Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04110 and MMJJ_RS04115 are separated by 81 nucleotides MMJJ_RS04115 and MMJJ_RS04120 are separated by 40 nucleotides MMJJ_RS04120 and MMJJ_RS04125 are separated by 28 nucleotides
MMJJ_RS04110: MMJJ_RS04110 - hypothetical protein, at 762,893 to 763,261
_RS04110
MMJJ_RS04115: MMJJ_RS04115 - HDIG domain-containing protein, at 763,343 to 764,029
_RS04115
MMJJ_RS04120: MMJJ_RS04120 - amino acid ABC transporter ATP-binding protein, at 764,070 to 764,801
_RS04120
MMJJ_RS04125: MMJJ_RS04125 - tRNA (guanine(10)-N(2))-dimethyltransferase, at 764,830 to 765,951
_RS04125
Position (kb) 764
765 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 763.104 kb on + strand, within MMJJ_RS04110 at 763.138 kb on + strand, within MMJJ_RS04110 at 763.176 kb on - strand, within MMJJ_RS04110 at 763.192 kb on + strand, within MMJJ_RS04110 at 763.235 kb on - strand at 763.251 kb on + strand at 763.257 kb on + strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.280 kb on - strand at 763.280 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.294 kb on - strand at 763.294 kb on - strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.297 kb on + strand at 763.297 kb on + strand at 763.297 kb on + strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.305 kb on + strand at 763.309 kb on - strand at 763.309 kb on - strand at 763.310 kb on + strand at 763.310 kb on + strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.322 kb on + strand at 763.323 kb on - strand at 763.324 kb on + strand at 763.324 kb on + strand at 763.324 kb on + strand at 763.325 kb on - strand at 763.325 kb on - strand at 763.325 kb on - strand at 763.341 kb on - strand at 763.341 kb on - strand at 763.376 kb on + strand at 763.403 kb on + strand at 763.410 kb on + strand at 763.410 kb on + strand at 763.411 kb on - strand at 763.412 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.459 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.525 kb on - strand, within MMJJ_RS04115 at 763.537 kb on - strand, within MMJJ_RS04115 at 763.594 kb on + strand, within MMJJ_RS04115 at 763.594 kb on + strand, within MMJJ_RS04115 at 763.636 kb on - strand, within MMJJ_RS04115 at 763.659 kb on - strand, within MMJJ_RS04115 at 763.707 kb on - strand, within MMJJ_RS04115 at 763.767 kb on - strand, within MMJJ_RS04115 at 763.797 kb on - strand, within MMJJ_RS04115 at 763.797 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.859 kb on + strand, within MMJJ_RS04115 at 763.867 kb on + strand, within MMJJ_RS04115 at 763.878 kb on - strand, within MMJJ_RS04115 at 763.879 kb on + strand, within MMJJ_RS04115 at 763.894 kb on - strand, within MMJJ_RS04115 at 764.119 kb on - strand at 764.129 kb on + strand at 764.159 kb on + strand, within MMJJ_RS04120 at 764.201 kb on - strand, within MMJJ_RS04120 at 764.240 kb on - strand, within MMJJ_RS04120 at 764.261 kb on - strand, within MMJJ_RS04120 