Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20685 and CA265_RS20690 overlap by 4 nucleotides CA265_RS20690 and CA265_RS20695 overlap by 4 nucleotides CA265_RS20695 and CA265_RS20700 are separated by 11 nucleotides CA265_RS20700 and CA265_RS20705 are separated by 33 nucleotides CA265_RS20705 and CA265_RS20710 overlap by 1 nucleotides CA265_RS20710 and CA265_RS20715 overlap by 29 nucleotides
CA265_RS20685: CA265_RS20685 - hypothetical protein, at 5,117,403 to 5,118,038
_RS20685
CA265_RS20690: CA265_RS20690 - hypothetical protein, at 5,118,035 to 5,118,415
_RS20690
CA265_RS20695: CA265_RS20695 - hypothetical protein, at 5,118,412 to 5,118,744
_RS20695
CA265_RS20700: CA265_RS20700 - plasmid transfer protein, at 5,118,756 to 5,119,058
_RS20700
CA265_RS20705: CA265_RS20705 - conjugal transfer protein, at 5,119,092 to 5,119,403
_RS20705
CA265_RS20710: CA265_RS20710 - DUF4133 domain-containing protein, at 5,119,403 to 5,119,708
_RS20710
CA265_RS20715: CA265_RS20715 - conjugal transfer protein TraG, at 5,119,680 to 5,122,130
_RS20715
Position (kb) 5118
5119 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5117.580 kb on + strand, within CA265_RS20685 at 5117.580 kb on + strand, within CA265_RS20685 at 5117.642 kb on + strand, within CA265_RS20685 at 5117.642 kb on + strand, within CA265_RS20685 at 5117.643 kb on - strand, within CA265_RS20685 at 5117.742 kb on + strand, within CA265_RS20685 at 5117.797 kb on + strand, within CA265_RS20685 at 5117.816 kb on - strand, within CA265_RS20685 at 5117.853 kb on + strand, within CA265_RS20685 at 5117.854 kb on - strand, within CA265_RS20685 at 5117.982 kb on - strand at 5118.279 kb on + strand, within CA265_RS20690 at 5118.327 kb on + strand, within CA265_RS20690 at 5118.429 kb on + strand at 5118.451 kb on + strand, within CA265_RS20695 at 5118.452 kb on - strand, within CA265_RS20695 at 5118.464 kb on - strand, within CA265_RS20695 at 5118.489 kb on - strand, within CA265_RS20695 at 5118.590 kb on + strand, within CA265_RS20695 at 5118.724 kb on - strand at 5118.724 kb on - strand at 5118.739 kb on + strand at 5118.743 kb on - strand at 5118.755 kb on + strand at 5118.796 kb on + strand, within CA265_RS20700 at 5118.797 kb on - strand, within CA265_RS20700 at 5118.830 kb on + strand, within CA265_RS20700 at 5118.830 kb on + strand, within CA265_RS20700 at 5118.895 kb on + strand, within CA265_RS20700 at 5118.896 kb on - strand, within CA265_RS20700 at 5118.945 kb on - strand, within CA265_RS20700 at 5118.945 kb on - strand, within CA265_RS20700 at 5118.986 kb on - strand, within CA265_RS20700 at 5119.087 kb on - strand at 5119.091 kb on + strand at 5119.270 kb on + strand, within CA265_RS20705 at 5119.287 kb on + strand, within CA265_RS20705 at 5119.288 kb on - strand, within CA265_RS20705 at 5119.421 kb on + strand at 5119.421 