Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09055 and MMJJ_RS09060 are separated by 296 nucleotides MMJJ_RS09060 and MMJJ_RS09065 are separated by 230 nucleotides MMJJ_RS09065 and MMJJ_RS09070 are separated by 332 nucleotides MMJJ_RS09070 and MMJJ_RS09075 are separated by 223 nucleotides
MMJJ_RS09055: MMJJ_RS09055 - NFACT family protein, at 1,672,258 to 1,674,300
_RS09055
MMJJ_RS09060: MMJJ_RS09060 - hypothetical protein, at 1,674,597 to 1,674,824
_RS09060
MMJJ_RS09065: MMJJ_RS09065 - hypothetical protein, at 1,675,055 to 1,675,606
_RS09065
MMJJ_RS09070: MMJJ_RS09070 - hypothetical protein, at 1,675,939 to 1,676,124
_RS09070
MMJJ_RS09075: MMJJ_RS09075 - arginine--tRNA ligase, at 1,676,348 to 1,678,048
_RS09075
Position (kb)
1675
1676 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1674.113 kb on + strand at 1674.117 kb on + strand at 1674.117 kb on + strand at 1674.173 kb on + strand at 1674.346 kb on - strand at 1674.346 kb on - strand at 1674.347 kb on + strand at 1674.347 kb on + strand at 1674.350 kb on - strand at 1674.350 kb on - strand at 1674.355 kb on + strand at 1674.355 kb on + strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.362 kb on + strand at 1674.362 kb on + strand at 1674.370 kb on - strand at 1674.370 kb on - strand at 1674.379 kb on + strand at 1674.381 kb on - strand at 1674.381 kb on - strand at 1674.389 kb on - strand at 1674.389 kb on - strand at 1674.389 kb on - strand at 1674.390 kb on + strand at 1674.390 kb on + strand at 1674.390 kb on + strand at 1674.401 kb on - strand at 1674.410 kb on + strand at 1674.417 kb on - strand at 1674.418 kb on + strand at 1674.419 kb on - strand at 1674.419 kb on - strand at 1674.420 kb on + strand at 1674.420 kb on + strand at 1674.420 kb on + strand at 1674.422 kb on + strand at 1674.431 kb on + strand at 1674.432 kb on - strand at 1674.433 kb on + strand at 1674.433 kb on + strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.436 kb on + strand at 1674.436 kb on + strand at 1674.436 kb on + strand at 1674.440 kb on - strand at 1674.441 kb on + strand at 1674.441 kb on + strand at 1674.442 kb on - strand at 1674.442 kb on - strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.447 kb on + strand at 1674.447 kb on + strand at 1674.447 kb on + strand at 1674.487 kb on - strand at 1674.492 kb on + strand at 1674.492 kb on + strand at 1674.521 kb on + strand at 1674.537 kb on - strand at 1674.540 kb on + strand at 1674.547 kb on + strand at 1674.547 kb on + strand at 1674.592 kb on - strand at 1674.593 kb on + strand at 1674.605 kb on - strand at 1674.631 kb on + strand, within MMJJ_RS09060 at 1674.643 kb on + strand, within MMJJ_RS09060 at 1674.657 kb on - strand, within MMJJ_RS09060 at 1674.657 kb on - strand, within MMJJ_RS09060 at 1674.678 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.732 kb on - strand, within MMJJ_RS09060 at 1674.754 kb on + strand, within MMJJ_RS09060 at 1674.775 kb on - strand, within