Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04775 and MMJJ_RS04780 are separated by 3 nucleotides MMJJ_RS04780 and MMJJ_RS04785 are separated by 12 nucleotides MMJJ_RS04785 and MMJJ_RS04790 overlap by 20 nucleotides
MMJJ_RS04775: MMJJ_RS04775 - DNA-binding protein, at 896,721 to 897,632
_RS04775
MMJJ_RS04780: MMJJ_RS04780 - nucleic acid-binding protein, at 897,636 to 897,953
_RS04780
MMJJ_RS04785: MMJJ_RS04785 - OB-fold nucleic acid binding domain-containing protein, at 897,966 to 898,352
_RS04785
MMJJ_RS04790: MMJJ_RS04790 - biotin--[acetyl-CoA-carboxylase] ligase, at 898,333 to 899,058
_RS04790
Position (kb) 897
898 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 896.638 kb on + strand at 896.686 kb on - strand at 896.686 kb on - strand at 896.706 kb on - strand at 896.750 kb on - strand at 896.751 kb on + strand at 896.751 kb on + strand at 896.751 kb on + strand at 896.752 kb on - strand at 896.752 kb on - strand at 896.752 kb on - strand at 896.781 kb on + strand at 896.810 kb on - strand at 896.812 kb on - strand at 896.812 kb on - strand at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.816 kb on - strand, within MMJJ_RS04775 at 896.817 kb on + strand, within MMJJ_RS04775 at 896.817 kb on + strand, within MMJJ_RS04775 at 896.870 kb on - strand, within MMJJ_RS04775 at 896.910 kb on + strand, within MMJJ_RS04775 at 896.921 kb on + strand, within MMJJ_RS04775 at 896.960 kb on + strand, within MMJJ_RS04775 at 897.019 kb on - strand, within MMJJ_RS04775 at 897.067 kb on - strand, within MMJJ_RS04775 at 897.068 kb on + strand, within MMJJ_RS04775 at 897.187 kb on - strand, within MMJJ_RS04775 at 897.188 kb on + strand, within MMJJ_RS04775 at 897.364 kb on - strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.417 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.518 kb on + strand, within MMJJ_RS04775 at 897.520 kb on - strand, within MMJJ_RS04775 at 897.538 kb on - strand, within MMJJ_RS04775 at 897.539 kb on + strand, within MMJJ_RS04775 at 897.540 kb on - strand, within MMJJ_RS04775 at 897.541 kb on + strand at 897.541 kb on + strand at 897.554 kb on + strand at 897.570 kb on + strand at 897.596 kb on - strand at 897.597 kb on + strand at 897.598 kb on - strand at 897.639 kb on - strand at 897.640 kb on + strand at 897.802 kb on - strand, within MMJJ_RS04780 at 897.803 kb on + strand, within MMJJ_RS04780 at 897.806 kb on - strand, within MMJJ_RS04780 at 897.807 kb on + strand, within MMJJ_RS04780 at 897.863 kb on - strand, within MMJJ_RS04780 at 897.864 kb on + strand, within MMJJ_RS04780 at 897.883 kb on - strand, within MMJJ_RS04780 at 897.884 kb on + strand, within MMJJ_RS04780 at 897.884 kb on + strand, within MMJJ_RS04780 at 898.003 kb on - strand at 898.004 kb on + strand at 898.050 kb on - strand, within MMJJ_RS04785 at 898.050 kb on - strand, within MMJJ_RS04785 at 898.050 kb on - strand, within MMJJ_RS04785 at 898.052 kb on - strand, within MMJJ_RS04785 at 898.052 kb on - strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.062 kb on + strand, within MMJJ_RS04785 at 898.093 kb on - strand, within MMJJ_RS04785 at 898.103 kb on + strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.268 kb on + strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.278 kb on + strand, within MMJJ_RS04785 at 898.281 kb on - strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.285 kb on - strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.330 kb on - strand at 898.336 kb on - strand at 898.336 kb on - strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.351 kb on + strand at 898.351 kb on + strand at 898.411 kb on - strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.485 kb on - strand, within MMJJ_RS04790 at 898.602 kb on + strand, within MMJJ_RS04790 at 898.722 kb on + strand at 898.790 kb on - strand, within MMJJ_RS04790 at 898.850 kb on - strand, within MMJJ_RS04790 at 898.851 kb on + strand, within MMJJ_RS04790 at 898.924 kb on + strand at 898.931 kb on - strand, within MMJJ_RS04790
