Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07725 and MMJJ_RS07730 are separated by 233 nucleotides MMJJ_RS07730 and MMJJ_RS07735 are separated by 18 nucleotides MMJJ_RS07735 and MMJJ_RS07740 are separated by 120 nucleotides MMJJ_RS07740 and MMJJ_RS07745 overlap by 4 nucleotides
MMJJ_RS07725: MMJJ_RS07725 - DNA primase DnaG, at 1,417,365 to 1,418,675
_RS07725
MMJJ_RS07730: MMJJ_RS07730 - tRNA-Gly, at 1,418,909 to 1,418,981
_RS07730
MMJJ_RS07735: MMJJ_RS07735 - tRNA-Gly, at 1,419,000 to 1,419,072
_RS07735
MMJJ_RS07740: MMJJ_RS07740 - DUF2334 domain-containing protein, at 1,419,193 to 1,419,993
_RS07740
MMJJ_RS07745: MMJJ_RS07745 - DUF5814 domain-containing protein, at 1,419,990 to 1,422,425
_RS07745
Position (kb)
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1418.372 kb on - strand, within MMJJ_RS07725 at 1418.645 kb on + strand at 1418.651 kb on - strand at 1418.676 kb on + strand at 1418.737 kb on - strand at 1418.752 kb on - strand at 1418.753 kb on + strand at 1418.753 kb on + strand at 1418.756 kb on - strand at 1418.760 kb on - strand at 1418.760 kb on - strand at 1418.761 kb on + strand at 1418.766 kb on + strand at 1418.766 kb on + strand at 1418.774 kb on - strand at 1418.774 kb on - strand at 1418.775 kb on + strand at 1418.775 kb on + strand at 1418.775 kb on + strand at 1418.776 kb on - strand at 1418.776 kb on - strand at 1418.777 kb on + strand at 1418.777 kb on + strand at 1418.777 kb on + strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.779 kb on + strand at 1418.780 kb on - strand at 1418.780 kb on - strand at 1418.781 kb on + strand at 1418.784 kb on - strand at 1418.784 kb on - strand at 1418.784 kb on - strand at 1418.785 kb on + strand at 1418.785 kb on + strand at 1418.787 kb on - strand at 1418.787 kb on - strand at 1418.787 kb on - strand at 1418.823 kb on - strand at 1419.044 kb on - strand, within MMJJ_RS07735 at 1419.080 kb on + strand at 1419.080 kb on + strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.148 kb on - strand at 1419.149 kb on + strand at 1419.158 kb on - strand at 1419.159 kb on + strand at 1419.162 kb on - strand at 1419.163 kb on + strand at 1419.165 kb on + strand at 1419.175 kb on + strand at 1419.184 kb on - strand at 1419.184 kb on - strand at 1419.185 kb on + strand at 1419.185 kb on + strand at 1419.228 kb on + strand at 1419.319 kb on + strand, within MMJJ_RS07740 at 1419.328 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.360 kb on + strand, within MMJJ_RS07740 at 1419.364 kb on + strand, within MMJJ_RS07740 at 1419.370 kb on - strand, within MMJJ_RS07740 at 1419.371 kb on + strand, within MMJJ_RS07740 at 1419.425 kb on + strand, within MMJJ_RS07740 at 1419.484 kb on - strand, within MMJJ_RS07740 at 1419.485 kb on + strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.613 kb on + strand, within MMJJ_RS07740 at 1419.680 kb on - strand, within MMJJ_RS07740 at 1419.695 kb on + strand, within MMJJ_RS07740 at 1419.727 kb on + strand, within MMJJ_RS07740 at 1419.727 kb on + strand, within