at 764.328 kb on + strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.387 kb on + strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.429 kb on + strand, within MMJJ_RS04120 at 764.431 kb on - strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.594 kb on + strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.644 kb on - strand, within MMJJ_RS04120 at 764.741 kb on - strand at 764.741 kb on - strand at 764.742 kb on + strand at 764.751 kb on + strand at 764.751 kb on + strand at 764.795 kb on - strand at 764.795 kb on - strand at 764.796 kb on + strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.800 kb on + strand at 764.800 kb on + strand at 764.802 kb on - strand at 764.815 kb on + strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.925 kb on - strand at 764.931 kb on - strand at 764.962 kb on + strand, within MMJJ_RS04125 at 764.992 kb on + strand, within MMJJ_RS04125 at 765.230 kb on - strand, within MMJJ_RS04125 at 765.231 kb on + strand, within MMJJ_RS04125 at 765.368 kb on - strand, within MMJJ_RS04125 at 765.486 kb on - strand, within MMJJ_RS04125 at 765.490 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.562 kb on - strand, within MMJJ_RS04125 at 765.563 kb on + strand, within MMJJ_RS04125 at 765.571 kb on - strand, within MMJJ_RS04125 at 765.572 kb on + strand, within MMJJ_RS04125 at 765.576 kb on - strand, within MMJJ_RS04125 at 765.577 kb on + strand, within MMJJ_RS04125 at 765.580 kb on - strand, within MMJJ_RS04125 at 765.604 kb on - strand, within MMJJ_RS04125 at 765.626 kb on + strand, within MMJJ_RS04125 at 765.650 kb on + strand, within MMJJ_RS04125 at 765.691 kb on + strand, within MMJJ_RS04125
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 763,104 + MMJJ_RS04110 0.57 -4.0 763,138 + MMJJ_RS04110 0.66 +0.4 763,176 - MMJJ_RS04110 0.77 -3.3 763,192 + MMJJ_RS04110 0.81 -2.2 763,235 - -2.5 763,251 + -1.2 763,257 + -3.6 763,261 - -1.7 763,261 - -3.5 763,261 - -3.2 763,261 - -1.2 763,261 - -3.5 763,261 - -3.3 763,262 + -1.0 763,262 + -1.4 763,262 + -2.7 763,262 + -3.3 763,262 + -2.3 763,262 + -2.9 763,262 + -1.7 763,262 + -3.5 763,262 + -0.2 763,263 - -3.2 763,263 - -3.9 763,263 - -3.3 763,263 - -4.6 763,264 + -1.3 763,264 + -1.3 763,264 + +0.1 763,264 + -2.0 763,264 + -3.2 763,264 + -1.2 763,280 - +0.6 763,280 - -1.1 763,292 - -0.6 763,292 - +0.4 763,292 - +0.6 763,292 - -2.7 763,292 - -0.2 763,292 - -0.9 763,293 + -0.0 763,293 + -0.0 763,293 + +0.4 763,293 + -0.4 763,294 - +0.2 763,294 - +1.2 763,295 + +0.1 763,295 + -0.2 763,295 + +0.1 763,295 + +0.8 763,297 + -0.5 763,297 + -0.3 763,297 + +0.7 763,304 - +0.3 763,304 - -0.2 763,304 - -0.2 763,304 - +1.1 763,305 + -0.2 763,309 - -0.1 763,309 - +0.1 763,310 + -0.1 763,310 + -0.5 763,321 - -0.5 763,321 - +0.4 763,321 - -0.5 763,321 - +0.0 763,322 + +1.0 763,323 - -0.2 763,324 + -0.4 763,324 + +0.7 763,324 + +0.4 763,325 - +0.2 763,325 - -0.2 763,325 - +0.4 763,341 - +0.4 