Experiment: Sisomicin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4427 and Ac3H11_4428 overlap by 4 nucleotides Ac3H11_4428 and Ac3H11_4429 are separated by 94 nucleotides Ac3H11_4429 and Ac3H11_4430 are separated by 213 nucleotides Ac3H11_4430 and Ac3H11_4431 are separated by 19 nucleotides
Ac3H11_4427 - Probable transmembrane protein, at 4,120 to 5,073
_4427
Ac3H11_4428 - Molybdopterin-binding protein, at 5,070 to 5,891
_4428
Ac3H11_4429 - Glutamine synthetase type I (EC 6.3.1.2), at 5,986 to 7,479
_4429
Ac3H11_4430 - FIG01198927: hypothetical protein, at 7,693 to 8,205
_4430
Ac3H11_4431 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 8,225 to 9,313
_4431
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5.034 kb on + strand at 5.034 kb on + strand at 5.035 kb on - strand at 5.187 kb on + strand, within Ac3H11_4428 at 5.187 kb on + strand, within Ac3H11_4428 at 5.187 kb on + strand, within Ac3H11_4428 at 5.196 kb on + strand, within Ac3H11_4428 at 5.196 kb on + strand, within Ac3H11_4428 at 5.196 kb on + strand, within Ac3H11_4428 at 5.196 kb on + strand, within Ac3H11_4428 at 5.197 kb on - strand, within Ac3H11_4428 at 5.197 kb on - strand, within Ac3H11_4428 at 5.197 kb on - strand, within Ac3H11_4428 at 5.392 kb on + strand, within Ac3H11_4428 at 5.392 kb on + strand, within Ac3H11_4428 at 5.392 kb on + strand, within Ac3H11_4428 at 5.392 kb on + strand, within Ac3H11_4428 at 5.393 kb on - strand, within Ac3H11_4428 at 5.415 kb on + strand, within Ac3H11_4428 at 5.415 kb on + strand, within Ac3H11_4428 at 5.416 kb on - strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.496 kb on + strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.497 kb on - strand, within Ac3H11_4428 at 5.610 kb on + strand, within Ac3H11_4428 at 5.611 kb on - strand, within Ac3H11_4428 at 5.624 kb on + strand, within Ac3H11_4428 at 5.661 kb on + strand, within Ac3H11_4428 at 5.662 kb on - strand, within Ac3H11_4428 at 5.662 kb on - strand, within Ac3H11_4428 at 5.763 kb on + strand, within Ac3H11_4428 at 5.763 kb on + strand, within Ac3H11_4428 at 5.763 kb on + strand, within Ac3H11_4428 at 5.763 kb on + strand, within Ac3H11_4428 at 5.764 kb on - strand, within Ac3H11_4428 at 5.764 kb on - strand, within Ac3H11_4428 at 5.774 kb on + strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.775 kb on - strand, within Ac3H11_4428 at 5.956 kb on + strand at 5.956 kb on + strand at 5.957 kb on - strand at 5.976 kb on + strand at 5.976 kb on + strand at 5.976 kb on + strand at 5.976 kb on + strand at 5.977 kb on - strand at 5.977 kb on - strand at 5.977 kb on - strand at 5.984 kb on + strand at 6.015 kb on + strand at 6.015 kb on + strand at 7.477 kb on + strand at 7.477 kb on + strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.598 kb on + strand at 7.599 kb on - strand at 7.654 kb on + strand at 7.654 kb on + strand at 7.655 kb on - strand at 7.765 kb on + strand, within Ac3H11_4430 at 7.766 kb on - strand, within Ac3H11_4430 at 7.783 kb on + strand, within Ac3H11_4430 at 7.783 kb on + strand, within Ac3H11_4430 at 7.783 kb on + strand, within Ac3H11_4430 at 7.783 kb on + strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 7.784 kb on - strand, within Ac3H11_4430 at 8.047 kb on + strand, within Ac3H11_4430 at 8.047 kb on + strand, within Ac3H11_4430 at 8.047 kb on + strand, within Ac3H11_4430 at 8.047 