Experiment: Formate pooled triplicate planktonic cells
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01500 and MMJJ_RS01505 are separated by 122 nucleotides MMJJ_RS01505 and MMJJ_RS01510 are separated by 95 nucleotides MMJJ_RS01510 and MMJJ_RS01515 are separated by 130 nucleotides MMJJ_RS01515 and MMJJ_RS01520 are separated by 10 nucleotides MMJJ_RS01520 and MMJJ_RS01525 are separated by 14 nucleotides
MMJJ_RS01500: MMJJ_RS01500 - transcriptional regulator, at 294,027 to 295,007
_RS01500
MMJJ_RS01505: MMJJ_RS01505 - DUF2124 domain-containing protein, at 295,130 to 295,606
_RS01505
MMJJ_RS01510: MMJJ_RS01510 - ATP-binding protein, at 295,702 to 297,099
_RS01510
MMJJ_RS01515: MMJJ_RS01515 - DUF2178 domain-containing protein, at 297,230 to 297,616
_RS01515
MMJJ_RS01520: MMJJ_RS01520 - helix-turn-helix transcriptional regulator, at 297,627 to 297,830
_RS01520
MMJJ_RS01525: MMJJ_RS01525 - ketopantoate reductase family protein, at 297,845 to 298,765
_RS01525
Position (kb) 295
296
297
298 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 294.713 kb on - strand, within MMJJ_RS01500 at 294.748 kb on + strand, within MMJJ_RS01500 at 294.889 kb on + strand, within MMJJ_RS01500 at 294.908 kb on - strand, within MMJJ_RS01500 at 294.994 kb on + strand at 295.007 kb on - strand at 295.014 kb on - strand at 295.028 kb on - strand at 295.038 kb on + strand at 295.050 kb on + strand at 295.067 kb on - strand at 295.090 kb on + strand at 295.091 kb on - strand at 295.093 kb on - strand at 295.093 kb on - strand at 295.093 kb on - strand at 295.093 kb on - strand at 295.093 kb on - strand at 295.094 kb on + strand at 295.096 kb on - strand at 295.096 kb on - strand at 295.096 kb on - strand at 295.096 kb on - strand at 295.096 kb on - strand at 295.096 kb on - strand at 295.097 kb on + strand at 295.097 kb on + strand at 295.097 kb on + strand at 295.097 kb on + strand at 295.100 kb on + strand at 295.100 kb on + strand at 295.108 kb on + strand at 295.282 kb on - strand, within MMJJ_RS01505 at 295.295 kb on - strand, within MMJJ_RS01505 at 295.295 kb on - strand, within MMJJ_RS01505 at 295.317 kb on - strand, within MMJJ_RS01505 at 295.366 kb on - strand, within MMJJ_RS01505 at 295.367 kb on + strand, within MMJJ_RS01505 at 295.378 kb on - strand, within MMJJ_RS01505 at 295.378 kb on - strand, within MMJJ_RS01505 at 295.379 kb on + strand, within MMJJ_RS01505 at 295.391 kb on + strand, within MMJJ_RS01505 at 295.444 kb on - strand, within MMJJ_RS01505 at 295.563 kb on - strand at 295.566 kb on - strand at 295.567 kb on + strand at 295.609 kb on - strand at 295.609 kb on - strand at 295.610 kb on + strand at 295.610 kb on + strand at 295.610 kb on + strand at 295.610 kb on + strand at 295.637 kb on + strand at 295.655 kb on - strand at 295.661 kb on + strand at 295.667 kb on - strand at 295.667 kb on - strand at 295.668 kb on + strand at 295.668 kb on + strand at 295.671 kb on - strand at 295.671 kb on - strand at 295.672 kb on + strand at 295.679 kb on - strand at 295.700 kb on - strand at 295.701 kb on + strand at 295.701 kb on + strand at 295.706 kb on - strand at 295.706 kb on - strand at 295.707 kb on + strand at 295.707 kb on + strand at 295.788 kb on - strand at 295.789 kb on + strand at 295.789 kb on + strand at 295.793 kb on - strand at 295.794 kb on + strand at 295.796 kb on + strand at 295.797 kb on - strand at 295.820 kb on - strand at 295.839 kb on - strand at 295.839 kb on - strand at 295.841 kb on - strand at 295.845 kb on + strand, within MMJJ_RS01510 at 295.863 kb on - strand, within MMJJ_RS01510 at 295.920 kb on - strand, within MMJJ_RS01510 at 295.923 kb on + strand, within MMJJ_RS01510 at 295.954 kb on + strand, within MMJJ_RS01510 at 295.966 kb on - strand, within MMJJ_RS01510 at 295.966 kb on - strand, within MMJJ_RS01510 at 295.967 kb on + strand, within MMJJ_RS01510 at 295.967 kb on + strand, within MMJJ_RS01510 at 295.967 kb on + strand, within MMJJ_RS01510 at 295.986 kb on + strand, within MMJJ_RS01510 at 296.001 kb on + strand, within MMJJ_RS01510 at 296.069 kb on - strand, within MMJJ_RS01510 at 296.096 kb on - strand, within MMJJ_RS01510 at 296.097 kb on + strand, within MMJJ_RS01510 at 296.097 kb on + strand, within MMJJ_RS01510 at 296.122 kb on + strand, within MMJJ_RS01510 at 296.217 kb on - strand at 296.248 kb on + strand, within MMJJ_RS01510 at 296.265 kb on - strand at 296.265 kb on - strand, within MMJJ_RS01510 at 296.304 kb on - strand, within MMJJ_RS01510 at 296.337 kb on - strand, within MMJJ_RS01510 at 296.378 kb on + strand, within MMJJ_RS01510 at 296.384 kb on + strand, within MMJJ_RS01510 at 296.409 kb on - strand, within MMJJ_RS01510 at 296.443 kb on + strand, within MMJJ_RS01510 at 296.451 kb on + strand, within MMJJ_RS01510 at 296.463 kb on - strand, within MMJJ_RS01510 at 296.501 kb on - strand, within MMJJ_RS01510 at 296.501 kb on - strand, within MMJJ_RS01510 at 296.586 kb on + strand, within MMJJ_RS01510 at 296.653 kb on - strand, within MMJJ_RS01510 at 296.712 kb on + strand, within MMJJ_RS01510 at 296.744 kb on - strand, within MMJJ_RS01510 at 296.874 kb on - strand, within MMJJ_RS01510 at 296.916 kb on - strand, within MMJJ_RS01510 at 297.015 kb on + strand at 297.066 kb on - strand at 297.069 kb on + strand at 297.073 kb on - strand at 297.074 kb on + strand at 297.076 kb on + strand at 297.088 kb on - strand at 297.107 kb on + strand at 297.115 kb on - strand at 297.120 kb on - strand at 297.121 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.129 kb on + strand at 297.195 kb on + strand at 297.208 kb on - strand at 297.254 kb on - strand at 297.259 kb on - strand at 297.273 kb on - strand, within MMJJ_RS01515 at 297.274 kb on + strand, within MMJJ_RS01515 at 297.321 kb on + strand, within MMJJ_RS01515 at 297.343 kb on + strand, within MMJJ_RS01515 at 297.370 kb on - strand, within MMJJ_RS01515 at 297.371 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.552 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.641 kb on - strand at 297.651 kb on + strand, within MMJJ_RS01520 at 297.717 kb on + strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.779 kb on + strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.807 kb on + strand, within MMJJ_RS01520 at 297.842 kb on - strand at 297.864 kb on + strand at 297.901 kb on - strand at 297.906 kb on - strand at 297.965 kb on - strand, within MMJJ_RS01525 at 297.965 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.977 kb on + strand, within MMJJ_RS01525 at 298.059 kb on - strand, within MMJJ_RS01525
Per-strain Table
Position Strand Gene LocusTag Fraction Formate pooled triplicate planktonic cells remove 294,713 - MMJJ_RS01500 0.70 -3.9 294,748 + MMJJ_RS01500 0.73 -3.0 294,889 + MMJJ_RS01500 0.88 -1.8 294,908 - MMJJ_RS01500 0.90 -2.4 294,994 + -0.2 295,007 - -2.0 295,014 - +1.7 295,028 - +0.0 295,038 + -1.4 295,050 + -3.1 295,067 - -0.4 295,090 + -0.9 295,091 - -0.5 295,093 - +0.1 295,093 - +0.3 295,093 - +1.1 295,093 - +0.0 295,093 - -0.2 295,094 + -0.5 295,096 - -0.4 295,096 - +0.5 295,096 - +0.4 295,096 - -0.1 295,096 - +0.1 295,096 - -0.6 295,097 + -0.3 295,097 + +0.2 295,097 + -0.3 295,097 + -0.3 295,100 + +0.4 295,100 + -1.0 295,108 + +0.7 295,282 - MMJJ_RS01505 0.32 -0.1 295,295 - MMJJ_RS01505 0.35 +0.3 295,295 - MMJJ_RS01505 0.35 +0.2 295,317 - MMJJ_RS01505 0.39 +0.7 295,366 - MMJJ_RS01505 0.49 +0.5 295,367 + MMJJ_RS01505 0.50 -0.5 295,378 - MMJJ_RS01505 0.52 +0.1 295,378 - MMJJ_RS01505 0.52 +0.6 295,379 + MMJJ_RS01505 0.52 -0.2 295,391 + MMJJ_RS01505 0.55 -0.0 295,444 - MMJJ_RS01505 0.66 -0.0 295,563 - +1.9 295,566 - -1.0 295,567 + -2.0 295,609 - -0.3 295,609 - -0.2 295,610 + +0.2 295,610 + -1.1 295,610 + -0.1 295,610 + -0.1 295,637 + +0.1 295,655 - -0.2 295,661 + -0.1 295,667 - -0.2 295,667 - +0.8 295,668 + -0.6 295,668 + +1.0 295,671 - +0.2 295,671 - -0.8 295,672 + +0.0 295,679 - +0.2 295,700 - +0.0 295,701 + +1.3 295,701 + -0.7 295,706 - -0.1 295,706 - +3.1 295,707 + +0.4 295,707 + -0.1 295,788 - -0.4 295,789 + +0.4 295,789 + +0.1 295,793 - +0.2 295,794 + -0.7 295,796 + -0.6 295,797 - +1.1 295,820 - +0.1 295,839 - -0.2 295,839 - -0.4 295,841 - +0.1 295,845 + MMJJ_RS01510 0.10 -0.3 295,863 - MMJJ_RS01510 0.12 -0.8 295,920 - MMJJ_RS01510 0.16 +0.5 295,923 + MMJJ_RS01510 0.16 -0.1 295,954 + MMJJ_RS01510 0.18 +0.8 295,966 - MMJJ_RS01510 0.19 +1.7 295,966 - MMJJ_RS01510 0.19 +0.6 295,967 + MMJJ_RS01510 0.19 -0.1 295,967 + MMJJ_RS01510 0.19 +0.1 295,967 + MMJJ_RS01510 0.19 -0.4 295,986 + MMJJ_RS01510 0.20 +0.6 296,001 + MMJJ_RS01510 0.21 -0.4 296,069 - MMJJ_RS01510 0.26 +0.3 296,096 - MMJJ_RS01510 0.28 -0.0 296,097 + MMJJ_RS01510 0.28 +0.3 296,097 + MMJJ_RS01510 0.28 +0.0 296,122 + MMJJ_RS01510 0.30 +2.7 296,217 - -0.1 296,248 + MMJJ_RS01510 0.39 -0.3 296,265 - -1.1 296,265 - MMJJ_RS01510 0.40 +0.4 296,304 - MMJJ_RS01510 0.43 -0.5 296,337 - MMJJ_RS01510 0.45 +0.5 296,378 + MMJJ_RS01510 0.48 -0.4 296,384 + MMJJ_RS01510 0.49 +1.6 296,409 - MMJJ_RS01510 0.51 +0.1 296,443 + MMJJ_RS01510 0.53 -0.0 296,451 + MMJJ_RS01510 0.54 +0.0 296,463 - MMJJ_RS01510 0.54 +0.3 296,501 - MMJJ_RS01510 0.57 +0.2 296,501 - MMJJ_RS01510 0.57 -0.4 296,586 + MMJJ_RS01510 0.63 -0.1 296,653 - MMJJ_RS01510 0.68 -1.1 296,712 + MMJJ_RS01510 0.72 -0.4 296,744 - MMJJ_RS01510 0.75 -1.1 296,874 - MMJJ_RS01510 0.84 +2.3 296,916 - MMJJ_RS01510 0.87 +0.4 297,015 + -0.4 297,066 - -0.1 297,069 + +0.4 297,073 - +0.3 297,074 + +0.8 297,076 + +0.4 297,088 - +0.9 297,107 + +0.8 297,115 - -0.2 297,120 - +0.7 297,121 + +0.8 297,126 + -0.4 297,126 + +1.2 297,126 + -0.8 297,129 + +3.2 297,195 + +0.0 297,208 - -0.5 297,254 - -0.3 297,259 - +0.1 297,273 - MMJJ_RS01515 0.11 -0.7 297,274 + MMJJ_RS01515 0.11 +2.4 297,321 + MMJJ_RS01515 0.24 +0.1 297,343 + MMJJ_RS01515 0.29 -0.1 297,370 - MMJJ_RS01515 0.36 -0.5 297,371 + MMJJ_RS01515 0.36 +0.3 297,394 + MMJJ_RS01515 0.42 -1.0 297,394 + MMJJ_RS01515 0.42 +0.9 297,504 + MMJJ_RS01515 0.71 -0.3 297,504 + MMJJ_RS01515 0.71 -0.8 297,552 - MMJJ_RS01515 0.83 +1.6 297,567 - MMJJ_RS01515 0.87 -0.9 297,567 - MMJJ_RS01515 0.87 -0.0 297,588 - -0.6 297,588 - +0.5 297,588 - -0.9 297,588 - -0.4 297,588 - -0.5 297,589 + +0.0 297,589 + +0.2 297,589 + +0.4 297,589 + -0.3 297,589 + +0.3 297,589 + +0.2 297,589 + -1.1 297,641 - +0.2 297,651 + MMJJ_RS01520 0.12 -2.7 297,717 + MMJJ_RS01520 0.44 +3.4 297,757 - MMJJ_RS01520 0.64 +0.3 297,757 - MMJJ_RS01520 0.64 -0.3 297,779 + MMJJ_RS01520 0.75 +1.0 297,802 - MMJJ_RS01520 0.86 -1.6 297,802 - MMJJ_RS01520 0.86 +0.1 297,807 + MMJJ_RS01520 0.88 -0.6 297,842 - -1.4 297,864 + -0.2 297,901 - +0.2 297,906 - +0.1 297,965 - MMJJ_RS01525 0.13 +0.2 297,965 - MMJJ_RS01525 0.13 +0.5 297,976 - MMJJ_RS01525 0.14 +0.0 297,976 - MMJJ_RS01525 0.14 +1.3 297,977 + MMJJ_RS01525 0.14 -0.1 298,059 - MMJJ_RS01525 0.23 +0.4
Or see this region's nucleotide sequence