Experiment: Sisomicin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_722 and Ac3H11_723 are separated by 15 nucleotides Ac3H11_723 and Ac3H11_724 are separated by 79 nucleotides Ac3H11_724 and Ac3H11_725 are separated by 133 nucleotides
Ac3H11_722 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 11,708 to 13,429
_722
Ac3H11_723 - Cell division protein FtsN, at 13,445 to 14,107
_723
Ac3H11_724 - Periplasmic thiol:disulfide interchange protein DsbA, at 14,187 to 14,834
_724
Ac3H11_725 - LptA, protein essential for LPS transport across the periplasm, at 14,968 to 15,624
_725
Position (kb) 13
14
15 Strain fitness (log2 ratio) -1
0
1 at 13.427 kb on + strand at 13.427 kb on + strand at 13.428 kb on - strand at 13.428 kb on - strand at 13.428 kb on - strand at 13.438 kb on + strand at 13.438 kb on + strand at 13.880 kb on + strand, within Ac3H11_723 at 13.880 kb on + strand, within Ac3H11_723 at 13.880 kb on + strand, within Ac3H11_723 at 13.881 kb on - strand, within Ac3H11_723 at 13.881 kb on - strand, within Ac3H11_723 at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0005 mg/ml remove 13,427 + -0.9 13,427 + -0.8 13,428 - +1.1 13,428 - +0.3 13,428 - -0.4 13,438 + +0.1 13,438 + +0.6 13,880 + Ac3H11_723 0.66 +0.9 13,880 + Ac3H11_723 0.66 +1.0 13,880 + Ac3H11_723 0.66 -1.4 13,881 - Ac3H11_723 0.66 +0.7 13,881 - Ac3H11_723 0.66 +1.6 14,171 + +0.4 14,171 + +0.1 14,171 + +0.8 14,171 + -0.0 14,833 - -0.2 14,833 - -0.2 14,833 - -0.3 14,833 - -0.2 14,833 - -1.1 14,941 + -1.0 14,941 + -0.1 14,941 + +0.1 14,941 + -0.8 14,942 - +0.3 14,942 - +0.6 14,942 - +0.1 14,942 - +0.8
Or see this region's nucleotide sequence