Experiment: L-Serine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12520 and EX31_RS12525 are separated by 896 nucleotides EX31_RS12525 and EX31_RS12530 are separated by 65 nucleotides EX31_RS12530 and EX31_RS12535 are separated by 9 nucleotides
EX31_RS12520: EX31_RS12520 - aerobic respiration two-component sensor histidine kinase ArcB, at 173,644 to 175,983
_RS12520
EX31_RS12525: EX31_RS12525 - monofunctional biosynthetic peptidoglycan transglycosylase, at 176,880 to 177,602
_RS12525
EX31_RS12530: EX31_RS12530 - divisome-associated lipoprotein YraP, at 177,668 to 178,243
_RS12530
EX31_RS12535: EX31_RS12535 - DnaA initiator-associating protein DiaA, at 178,253 to 178,843
_RS12535
Position (kb) 176
177
178 Strain fitness (log2 ratio) -2
-1
0
1
2 at 175.946 kb on + strand at 175.946 kb on + strand at 175.946 kb on + strand at 175.947 kb on - strand at 175.947 kb on - strand at 175.950 kb on - strand at 176.010 kb on + strand at 176.037 kb on - strand at 176.046 kb on + strand at 176.096 kb on + strand at 176.096 kb on + strand at 176.097 kb on - strand at 176.097 kb on - strand at 176.097 kb on - strand at 176.099 kb on + strand at 176.099 kb on + strand at 176.099 kb on + strand at 176.099 kb on + strand at 176.099 kb on + strand at 176.099 kb on + strand at 176.100 kb on - strand at 176.100 kb on - strand at 176.100 kb on - strand at 176.100 kb on - strand at 176.100 kb on - strand at 176.109 kb on - strand at 176.120 kb on + strand at 176.121 kb on - strand at 176.127 kb on + strand at 176.127 kb on + strand at 176.127 kb on + strand at 176.127 kb on + strand at 176.128 kb on - strand at 176.128 kb on - strand at 176.128 kb on - strand at 176.128 kb on - strand at 176.129 kb on + strand at 176.130 kb on - strand at 176.130 kb on - strand at 176.130 kb on - strand at 176.168 kb on + strand at 176.168 kb on + strand at 176.168 kb on + strand at 176.169 kb on - strand at 176.169 kb on - strand at 176.169 kb on - strand at 176.169 kb on - strand at 176.169 kb on - strand at 176.173 kb on + strand at 176.227 kb on - strand at 176.250 kb on + strand at 176.289 kb on - strand at 176.289 kb on - strand at 176.374 kb on - strand at 176.376 kb on + strand at 176.463 kb on + strand at 176.463 kb on + strand at 176.464 kb on - strand at 176.469 kb on + strand at 176.519 kb on + strand at 176.519 kb on + strand at 176.520 kb on - strand at 176.533 kb on + strand at 176.533 kb on + strand at 176.533 kb on + strand at 176.533 kb on + strand at 176.533 kb on + strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.534 kb on - strand at 176.542 kb on + strand at 176.542 kb on + strand at 176.543 kb on - strand at 176.543 kb on - strand at 176.662 kb on + strand at 176.724 kb on + strand at 176.733 kb on - strand at 176.777 kb on - strand at 176.836 kb on - strand at 176.848 kb on + strand at 176.849 kb on - strand at 176.849 kb on - strand at 176.877 kb on + strand at 176.877 kb on + strand at 176.923 kb on + strand at 176.923 kb on + strand at 176.924 kb on - strand at 176.924 kb on - strand at 176.944 kb on + strand at 176.944 kb on + strand at 176.950 kb on + strand at 176.950 kb on + strand at 176.950 kb on + strand at 176.950 kb on + strand at 176.950 kb on + strand at 176.950 kb on + strand at 176.960 kb on - strand, within EX31_RS12525 at 177.060 kb on + strand, within EX31_RS12525 at 177.060 kb on + strand, within EX31_RS12525 at 177.176 kb on + strand, within EX31_RS12525 at 177.176 kb on + strand, within EX31_RS12525 at 177.176 kb on + strand, within EX31_RS12525 at 177.177 kb on - strand, within EX31_RS12525 at 177.177 kb on - strand, within EX31_RS12525 at 177.177 kb on - strand, within EX31_RS12525 at 177.229 kb on + strand, within EX31_RS12525 at 177.229 kb on + strand, within EX31_RS12525 at 177.284 kb on + strand, within EX31_RS12525 at 177.284 kb on + strand, within EX31_RS12525 at 177.285 kb on - strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.355 kb on - strand, within EX31_RS12525 at 177.355 kb on - strand, within EX31_RS12525 at 177.422 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.523 kb on + strand, within EX31_RS12525 at 177.523 kb on + strand, within EX31_RS12525 at 177.524 kb on - strand, within EX31_RS12525 at 177.524 kb on - strand, within EX31_RS12525 at 177.590 kb on + strand at 177.590 kb on + strand at 177.591 kb on - strand at 177.600 kb on + strand at 177.600 kb on + strand at 177.654 kb on + strand at 177.678 kb on + strand at 177.678 kb on + strand at 177.808 kb on + strand, within EX31_RS12530 at 177.809 kb on - strand, within EX31_RS12530 at 177.836 kb on + strand, within EX31_RS12530 at 177.868 kb on + strand, within EX31_RS12530 at 177.868 kb on + strand, within EX31_RS12530 at 177.869 kb on - strand, within EX31_RS12530 at 177.902 kb on - strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 178.012 kb on + strand, within EX31_RS12530 at 178.020 kb on + strand, within EX31_RS12530 at 178.066 kb on + strand, within EX31_RS12530 at 178.078 kb on + strand, within EX31_RS12530 at 178.078 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.196 kb on + strand at 178.196 kb on + strand at 178.196 kb on + strand at 178.196 kb on + strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.212 kb on - strand at 178.212 kb on - strand at 178.217 kb on + strand at 178.217 kb on + strand at 178.218 kb on - strand at 178.218 kb on - strand at 178.255 kb on - strand at 178.282 kb on + strand at 178.282 kb on + strand at 178.283 kb on - strand at 178.286 kb on + strand at 178.287 kb on - strand at 178.306 kb on + strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.318 kb on + strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.435 kb on + strand, within EX31_RS12535 at 178.472 kb on - strand, within EX31_RS12535 at 178.526 kb on + strand, within EX31_RS12535 at 178.544 kb on - strand, within EX31_RS12535 at 178.549 kb on - strand, within EX31_RS12535 at 178.549 kb on - strand, within EX31_RS12535 at 178.597 kb on + strand, within EX31_RS12535 at 178.597 kb on + strand, within EX31_RS12535 at 178.598 kb on - strand, within EX31_RS12535 at 178.598 kb on - strand, within EX31_RS12535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; shaking remove 175,946 + -0.7 175,946 + -0.0 175,946 + -0.7 175,947 - +0.1 175,947 - +0.6 175,950 - +0.5 176,010 + +0.7 176,037 - +0.6 176,046 + +0.6 176,096 + -0.1 176,096 + +0.7 176,097 - +0.4 176,097 - -1.3 176,097 - -0.4 176,099 + -0.8 176,099 + +0.3 176,099 + +0.4 176,099 + +0.7 176,099 + +0.2 176,099 + +0.7 176,100 - -1.3 176,100 - -1.6 176,100 - -0.4 176,100 - -1.2 176,100 - +0.9 176,109 - +0.6 176,120 + -0.8 176,121 - +0.4 176,127 + +0.2 176,127 + -0.7 176,127 + +1.6 176,127 + +0.8 176,128 - +0.9 176,128 - +0.3 176,128 - +0.3 176,128 - -0.7 176,129 + +0.6 176,130 - -0.1 176,130 - -0.1 176,130 - -0.7 176,168 + +0.3 176,168 + +1.1 176,168 + +0.5 176,169 - +1.1 176,169 - +0.3 176,169 - +0.2 176,169 - +1.2 176,169 - -0.2 176,173 + -0.0 176,227 - +0.3 176,250 + +0.4 176,289 - +0.6 176,289 - +0.5 176,374 - -0.2 176,376 + -0.2 176,463 + +0.0 176,463 + +0.2 176,464 - +0.4 176,469 + +0.4 176,519 + +0.8 176,519 + -0.6 176,520 - -0.7 176,533 + -0.3 176,533 + +0.5 176,533 + +1.2 176,533 + -0.4 176,533 + +0.4 176,534 - -1.8 176,534 - -0.3 176,534 - +0.6 176,534 - -0.5 176,534 - +2.5 176,534 - +0.4 176,534 - +0.7 176,534 - +0.4 176,534 - -0.4 176,542 + -0.4 176,542 + +0.1 176,543 - +1.1 176,543 - -1.2 176,662 + -0.4 176,724 + -0.2 176,733 - +0.3 176,777 - -0.3 