Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22320 and EX31_RS22325 are separated by 140 nucleotides EX31_RS22325 and EX31_RS22330 are separated by 271 nucleotides EX31_RS22330 and EX31_RS22335 are separated by 67 nucleotides
EX31_RS22320: EX31_RS22320 - ammonium transporter AmtB, at 217,121 to 218,410
_RS22320
EX31_RS22325: EX31_RS22325 - acyl-CoA thioesterase II, at 218,551 to 219,417
_RS22325
EX31_RS22330: EX31_RS22330 - hypothetical protein, at 219,689 to 220,270
_RS22330
EX31_RS22335: EX31_RS22335 - MGMT family protein, at 220,338 to 220,646
_RS22335
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.557 kb on + strand, within EX31_RS22320 at 217.557 kb on + strand, within EX31_RS22320 at 217.557 kb on + strand, within EX31_RS22320 at 217.557 kb on + strand, within EX31_RS22320 at 217.557 kb on + strand, within EX31_RS22320 at 217.558 kb on - strand, within EX31_RS22320 at 217.558 kb on - strand, within EX31_RS22320 at 217.558 kb on - strand, within EX31_RS22320 at 217.558 kb on - strand, within EX31_RS22320 at 217.558 kb on - strand, within EX31_RS22320 at 217.607 kb on + strand, within EX31_RS22320 at 217.607 kb on + strand, within EX31_RS22320 at 217.727 kb on + strand, within EX31_RS22320 at 217.727 kb on + strand, within EX31_RS22320 at 217.727 kb on + strand, within EX31_RS22320 at 217.728 kb on - strand, within EX31_RS22320 at 217.728 kb on - strand, within EX31_RS22320 at 217.728 kb on - strand, within EX31_RS22320 at 217.728 kb on - strand, within EX31_RS22320 at 217.814 kb on + strand, within EX31_RS22320 at 217.814 kb on + strand, within EX31_RS22320 at 217.814 kb on + strand, within EX31_RS22320 at 217.814 kb on + strand, within EX31_RS22320 at 217.814 kb on + strand, within EX31_RS22320 at 217.815 kb on - strand, within EX31_RS22320 at 217.815 kb on - strand, within EX31_RS22320 at 218.021 kb on - strand, within EX31_RS22320 at 218.021 kb on - strand, within EX31_RS22320 at 218.059 kb on - strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.160 kb on + strand, within EX31_RS22320 at 218.161 kb on - strand, within EX31_RS22320 at 218.161 kb on - strand, within EX31_RS22320 at 218.181 kb on + strand, within EX31_RS22320 at 218.209 kb on - strand, within EX31_RS22320 at 218.213 kb on + strand, within EX31_RS22320 at 218.214 kb on - strand, within EX31_RS22320 at 218.331 kb on + strand at 218.331 kb on + strand at 218.331 kb on + strand at 218.332 kb on - strand at 218.332 kb on - strand at 218.378 kb on - strand at 218.380 kb on + strand at 218.381 kb on - strand at 218.399 kb on + strand at 218.456 kb on + strand at 218.480 kb on - strand at 218.515 kb on - strand at 218.552 kb on + strand at 218.552 kb on + strand at 218.553 kb on - strand at 218.621 kb on + strand at 218.621 kb on + strand at 218.622 kb on - strand at 218.622 kb on - strand at 218.622 kb on - strand at 218.664 kb on + strand, within EX31_RS22325 at 218.665 kb on - strand, within