Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05985 and MMJJ_RS05990 are separated by 161 nucleotides MMJJ_RS05990 and MMJJ_RS05995 are separated by 2 nucleotides
MMJJ_RS05985: MMJJ_RS05985 - ABC transporter substrate-binding protein, at 1,113,051 to 1,113,773
_RS05985
MMJJ_RS05990: MMJJ_RS05990 - phosphatase PAP2 family protein, at 1,113,935 to 1,114,483
_RS05990
MMJJ_RS05995: MMJJ_RS05995 - ATP-binding cassette domain-containing protein, at 1,114,486 to 1,116,135
_RS05995
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1112.942 kb on - strand at 1112.943 kb on + strand at 1112.943 kb on + strand at 1112.950 kb on + strand at 1112.957 kb on + strand at 1112.961 kb on - strand at 1112.961 kb on - strand at 1112.977 kb on + strand at 1113.049 kb on + strand at 1113.092 kb on - strand at 1113.120 kb on + strand at 1113.128 kb on - strand, within MMJJ_RS05985 at 1113.148 kb on - strand, within MMJJ_RS05985 at 1113.180 kb on - strand, within MMJJ_RS05985 at 1113.201 kb on + strand, within MMJJ_RS05985 at 1113.259 kb on - strand, within MMJJ_RS05985 at 1113.259 kb on - strand, within MMJJ_RS05985 at 1113.260 kb on + strand, within MMJJ_RS05985 at 1113.260 kb on + strand, within MMJJ_RS05985 at 1113.262 kb on + strand, within MMJJ_RS05985 at 1113.262 kb on + strand, within MMJJ_RS05985 at 1113.281 kb on + strand, within MMJJ_RS05985 at 1113.326 kb on - strand, within MMJJ_RS05985 at 1113.327 kb on + strand, within MMJJ_RS05985 at 1113.327 kb on + strand, within MMJJ_RS05985 at 1113.327 kb on + strand, within MMJJ_RS05985 at 1113.351 kb on - strand, within MMJJ_RS05985 at 1113.352 kb on + strand, within MMJJ_RS05985 at 1113.404 kb on - strand, within MMJJ_RS05985 at 1113.404 kb on - strand, within MMJJ_RS05985 at 1113.405 kb on + strand, within MMJJ_RS05985 at 1113.405 kb on + strand, within MMJJ_RS05985 at 1113.405 kb on + strand, within MMJJ_RS05985 at 1113.405 kb on + strand, within MMJJ_RS05985 at 1113.408 kb on - strand, within MMJJ_RS05985 at 1113.408 kb on - strand, within MMJJ_RS05985 at 1113.420 kb on - strand, within MMJJ_RS05985 at 1113.420 kb on - strand, within MMJJ_RS05985 at 1113.436 kb on - strand, within MMJJ_RS05985 at 1113.437 kb on + strand, within MMJJ_RS05985 at 1113.556 kb on - strand, within MMJJ_RS05985 at 1113.656 kb on + strand, within MMJJ_RS05985 at 1113.695 kb on - strand, within MMJJ_RS05985 at 1113.696 kb on + strand, within MMJJ_RS05985 at 1113.696 kb on + strand, within MMJJ_RS05985 at 1113.704 kb on - strand at 1113.704 kb on - strand at 1113.705 kb on + strand at 1113.719 kb on - strand at 1113.720 kb on + strand