176,836 - +1.0 176,848 + -0.3 176,849 - -0.8 176,849 - -0.9 176,877 + +0.7 176,877 + -0.1 176,923 + +0.5 176,923 + +0.3 176,924 - -0.9 176,924 - +0.3 176,944 + +0.4 176,944 + +0.7 176,950 + +0.2 176,950 + +0.6 176,950 + -0.8 176,950 + -0.0 176,950 + -1.7 176,950 + -0.1 176,960 - EX31_RS12525 0.11 +0.7 177,060 + EX31_RS12525 0.25 -0.2 177,060 + EX31_RS12525 0.25 -0.4 177,176 + EX31_RS12525 0.41 +0.5 177,176 + EX31_RS12525 0.41 +0.6 177,176 + EX31_RS12525 0.41 -0.4 177,177 - EX31_RS12525 0.41 +1.5 177,177 - EX31_RS12525 0.41 +1.0 177,177 - EX31_RS12525 0.41 +0.8 177,229 + EX31_RS12525 0.48 -0.0 177,229 + EX31_RS12525 0.48 -0.9 177,284 + EX31_RS12525 0.56 +0.6 177,284 + EX31_RS12525 0.56 +1.3 177,285 - EX31_RS12525 0.56 +0.8 177,354 + EX31_RS12525 0.66 -0.4 177,354 + EX31_RS12525 0.66 +1.1 177,354 + EX31_RS12525 0.66 +0.2 177,355 - EX31_RS12525 0.66 +1.5 177,355 - EX31_RS12525 0.66 -1.6 177,422 + EX31_RS12525 0.75 -0.4 177,516 + EX31_RS12525 0.88 -0.1 177,516 + EX31_RS12525 0.88 -0.4 177,516 + EX31_RS12525 0.88 +0.4 177,516 + EX31_RS12525 0.88 +0.6 177,516 + EX31_RS12525 0.88 +1.8 177,517 - EX31_RS12525 0.88 +0.7 177,517 - EX31_RS12525 0.88 +0.2 177,517 - EX31_RS12525 0.88 +1.6 177,517 - EX31_RS12525 0.88 +0.1 177,517 - EX31_RS12525 0.88 +0.6 177,517 - EX31_RS12525 0.88 -0.6 177,517 - EX31_RS12525 0.88 -1.3 177,517 - EX31_RS12525 0.88 -0.2 177,517 - EX31_RS12525 0.88 -1.0 177,517 - EX31_RS12525 0.88 +0.4 177,523 + EX31_RS12525 0.89 +1.2 177,523 + EX31_RS12525 0.89 +2.2 177,524 - EX31_RS12525 0.89 +0.6 177,524 - EX31_RS12525 0.89 +1.0 177,590 + +0.5 177,590 + +0.1 177,591 - -0.9 177,600 + -0.9 177,600 + +0.8 177,654 + +0.7 177,678 + +0.4 177,678 + -0.3 177,808 + EX31_RS12530 0.24 +1.4 177,809 - EX31_RS12530 0.24 -0.6 177,836 + EX31_RS12530 0.29 +0.8 177,868 + EX31_RS12530 0.35 +1.5 177,868 + EX31_RS12530 0.35 +0.5 177,869 - EX31_RS12530 0.35 -0.1 177,902 - EX31_RS12530 0.41 -0.2 177,921 + EX31_RS12530 0.44 -0.6 177,921 + EX31_RS12530 0.44 +0.5 177,921 + EX31_RS12530 0.44 -0.2 177,921 + EX31_RS12530 0.44 +0.6 177,921 + EX31_RS12530 0.44 +1.3 177,922 - EX31_RS12530 0.44 +0.3 177,922 - EX31_RS12530 0.44 +0.0 177,922 - EX31_RS12530 0.44 -0.3 177,922 - EX31_RS12530 0.44 +0.5 177,922 - EX31_RS12530 0.44 +0.1 177,922 - EX31_RS12530 0.44 +1.2 177,922 - EX31_RS12530 0.44 +0.3 177,922 - EX31_RS12530 0.44 -0.5 178,012 + EX31_RS12530 0.60 +1.8 178,020 + EX31_RS12530 0.61 +0.7 178,066 + EX31_RS12530 0.69 +0.1 178,078 + EX31_RS12530 0.71 +0.6 178,078 + EX31_RS12530 0.71 +0.7 178,126 + EX31_RS12530 0.80 -0.7 178,126 + EX31_RS12530 0.80 +0.2 178,126 + EX31_RS12530 0.80 +0.5 178,126 + EX31_RS12530 0.80 +1.0 178,127 - EX31_RS12530 0.80 -0.2 178,127 - EX31_RS12530 0.80 +0.8 178,127 - EX31_RS12530 0.80 +1.4 178,127 - EX31_RS12530 0.80 +0.5 178,127 - EX31_RS12530 0.80 +0.1 178,196 + -0.1 178,196 + +1.0 178,196 + +0.4 178,196 + +0.9 178,197 - -0.1 178,197 - +0.8 178,197 - +1.0 178,197 - +0.5 178,212 - -1.0 178,212 - -0.1 178,217 + +0.6 178,217 + +0.3 178,218 - +1.5 178,218 - +0.2 178,255 - +0.6 178,282 + +2.5 178,282 + +0.3 178,283 - +0.2 178,286 + +1.3 178,287 - +0.3 178,306 + +2.3 178,307 - +0.7 178,307 - -0.7 178,307 - +0.4 178,307 - +0.1 178,307 - +0.7 178,307 - +0.6 178,318 + EX31_RS12535 0.11 +1.0 178,319 - EX31_RS12535 0.11 +0.5 178,319 - EX31_RS12535 0.11 +0.9 178,319 - EX31_RS12535 0.11 +0.6 178,319 - EX31_RS12535 0.11 +1.0 178,435 + EX31_RS12535 0.31 +0.8 178,472 - EX31_RS12535 0.37 +0.7 178,526 + EX31_RS12535 0.46 -0.5 178,544 - EX31_RS12535 0.49 +0.6 178,549 - EX31_RS12535 0.50 -0.3 178,549 - EX31_RS12535 0.50 +0.3 178,597 + EX31_RS12535 0.58 -1.3 178,597 + EX31_RS12535 0.58 +2.3 178,598 - EX31_RS12535 0.58 -1.0 178,598 - EX31_RS12535 0.58 -1.0
Or see this region's nucleotide sequence