Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22110 and EX31_RS22115 are separated by 81 nucleotides EX31_RS22115 and EX31_RS22120 are separated by 160 nucleotides EX31_RS22120 and EX31_RS22125 are separated by 9 nucleotides
EX31_RS22110: EX31_RS22110 - protein translocase subunit SecF, at 171,340 to 172,314
_RS22110
EX31_RS22115: EX31_RS22115 - DUF3251 domain-containing protein, at 172,396 to 172,959
_RS22115
EX31_RS22120: EX31_RS22120 - transcriptional regulator NrdR, at 173,120 to 173,569
_RS22120
EX31_RS22125: EX31_RS22125 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 173,579 to 174,715
_RS22125
Position (kb) 173
174 Strain fitness (log2 ratio) -2
-1
0
1
2 at 172.312 kb on + strand at 172.312 kb on + strand at 172.312 kb on + strand at 172.312 kb on + strand at 172.313 kb on - strand at 172.313 kb on - strand at 172.397 kb on + strand at 172.398 kb on - strand at 172.437 kb on + strand at 172.437 kb on + strand at 172.437 kb on + strand at 172.437 kb on + strand at 172.437 kb on + strand at 172.438 kb on - strand at 172.438 kb on - strand at 172.438 kb on - strand at 172.438 kb on - strand at 172.438 kb on - strand at 172.438 kb on - strand at 172.441 kb on - strand at 172.441 kb on - strand at 172.441 kb on - strand at 172.536 kb on + strand, within EX31_RS22115 at 172.615 kb on - strand, within EX31_RS22115 at 172.709 kb on - strand, within EX31_RS22115 at 172.709 kb on - strand, within EX31_RS22115 at 172.709 kb on - strand, within EX31_RS22115 at 172.709 kb on - strand, within EX31_RS22115 at 172.709 kb on - strand, within EX31_RS22115 at 172.719 kb on + strand, within EX31_RS22115 at 172.720 kb on - strand, within EX31_RS22115 at 172.720 kb on - strand, within EX31_RS22115 at 172.720 kb on - strand, within EX31_RS22115 at 172.720 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.723 kb on - strand, within EX31_RS22115 at 172.763 kb on + strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.764 kb on - strand, within EX31_RS22115 at 172.773 kb on + strand, within EX31_RS22115 at 172.773 kb on + strand, within EX31_RS22115 at 172.773 kb on + strand, within EX31_RS22115 at 172.774 kb on - strand, within EX31_RS22115 at 173.017 kb on + strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.018 kb on - strand at 173.021 kb on + strand at 173.021 kb on + strand at 173.021 kb on + strand at 173.021 kb on + strand at 173.021 kb on + strand at 173.021 kb on + strand at 173.022 kb on - strand at 173.022 kb on - strand at 173.022 kb on - strand at 173.022 kb on - strand at 173.022 kb on - strand at 173.047 kb on + strand at 173.047 kb on + strand at 173.047 kb on + strand at 173.047 kb on + strand at 173.048 kb on - strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.086 kb on + strand at 173.119 kb on + strand at 173.119 kb on + strand at 173.119 kb on + strand at 173.119 kb on + strand at 173.119 kb on + strand at 173.119 kb on + strand at 173.149 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.152 kb on + strand at 173.175 kb on + strand, within EX31_RS22120 at 173.275 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.392 kb on + strand, within EX31_RS22120 at 173.489 kb on + strand, within EX31_RS22120 at 173.489 kb on + strand, within EX31_RS22120 at 173.489 kb on + strand, within EX31_RS22120 at 173.489 kb on + strand, within EX31_RS22120 at 173.489 kb on + strand, within EX31_RS22120 at 173.510 kb on + strand, within EX31_RS22120 at 173.