Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09010 and MMJJ_RS09015 are separated by 77 nucleotides MMJJ_RS09015 and MMJJ_RS09020 are separated by 36 nucleotides MMJJ_RS09020 and MMJJ_RS09025 are separated by 71 nucleotides MMJJ_RS09025 and MMJJ_RS09030 are separated by 21 nucleotides
MMJJ_RS09010: MMJJ_RS09010 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,662,823 to 1,663,302
_RS09010
MMJJ_RS09015: MMJJ_RS09015 - SPFH domain-containing protein, at 1,663,380 to 1,664,186
_RS09015
MMJJ_RS09020: MMJJ_RS09020 - hypothetical protein, at 1,664,223 to 1,664,636
_RS09020
MMJJ_RS09025: MMJJ_RS09025 - dTMP kinase, at 1,664,708 to 1,665,307
_RS09025
MMJJ_RS09030: MMJJ_RS09030 - hypothetical protein, at 1,665,329 to 1,668,259
_RS09030
Position (kb)
1664
1665 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1663.235 kb on - strand, within MMJJ_RS09010 at 1663.242 kb on - strand, within MMJJ_RS09010 at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.259 kb on - strand at 1663.259 kb on - strand at 1663.271 kb on + strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.302 kb on - strand at 1663.304 kb on - strand at 1663.329 kb on + strand at 1663.378 kb on + strand at 1663.464 kb on - strand, within MMJJ_RS09015 at 1663.683 kb on + strand, within MMJJ_RS09015 at 1663.767 kb on - strand, within MMJJ_RS09015 at 1663.819 kb on + strand, within MMJJ_RS09015 at 1663.830 kb on - strand, within MMJJ_RS09015 at 1663.860 kb on - strand, within MMJJ_RS09015 at 1663.862 kb on - strand, within MMJJ_RS09015 at 1663.863 kb on + strand, within MMJJ_RS09015 at 1663.906 kb on + strand, within MMJJ_RS09015 at 1663.925 kb on + strand, within MMJJ_RS09015 at 1663.964 kb on - strand, within MMJJ_RS09015 at 1663.993 kb on + strand, within MMJJ_RS09015 at 1663.998 kb on + strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.022 kb on - strand, within MMJJ_RS09015 at 1664.023 kb on + strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.049 kb on + strand, within MMJJ_RS09015 at 1664.091 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.094 kb on + strand, within MMJJ_RS09015 at 1664.133 kb on - strand at 1664.134 kb on + strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.137 kb on + strand at 1664.139 kb on - strand at 1664.142 kb on - strand at 1664.143 kb on + strand at 1664.155 kb on + strand at 1664.156 kb on - strand at 1664.157 kb on + strand at 1664.171 kb on - strand at 1664.186 kb on - strand at 1664.186 kb on - strand at 1664.187 kb on + strand at 1664.187 kb on + strand at 1664.198 kb on - strand at 1664.211 kb on - strand at 1664.218 kb on - strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.220 kb on - strand at 1664.221 kb on + strand at 1664.232 kb on - strand at 1664.232 kb on - strand at 1664.248 kb on + strand at 1664.254 kb on + strand at 1664.254 kb on + strand at 1664.265 kb on + strand, within MMJJ_RS09020 at 1664.268 kb on - strand, within MMJJ_RS09020 at 1664.269 kb on + strand, within MMJJ_RS09020 at 1664.334 kb on - strand, within MMJJ_RS09020 at 1664.338 kb on + strand, within MMJJ_RS09020 at 1664.458 kb on - strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.470 kb on + strand, within MMJJ_RS09020 at 1664.482 kb on + strand, within MMJJ_RS09020 at 1664.530 kb on - strand, within MMJJ_RS09020 at 1664.564 kb on - strand, within MMJJ_RS09020 at 1664.610 kb on - strand at 1664.612 kb on - strand at 1664.613 kb on + strand at 1664.619 kb on - strand at 1664.620 kb on + strand at 1664.621 kb on - strand at 1664.622 kb on + strand at 1664.622 kb on + strand at 1664.623 kb on - strand at 1664.623 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.665 kb on - strand at 1664.665 kb on - strand at 1664.666 kb on + strand at 1664.667 kb on - strand at 1664.667 kb on - strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.676 kb on + strand at 1664.679 kb on - strand at 1664.680 kb on + strand at 1664.691 kb on - strand at 1664.700 kb on - strand at 1664.701 kb on + strand at 1664.702 kb on - strand at 1664.703 kb on + strand at 1664.703 kb on + strand at 1664.704 kb on - strand at 1664.704 kb on - strand at 1664.705 kb on + strand at 1664.709 kb on - strand at 1664.714 kb on - strand at 1664.717 kb on + strand at 1664.719 kb on - strand at 1665.157 kb on - strand at 1665.327 kb on + strand at 1665.355 kb on + strand at 1665.389 kb on - strand at 1665.390 kb on + strand at 1665.433 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.482 kb on + strand at 1665.494 kb on - strand at 1665.497 kb on - strand at 1665.501 kb on + strand at 1665.521 kb on - strand at 1665.587 kb on - strand at 1665.623 kb on - strand, within MMJJ_RS09030
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,663,235 - MMJJ_RS09010 0.86 +0.1 1,663,242 - MMJJ_RS09010 0.87 +0.0 1,663,255 - +0.8 1,663,255 - +0.1 1,663,255 - -0.0 1,663,255 - +0.6 1,663,255 - +2.5 1,663,255 - +0.1 1,663,256 + +0.6 1,663,256 + -0.0 1,663,256 + +0.1 1,663,259 - +0.1 1,663,259 - +0.6 1,663,271 + +0.5 1,663,294 - -0.0 1,663,294 - +1.5 1,663,294 - +0.3 1,663,302 - -0.4 1,663,304 - -0.3 1,663,329 + -2.3 1,663,378 + +0.8 1,663,464 - MMJJ_RS09015 0.10 -0.0 1,663,683 + MMJJ_RS09015 0.38 -0.2 1,663,767 - MMJJ_RS09015 0.48 -0.4 1,663,819 + MMJJ_RS09015 0.54 -0.2 1,663,830 - MMJJ_RS09015 0.56 -0.1 1,663,860 - MMJJ_RS09015 0.59 -0.0 1,663,862 - MMJJ_RS09015 0.60 +0.0 1,663,863 + MMJJ_RS09015 0.60 +0.2 1,663,906 + MMJJ_RS09015 0.65 -0.2 1,663,925 + MMJJ_RS09015 0.68 +0.1 1,663,964 - MMJJ_RS09015 0.72 -0.3 1,663,993 + MMJJ_RS09015 0.76 -0.2 1,663,998 + MMJJ_RS09015 0.77 +0.2 1,664,004 - MMJJ_RS09015 0.77 +2.3 1,664,004 - MMJJ_RS09015 0.77 -0.7 1,664,004 - MMJJ_RS09015 0.77 +0.3 1,664,004 - MMJJ_RS09015 0.77 -0.5 1,664,004 - MMJJ_RS09015 0.77 -0.7 1,664,004 - MMJJ_RS09015 0.77 -0.3 1,664,005 + MMJJ_RS09015 0.77 -1.0 1,664,005 + MMJJ_RS09015 0.77 +0.7 1,664,005 + MMJJ_RS09015 0.77 +0.4 1,664,010 - MMJJ_RS09015 0.78 +0.2 1,664,010 - MMJJ_RS09015 0.78 -0.5 1,664,010 - MMJJ_RS09015 0.78 -0.5 1,664,016 - MMJJ_RS09015 0.79 +0.6 1,664,016 - MMJJ_RS09015 0.79 +0.6 1,664,016 - MMJJ_RS09015 0.79 -0.1 1,664,022 - MMJJ_RS09015 0.80 +1.0 1,664,023 + MMJJ_RS09015 0.80 -0.9 1,664,040 - MMJJ_RS09015 0.82 +2.3 1,664,040 - MMJJ_RS09015 0.82 -0.3 1,664,049 + MMJJ_RS09015 0.83 -0.2 1,664,091 - MMJJ_RS09015 0.88 +0.9 1,664,093 - MMJJ_RS09015 0.88 +0.1 1,664,093 - MMJJ_RS09015 0.88 +0.1 1,664,094 + MMJJ_RS09015 0.88 -0.4 1,664,133 - +0.1 1,664,134 + +0.2 1,664,136 - +0.7 1,664,136 - -0.2 1,664,136 - +0.3 1,664,137 + +0.5 1,664,139 - +0.1 1,664,142 - +0.2 1,664,143 + -0.6 1,664,155 + -1.2 1,664,156 - -0.3 1,664,157 + -1.1 1,664,171 - +0.1 1,664,186 - -0.7 1,664,186 - +0.5 1,664,187 + -0.0 1,664,187 + +0.1 1,664,198 - -0.2 1,664,211 - +0.0 1,664,218 - +0.1 1,664,219 + -1.0 1,664,219 + +0.7 1,664,219 + +0.3 1,664,220 - -0.0 1,664,221 + +0.2 1,664,232 - -0.9 1,664,232 - -0.5 1,664,248 + +2.0 1,664,254 + -0.2 1,664,254 + -0.1 1,664,265 + MMJJ_RS09020 0.10 +1.0 1,664,268 - MMJJ_RS09020 0.11 -0.2 1,664,269 + MMJJ_RS09020 0.11 -0.0 1,664,334 - MMJJ_RS09020 0.27 +0.0 1,664,338 + MMJJ_RS09020 0.28 +0.0 1,664,458 - MMJJ_RS09020 0.57 +4.1 1,664,459 + MMJJ_RS09020 0.57 +1.6 1,664,459 + MMJJ_RS09020 0.57 -0.7 1,664,459 + MMJJ_RS09020 0.57 +1.2 1,664,470 + MMJJ_RS09020 0.60 +0.3 1,664,482 + MMJJ_RS09020 0.63 +0.4 1,664,530 - MMJJ_RS09020 0.74 -0.0 1,664,564 - MMJJ_RS09020 0.82 +0.2 1,664,610 - -0.2 1,664,612 - +0.2 1,664,613 + -0.6 1,664,619 - +0.0 1,664,620 + +0.0 1,664,621 - -0.1 1,664,622 + +0.2 1,664,622 + -0.5 1,664,623 - -0.2 1,664,623 - -0.0 1,664,628 - -0.7 1,664,628 - +1.6 1,664,628 - +0.0 1,664,629 + +0.2 1,664,629 + +0.4 1,664,629 + -0.2 1,664,664 + -2.0 1,664,664 + -0.6 1,664,664 + -0.0 1,664,664 + -2.8 1,664,665 - -0.4 1,664,665 - -0.1 1,664,666 + +0.7 1,664,667 - +0.2 1,664,667 - +0.3 1,664,668 + -0.1 1,664,668 + +0.4 1,664,668 + -0.9 1,664,668 + -0.3 1,664,675 - +2.0 1,664,675 - +2.0 1,664,675 - -0.1 1,664,676 + +0.2 1,664,679 - +1.1 1,664,680 + -0.8 1,664,691 - +0.8 1,664,700 - -0.6 1,664,701 + -0.2 1,664,702 - +0.0 1,664,703 + +0.1 1,664,703 + +0.3 1,664,704 - -0.2 1,664,704 - -0.5 1,664,705 + -0.6 1,664,709 - -0.5 1,664,714 - -3.5 1,664,717 + -0.1 1,664,719 - -1.0 1,665,157 - -1.6 1,665,327 + -2.0 1,665,355 + -2.8 1,665,389 - -0.8 1,665,390 + -1.2 1,665,433 - -0.6 1,665,481 - -1.8 1,665,481 - +1.2 1,665,481 - +1.8 1,665,481 - +0.6 1,665,481 - -0.1 1,665,481 - -1.4 1,665,481 - -0.3 1,665,481 - +0.7 1,665,482 + +1.6 1,665,494 - -1.5 1,665,497 - -1.0 1,665,501 + +0.3 1,665,521 - -1.0 1,665,587 - -0.0 1,665,623 - MMJJ_RS09030 0.10 -1.0
Or see this region's nucleotide sequence