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 896,638 + +0.1 896,686 - -0.6 896,686 - -0.2 896,706 - +1.7 896,750 - -0.6 896,751 + -0.6 896,751 + +0.1 896,751 + -0.5 896,752 - -0.2 896,752 - -0.5 896,752 - +0.4 896,781 + +0.1 896,810 - +0.3 896,812 - +0.2 896,812 - +0.3 896,813 + MMJJ_RS04775 0.10 -0.3 896,813 + MMJJ_RS04775 0.10 +2.0 896,813 + MMJJ_RS04775 0.10 -0.6 896,813 + MMJJ_RS04775 0.10 -0.4 896,813 + MMJJ_RS04775 0.10 +0.2 896,813 + MMJJ_RS04775 0.10 +0.4 896,816 - MMJJ_RS04775 0.10 -0.6 896,817 + MMJJ_RS04775 0.11 -0.5 896,817 + MMJJ_RS04775 0.11 +1.0 896,870 - MMJJ_RS04775 0.16 -0.0 896,910 + MMJJ_RS04775 0.21 +0.2 896,921 + MMJJ_RS04775 0.22 -0.3 896,960 + MMJJ_RS04775 0.26 -0.0 897,019 - MMJJ_RS04775 0.33 +0.1 897,067 - MMJJ_RS04775 0.38 +0.9 897,068 + MMJJ_RS04775 0.38 -0.6 897,187 - MMJJ_RS04775 0.51 -0.7 897,188 + MMJJ_RS04775 0.51 +0.2 897,364 - MMJJ_RS04775 0.71 -0.4 897,413 + MMJJ_RS04775 0.76 -0.5 897,413 + MMJJ_RS04775 0.76 -0.4 897,417 + MMJJ_RS04775 0.76 +1.1 897,423 + MMJJ_RS04775 0.77 +2.7 897,423 + MMJJ_RS04775 0.77 +1.0 897,518 + MMJJ_RS04775 0.87 -0.5 897,520 - MMJJ_RS04775 0.88 +0.3 897,538 - MMJJ_RS04775 0.90 -0.8 897,539 + MMJJ_RS04775 0.90 -0.2 897,540 - MMJJ_RS04775 0.90 +2.8 897,541 + -0.2 897,541 + +2.3 897,554 + -0.4 897,570 + -0.2 897,596 - +0.2 897,597 + +1.1 897,598 - +0.2 897,639 - +0.1 897,640 + +0.1 897,802 - MMJJ_RS04780 0.52 -2.2 897,803 + MMJJ_RS04780 0.53 +0.5 897,806 - MMJJ_RS04780 0.53 +1.1 897,807 + MMJJ_RS04780 0.54 +1.4 897,863 - MMJJ_RS04780 0.71 +0.5 897,864 + MMJJ_RS04780 0.72 +0.2 897,883 - MMJJ_RS04780 0.78 -0.5 897,884 + MMJJ_RS04780 0.78 +0.2 897,884 + MMJJ_RS04780 0.78 -0.5 898,003 - -0.9 898,004 + +0.4 898,050 - MMJJ_RS04785 0.22 -0.3 898,050 - MMJJ_RS04785 0.22 +0.1 898,050 - MMJJ_RS04785 0.22 +0.1 898,052 - MMJJ_RS04785 0.22 +0.1 898,052 - MMJJ_RS04785 0.22 -0.2 898,053 + MMJJ_RS04785 0.22 -0.4 898,053 + MMJJ_RS04785 0.22 +0.1 898,053 + MMJJ_RS04785 0.22 -0.4 898,062 + MMJJ_RS04785 0.25 +0.4 898,093 - MMJJ_RS04785 0.33 -0.1 898,103 + MMJJ_RS04785 0.35 -2.2 898,145 - MMJJ_RS04785 0.46 -1.2 898,145 - MMJJ_RS04785 0.46 -0.8 898,268 + MMJJ_RS04785 0.78 +0.3 898,277 - MMJJ_RS04785 0.80 +0.1 898,277 - MMJJ_RS04785 0.80 -0.2 898,277 - MMJJ_RS04785 0.80 +0.8 898,278 + MMJJ_RS04785 0.81 +0.4 898,281 - MMJJ_RS04785 0.81 -0.1 898,282 + MMJJ_RS04785 0.82 +2.1 898,282 + MMJJ_RS04785 0.82 -1.0 898,282 + MMJJ_RS04785 0.82 +0.6 898,282 + MMJJ_RS04785 0.82 +0.1 898,282 + MMJJ_RS04785 0.82 -0.2 898,282 + MMJJ_RS04785 0.82 +0.7 898,282 + MMJJ_RS04785 0.82 -0.7 898,285 - MMJJ_RS04785 0.82 -0.7 898,286 + MMJJ_RS04785 0.83 -0.4 898,286 + MMJJ_RS04785 0.83 -0.0 898,330 - -0.6 898,336 - -1.2 898,336 - -0.5 898,337 + +1.7 898,337 + +0.2 898,337 + -0.3 898,351 + -2.0 898,351 + -1.6 898,411 - MMJJ_RS04790 0.11 -2.5 898,412 + MMJJ_RS04790 0.11 -1.0 898,412 + MMJJ_RS04790 0.11 -1.4 898,485 - MMJJ_RS04790 0.21 -1.2 898,602 + MMJJ_RS04790 0.37 -2.2 898,722 + -1.8 898,790 - MMJJ_RS04790 0.63 -2.5 898,850 - MMJJ_RS04790 0.71 -2.8 898,851 + MMJJ_RS04790 0.71 -2.2 898,924 + -3.0 898,931 - MMJJ_RS04790 0.82 -2.9
Or see this region's nucleotide sequence