kb on + strand, within Ac3H11_4430 at 8.048 kb on - strand, within Ac3H11_4430 at 8.048 kb on - strand, within Ac3H11_4430 at 8.048 kb on - strand, within Ac3H11_4430 at 8.048 kb on - strand, within Ac3H11_4430 at 8.048 kb on - strand, within Ac3H11_4430 at 8.101 kb on + strand, within Ac3H11_4430 at 8.101 kb on + strand, within Ac3H11_4430 at 8.102 kb on - strand, within Ac3H11_4430 at 8.203 kb on + strand at 8.203 kb on + strand at 8.203 kb on + strand at 8.203 kb on + strand at 8.203 kb on + strand at 8.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0005 mg/ml remove 5,034 + +0.8 5,034 + +0.5 5,035 - -0.4 5,187 + Ac3H11_4428 0.14 -0.1 5,187 + Ac3H11_4428 0.14 +0.7 5,187 + Ac3H11_4428 0.14 -1.0 5,196 + Ac3H11_4428 0.15 -0.8 5,196 + Ac3H11_4428 0.15 +0.8 5,196 + Ac3H11_4428 0.15 -0.6 5,196 + Ac3H11_4428 0.15 +1.0 5,197 - Ac3H11_4428 0.15 +0.8 5,197 - Ac3H11_4428 0.15 -0.5 5,197 - Ac3H11_4428 0.15 -0.9 5,392 + Ac3H11_4428 0.39 +0.7 5,392 + Ac3H11_4428 0.39 +0.2 5,392 + Ac3H11_4428 0.39 +0.9 5,392 + Ac3H11_4428 0.39 -0.1 5,393 - Ac3H11_4428 0.39 +0.5 5,415 + Ac3H11_4428 0.42 +0.2 5,415 + Ac3H11_4428 0.42 +0.1 5,416 - Ac3H11_4428 0.42 +1.4 5,496 + Ac3H11_4428 0.52 -1.6 5,496 + Ac3H11_4428 0.52 -0.9 5,496 + Ac3H11_4428 0.52 -0.4 5,496 + Ac3H11_4428 0.52 -0.8 5,496 + Ac3H11_4428 0.52 -0.3 5,496 + Ac3H11_4428 0.52 -1.0 5,496 + Ac3H11_4428 0.52 -0.9 5,496 + Ac3H11_4428 0.52 +0.1 5,496 + Ac3H11_4428 0.52 -0.5 5,497 - Ac3H11_4428 0.52 +0.4 5,497 - Ac3H11_4428 0.52 -0.0 5,497 - Ac3H11_4428 0.52 +0.1 5,497 - Ac3H11_4428 0.52 +0.2 5,497 - Ac3H11_4428 0.52 +0.2 5,497 - Ac3H11_4428 0.52 +0.3 5,610 + Ac3H11_4428 0.66 -0.4 5,611 - Ac3H11_4428 0.66 +0.8 5,624 + Ac3H11_4428 0.67 +0.5 5,661 + Ac3H11_4428 0.72 -0.2 5,662 - Ac3H11_4428 0.72 +0.8 5,662 - Ac3H11_4428 0.72 -0.9 5,763 + Ac3H11_4428 0.84 +1.4 5,763 + Ac3H11_4428 0.84 -0.3 5,763 + Ac3H11_4428 0.84 +0.6 5,763 + Ac3H11_4428 0.84 -1.2 5,764 - Ac3H11_4428 0.84 -0.3 5,764 - Ac3H11_4428 0.84 +0.3 5,774 + Ac3H11_4428 0.86 +0.9 5,775 - Ac3H11_4428 0.86 -0.8 5,775 - Ac3H11_4428 0.86 +0.0 5,775 - Ac3H11_4428 0.86 +0.6 5,775 - Ac3H11_4428 0.86 +1.6 5,775 - Ac3H11_4428 0.86 +0.3 5,775 - Ac3H11_4428 0.86 -0.2 5,775 - Ac3H11_4428 0.86 +1.0 5,956 + +0.7 5,956 + -0.1 5,957 - -0.2 5,976 + -0.0 5,976 + -1.1 5,976 + -0.7 5,976 + +1.0 5,977 - -0.1 5,977 - -2.3 5,977 - -1.2 5,984 + +0.5 6,015 + -0.4 6,015 + +0.2 7,477 + +0.7 7,477 + +0.6 7,478 - +0.9 7,478 - -0.1 7,478 - +0.3 7,478 - -0.5 7,478 - +0.6 7,598 + +0.7 7,599 - +0.9 7,654 + +0.2 7,654 + +0.5 7,655 - -0.3 7,765 + Ac3H11_4430 0.14 +1.3 7,766 - Ac3H11_4430 0.14 +1.9 7,783 + Ac3H11_4430 0.18 +1.2 7,783 + Ac3H11_4430 0.18 +1.7 7,783 + Ac3H11_4430 0.18 +2.2 7,783 + Ac3H11_4430 0.18 +1.1 7,784 - Ac3H11_4430 0.18 -0.3 7,784 - Ac3H11_4430 0.18 +0.4 7,784 - Ac3H11_4430 0.18 +1.0 7,784 - Ac3H11_4430 0.18 +1.2 7,784 - Ac3H11_4430 0.18 +1.2 7,784 - Ac3H11_4430 0.18 +1.5 8,047 + Ac3H11_4430 0.69 +1.4 8,047 + Ac3H11_4430 0.69 +1.4 8,047 + Ac3H11_4430 0.69 +1.6 8,047 + Ac3H11_4430 0.69 +1.2 8,048 - Ac3H11_4430 0.69 -0.8 8,048 - Ac3H11_4430 0.69 +1.2 8,048 - Ac3H11_4430 0.69 +1.1 8,048 - Ac3H11_4430 0.69 +0.7 8,048 - Ac3H11_4430 0.69 +2.0 8,101 + Ac3H11_4430 0.80 +1.4 8,101 + Ac3H11_4430 0.80 +2.1 8,102 - Ac3H11_4430 0.80 +0.4 8,203 + +0.2 8,203 + -0.6 8,203 + -0.1 8,203 + +0.4 8,203 + +0.2 8,204 - -0.3
Or see this region's nucleotide sequence