EX31_RS22325 at 218.678 kb on + strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.679 kb on - strand, within EX31_RS22325 at 218.817 kb on - strand, within EX31_RS22325 at 218.817 kb on - strand, within EX31_RS22325 at 218.825 kb on - strand, within EX31_RS22325 at 218.829 kb on - strand, within EX31_RS22325 at 218.947 kb on - strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 219.026 kb on - strand, within EX31_RS22325 at 219.026 kb on - strand, within EX31_RS22325 at 219.108 kb on + strand, within EX31_RS22325 at 219.109 kb on - strand, within EX31_RS22325 at 219.111 kb on - strand, within EX31_RS22325 at 219.166 kb on - strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.195 kb on + strand, within EX31_RS22325 at 219.196 kb on - strand, within EX31_RS22325 at 219.196 kb on - strand, within EX31_RS22325 at 219.250 kb on - strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.466 kb on + strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.511 kb on + strand at 219.512 kb on - strand at 219.512 kb on - strand at 219.519 kb on + strand at 219.520 kb on - strand at 219.525 kb on + strand at 219.535 kb on + strand at 219.535 kb on + strand at 219.535 kb on + strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.599 kb on - strand at 219.599 kb on - strand at 219.599 kb on - strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.602 kb on - strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.624 kb on - strand at 219.646 kb on - strand at 219.646 kb on - strand at 219.666 kb on + strand at 219.681 kb on + strand at 219.682 kb on - strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.709 kb on - strand at 219.709 kb on - strand at 219.842 kb on - strand, within EX31_RS22330 at 219.999 kb on - strand, within EX31_RS22330 at 220.000 kb on + strand, within EX31_RS22330 at 220.000 kb on + strand, within EX31_RS22330 at 220.001 kb on - strand, within EX31_RS22330 at 220.021 kb on + strand, within EX31_RS22330 at 220.021 kb on + strand, within EX31_RS22330 at 220.031 kb on - strand, within EX31_RS22330 at 220.118 kb on - strand, within EX31_RS22330 at 220.241 kb on - strand at 220.266 kb on - strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.269 kb on - strand at 220.269 kb on - strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.289 kb on + strand at 220.290 kb on - strand at 220.354 kb on + strand at 220.355 kb on - strand at 220.383 kb on - strand, within EX31_RS22335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 217,557 + EX31_RS22320 0.34 +0.5 217,557 + EX31_RS22320 0.34 -1.4 217,557 + EX31_RS22320 0.34 -0.6 217,557 + EX31_RS22320 0.34 -0.7 217,557 + EX31_RS22320 0.34 -0.1 217,558 - EX31_RS22320 0.34 -1.4 217,558 - EX31_RS22320 0.34 -0.8 217,558 - EX31_RS22320 0.34 +1.2 217,558 - EX31_RS22320 0.34 -0.1 217,558 - EX31_RS22320 0.34 -1.1 217,607 + EX31_RS22320 0.38 -0.8 217,607 + EX31_RS22320 0.38 -1.7 217,727 + EX31_RS22320 0.47 -1.0 217,727 + EX31_RS22320 0.47 -0.4 217,727 + EX31_RS22320 0.47 -2.1 217,728 - EX31_RS22320 0.47 -0.2 217,728 - EX31_RS22320 0.47 -0.4 217,728 - EX31_RS22320 0.47 +0.9 217,728 - EX31_RS22320 0.47 -2.4 217,814 + EX31_RS22320 0.54 -0.8 217,814 + EX31_RS22320 0.54 -0.3 217,814 + EX31_RS22320 0.54 -1.1 217,814 + EX31_RS22320 0.54 -1.5 217,814 + EX31_RS22320 0.54 -1.0 217,815 - EX31_RS22320 0.54 -0.9 217,815 - EX31_RS22320 0.54 +0.3 218,021 - EX31_RS22320 0.70 -1.3 218,021 - EX31_RS22320 0.70 +0.2 218,059 - EX31_RS22320 0.73 +0.0 218,160 + EX31_RS22320 0.81 +0.0 218,160 + EX31_RS22320 0.81 +0.2 218,160 + EX31_RS22320 0.81 -0.0 218,160 + EX31_RS22320 0.81 -1.7 218,160 + EX31_RS22320 0.81 -0.5 218,160 + EX31_RS22320 0.81 -1.1 218,160 + EX31_RS22320 0.81 -0.8 218,160 + EX31_RS22320 0.81 -0.4 218,160 + EX31_RS22320 0.81 -1.1 218,160 + EX31_RS22320 0.81 -0.5 218,161 - EX31_RS22320 0.81 -0.4 218,161 - EX31_RS22320 0.81 +1.1 218,181 + EX31_RS22320 0.82 -0.1 218,209 - EX31_RS22320 0.84 -1.4 218,213 + EX31_RS22320 0.85 -0.1 218,214 - EX31_RS22320 0.85 +0.9 218,331 + -1.3 218,331 + -0.8 218,331 + -0.2 218,332 - -1.2 218,332 - -1.0 218,378 - -0.9 218,380 + +0.2 218,381 - +0.2 218,399 + -0.7 218,456 + -0.9 218,480 - +1.0 218,515 - +1.7 218,552 + -0.5 218,552 + +1.4 218,553 - -0.6 218,621 + +1.5 218,621 + -1.0 218,622 - +0.8 218,622 - -0.4 218,622 - -1.4 218,664 + EX31_RS22325 0.13 -0.8 218,665 - EX31_RS22325 0.13 +0.9 218,678 + EX31_RS22325 0.15 +0.0 218,679 - EX31_RS22325 0.15 +0.1 218,679 - EX31_RS22325 0.15 +0.4 218,679 - EX31_RS22325 0.15 +0.7 218,679 - EX31_RS22325 0.15 +0.1 218,679 - EX31_RS22325 0.15 +0.2 218,679 - EX31_RS22325 0.15 +0.4 218,679 - EX31_RS22325 0.15 +0.3 218,817 - EX31_RS22325 0.31 +0.9 218,817 - EX31_RS22325 0.31 -0.3 218,825 - EX31_RS22325 0.32 -0.7 218,829 - EX31_RS22325 0.32 -0.2 218,947 - EX31_RS22325 0.46 -0.7 218,952 + EX31_RS22325 0.46 -1.0 218,952 + EX31_RS22325 0.46 +0.2 218,952 + EX31_RS22325 0.46 -0.2 218,952 + EX31_RS22325 0.46 -0.2 218,953 - EX31_RS22325 0.46 +0.2 218,953 - EX31_RS22325 0.46 +0.6 218,953 - EX31_RS22325 0.46 -0.1 218,953 - EX31_RS22325 0.46 +1.1 219,026 - EX31_RS22325 0.55 +0.3 219,026 - EX31_RS22325 0.55 +0.6 219,108 + EX31_RS22325 0.64 +0.9 219,109 - EX31_RS22325 0.64 +0.0 219,111 - EX31_RS22325 0.65 -0.2 219,166 - EX31_RS22325 0.71 +0.3 219,191 + EX31_RS22325 0.74 -0.3 219,191 + EX31_RS22325 0.74 -0.3 219,191 + EX31_RS22325 0.74 +1.1 219,192 - EX31_RS22325 0.74 -0.4 219,192 - EX31_RS22325 0.74 +0.1 219,192 - EX31_RS22325 0.74 +0.4 219,192 - EX31_RS22325 0.74 -0.3 219,192 - EX31_RS22325 0.74 -0.7 219,192 - EX31_RS22325 0.74 +1.0 219,195 + EX31_RS22325 0.74 +1.6 219,196 - EX31_RS22325 0.74 +0.4 219,196 - EX31_RS22325 0.74 -0.1 219,250 - EX31_RS22325 0.81 +0.2 219,284 + EX31_RS22325 0.85 -0.2 219,284 + EX31_RS22325 0.85 +1.9 219,284 + EX31_RS22325 0.85 +1.6 219,284 + EX31_RS22325 0.85 -1.4 219,284 + EX31_RS22325 0.85 +1.9 219,284 + EX31_RS22325 0.85 +1.9 