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 172,312 + -0.2 172,312 + +0.0 172,312 + +0.3 172,312 + -0.4 172,313 - +0.3 172,313 - +0.7 172,397 + +2.7 172,398 - +0.9 172,437 + -0.7 172,437 + -0.1 172,437 + +0.0 172,437 + -0.1 172,437 + -0.1 172,438 - -0.0 172,438 - -0.7 172,438 - +0.7 172,438 - -0.2 172,438 - -0.0 172,438 - +0.3 172,441 - +0.4 172,441 - -0.6 172,441 - +1.4 172,536 + EX31_RS22115 0.25 +1.2 172,615 - EX31_RS22115 0.39 +1.2 172,709 - EX31_RS22115 0.55 -0.5 172,709 - EX31_RS22115 0.55 +0.5 172,709 - EX31_RS22115 0.55 -0.6 172,709 - EX31_RS22115 0.55 -0.2 172,709 - EX31_RS22115 0.55 +1.5 172,719 + EX31_RS22115 0.57 +0.6 172,720 - EX31_RS22115 0.57 -1.3 172,720 - EX31_RS22115 0.57 +0.3 172,720 - EX31_RS22115 0.57 +0.7 172,720 - EX31_RS22115 0.57 -0.4 172,723 - EX31_RS22115 0.58 -0.1 172,723 - EX31_RS22115 0.58 +1.0 172,723 - EX31_RS22115 0.58 +1.2 172,723 - EX31_RS22115 0.58 -0.3 172,723 - EX31_RS22115 0.58 +0.2 172,723 - EX31_RS22115 0.58 +1.2 172,723 - EX31_RS22115 0.58 +0.2 172,763 + EX31_RS22115 0.65 -1.1 172,764 - EX31_RS22115 0.65 +0.9 172,764 - EX31_RS22115 0.65 -0.5 172,764 - EX31_RS22115 0.65 +1.2 172,764 - EX31_RS22115 0.65 -1.1 172,764 - EX31_RS22115 0.65 +0.3 172,764 - EX31_RS22115 0.65 +1.3 172,773 + EX31_RS22115 0.67 +0.5 172,773 + EX31_RS22115 0.67 +0.7 172,773 + EX31_RS22115 0.67 +0.4 172,774 - EX31_RS22115 0.67 -0.3 173,017 + +1.8 173,018 - -0.8 173,018 - +1.4 173,018 - -0.7 173,018 - -1.0 173,018 - -1.0 173,018 - +0.4 173,018 - -0.4 173,018 - +0.0 173,018 - -0.9 173,021 + -0.1 173,021 + +2.1 173,021 + +0.4 173,021 + -0.3 173,021 + +0.2 173,021 + -0.3 173,022 - +0.8 173,022 - +0.2 173,022 - +1.3 173,022 - +0.7 173,022 - -0.4 173,047 + -2.3 173,047 + -0.2 173,047 + +0.2 173,047 + +0.9 173,048 - +0.9 173,086 + +0.9 173,086 + +0.9 173,086 + +0.2 173,086 + -0.6 173,086 + -0.7 173,086 + -1.3 173,086 + +0.4 173,086 + +0.2 173,086 + -0.2 173,119 + -0.4 173,119 + -2.3 173,119 + -0.1 173,119 + -0.7 173,119 + -0.4 173,119 + -0.2 173,149 + -0.5 173,152 + -1.3 173,152 + -1.3 173,152 + +1.2 173,152 + -0.1 173,152 + -0.2 173,152 + +0.2 173,152 + -0.5 173,175 + EX31_RS22120 0.12 -0.8 173,275 + EX31_RS22120 0.34 -1.7 173,392 + EX31_RS22120 0.60 +0.5 173,392 + EX31_RS22120 0.60 -2.2 173,392 + EX31_RS22120 0.60 -0.9 173,392 + EX31_RS22120 0.60 +0.2 173,392 + EX31_RS22120 0.60 -1.1 173,392 + EX31_RS22120 0.60 -1.1 173,392 + EX31_RS22120 0.60 -2.3 173,392 + EX31_RS22120 0.60 -0.7 173,392 + EX31_RS22120 0.60 -0.7 173,392 + EX31_RS22120 0.60 -0.5 173,489 + EX31_RS22120 0.82 -1.1 173,489 + EX31_RS22120 0.82 +0.1 173,489 + EX31_RS22120 0.82 +0.3 173,489 + EX31_RS22120 0.82 +0.8 173,489 + EX31_RS22120 0.82 +0.1 173,510 + EX31_RS22120 0.87 -0.3 173,527 + -0.4
Or see this region's nucleotide sequence