Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09040 and CA265_RS09045 are separated by 345 nucleotides CA265_RS09045 and CA265_RS09050 are separated by 169 nucleotides CA265_RS09050 and CA265_RS09055 are separated by 12 nucleotides
CA265_RS09040: CA265_RS09040 - ribonuclease Z, at 2,252,679 to 2,253,584
_RS09040
CA265_RS09045: CA265_RS09045 - ABC transporter permease, at 2,253,930 to 2,254,883
_RS09045
CA265_RS09050: CA265_RS09050 - N-acetylmuramoyl-L-alanine amidase, at 2,255,053 to 2,255,829
_RS09050
CA265_RS09055: CA265_RS09055 - N-acetylmuramoyl-L-alanine amidase, at 2,255,842 to 2,256,705
_RS09055
Position (kb)
2253
2254
2255 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2252.944 kb on + strand, within CA265_RS09040 at 2252.962 kb on + strand, within CA265_RS09040 at 2252.963 kb on - strand, within CA265_RS09040 at 2253.076 kb on + strand, within CA265_RS09040 at 2253.114 kb on - strand, within CA265_RS09040 at 2253.116 kb on + strand, within CA265_RS09040 at 2253.116 kb on + strand, within CA265_RS09040 at 2253.192 kb on + strand, within CA265_RS09040 at 2253.196 kb on - strand, within CA265_RS09040 at 2253.198 kb on - strand, within CA265_RS09040 at 2253.262 kb on - strand, within CA265_RS09040 at 2253.263 kb on + strand, within CA265_RS09040 at 2253.263 kb on + strand, within CA265_RS09040 at 2253.298 kb on - strand, within CA265_RS09040 at 2253.428 kb on - strand, within CA265_RS09040 at 2253.546 kb on - strand at 2253.624 kb on + strand at 2253.656 kb on - strand at 2253.666 kb on - strand at 2253.703 kb on - strand at 2253.771 kb on + strand at 2253.787 kb on + strand at 2253.861 kb on + strand at 2253.867 kb on + strand at 2253.888 kb on - strand at 2253.932 kb on - strand at 2253.976 kb on + strand at 2254.056 kb on - strand, within CA265_RS09045 at 2254.069 kb on - strand, within CA265_RS09045 at 2254.071 kb on + strand, within CA265_RS09045 at 2254.078 kb on - strand, within CA265_RS09045 at 2254.098 kb on - strand, within CA265_RS09045 at 2254.120 kb on - strand, within CA265_RS09045 at 2254.145 kb on + strand, within CA265_RS09045 at 2254.240 kb on - strand, within CA265_RS09045 at 2254.350 kb on - strand, within CA265_RS09045 at 2254.358 kb on + strand, within CA265_RS09045 at 2254.450 kb on - strand, within CA265_RS09045 at 2254.477 kb on - strand, within CA265_RS09045 at 2254.518 kb on - strand, within CA265_RS09045 at 2254.518 kb on - strand, within CA265_RS09045 at 2254.547 kb on + strand, within CA265_RS09045 at 2254.552 kb on - strand, within CA265_RS09045 at 2254.559 kb on + strand, within CA265_RS09045 at 2254.560 kb on - strand, within CA265_RS09045 at 2254.560 kb on - strand, within CA265_RS09045 at 2254.574 kb on + strand, within CA265_RS09045 at 2254.607 kb on + strand, within CA265_RS09045 at 2254.608 kb on - strand, within CA265_RS09045 at 2254.627 kb on + strand, within CA265_RS09045 at 2254.639 kb on - strand, within CA265_RS09045 at 2254.756 kb on + strand, within CA265_RS09045 at 2254.767 kb on + strand, within CA265_RS09045 at 2254.768 kb on - strand, within CA265_RS09045 at 2254.768 kb on - strand, within CA265_RS09045 at 2254.773 kb on - strand, within CA265_RS09045 at 2254.828 kb on - strand at 2254.857 kb on + strand at 2254.893 kb on - strand at 2254.903 kb on + strand at 2254.904 kb on - strand at 