,653 - CA265_RS09725 0.54 -0.5 2,399,749 + CA265_RS09725 0.57 +0.5 2,399,750 - CA265_RS09725 0.57 -0.4 2,399,822 - CA265_RS09725 0.59 +0.3 2,399,826 + CA265_RS09725 0.59 +0.1 2,399,826 + CA265_RS09725 0.59 +0.4 2,399,963 + CA265_RS09725 0.64 +0.8 2,400,056 + CA265_RS09725 0.67 +0.7 2,400,063 + CA265_RS09725 0.67 -0.9 2,400,068 - CA265_RS09725 0.67 -0.9 2,400,078 - CA265_RS09725 0.67 +0.5 2,400,106 + CA265_RS09725 0.68 +0.6 2,400,107 - CA265_RS09725 0.68 -0.6 2,400,107 - CA265_RS09725 0.68 +1.1 2,400,107 - CA265_RS09725 0.68 +0.7 2,400,165 - CA265_RS09725 0.70 +0.3 2,400,168 - CA265_RS09725 0.70 +0.3 2,400,168 - CA265_RS09725 0.70 +0.5 2,400,260 + CA265_RS09725 0.73 +0.0 2,400,260 + CA265_RS09725 0.73 +0.4 2,400,261 - CA265_RS09725 0.73 -0.3 2,400,288 + CA265_RS09725 0.74 +0.4 2,400,309 + CA265_RS09725 0.75 +0.2 2,400,309 + CA265_RS09725 0.75 -0.5 2,400,339 - CA265_RS09725 0.76 +0.3 2,400,419 + CA265_RS09725 0.78 +0.2 2,400,420 - CA265_RS09725 0.78 +0.3 2,400,458 + CA265_RS09725 0.79 -0.2 2,400,458 + CA265_RS09725 0.79 +0.1 2,400,459 - CA265_RS09725 0.79 +1.3 2,400,469 + CA265_RS09725 0.80 +0.2 2,400,495 + CA265_RS09725 0.80 +0.1 2,400,570 + CA265_RS09725 0.83 +0.8 2,400,571 - CA265_RS09725 0.83 +0.7 2,400,602 + CA265_RS09725 0.84 +0.7 2,400,618 + CA265_RS09725 0.84 +0.7 2,400,619 - CA265_RS09725 0.84 +0.5 2,400,634 - CA265_RS09725 0.85 +0.6 2,400,675 - CA265_RS09725 0.86 +0.5 2,400,675 - CA265_RS09725 0.86 +0.8 2,400,708 - CA265_RS09725 0.87 +0.2 2,400,715 + CA265_RS09725 0.87 +0.3 2,400,773 + CA265_RS09725 0.89 +0.6 2,400,791 - CA265_RS09725 0.90 -0.0 2,400,818 - +0.1 2,400,890 + +0.4 2,400,890 + +0.6 2,400,999 - -1.5 2,400,999 - +0.8 2,401,000 + +0.7 2,401,001 - +0.4 2,401,003 - +0.9 2,401,045 - +0.8 2,401,045 - +1.0 2,401,047 + +1.2 2,401,212 - +0.2 2,401,227 + +0.2 2,401,264 - CA265_RS09730 0.13 +0.8 2,401,312 + CA265_RS09730 0.19 +0.1 2,401,317 - CA265_RS09730 0.19 -0.1 2,401,317 - CA265_RS09730 0.19 -0.3 2,401,339 - CA265_RS09730 0.22 +0.7 2,401,339 - CA265_RS09730 0.22 +1.0 2,401,413 + CA265_RS09730 0.31 +0.5 2,401,436 + CA265_RS09730 0.33 -1.7 2,401,436 + CA265_RS09730 0.33 -0.1 2,401,502 + CA265_RS09730 0.41 -0.7 2,401,504 + CA265_RS09730 0.41 +0.7 2,401,504 + CA265_RS09730 0.41 -0.1 2,401,505 - CA265_RS09730 0.41 -0.7 2,401,555 + CA265_RS09730 0.47 -2.1 2,401,577 + CA265_RS09730 0.50 -0.5 2,401,635 - CA265_RS09730 0.57 -0.3 2,401,645 - CA265_RS09730 0.58 -0.5 2,401,645 - CA265_RS09730 0.58 -0.1 2,401,650 - CA265_RS09730 0.58 +0.6 2,401,674 + CA265_RS09730 0.61 -0.0 2,401,696 - CA265_RS09730 0.64 +0.8 2,401,698 - CA265_RS09730 0.64 +0.1 2,401,698 - CA265_RS09730 0.64 +1.2 2,401,702 - CA265_RS09730 0.64 -0.2 2,401,747 - CA265_RS09730 0.70 +0.0 2,401,758 - CA265_RS09730 0.71 +0.1 2,401,785 - CA265_RS09730 0.74 +0.2 2,401,792 + CA265_RS09730 0.75 -0.3 2,401,813 + CA265_RS09730 0.77 -0.5 2,401,816 - CA265_RS09730 0.78 +0.4 2,401,896 + CA265_RS09730 0.87 +0.0 2,401,942 - +0.5 2,402,014 - +0.6 2,402,039 + -0.3
Or see this region's nucleotide sequence