763,341 - +0.3 763,376 + +0.4 763,403 + +0.0 763,410 + +0.5 763,410 + +0.7 763,411 - +0.6 763,412 + MMJJ_RS04115 0.10 +0.4 763,414 + MMJJ_RS04115 0.10 +0.1 763,414 + MMJJ_RS04115 0.10 -0.4 763,414 + MMJJ_RS04115 0.10 +0.3 763,417 - MMJJ_RS04115 0.11 -0.2 763,417 - MMJJ_RS04115 0.11 -0.2 763,417 - MMJJ_RS04115 0.11 +0.1 763,459 + MMJJ_RS04115 0.17 +0.1 763,499 + MMJJ_RS04115 0.23 +0.2 763,499 + MMJJ_RS04115 0.23 +0.1 763,499 + MMJJ_RS04115 0.23 +0.3 763,499 + MMJJ_RS04115 0.23 +0.3 763,525 - MMJJ_RS04115 0.26 +0.2 763,537 - MMJJ_RS04115 0.28 +0.8 763,594 + MMJJ_RS04115 0.37 -0.8 763,594 + MMJJ_RS04115 0.37 +0.7 763,636 - MMJJ_RS04115 0.43 +0.5 763,659 - MMJJ_RS04115 0.46 +0.6 763,707 - MMJJ_RS04115 0.53 +0.6 763,767 - MMJJ_RS04115 0.62 -0.0 763,797 - MMJJ_RS04115 0.66 +0.1 763,797 - MMJJ_RS04115 0.66 +0.5 763,845 - MMJJ_RS04115 0.73 +0.0 763,845 - MMJJ_RS04115 0.73 -0.1 763,845 - MMJJ_RS04115 0.73 -0.6 763,846 + MMJJ_RS04115 0.73 -0.1 763,846 + MMJJ_RS04115 0.73 -0.5 763,859 + MMJJ_RS04115 0.75 -0.3 763,867 + MMJJ_RS04115 0.76 -0.0 763,878 - MMJJ_RS04115 0.78 +0.1 763,879 + MMJJ_RS04115 0.78 +0.1 763,894 - MMJJ_RS04115 0.80 +0.0 764,119 - +0.1 764,129 + -0.6 764,159 + MMJJ_RS04120 0.12 +0.0 764,201 - MMJJ_RS04120 0.18 +1.2 764,240 - MMJJ_RS04120 0.23 +1.2 764,261 - MMJJ_RS04120 0.26 +0.7 764,328 + MMJJ_RS04120 0.35 +0.1 764,363 - MMJJ_RS04120 0.40 -0.2 764,363 - MMJJ_RS04120 0.40 +0.0 764,363 - MMJJ_RS04120 0.40 -0.4 764,387 + MMJJ_RS04120 0.43 +1.8 764,428 - MMJJ_RS04120 0.49 -0.6 764,428 - MMJJ_RS04120 0.49 +0.1 764,429 + MMJJ_RS04120 0.49 +0.1 764,431 - MMJJ_RS04120 0.49 +0.5 764,546 + MMJJ_RS04120 0.65 -0.8 764,546 + MMJJ_RS04120 0.65 -0.0 764,594 + MMJJ_RS04120 0.72 +0.2 764,602 - MMJJ_RS04120 0.73 +0.5 764,602 - MMJJ_RS04120 0.73 +0.3 764,602 - MMJJ_RS04120 0.73 -0.6 764,644 - MMJJ_RS04120 0.78 +2.6 764,741 - -0.2 764,741 - +0.8 764,742 + +0.0 764,751 + -0.3 764,751 + +0.2 764,795 - +0.2 764,795 - +0.6 764,796 + -0.2 764,799 - -0.3 764,799 - -0.2 764,799 - +0.7 764,800 + +0.4 764,800 + -0.6 764,802 - +0.1 764,815 + +0.1 764,820 - +0.2 764,820 - +0.3 764,820 - +0.2 764,925 - +0.3 764,931 - -0.5 764,962 + MMJJ_RS04125 0.12 -0.3 764,992 + MMJJ_RS04125 0.14 -1.2 765,230 - MMJJ_RS04125 0.36 -0.4 765,231 + MMJJ_RS04125 0.36 +0.0 765,368 - MMJJ_RS04125 0.48 -0.6 765,486 - MMJJ_RS04125 0.58 -0.0 765,490 - MMJJ_RS04125 0.59 -0.3 765,532 - MMJJ_RS04125 0.63 -0.1 765,532 - MMJJ_RS04125 0.63 -1.6 765,532 - MMJJ_RS04125 0.63 -1.9 765,533 + MMJJ_RS04125 0.63 -0.8 765,533 + MMJJ_RS04125 0.63 -0.1 765,562 - MMJJ_RS04125 0.65 -0.4 765,563 + MMJJ_RS04125 0.65 -0.2 765,571 - MMJJ_RS04125 0.66 -0.3 765,572 + MMJJ_RS04125 0.66 +0.5 765,576 - MMJJ_RS04125 0.66 -0.9 765,577 + MMJJ_RS04125 0.67 -1.2 765,580 - MMJJ_RS04125 0.67 -1.5 765,604 - MMJJ_RS04125 0.69 -0.9 765,626 + MMJJ_RS04125 0.71 -0.4 765,650 + MMJJ_RS04125 0.73 -0.1 765,691 + MMJJ_RS04125 0.77 +1.3
Or see this region's nucleotide sequence