kb on + strand at 5119.422 kb on - strand at 5119.446 kb on + strand, within CA265_RS20710 at 5119.446 kb on + strand, within CA265_RS20710 at 5119.446 kb on + strand, within CA265_RS20710 at 5119.446 kb on + strand, within CA265_RS20710 at 5119.447 kb on - strand, within CA265_RS20710 at 5119.544 kb on + strand, within CA265_RS20710 at 5119.544 kb on + strand, within CA265_RS20710 at 5119.545 kb on - strand, within CA265_RS20710 at 5119.545 kb on - strand, within CA265_RS20710 at 5119.647 kb on + strand, within CA265_RS20710 at 5119.647 kb on + strand, within CA265_RS20710 at 5119.648 kb on - strand, within CA265_RS20710 at 5119.648 kb on - strand, within CA265_RS20710 at 5119.668 kb on + strand, within CA265_RS20710 at 5119.675 kb on - strand, within CA265_RS20710 at 5119.675 kb on - strand, within CA265_RS20710 at 5119.675 kb on - strand, within CA265_RS20710
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,117,580 + CA265_RS20685 0.28 -0.7 5,117,580 + CA265_RS20685 0.28 -0.3 5,117,642 + CA265_RS20685 0.38 +1.4 5,117,642 + CA265_RS20685 0.38 -0.4 5,117,643 - CA265_RS20685 0.38 +0.3 5,117,742 + CA265_RS20685 0.53 -0.5 5,117,797 + CA265_RS20685 0.62 -1.4 5,117,816 - CA265_RS20685 0.65 +0.8 5,117,853 + CA265_RS20685 0.71 +1.8 5,117,854 - CA265_RS20685 0.71 -2.1 5,117,982 - +0.6 5,118,279 + CA265_RS20690 0.64 +0.1 5,118,327 + CA265_RS20690 0.77 -0.0 5,118,429 + -0.3 5,118,451 + CA265_RS20695 0.12 +0.6 5,118,452 - CA265_RS20695 0.12 +1.3 5,118,464 - CA265_RS20695 0.16 +1.0 5,118,489 - CA265_RS20695 0.23 +0.7 5,118,590 + CA265_RS20695 0.53 -0.4 5,118,724 - +1.0 5,118,724 - -0.4 5,118,739 + -1.4 5,118,743 - +0.6 5,118,755 + +0.0 5,118,796 + CA265_RS20700 0.13 +0.8 5,118,797 - CA265_RS20700 0.14 +0.1 5,118,830 + CA265_RS20700 0.24 +0.5 5,118,830 + CA265_RS20700 0.24 +0.0 5,118,895 + CA265_RS20700 0.46 -0.1 5,118,896 - CA265_RS20700 0.46 +0.2 5,118,945 - CA265_RS20700 0.62 -0.3 5,118,945 - CA265_RS20700 0.62 -0.1 5,118,986 - CA265_RS20700 0.76 +0.3 5,119,087 - +0.8 5,119,091 + +0.5 5,119,270 + CA265_RS20705 0.57 +0.9 5,119,287 + CA265_RS20705 0.62 +0.1 5,119,288 - CA265_RS20705 0.63 -0.2 5,119,421 + -1.6 5,119,421 + -1.6 5,119,422 - +0.6 5,119,446 + CA265_RS20710 0.14 -0.1 5,119,446 + CA265_RS20710 0.14 -1.9 5,119,446 + CA265_RS20710 0.14 -0.2 5,119,446 + CA265_RS20710 0.14 +0.3 5,119,447 - CA265_RS20710 0.14 +0.7 5,119,544 + CA265_RS20710 0.46 -1.3 5,119,544 + CA265_RS20710 0.46 -2.4 5,119,545 - CA265_RS20710 0.46 +0.0 5,119,545 - CA265_RS20710 0.46 -0.8 5,119,647 + CA265_RS20710 0.80 -0.3 5,119,647 + CA265_RS20710 0.80 -0.0 5,119,648 - CA265_RS20710 0.80 -0.0 5,119,648 - CA265_RS20710 0.80 -0.1 5,119,668 + CA265_RS20710 0.87 +0.9 5,119,675 - CA265_RS20710 0.89 +2.5 5,119,675 - CA265_RS20710 0.89 +0.9 5,119,675 - CA265_RS20710 0.89 +0.8
Or see this region's nucleotide sequence