MMJJ_RS09060 at 1674.827 kb on - strand at 1674.828 kb on + strand at 1674.867 kb on + strand at 1674.877 kb on + strand at 1674.878 kb on - strand at 1674.917 kb on - strand at 1674.957 kb on + strand at 1674.983 kb on - strand at 1674.983 kb on - strand at 1674.983 kb on - strand at 1674.984 kb on + strand at 1674.985 kb on - strand at 1674.986 kb on + strand at 1674.997 kb on - strand at 1674.997 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.002 kb on + strand at 1675.002 kb on + strand at 1675.002 kb on + strand at 1675.017 kb on - strand at 1675.017 kb on - strand at 1675.021 kb on - strand at 1675.021 kb on - strand at 1675.081 kb on - strand at 1675.098 kb on + strand at 1675.145 kb on + strand, within MMJJ_RS09065 at 1675.153 kb on + strand, within MMJJ_RS09065 at 1675.153 kb on + strand, within MMJJ_RS09065 at 1675.159 kb on - strand, within MMJJ_RS09065 at 1675.235 kb on + strand, within MMJJ_RS09065 at 1675.235 kb on + strand, within MMJJ_RS09065 at 1675.253 kb on - strand, within MMJJ_RS09065 at 1675.357 kb on - strand, within MMJJ_RS09065 at 1675.359 kb on - strand, within MMJJ_RS09065 at 1675.359 kb on - strand, within MMJJ_RS09065 at 1675.383 kb on - strand, within MMJJ_RS09065 at 1675.384 kb on + strand, within MMJJ_RS09065 at 1675.384 kb on + strand, within MMJJ_RS09065 at 1675.384 kb on + strand, within MMJJ_RS09065 at 1675.384 kb on + strand, within MMJJ_RS09065 at 1675.412 kb on - strand, within MMJJ_RS09065 at 1675.412 kb on - strand, within MMJJ_RS09065 at 1675.473 kb on - strand, within MMJJ_RS09065 at 1675.531 kb on + strand, within MMJJ_RS09065 at 1675.547 kb on + strand, within MMJJ_RS09065 at 1675.585 kb on + strand at 1675.619 kb on - strand at 1675.619 kb on - strand at 1675.620 kb on + strand at 1675.621 kb on - strand at 1675.622 kb on + strand at 1675.649 kb on - strand at 1675.652 kb on + strand at 1675.652 kb on + strand at 1675.652 kb on + strand at 1675.652 kb on + strand at 1675.653 kb on - strand at 1675.653 kb on - strand at 1675.653 kb on - strand at 1675.654 kb on + strand at 1675.654 kb on + strand at 1675.668 kb on - strand at 1675.668 kb on - strand at 1675.674 kb on - strand at 1675.674 kb on - strand at 1675.674 kb on - strand at 1675.674 kb on - strand at 1675.675 kb on + strand at 1675.675 kb on + strand at 1675.675 kb on + strand at 1675.687 kb on - strand at 1675.687 kb on - strand at 1675.687 kb on - strand at 1675.687 kb on - strand at 1675.688 kb on + strand at 1675.688 kb on + strand at 1675.688 kb on + strand at 1675.688 kb on + strand at 1675.694 kb on - strand at 1675.702 kb on + strand at 1675.703 kb on - strand at 1675.704 kb on + strand at 1675.704 kb on + strand at 1675.704 kb on + strand at 1675.704 kb on + strand at 1675.712 kb on + strand at 1675.712 kb on + strand at 1675.756 kb on - strand at 1675.756 kb on - strand at 1675.756 kb on - strand at 1675.756 kb on - strand at 1675.757 kb on + strand at 1675.764 kb on + strand at 