219,285 - EX31_RS22325 0.85 -1.9 219,285 - EX31_RS22325 0.85 -0.9 219,285 - EX31_RS22325 0.85 +0.7 219,285 - EX31_RS22325 0.85 +0.2 219,285 - EX31_RS22325 0.85 +1.0 219,285 - EX31_RS22325 0.85 +1.4 219,286 + EX31_RS22325 0.85 -0.3 219,286 + EX31_RS22325 0.85 +0.9 219,286 + EX31_RS22325 0.85 -0.7 219,286 + EX31_RS22325 0.85 -0.7 219,287 - EX31_RS22325 0.85 +0.1 219,287 - EX31_RS22325 0.85 +0.7 219,287 - EX31_RS22325 0.85 -1.4 219,287 - EX31_RS22325 0.85 +0.0 219,287 - EX31_RS22325 0.85 +1.3 219,287 - EX31_RS22325 0.85 +0.1 219,287 - EX31_RS22325 0.85 -0.7 219,287 - EX31_RS22325 0.85 -0.7 219,287 - EX31_RS22325 0.85 +0.5 219,287 - EX31_RS22325 0.85 +0.7 219,287 - EX31_RS22325 0.85 +0.3 219,287 - EX31_RS22325 0.85 -0.4 219,287 - EX31_RS22325 0.85 -0.6 219,326 - EX31_RS22325 0.89 +1.2 219,326 - EX31_RS22325 0.89 -0.8 219,326 - EX31_RS22325 0.89 -1.1 219,452 + +1.3 219,452 + +0.5 219,452 + -1.0 219,452 + +1.0 219,452 + +0.6 219,452 + -1.0 219,452 + -0.2 219,452 + +0.8 219,452 + -0.2 219,453 - -0.1 219,453 - +1.1 219,453 - +0.2 219,453 - +0.5 219,453 - -0.9 219,453 - -0.0 219,453 - +0.1 219,466 + +0.8 219,492 - -0.9 219,492 - -1.3 219,492 - -0.7 219,492 - +1.4 219,511 + -0.2 219,512 - -0.0 219,512 - +1.2 219,519 + +0.5 219,520 - -0.2 219,525 + +0.2 219,535 + +0.1 219,535 + +0.2 219,535 + +1.3 219,536 - -0.1 219,536 - -0.4 219,536 - +0.6 219,536 - -0.7 219,536 - +0.5 219,598 + +1.3 219,598 + +0.4 219,599 - +0.1 219,599 - -0.4 219,599 - -0.7 219,601 + -0.2 219,601 + -1.4 219,601 + +0.0 219,601 + -0.1 219,602 - -0.9 219,605 + +0.8 219,605 + -0.4 219,605 + +1.6 219,605 + -1.6 219,605 + +0.8 219,605 + +0.8 219,605 + -0.3 219,605 + -0.7 219,605 + +0.3 219,606 - -1.7 219,606 - -0.9 219,606 - -0.8 219,606 - -1.3 219,606 - -0.2 219,606 - -1.0 219,606 - +1.9 219,624 - +0.7 219,646 - +0.7 219,646 - +0.4 219,666 + +1.0 219,681 + +0.6 219,682 - +1.2 219,705 + +1.8 219,705 + +0.9 219,705 + -1.0 219,705 + +2.4 219,708 + +1.7 219,708 + +1.9 219,708 + +0.8 219,708 + +0.3 219,709 - -0.7 219,709 - -1.2 219,842 - EX31_RS22330 0.26 -0.9 219,999 - EX31_RS22330 0.53 +0.8 220,000 + EX31_RS22330 0.53 +0.5 220,000 + EX31_RS22330 0.53 -2.0 220,001 - EX31_RS22330 0.54 -1.0 220,021 + EX31_RS22330 0.57 +0.4 220,021 + EX31_RS22330 0.57 -0.1 220,031 - EX31_RS22330 0.59 -1.3 220,118 - EX31_RS22330 0.74 +1.4 220,241 - -2.7 220,266 - +1.1 220,268 + +0.1 220,268 + +0.3 220,268 + +0.5 220,269 - +0.7 220,269 - +0.3 220,280 + -1.2 220,280 + -0.1 220,280 + -1.4 220,280 + +0.8 220,280 + +0.4 220,280 + +1.4 220,280 + -0.9 220,280 + +1.6 220,280 + +0.2 220,280 + +0.4 220,280 + +0.2 220,280 + -1.6 220,280 + +0.6 220,280 + +1.4 220,281 - -0.9 220,281 - +0.5 220,281 - -0.1 220,281 - -0.8 220,281 - +0.2 220,281 - -0.3 220,281 - +0.4 220,281 - +1.9 220,281 - -1.0 220,281 - -0.2 220,281 - +0.7 220,281 - -0.4 220,289 + -0.6 220,290 - -0.6 220,354 + -0.3 220,355 - +0.5 220,383 - EX31_RS22335 0.15 +0.1
Or see this region's nucleotide sequence