at 1113.720 kb on + strand at 1113.720 kb on + strand at 1113.738 kb on - strand at 1113.756 kb on - strand at 1113.823 kb on - strand at 1113.824 kb on + strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.836 kb on - strand at 1113.836 kb on - strand at 1113.837 kb on + strand at 1113.843 kb on - strand at 1113.844 kb on + strand at 1113.844 kb on + strand at 1113.857 kb on - strand at 1113.868 kb on - strand at 1113.869 kb on + strand at 1113.870 kb on - strand at 1113.870 kb on - strand at 1113.887 kb on - strand at 1113.887 kb on - strand at 1113.888 kb on + strand at 1113.888 kb on + strand at 1113.892 kb on + strand at 1113.933 kb on - strand at 1113.940 kb on + strand at 1113.998 kb on + strand, within MMJJ_RS05990 at 1113.998 kb on + strand, within MMJJ_RS05990 at 1114.044 kb on - strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.047 kb on + strand, within MMJJ_RS05990 at 1114.113 kb on - strand, within MMJJ_RS05990 at 1114.132 kb on + strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.143 kb on - strand, within MMJJ_RS05990 at 1114.152 kb on - strand, within MMJJ_RS05990 at 1114.164 kb on - strand, within MMJJ_RS05990 at 1114.216 kb on - strand, within MMJJ_RS05990 at 1114.350 kb on - strand, within MMJJ_RS05990 at 1114.434 kb on - strand at 1114.435 kb on + strand at 1114.435 kb on + strand at 1114.438 kb on + strand at 1114.447 kb on - strand at 1114.456 kb on - strand at 1114.477 kb on - strand at 1114.482 kb on + strand at 1114.482 kb on + strand at 1114.482 kb on + strand at 1114.498 kb on + strand at 1114.580 kb on + strand at 1115.372 kb on + strand, within MMJJ_RS05995 at 1115.444 kb on + strand, within MMJJ_RS05995
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 1,112,942 - +0.4 1,112,943 + +0.2 1,112,943 + +0.2 1,112,950 + +0.2 1,112,957 + +2.4 1,112,961 - -0.3 1,112,961 - -0.4 1,112,977 + +0.1 1,113,049 + +0.0 1,113,092 - -0.5 1,113,120 + +0.8 1,113,128 - MMJJ_RS05985 0.11 +0.0 1,113,148 - MMJJ_RS05985 0.13 +0.3 1,113,180 - MMJJ_RS05985 0.18 +0.4 1,113,201 + MMJJ_RS05985 0.21 +0.7 1,113,259 - MMJJ_RS05985 0.29 +1.9 1,113,259 - MMJJ_RS05985 0.29 +0.4 1,113,260 + MMJJ_RS05985 0.29 -1.2 1,113,260 + MMJJ_RS05985 0.29 +0.2 1,113,262 + MMJJ_RS05985 0.29 -0.1 1,113,262 + MMJJ_RS05985 0.29 +0.5 1,113,281 + MMJJ_RS05985 0.32 -0.2 1,113,326 - MMJJ_RS05985 0.38 -0.1 1,113,327 + MMJJ_RS05985 0.38 +0.2 1,113,327 + MMJJ_RS05985 0.38 +0.1 1,113,327 + MMJJ_RS05985 0.38 -1.1 1,113,351 - MMJJ_RS05985 0.41 +0.3 1,113,352 + MMJJ_RS05985 0.42 -0.2 1,113,404 - MMJJ_RS05985 0.49 -0.6 1,113,404 - MMJJ_RS05985 0.49 -0.1 1,113,405 + MMJJ_RS05985 0.49 +0.2 1,113,405 + MMJJ_RS05985 0.49 +1.1 1,113,405 + MMJJ_RS05985 0.49 +0.1 1,113,405 + MMJJ_RS05985 0.49 +0.5 1,113,408 - MMJJ_RS05985 0.49 -0.1 1,113,408 - MMJJ_RS05985 0.49 +1.8 1,113,420 - MMJJ_RS05985 0.51 +1.3 1,113,420 - MMJJ_RS05985 0.51 +2.0 1,113,436 - MMJJ_RS05985 0.53 -0.1 1,113,437 + MMJJ_RS05985 0.53 +0.2 1,113,556 - MMJJ_RS05985 0.70 +0.9 1,113,656 + MMJJ_RS05985 0.84 +0.8 1,113,695 - MMJJ_RS05985 0.89 +1.8 1,113,696 + MMJJ_RS05985 0.89 +0.1 1,113,696 + MMJJ_RS05985 0.89 +0.6 1,113,704 - -0.8 1,113,704 - -0.6 1,113,705 + -0.4 1,113,719 - +0.4 1,113,720 + +0.5 1,113,720 + +0.7 1,113,720 + +2.8 1,113,738 - +0.1 1,113,756 - -0.3 1,113,823 - +0.2 1,113,824 + +0.2 1,113,825 - +0.5 1,113,825 - -0.0 1,113,825 - +0.4 1,113,825 - -0.1 1,113,825 - -0.5 1,113,825 - -0.0 1,113,825 - +0.3 1,113,826 + +0.0 1,113,826 + -0.3 1,113,826 + +2.3 1,113,826 + +0.9 1,113,826 + -0.2 1,113,826 + +0.5 1,113,826 + +0.4 1,113,826 + +0.6 1,113,826 + +0.6 1,113,826 + +0.3 1,113,836 - +0.0 1,113,836 - -0.2 1,113,837 + -0.3 1,113,843 - +0.2 1,113,844 + -0.5 1,113,844 + +0.1 1,113,857 - +0.3 1,113,868 - +0.1 1,113,869 + -0.1 1,113,870 - -0.3 1,113,870 - -0.4 1,113,887 - +0.5 1,113,887 - +0.2 1,113,888 + +0.0 1,113,888 + +0.5 1,113,892 + +0.7 1,113,933 - +0.4 1,113,940 + +1.1 1,113,998 + MMJJ_RS05990 0.11 +0.6 1,113,998 + MMJJ_RS05990 0.11 +0.0 1,114,044 - MMJJ_RS05990 0.20 -0.9 1,114,045 + MMJJ_RS05990 0.20 +0.1 1,114,045 + MMJJ_RS05990 0.20 -2.4 1,114,045 + MMJJ_RS05990 0.20 +0.8 1,114,046 - MMJJ_RS05990 0.20 +0.2 1,114,046 - MMJJ_RS05990 0.20 +0.2 1,114,046 - MMJJ_RS05990 0.20 +0.8 1,114,046 - MMJJ_RS05990 0.20 +0.7 1,114,046 - MMJJ_RS05990 0.20 +1.2 1,114,046 - MMJJ_RS05990 0.20 +0.1 1,114,046 - MMJJ_RS05990 0.20 +0.4 1,114,046 - MMJJ_RS05990 0.20 +0.9 1,114,047 + MMJJ_RS05990 0.20 -0.3 1,114,113 - MMJJ_RS05990 0.32 -1.4 1,114,132 + MMJJ_RS05990 0.36 +0.4 1,114,140 - MMJJ_RS05990 0.37 -0.3 1,114,140 - MMJJ_RS05990 0.37 -0.1 1,114,140 - MMJJ_RS05990 0.37 -1.4 1,114,141 + MMJJ_RS05990 0.38 +1.0 1,114,141 + MMJJ_RS05990 0.38 -0.0 1,114,141 + MMJJ_RS05990 0.38 +0.1 1,114,143 - MMJJ_RS05990 0.38 +0.2 1,114,152 - MMJJ_RS05990 0.40 +0.4 1,114,164 - MMJJ_RS05990 0.42 +0.4 1,114,216 - MMJJ_RS05990 0.51 +0.2 1,114,350 - MMJJ_RS05990 0.76 -0.1 1,114,434 - -0.4 1,114,435 + -0.1 1,114,435 + -0.0 1,114,438 + +0.1 1,114,447 - -0.2 1,114,456 - -0.6 1,114,477 - -0.3 1,114,482 + -2.0 1,114,482 + +0.3 1,114,482 + -0.3 1,114,498 + -0.0 1,114,580 + -1.0 1,115,372 + MMJJ_RS05995 0.54 -1.0 1,115,444 + MMJJ_RS05995 0.58 -3.9
Or see this region's nucleotide sequence