1675.778 kb on + strand at 1675.778 kb on + strand at 1675.809 kb on + strand at 1675.820 kb on - strand at 1675.820 kb on - strand at 1675.820 kb on - strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.821 kb on + strand at 1675.898 kb on - strand at 1675.898 kb on - strand at 1675.898 kb on - strand at 1675.899 kb on + strand at 1675.900 kb on - strand at 1675.903 kb on - strand at 1675.903 kb on - strand at 1675.903 kb on - strand at 1675.903 kb on - strand at 1675.904 kb on + strand at 1675.904 kb on + strand at 1675.904 kb on + strand at 1675.979 kb on - strand, within MMJJ_RS09070 at 1675.979 kb on - strand, within MMJJ_RS09070 at 1675.979 kb on - strand, within MMJJ_RS09070 at 1675.980 kb on + strand, within MMJJ_RS09070 at 1675.980 kb on + strand, within MMJJ_RS09070 at 1675.981 kb on - strand, within MMJJ_RS09070 at 1675.981 kb on - strand, within MMJJ_RS09070 at 1675.994 kb on + strand, within MMJJ_RS09070 at 1676.020 kb on - strand, within MMJJ_RS09070 at 1676.020 kb on - strand, within MMJJ_RS09070 at 1676.021 kb on + strand, within MMJJ_RS09070 at 1676.021 kb on + strand, within MMJJ_RS09070 at 1676.022 kb on - strand, within MMJJ_RS09070 at 1676.022 kb on - strand, within MMJJ_RS09070 at 1676.023 kb on + strand, within MMJJ_RS09070 at 1676.023 kb on + strand, within MMJJ_RS09070 at 1676.116 kb on + strand at 1676.159 kb on - strand at 1676.160 kb on + strand at 1676.160 kb on + strand at 1676.160 kb on + strand at 1676.165 kb on + strand at 1676.168 kb on - strand at 1676.179 kb on + strand at 1676.180 kb on - strand at 1676.181 kb on + strand at 1676.201 kb on + strand at 1676.205 kb on + strand at 1676.213 kb on + strand at 1676.215 kb on - strand at 1676.216 kb on + strand at 1676.220 kb on + strand at 1676.220 kb on + strand at 1676.220 kb on + strand at 1676.229 kb on - strand at 1676.236 kb on + strand at 1676.236 kb on + strand at 1676.239 kb on + strand at 1676.246 kb on + strand at 1676.261 kb on - strand at 1676.265 kb on - strand at 1676.265 kb on - strand at 1676.265 kb on - strand at 1676.266 kb on + strand at 1676.266 kb on + strand at 1676.266 kb on + strand at 1676.266 kb on + strand at 1676.266 kb on + strand at 1676.267 kb on - strand at 1676.267 kb on - strand at 1676.268 kb on + strand at 1676.268 kb on + strand at 1676.268 kb on + strand at 1676.271 kb on - strand at 1676.272 kb on + strand at 1676.272 kb on + strand at 1676.604 kb on + strand, within MMJJ_RS09075
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,674,113 + -3.5 1,674,117 + -1.9 1,674,117 + -2.6 1,674,173 + -2.6 1,674,346 - -0.5 1,674,346 - -0.2 1,674,347 + +0.0 1,674,347 + -0.8 1,674,350 - -0.5 1,674,350 - -0.8 1,674,355 + +1.4 1,674,355 + -0.2 1,674,361 - -0.0 1,674,361 - +0.0 1,674,361 - -0.3 1,674,361 - +0.0 1,674,362 + -0.9 1,674,362 + -0.3 1,674,370 - +0.1 1,674,370 - -2.3 1,674,379 + -0.4 1,674,381 - -1.3 1,674,381 - -0.3 1,674,389 - -0.1 1,674,389 - -0.0 1,674,389 - +0.0 1,674,390 + -0.0 