Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09140 and MMJJ_RS09145 are separated by 288 nucleotides MMJJ_RS09145 and MMJJ_RS09150 are separated by 153 nucleotides
MMJJ_RS09140: MMJJ_RS09140 - carbamoyl-phosphate synthase large subunit, at 1,692,453 to 1,695,698
_RS09140
MMJJ_RS09145: MMJJ_RS09145 - exodeoxyribonuclease III, at 1,695,987 to 1,696,736
_RS09145
MMJJ_RS09150: MMJJ_RS09150 - glutamate--tRNA ligase, at 1,696,890 to 1,698,557
_RS09150
Position (kb)
1695
1696
1697 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1695.761 kb on - strand at 1695.761 kb on - strand at 1695.763 kb on - strand at 1695.763 kb on - strand at 1695.764 kb on + strand at 1695.764 kb on + strand at 1695.783 kb on - strand at 1695.783 kb on - strand at 1695.784 kb on + strand at 1695.784 kb on + strand at 1695.784 kb on + strand at 1695.799 kb on - strand at 1695.800 kb on + strand at 1695.803 kb on - strand at 1695.822 kb on + strand at 1695.851 kb on + strand at 1695.851 kb on - strand at 1695.860 kb on - strand at 1695.860 kb on - strand at 1695.888 kb on + strand at 1695.895 kb on - strand at 1695.899 kb on + strand at 1695.950 kb on + strand at 1695.950 kb on + strand at 1695.950 kb on + strand at 1695.955 kb on + strand at 1695.960 kb on + strand at 1695.975 kb on - strand at 1695.975 kb on - strand at 1695.976 kb on + strand at 1695.981 kb on - strand at 1695.982 kb on + strand at 1695.982 kb on + strand at 1695.986 kb on - strand at 1695.987 kb on + strand at 1696.009 kb on - strand at 1696.009 kb on - strand at 1696.009 kb on - strand at 1696.010 kb on + strand at 1696.010 kb on + strand at 1696.010 kb on + strand at 1696.084 kb on - strand, within MMJJ_RS09145 at 1696.084 kb on - strand, within MMJJ_RS09145 at 1696.084 kb on - strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.085 kb on + strand, within MMJJ_RS09145 at 1696.209 kb on + strand, within MMJJ_RS09145 at 1696.214 kb on - strand, within MMJJ_RS09145 at 1696.216 kb on - strand, within MMJJ_RS09145 at 1696.217 kb on + strand, within MMJJ_RS09145 at 1696.259 kb on + strand, within MMJJ_RS09145 at 1696.262 kb on - strand, within MMJJ_RS09145 at 1696.263 kb on + strand, within MMJJ_RS09145 at 1696.289 kb on + strand, within MMJJ_RS09145 at 1696.299 kb on - strand, within MMJJ_RS09145 at 1696.406 kb on + strand, within MMJJ_RS09145 at 1696.464 kb on + strand, within MMJJ_RS09145 at 1696.464 kb on + strand, within MMJJ_RS09145 at 1696.469 kb on + strand, within MMJJ_RS09145 at 1696.515 kb on + strand, within MMJJ_RS09145 at 1696.616 kb on + strand, within MMJJ_RS09145 at 1696.679 kb on - strand at 1696.682 kb on + strand at 1696.724 kb on - strand at 1696.725 kb on + strand at 1696.725 kb on + strand at 1696.726 kb on - strand