Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22650 and CA265_RS22655 are separated by 134 nucleotides CA265_RS22655 and CA265_RS22660 are separated by 323 nucleotides
CA265_RS22650: CA265_RS22650 - branched chain amino acid aminotransferase, at 5,546,320 to 5,547,384
_RS22650
CA265_RS22655: CA265_RS22655 - hypothetical protein, at 5,547,519 to 5,547,983
_RS22655
CA265_RS22660: CA265_RS22660 - DNA repair protein RadA, at 5,548,307 to 5,549,683
_RS22660
Position (kb)
5547
5548 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5546.544 kb on - strand, within CA265_RS22650 at 5546.552 kb on - strand, within CA265_RS22650 at 5546.575 kb on - strand, within CA265_RS22650 at 5546.662 kb on - strand, within CA265_RS22650 at 5546.723 kb on - strand, within CA265_RS22650 at 5546.729 kb on + strand, within CA265_RS22650 at 5546.731 kb on + strand, within CA265_RS22650 at 5546.732 kb on - strand, within CA265_RS22650 at 5546.732 kb on - strand, within CA265_RS22650 at 5546.805 kb on - strand, within CA265_RS22650 at 5546.831 kb on - strand, within CA265_RS22650 at 5546.831 kb on - strand, within CA265_RS22650 at 5546.914 kb on - strand, within CA265_RS22650 at 5546.930 kb on + strand, within CA265_RS22650 at 5546.931 kb on - strand, within CA265_RS22650 at 5547.004 kb on - strand, within CA265_RS22650 at 5547.103 kb on - strand, within CA265_RS22650 at 5547.135 kb on - strand, within CA265_RS22650 at 5547.151 kb on - strand, within CA265_RS22650 at 5547.301 kb on - strand at 5547.301 kb on - strand at 5547.301 kb on - strand at 5547.380 kb on + strand at 5547.380 kb on + strand at 5547.381 kb on - strand at 5547.382 kb on + strand at 5547.420 kb on + strand at 5547.421 kb on - strand at 5547.477 kb on + strand at 5547.520 kb on + strand at 5547.693 kb on + strand, within CA265_RS22655 at 5547.693 kb on + strand, within CA265_RS22655 at 5547.693 kb on + strand, within CA265_RS22655 at 5547.694 kb on - strand, within CA265_RS22655 at 5547.762 kb on + strand, within CA265_RS22655 at 5547.913 kb on + strand, within CA265_RS22655 at 5547.914 kb on + strand, within CA265_RS22655 at 5547.914 kb on + strand, within CA265_RS22655 at 5547.915 kb on - strand, within CA265_RS22655 at 5547.990 kb on - strand at 5548.092 kb on - strand at 5548.093 kb on + strand at 5548.305 kb on - strand at 5548.305 kb on - strand at 5548.308 kb on + strand at 5548.398 kb on + strand at 5548.398 kb on + strand at 5548.399 kb on - strand at 5548.408 kb on + strand at 5548.467 kb on - strand, within CA265_RS22660 at 5548.493 kb on + strand, within CA265_RS22660 at 5548.494 kb on - strand, within CA265_RS22660 at 5548.610 kb on - strand, within CA265_RS22660 at 5548.631 kb on - strand, within CA265_RS22660 at 5548.675 kb on + strand, within CA265_RS22660 at 5548.675 kb on + strand, within CA265_RS22660 at 5548.676 kb on - strand, within CA265_RS22660 at 5548.676 kb on - strand, within CA265_RS22660 at 5548.725 kb on + strand, within CA265_RS22660 at 5548.781 kb on + strand, within CA265_RS22660 at 5548.885 kb on - strand, within CA265_RS22660 at 5548.921 kb on + strand, within CA265_RS22660 at 5548.921 kb on + strand, within CA265_RS22660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,546,544 - CA265_RS22650 0.21 +0.7 5,546,552 - CA265_RS22650 0.22 -0.7 5,546,575 - CA265_RS22650 0.24 -0.7 5,546,662 - CA265_RS22650 0.32 -1.3 5,546,723 - CA265_RS22650 0.38 +1.1 5,546,729 + CA265_RS22650 0.38 -1.7 5,546,731 + CA265_RS22650 0.39 +0.7 5,546,732 - CA265_RS22650 0.39 +0.5 5,546,732 - CA265_RS22650 0.39 -1.4 5,546,805 - CA265_RS22650 0.46 +0.9 5,546,831 - CA265_RS22650 0.48 +1.3 5,546,831 - CA265_RS22650 0.48 +0.3 5,546,914 - CA265_RS22650 0.56 +0.2 5,546,930 + CA265_RS22650 0.57 +0.9 5,546,931 - CA265_RS22650 0.57 -0.0 5,547,004 - CA265_RS22650 0.64 +0.7 5,547,103 - CA265_RS22650 0.74 +1.1 5,547,135 - CA265_RS22650 0.77 -0.7 5,547,151 - CA265_RS22650 0.78 +0.6 5,547,301 - +0.2 5,547,301 - +0.4 5,547,301 - +0.1 5,547,380 + +1.4 5,547,380 + -0.6 5,547,381 - +0.7 5,547,382 + -0.1 5,547,420 + +0.3 5,547,421 - +0.9 5,547,477 + -1.4 5,547,520 + +0.5 5,547,693 + CA265_RS22655 0.37 +0.5 5,547,693 + CA265_RS22655 0.37 +1.1 5,547,693 + CA265_RS22655 0.37 +0.6 5,547,694 - CA265_RS22655 0.38 +0.5 5,547,762 + CA265_RS22655 0.52 +0.7 5,547,913 + CA265_RS22655 0.85 -0.4 5,547,914 + CA265_RS22655 0.85 +0.0 5,547,914 + CA265_RS22655 0.85 -0.5 5,547,915 - CA265_RS22655 0.85 +1.3 5,547,990 - -0.3 5,548,092 - +0.9 5,548,093 + -0.6 5,548,305 - +3.2 5,548,305 - -0.6 5,548,308 + -0.9 5,548,398 + +0.3 5,548,398 + +0.2 5,548,399 - -1.4 5,548,408 + +0.3 5,548,467 - CA265_RS22660 0.12 -0.1 5,548,493 + CA265_RS22660 0.14 -0.4 5,548,494 - CA265_RS22660 0.14 -0.4 5,548,610 - CA265_RS22660 0.22 -1.5 5,548,631 - CA265_RS22660 0.24 +0.4 5,548,675 + CA265_RS22660 0.27 -0.4 5,548,675 + CA265_RS22660 0.27 +1.3 5,548,676 - CA265_RS22660 0.27 +1.1 5,548,676 - CA265_RS22660 0.27 +0.0 5,548,725 + CA265_RS22660 0.30 +0.0 5,548,781 + CA265_RS22660 0.34 -2.5 5,548,885 - CA265_RS22660 0.42 +0.7 5,548,921 + CA265_RS22660 0.45 -0.2 5,548,921 + CA265_RS22660 0.45 +0.7
Or see this region's nucleotide sequence