1,674,390 + -0.6 1,674,390 + -0.2 1,674,401 - -0.6 1,674,410 + -0.1 1,674,417 - +0.3 1,674,418 + -0.0 1,674,419 - -0.1 1,674,419 - +2.0 1,674,420 + -0.2 1,674,420 + +0.5 1,674,420 + -0.2 1,674,422 + -0.0 1,674,431 + +0.2 1,674,432 - -0.1 1,674,433 + -0.1 1,674,433 + -0.9 1,674,435 - -0.3 1,674,435 - +0.1 1,674,435 - +0.2 1,674,435 - -0.1 1,674,435 - -0.6 1,674,436 + +0.2 1,674,436 + -0.0 1,674,436 + -0.1 1,674,440 - -0.4 1,674,441 + +0.5 1,674,441 + -0.9 1,674,442 - +0.2 1,674,442 - +2.8 1,674,443 + +0.2 1,674,443 + +0.3 1,674,443 + -0.9 1,674,443 + -0.6 1,674,443 + +0.2 1,674,446 - -0.7 1,674,446 - +0.5 1,674,446 - +0.1 1,674,446 - -0.3 1,674,446 - -1.0 1,674,446 - -0.4 1,674,446 - +0.6 1,674,447 + +0.2 1,674,447 + +0.2 1,674,447 + -0.1 1,674,487 - +0.5 1,674,492 + +0.1 1,674,492 + -0.6 1,674,521 + +0.7 1,674,537 - -0.6 1,674,540 + +0.9 1,674,547 + +0.5 1,674,547 + +0.0 1,674,592 - -0.8 1,674,593 + -0.3 1,674,605 - -0.5 1,674,631 + MMJJ_RS09060 0.15 -0.0 1,674,643 + MMJJ_RS09060 0.20 +0.3 1,674,657 - MMJJ_RS09060 0.26 -0.6 1,674,657 - MMJJ_RS09060 0.26 +0.3 1,674,678 - MMJJ_RS09060 0.36 -0.3 1,674,687 - MMJJ_RS09060 0.39 +0.5 1,674,687 - MMJJ_RS09060 0.39 +0.3 1,674,687 - MMJJ_RS09060 0.39 +0.2 1,674,688 + MMJJ_RS09060 0.40 +0.3 1,674,688 + MMJJ_RS09060 0.40 +0.3 1,674,688 + MMJJ_RS09060 0.40 +0.3 1,674,689 - MMJJ_RS09060 0.40 -1.3 1,674,689 - MMJJ_RS09060 0.40 -0.2 1,674,689 - MMJJ_RS09060 0.40 +0.2 1,674,689 - MMJJ_RS09060 0.40 -1.6 1,674,689 - MMJJ_RS09060 0.40 +0.2 1,674,690 + MMJJ_RS09060 0.41 -0.5 1,674,690 + MMJJ_RS09060 0.41 -0.3 1,674,690 + MMJJ_RS09060 0.41 +0.0 1,674,690 + MMJJ_RS09060 0.41 +0.6 1,674,690 + MMJJ_RS09060 0.41 +0.4 1,674,690 + MMJJ_RS09060 0.41 +0.1 1,674,690 + MMJJ_RS09060 0.41 -0.1 1,674,691 - MMJJ_RS09060 0.41 -0.2 1,674,691 - MMJJ_RS09060 0.41 -0.4 1,674,691 - MMJJ_RS09060 0.41 +0.8 1,674,692 + MMJJ_RS09060 0.42 -2.0 1,674,692 + MMJJ_RS09060 0.42 +0.0 1,674,692 + MMJJ_RS09060 0.42 +0.8 1,674,732 - MMJJ_RS09060 0.59 +0.2 1,674,754 + MMJJ_RS09060 0.69 +0.3 1,674,775 - MMJJ_RS09060 0.78 +0.5 1,674,827 - -0.1 1,674,828 + +0.2 1,674,867 + +1.0 1,674,877 + +0.0 1,674,878 - +0.6 1,674,917 - -0.3 1,674,957 + +0.0 1,674,983 - +0.2 1,674,983 - +0.1 1,674,983 - -0.2 1,674,984 + -0.6 1,674,985 - +0.7 1,674,986 + +0.2 1,674,997 - +0.5 1,674,997 - +0.0 1,675,001 - +0.4 1,675,001 - -0.7 1,675,001 - +0.5 1,675,001 - -0.5 1,675,001 - -0.5 1,675,001 - +0.1 1,675,002 + -0.1 1,675,002 + -1.0 1,675,002 + +0.5 1,675,017 - -0.7 1,675,017 - +0.4 1,675,021 - -0.2 1,675,021 - -0.3 1,675,081 - -0.0 1,675,098 + +0.2 1,675,145 + MMJJ_RS09065 0.16 -0.4 1,675,153 + MMJJ_RS09065 0.18 +0.2 1,675,153 + MMJJ_RS09065 0.18 -0.2 1,675,159 - MMJJ_RS09065 0.19 +0.1 1,675,235 + MMJJ_RS09065 0.33 +0.2 1,675,235 + MMJJ_RS09065 0.33 -0.4 1,675,253 - MMJJ_RS09065 0.36 -0.2 1,675,357 - MMJJ_RS09065 0.55 -0.6 1,675,359 - MMJJ_RS09065 0.55 +0.3 1,675,359 - MMJJ_RS09065 0.55 +0.1 1,675,383 - MMJJ_RS09065 0.59 +3.1 