Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07955 and MMJJ_RS07960 are separated by 52 nucleotides MMJJ_RS07960 and MMJJ_RS07965 are separated by 10 nucleotides MMJJ_RS07965 and MMJJ_RS07970 are separated by 5 nucleotides
MMJJ_RS07955: MMJJ_RS07955 - ThiF family adenylyltransferase, at 1,459,261 to 1,459,875
_RS07955
MMJJ_RS07960: MMJJ_RS07960 - quinolinate synthase NadA, at 1,459,928 to 1,460,845
_RS07960
MMJJ_RS07965: MMJJ_RS07965 - hypothetical protein, at 1,460,856 to 1,461,215
_RS07965
MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363
_RS07970
Position (kb) 1460
1461
1462 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1459.936 kb on + strand at 1460.083 kb on + strand, within MMJJ_RS07960 at 1460.212 kb on + strand, within MMJJ_RS07960 at 1460.264 kb on + strand, within MMJJ_RS07960 at 1460.512 kb on + strand, within MMJJ_RS07960 at 1460.740 kb on + strand, within MMJJ_RS07960 at 1460.872 kb on - strand at 1460.873 kb on + strand at 1460.873 kb on + strand at 1460.875 kb on - strand at 1460.917 kb on - strand, within MMJJ_RS07965 at 1460.918 kb on + strand, within MMJJ_RS07965 at 1460.920 kb on - strand, within MMJJ_RS07965 at 1460.950 kb on + strand, within MMJJ_RS07965 at 1460.953 kb on - strand, within MMJJ_RS07965 at 1460.955 kb on - strand, within MMJJ_RS07965 at 1460.956 kb on + strand, within MMJJ_RS07965 at 1460.956 kb on + strand, within MMJJ_RS07965 at 1461.040 kb on - strand, within MMJJ_RS07965 at 1461.051 kb on - strand, within MMJJ_RS07965 at 1461.051 kb on - strand, within MMJJ_RS07965 at 1461.074 kb on + strand, within MMJJ_RS07965 at 1461.207 kb on + strand at 1462.053 kb on + strand, within MMJJ_RS07970 at 1462.093 kb on + strand, within MMJJ_RS07970
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,459,936 + -3.0 1,460,083 + MMJJ_RS07960 0.17 -1.0 1,460,212 + MMJJ_RS07960 0.31 -1.8 1,460,264 + MMJJ_RS07960 0.37 -2.8 1,460,512 + MMJJ_RS07960 0.64 -0.3 1,460,740 + MMJJ_RS07960 0.88 -2.8 1,460,872 - -0.1 1,460,873 + +0.2 1,460,873 + +0.1 1,460,875 - +1.6 1,460,917 - MMJJ_RS07965 0.17 -1.7 1,460,918 + MMJJ_RS07965 0.17 +1.6 1,460,920 - MMJJ_RS07965 0.18 +1.3 1,460,950 + MMJJ_RS07965 0.26 +0.4 1,460,953 - MMJJ_RS07965 0.27 -0.4 1,460,955 - MMJJ_RS07965 0.28 +0.7 1,460,956 + MMJJ_RS07965 0.28 +0.3 1,460,956 + MMJJ_RS07965 0.28 +3.0 1,461,040 - MMJJ_RS07965 0.51 -0.6 1,461,051 - MMJJ_RS07965 0.54 -1.6 1,461,051 - MMJJ_RS07965 0.54 +0.4 1,461,074 + MMJJ_RS07965 0.61 -0.1 1,461,207 + +0.0 1,462,053 + MMJJ_RS07970 0.73 +0.0 1,462,093 + MMJJ_RS07970 0.76 -2.3
Or see this region's nucleotide sequence