at 1696.726 kb on - strand at 1696.734 kb on - strand at 1696.734 kb on - strand at 1696.734 kb on - strand at 1696.735 kb on + strand at 1696.760 kb on - strand at 1696.761 kb on + strand at 1696.782 kb on + strand at 1696.782 kb on + strand at 1696.789 kb on - strand at 1696.789 kb on - strand at 1696.790 kb on + strand at 1696.795 kb on - strand at 1696.796 kb on + strand at 1696.841 kb on + strand at 1696.847 kb on + strand at 1697.300 kb on - strand, within MMJJ_RS09150 at 1697.301 kb on + strand, within MMJJ_RS09150 at 1697.516 kb on - strand, within MMJJ_RS09150
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,695,761 - +0.1 1,695,761 - -0.1 1,695,763 - -2.2 1,695,763 - +0.2 1,695,764 + +1.6 1,695,764 + -0.2 1,695,783 - +0.8 1,695,783 - +0.1 1,695,784 + +0.9 1,695,784 + -0.2 1,695,784 + -0.6 1,695,799 - +1.6 1,695,800 + +0.3 1,695,803 - +1.1 1,695,822 + +0.1 1,695,851 + +0.3 1,695,851 - -0.6 1,695,860 - +1.1 1,695,860 - -0.0 1,695,888 + -0.0 1,695,895 - -0.1 1,695,899 + +0.5 1,695,950 + +0.0 1,695,950 + +0.2 1,695,950 + +0.2 1,695,955 + +0.4 1,695,960 + -0.1 1,695,975 - -0.3 1,695,975 - +0.0 1,695,976 + +0.5 1,695,981 - +0.1 1,695,982 + +1.7 1,695,982 + +0.6 1,695,986 - +0.2 1,695,987 + -0.6 1,696,009 - -0.2 1,696,009 - -0.0 1,696,009 - -0.5 1,696,010 + +0.4 1,696,010 + +0.0 1,696,010 + +0.0 1,696,084 - MMJJ_RS09145 0.13 -0.2 1,696,084 - MMJJ_RS09145 0.13 -0.4 1,696,084 - MMJJ_RS09145 0.13 -1.8 1,696,085 + MMJJ_RS09145 0.13 +1.1 1,696,085 + -1.0 1,696,085 + MMJJ_RS09145 0.13 +1.0 1,696,085 + MMJJ_RS09145 0.13 +0.3 1,696,085 + MMJJ_RS09145 0.13 -0.3 1,696,085 + MMJJ_RS09145 0.13 +0.3 1,696,085 + MMJJ_RS09145 0.13 +0.1 1,696,085 + MMJJ_RS09145 0.13 -0.2 1,696,209 + MMJJ_RS09145 0.30 +0.3 1,696,214 - MMJJ_RS09145 0.30 +0.5 1,696,216 - MMJJ_RS09145 0.31 -0.0 1,696,217 + MMJJ_RS09145 0.31 +0.0 1,696,259 + MMJJ_RS09145 0.36 -0.4 1,696,262 - MMJJ_RS09145 0.37 +0.4 1,696,263 + MMJJ_RS09145 0.37 -0.3 1,696,289 + MMJJ_RS09145 0.40 +0.3 1,696,299 - MMJJ_RS09145 0.42 +0.1 1,696,406 + MMJJ_RS09145 0.56 -1.0 1,696,464 + MMJJ_RS09145 0.64 -0.7 1,696,464 + MMJJ_RS09145 0.64 -0.6 1,696,469 + MMJJ_RS09145 0.64 +1.8 1,696,515 + MMJJ_RS09145 0.70 +0.7 1,696,616 + MMJJ_RS09145 0.84 +1.4 1,696,679 - -0.0 1,696,682 + -0.5 1,696,724 - +0.0 1,696,725 + +0.3 1,696,725 + -0.5 1,696,726 - +0.8 1,696,726 - -0.6 1,696,734 - -0.3 1,696,734 - +0.2 1,696,734 - -0.9 1,696,735 + -0.5 1,696,760 - +0.6 1,696,761 + +0.1 1,696,782 + +0.5 1,696,782 + +0.2 1,696,789 - -0.2 1,696,789 - +0.6 1,696,790 + +2.0 1,696,795 - +0.4 1,696,796 + -0.8 1,696,841 + -3.2 1,696,847 + -0.8 1,697,300 - MMJJ_RS09150 0.25 -2.0 1,697,301 + MMJJ_RS09150 0.25 -2.8 1,697,516 - MMJJ_RS09150 0.38 -2.3
Or see this region's nucleotide sequence