1,675,384 + MMJJ_RS09065 0.60 +0.9 1,675,384 + MMJJ_RS09065 0.60 +0.6 1,675,384 + MMJJ_RS09065 0.60 -0.1 1,675,384 + MMJJ_RS09065 0.60 +0.2 1,675,412 - MMJJ_RS09065 0.65 +0.6 1,675,412 - MMJJ_RS09065 0.65 -0.3 1,675,473 - MMJJ_RS09065 0.76 +0.6 1,675,531 + MMJJ_RS09065 0.86 +0.7 1,675,547 + MMJJ_RS09065 0.89 -0.5 1,675,585 + +0.1 1,675,619 - +0.1 1,675,619 - +1.1 1,675,620 + -0.0 1,675,621 - +0.2 1,675,622 + +0.0 1,675,649 - -0.0 1,675,652 + -0.0 1,675,652 + -0.2 1,675,652 + -0.4 1,675,652 + +0.5 1,675,653 - +0.7 1,675,653 - -1.0 1,675,653 - +2.0 1,675,654 + +0.1 1,675,654 + +0.1 1,675,668 - +0.1 1,675,668 - +0.1 1,675,674 - +0.7 1,675,674 - +0.1 1,675,674 - +0.1 1,675,674 - +0.4 1,675,675 + -0.3 1,675,675 + -0.6 1,675,675 + +0.8 1,675,687 - +0.2 1,675,687 - -1.1 1,675,687 - -1.8 1,675,687 - -0.6 1,675,688 + +0.5 1,675,688 + +3.5 1,675,688 + +0.6 1,675,688 + -0.1 1,675,694 - -0.2 1,675,702 + +0.2 1,675,703 - +0.3 1,675,704 + -0.3 1,675,704 + -0.3 1,675,704 + -0.0 1,675,704 + -0.6 1,675,712 + -0.6 1,675,712 + -2.0 1,675,756 - -0.0 1,675,756 - -0.1 1,675,756 - +0.5 1,675,756 - -0.6 1,675,757 + +0.1 1,675,764 + +0.1 1,675,778 + +0.2 1,675,778 + +0.1 1,675,809 + +0.5 1,675,820 - -1.0 1,675,820 - -0.7 1,675,820 - -0.1 1,675,821 + +0.1 1,675,821 + +0.7 1,675,821 + -0.9 1,675,821 + +0.4 1,675,821 + +0.3 1,675,821 + +1.0 1,675,821 + +0.6 1,675,821 + -0.2 1,675,821 + -0.3 1,675,821 + +0.1 1,675,821 + +0.1 1,675,821 + +0.3 1,675,898 - -0.0 1,675,898 - -0.6 1,675,898 - +0.7 1,675,899 + -0.9 1,675,900 - -0.2 1,675,903 - +0.6 1,675,903 - -0.1 1,675,903 - +0.6 1,675,903 - -0.1 1,675,904 + +0.2 1,675,904 + +0.5 1,675,904 + -0.7 1,675,979 - MMJJ_RS09070 0.22 -0.4 1,675,979 - MMJJ_RS09070 0.22 +0.4 1,675,979 - MMJJ_RS09070 0.22 +0.0 1,675,980 + MMJJ_RS09070 0.22 -0.0 1,675,980 + MMJJ_RS09070 0.22 -0.2 1,675,981 - MMJJ_RS09070 0.23 -0.1 1,675,981 - MMJJ_RS09070 0.23 +0.7 1,675,994 + MMJJ_RS09070 0.30 +0.0 1,676,020 - MMJJ_RS09070 0.44 -0.2 1,676,020 - MMJJ_RS09070 0.44 -0.2 1,676,021 + MMJJ_RS09070 0.44 +0.1 1,676,021 + MMJJ_RS09070 0.44 +0.7 1,676,022 - MMJJ_RS09070 0.45 +0.5 1,676,022 - MMJJ_RS09070 0.45 -1.3 1,676,023 + MMJJ_RS09070 0.45 -0.5 1,676,023 + MMJJ_RS09070 0.45 +0.2 1,676,116 + +0.3 1,676,159 - +1.6 1,676,160 + +0.1 1,676,160 + +0.1 1,676,160 + -0.2 1,676,165 + -0.6 1,676,168 - +1.2 1,676,179 + -0.7 1,676,180 - -0.0 1,676,181 + +1.0 1,676,201 + +0.3 1,676,205 + +0.3 1,676,213 + -0.3 1,676,215 - +0.3 1,676,216 + -0.2 1,676,220 + +0.0 1,676,220 + -0.3 1,676,220 + +1.5 1,676,229 - +0.1 1,676,236 + -0.2 1,676,236 + +0.5 1,676,239 + -1.6 1,676,246 + -0.6 1,676,261 - -0.7 1,676,265 - -0.2 1,676,265 - +0.4 1,676,265 - -0.4 1,676,266 + +1.2 1,676,266 + -0.5 1,676,266 + -0.3 1,676,266 + +0.2 1,676,266 + +0.5 1,676,267 - +0.0 1,676,267 - +0.1 1,676,268 + +0.3 1,676,268 + +2.0 1,676,268 + -0.7 1,676,271 - -2.0 1,676,272 + -1.4 1,676,272 + -1.6 1,676,604 + MMJJ_RS09075 0.15 -2.3
Or see this region's nucleotide sequence