MMJJ_RS07740 at 1419.731 kb on + strand, within MMJJ_RS07740 at 1419.732 kb on - strand, within MMJJ_RS07740 at 1419.754 kb on + strand, within MMJJ_RS07740 at 1419.754 kb on + strand, within MMJJ_RS07740 at 1419.791 kb on - strand, within MMJJ_RS07740 at 1419.811 kb on - strand, within MMJJ_RS07740 at 1419.906 kb on - strand, within MMJJ_RS07740 at 1419.951 kb on - strand at 1419.956 kb on + strand at 1420.058 kb on + strand at 1420.092 kb on + strand at 1420.112 kb on + strand at 1420.121 kb on - strand at 1420.121 kb on - strand at 1420.231 kb on + strand at 1420.231 kb on + strand at 1420.232 kb on - strand at 1420.233 kb on + strand at 1420.326 kb on - strand, within MMJJ_RS07745 at 1420.326 kb on - strand, within MMJJ_RS07745 at 1420.329 kb on - strand, within MMJJ_RS07745 at 1420.329 kb on - strand, within MMJJ_RS07745 at 1420.362 kb on + strand, within MMJJ_RS07745 at 1420.409 kb on - strand, within MMJJ_RS07745 at 1420.409 kb on - strand, within MMJJ_RS07745 at 1420.410 kb on + strand, within MMJJ_RS07745 at 1420.411 kb on - strand, within MMJJ_RS07745 at 1420.411 kb on - strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.438 kb on + strand, within MMJJ_RS07745 at 1420.493 kb on - strand, within MMJJ_RS07745 at 1420.498 kb on - strand, within MMJJ_RS07745 at 1420.538 kb on - strand, within MMJJ_RS07745 at 1420.539 kb on + strand, within MMJJ_RS07745 at 1420.590 kb on - strand, within MMJJ_RS07745 at 1420.652 kb on - strand, within MMJJ_RS07745 at 1420.652 kb on - strand, within MMJJ_RS07745 at 1420.653 kb on + strand, within MMJJ_RS07745 at 1420.653 kb on + strand, within MMJJ_RS07745 at 1420.659 kb on + strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.684 kb on + strand, within MMJJ_RS07745 at 1420.684 kb on + strand, within MMJJ_RS07745 at 1420.724 kb on + strand, within MMJJ_RS07745 at 1420.755 kb on - strand, within MMJJ_RS07745 at 1420.804 kb on - strand, within MMJJ_RS07745 at 1420.933 kb on - strand, within MMJJ_RS07745 at 1420.986 kb on - strand, within MMJJ_RS07745 at 1420.986 kb on - strand, within MMJJ_RS07745 at 1420.989 kb on - strand, within MMJJ_RS07745 at 1420.989 kb on - strand, within MMJJ_RS07745
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,418,372 - MMJJ_RS07725 0.77 -0.9 1,418,645 + -3.0 1,418,651 - -2.2 1,418,676 + -1.4 1,418,737 - -2.3 1,418,752 - -1.5 1,418,753 + -3.0 1,418,753 + -1.9 1,418,756 - -0.6 1,418,760 - -1.3 1,418,760 - -1.4 1,418,761 + -3.0 1,418,766 + -0.6 1,418,766 + -0.1 1,418,774 - +0.9 1,418,774 - +0.2 1,418,775 + +0.6 1,418,775 + +0.5 1,418,775 + -0.4 1,418,776 - -0.1 1,418,776 - -1.1 1,418,777 + -0.7 1,418,777 + +0.1 1,418,777 + +0.2 1,418,778 - +0.2 1,418,778 - +0.5 1,418,778 - +0.6 1,418,778 - -0.3 1,418,778 - -0.3 1,418,778 - +0.2 1,418,778 - -0.0 1,418,779 + +0.2 1,418,780 - +0.8 1,418,780 - +2.1 1,418,781 + -0.9 1,418,784 - -1.9 1,418,784 - +0.6 1,418,784 - +0.6 1,418,785 + +0.6 1,418,785 + +0.6 1,418,787 - +0.4 1,418,787 - +0.3 1,418,787 - -0.4 1,418,823 - +0.4 1,419,044 - MMJJ_RS07735 0.60 -3.3 1,419,080 + -2.5 1,419,080 + +0.4 1,419,129 - -0.5 1,419,129 - -1.2 1,419,129 - +0.2 1,419,130 + -0.2 1,419,130 + -0.6 1,419,130 + +0.2 1,419,130 + -1.9 1,419,130 + +0.1 1,419,131 - +0.2 1,419,131 - -0.7 1,419,131 - +0.3 1,419,131 - -0.7 1,419,132 + +0.5 1,419,132 + +0.3 1,419,132 + +0.7 1,419,132 + +0.5 1,419,132 + +0.3 1,419,132 + +0.1 1,419,132 + +0.1 1,419,133 - +0.4 1,419,133 - -0.2 1,419,133 - +0.5 1,419,133 - +1.1 1,419,148 - +0.1 1,419,149 + +0.3 1,419,158 - +0.3 1,419,159 + -0.1 1,419,162 - +0.7 1,419,163 + -0.3 1,419,165 + -0.3 1,419,175 + -0.8 1,419,184 - +1.1 1,419,184 - +0.1 1,419,185 + +0.5 1,419,185 + +1.7 1,419,228 + +0.9 1,419,319 + MMJJ_RS07740 0.16 -0.2 1,419,328 - MMJJ_RS07740 0.17 -0.4 1,419,359 - MMJJ_RS07740 0.21 +0.6 1,419,359 - MMJJ_RS07740 0.21 +2.6 1,419,360 + MMJJ_RS07740 0.21 +1.0 1,419,364 + MMJJ_RS07740 0.21 +0.3 1,419,370 - MMJJ_RS07740 0.22 -0.4 1,419,371 + MMJJ_RS07740 0.22 +0.2 1,419,425 + MMJJ_RS07740 0.29 +0.1 1,419,484 - MMJJ_RS07740 0.36 +0.6 1,419,485 + MMJJ_RS07740 0.36 +0.7 1,419,534 - MMJJ_RS07740 0.43 -0.7 1,419,534 - MMJJ_RS07740 0.43 -0.1 1,419,613 + MMJJ_RS07740 0.52 -0.3 1,419,680 - MMJJ_RS07740 0.61 +0.3 1,419,695 + MMJJ_RS07740 0.63 +0.5 1,419,727 + MMJJ_RS07740 0.67 +0.5 1,419,727 + MMJJ_RS07740 0.67 +0.5 1,419,731 + MMJJ_RS07740 0.67 -0.3 1,419,732 - MMJJ_RS07740 0.67 +0.1 1,419,754 + MMJJ_RS07740 0.70 +0.4 1,419,754 + MMJJ_RS07740 0.70 +0.3 1,419,791 - MMJJ_RS07740 0.75 -0.2 1,419,811 - MMJJ_RS07740 0.77 +0.4 1,419,906 - MMJJ_RS07740 0.89 -0.2 1,419,951 - -0.1 1,419,956 + +0.2 1,420,058 + +0.9 1,420,092 + +0.1 1,420,112 + +0.2 1,420,121 - -1.3 1,420,121 - -0.4 1,420,231 + +0.3 1,420,231 + -0.1 1,420,232 - -3.1 1,420,233 + -0.4 1,420,326 - MMJJ_RS07745 0.14 -0.1 1,420,326 - MMJJ_RS07745 0.14 -0.8 1,420,329 - MMJJ_RS07745 0.14 -0.4 1,420,329 - MMJJ_RS07745 0.14 +0.3 1,420,362 + MMJJ_RS07745 0.15 -0.1 1,420,409 - MMJJ_RS07745 0.17 -0.3 1,420,409 - MMJJ_RS07745 0.17 +0.1 1,420,410 + MMJJ_RS07745 0.17 -0.2 1,420,411 - MMJJ_RS07745 0.17 -0.9 1,420,411 - MMJJ_RS07745 0.17 -0.1 1,420,412 + MMJJ_RS07745 0.17 -0.1 1,420,412 + MMJJ_RS07745 0.17 +0.2 1,420,412 + MMJJ_RS07745 0.17 -0.0 1,420,438 + MMJJ_RS07745 0.18 +0.3 1,420,493 - MMJJ_RS07745 0.21 -1.0 1,420,498 - MMJJ_RS07745 0.21 +0.3 1,420,538 - MMJJ_RS07745 0.22 +1.3 1,420,539 + MMJJ_RS07745 0.23 -0.6 1,420,590 - MMJJ_RS07745 0.25 +1.0 1,420,652 - MMJJ_RS07745 0.27 +0.2 1,420,652 - MMJJ_RS07745 0.27 -0.2 1,420,653 + MMJJ_RS07745 0.27 +1.1 1,420,653 + MMJJ_RS07745 0.27 -0.0 1,420,659 + MMJJ_RS07745 0.27 +1.7 1,420,683 - MMJJ_RS07745 0.28 +0.7 1,420,683 - MMJJ_RS07745 0.28 +0.5 1,420,683 - MMJJ_RS07745 0.28 -0.7 1,420,684 + MMJJ_RS07745 0.28 +1.6 1,420,684 + MMJJ_RS07745 0.28 +0.4 1,420,724 + MMJJ_RS07745 0.30 -0.9 1,420,755 - MMJJ_RS07745 0.31 +0.2 1,420,804 - MMJJ_RS07745 0.33 +2.1 1,420,933 - MMJJ_RS07745 0.39 +0.5 1,420,986 - MMJJ_RS07745 0.41 +0.1 1,420,986 - MMJJ_RS07745 0.41 +0.1 1,420,989 - MMJJ_RS07745 0.41 -0.4 1,420,989 - MMJJ_RS07745 0.41 -0.6
Or see this region's nucleotide sequence