2254.904 kb on - strand at 2254.904 kb on - strand at 2254.917 kb on + strand at 2254.974 kb on - strand at 2255.032 kb on - strand at 2255.100 kb on + strand at 2255.139 kb on + strand, within CA265_RS09050 at 2255.267 kb on + strand, within CA265_RS09050 at 2255.267 kb on + strand, within CA265_RS09050 at 2255.282 kb on - strand, within CA265_RS09050 at 2255.347 kb on - strand, within CA265_RS09050 at 2255.376 kb on - strand, within CA265_RS09050 at 2255.484 kb on + strand, within CA265_RS09050 at 2255.484 kb on + strand, within CA265_RS09050 at 2255.512 kb on - strand, within CA265_RS09050 at 2255.554 kb on + strand, within CA265_RS09050 at 2255.638 kb on + strand, within CA265_RS09050 at 2255.755 kb on - strand at 2255.804 kb on - strand at 2255.820 kb on + strand at 2255.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 2,252,944 + CA265_RS09040 0.29 -1.6 2,252,962 + CA265_RS09040 0.31 -0.6 2,252,963 - CA265_RS09040 0.31 +1.4 2,253,076 + CA265_RS09040 0.44 +1.4 2,253,114 - CA265_RS09040 0.48 -0.9 2,253,116 + CA265_RS09040 0.48 -0.1 2,253,116 + CA265_RS09040 0.48 +0.0 2,253,192 + CA265_RS09040 0.57 +0.7 2,253,196 - CA265_RS09040 0.57 -0.4 2,253,198 - CA265_RS09040 0.57 +0.6 2,253,262 - CA265_RS09040 0.64 +0.4 2,253,263 + CA265_RS09040 0.64 -0.5 2,253,263 + CA265_RS09040 0.64 +0.1 2,253,298 - CA265_RS09040 0.68 -0.7 2,253,428 - CA265_RS09040 0.83 +0.5 2,253,546 - -0.7 2,253,624 + +0.6 2,253,656 - +0.4 2,253,666 - +0.6 2,253,703 - -0.4 2,253,771 + -1.1 2,253,787 + +0.3 2,253,861 + -1.5 2,253,867 + +1.8 2,253,888 - -0.9 2,253,932 - -0.8 2,253,976 + +0.1 2,254,056 - CA265_RS09045 0.13 +0.9 2,254,069 - CA265_RS09045 0.15 +1.1 2,254,071 + CA265_RS09045 0.15 +0.1 2,254,078 - CA265_RS09045 0.16 +0.6 2,254,098 - CA265_RS09045 0.18 +1.6 2,254,120 - CA265_RS09045 0.20 -0.4 2,254,145 + CA265_RS09045 0.23 +0.7 2,254,240 - CA265_RS09045 0.32 +0.8 2,254,350 - CA265_RS09045 0.44 +0.1 2,254,358 + CA265_RS09045 0.45 +1.3 2,254,450 - CA265_RS09045 0.55 +0.1 2,254,477 - CA265_RS09045 0.57 -0.7 2,254,518 - CA265_RS09045 0.62 -0.5 2,254,518 - CA265_RS09045 0.62 +0.6 2,254,547 + CA265_RS09045 0.65 -1.8 2,254,552 - CA265_RS09045 0.65 -0.5 2,254,559 + CA265_RS09045 0.66 +0.1 2,254,560 - CA265_RS09045 0.66 +1.0 2,254,560 - CA265_RS09045 0.66 -0.6 2,254,574 + CA265_RS09045 0.68 +1.2 2,254,607 + CA265_RS09045 0.71 +0.3 2,254,608 - CA265_RS09045 0.71 +0.9 2,254,627 + CA265_RS09045 0.73 +1.2 2,254,639 - CA265_RS09045 0.74 +1.1 2,254,756 + CA265_RS09045 0.87 +1.9 2,254,767 + CA265_RS09045 0.88 +0.4 2,254,768 - CA265_RS09045 0.88 -0.1 2,254,768 - CA265_RS09045 0.88 +0.5 2,254,773 - CA265_RS09045 0.88 -0.7 2,254,828 - +0.7 2,254,857 + +1.9 2,254,893 - +0.1 2,254,903 + +0.3 2,254,904 - +0.3 2,254,904 - +0.1 2,254,904 - -0.0 2,254,917 + -0.7 2,254,974 - +0.1 2,255,032 - +0.4 2,255,100 + +0.3 2,255,139 + CA265_RS09050 0.11 +0.3 2,255,267 + CA265_RS09050 0.28 +0.3 2,255,267 + CA265_RS09050 0.28 +0.0 2,255,282 - CA265_RS09050 0.29 +0.3 2,255,347 - CA265_RS09050 0.38 +0.7 2,255,376 - CA265_RS09050 0.42 +0.2 2,255,484 + CA265_RS09050 0.55 +0.3 2,255,484 + CA265_RS09050 0.55 -0.2 2,255,512 - CA265_RS09050 0.59 +0.1 2,255,554 + CA265_RS09050 0.64 -0.5 2,255,638 + CA265_RS09050 0.75 +0.9 2,255,755 - +0.3 2,255,804 - -0.6 2,255,820 + +0.1 2,255,863 - +0.4
Or see this region's nucleotide sequence