Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23350 and EX31_RS25645 are separated by 0 nucleotides EX31_RS25645 and EX31_RS23355 are separated by 409 nucleotides EX31_RS23355 and EX31_RS23360 are separated by 181 nucleotides EX31_RS23360 and EX31_RS23365 are separated by 21 nucleotides
EX31_RS23350: EX31_RS23350 - potassium-transporting ATPase subunit KdpA, at 433,174 to 434,862
_RS23350
EX31_RS25645: EX31_RS25645 - K(+)-transporting ATPase subunit F, at 434,863 to 434,952
_RS25645
EX31_RS23355: EX31_RS23355 - YbfA family protein, at 435,362 to 435,568
_RS23355
EX31_RS23360: EX31_RS23360 - DUF1722 domain-containing protein, at 435,750 to 436,703
_RS23360
EX31_RS23365: EX31_RS23365 - deoxyribodipyrimidine photo-lyase, at 436,725 to 438,155
_RS23365
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 434.790 kb on + strand at 434.791 kb on - strand at 434.827 kb on - strand at 434.827 kb on - strand at 434.827 kb on - strand at 434.827 kb on - strand at 434.842 kb on + strand at 434.891 kb on + strand, within EX31_RS25645 at 434.923 kb on + strand, within EX31_RS25645 at 434.939 kb on - strand, within EX31_RS25645 at 434.977 kb on + strand at 435.048 kb on + strand at 435.098 kb on + strand at 435.171 kb on + strand at 435.171 kb on + strand at 435.171 kb on + strand at 435.172 kb on - strand at 435.172 kb on - strand at 435.195 kb on + strand at 435.196 kb on - strand at 435.200 kb on - strand at 435.306 kb on + strand at 435.321 kb on - strand at 435.321 kb on - strand at 435.321 kb on - strand at 435.321 kb on - strand at 435.321 kb on - strand at 435.321 kb on - strand at 435.371 kb on + strand at 435.372 kb on - strand at 435.380 kb on + strand at 435.381 kb on - strand at 435.381 kb on - strand at 435.396 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.485 kb on + strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.486 kb on - strand, within EX31_RS23355 at 435.489 kb on + strand, within EX31_RS23355 at 435.489 kb on + strand, within EX31_RS23355 at 435.490 kb on - strand, within EX31_RS23355 at 435.490 kb on - strand, within EX31_RS23355 at 435.564 kb on - strand at 435.571 kb on + strand at 435.572 kb on - strand at 435.628 kb on + strand at 435.650 kb on - strand at 435.667 kb on + strand at 435.667 kb on + strand at 435.668 kb on - strand at 435.679 kb on + strand at 435.685 kb on + strand at 435.685 kb on + strand at 435.685 kb on + strand at 435.685 kb on + strand at 435.686 kb on - strand at 435.686 kb on - strand at 435.687 kb on + strand at 435.687 kb on + strand at 435.687 kb on + strand at 435.687 kb on + strand at 435.688 kb on - strand at 435.688 kb on - strand at 435.688 kb on - strand at 435.688 kb on - strand at 435.688 kb on - strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.691 kb on + strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.692 kb on - strand at 435.718 kb on + strand at 435.718 kb on + strand at 435.719 kb on - strand at 435.821 kb on + strand at 435.858 kb on + strand, within EX31_RS23360 at 435.858 kb on + strand, within EX31_RS23360 at 435.858 kb on + strand, within EX31_RS23360 at 435.858 kb on + strand, within EX31_RS23360 at 435.859 kb on - strand, within EX31_RS23360 at 435.859 kb on - strand, within EX31_RS23360 at 435.859 kb on - strand, within EX31_RS23360 at 435.895 kb on + strand, within EX31_RS23360 at 435.901 kb on + strand, within EX31_RS23360 at 436.005 kb on + strand, within EX31_RS23360 at 436.005 kb on + strand, within EX31_RS23360 at 436.005 kb on + strand, within EX31_RS23360 at 436.005 kb on + strand, within EX31_RS23360 at 436.047 kb on + strand, within EX31_RS23360 at 436.047 kb on + strand, within EX31_RS23360 at 436.047 kb on + strand, within EX31_RS23360 at 436.047 kb on + strand, within EX31_RS23360 at 436.048 kb on - strand, within EX31_RS23360 at 436.048 kb on - strand, within EX31_RS23360 at 436.048 kb on - strand, within EX31_RS23360 at 436.049 kb on + strand, within EX31_RS23360 at 436.049 kb on + strand, within EX31_RS23360 at 436.049 kb on + strand, within EX31_RS23360 at 436.050 kb on - strand, within EX31_RS23360 at 436.050 kb on - strand, within EX31_RS23360 at 436.050 kb on - strand, within EX31_RS23360 at 436.098 kb on + strand, within EX31_RS23360 at 436.099 kb on - strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.143 kb on + strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.144 kb on - strand, within EX31_RS23360 at 436.146 kb on - strand, within EX31_RS23360 at 436.146 kb on - strand, within EX31_RS23360 at 436.319 kb on + strand, within EX31_RS23360 at 436.319 kb on + strand, within EX31_RS23360 at 436.319 kb on + strand, within EX31_RS23360 at 436.410 kb on + strand, within EX31_RS23360 at 436.411 kb on - strand, within EX31_RS23360 at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.614 kb on + strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.615 kb on - strand at 436.626 kb on + strand at 436.638 kb on + strand at 436.639 kb on - strand at 436.639 kb on - strand at 436.656 kb on + strand at 436.657 kb on - strand at 436.657 kb on - strand at 436.681 kb on + strand at 436.701 kb on + strand at 436.701 kb on + strand at 436.701 kb on + strand at 436.702 kb on - strand at 436.704 kb on + strand at 436.704 kb on + strand at 436.704 kb on + strand at 436.705 kb on - strand at 436.710 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.712 kb on + strand at 436.713 kb on - strand at 436.713 kb on - strand at 436.713 kb on - strand at 436.724 kb on + strand at 436.724 kb on + strand at 436.724 kb on + strand at 436.725 kb on - strand at 436.725 kb on - strand at 436.738 kb on + strand at 436.738 kb on + strand at 436.739 kb on - strand at 436.817 kb on + strand at 436.825 kb on + strand at 436.825 kb on + strand at 436.826 kb on - strand at 436.886 kb on + strand, within EX31_RS23365 at 436.887 kb on - strand, within EX31_RS23365 at 436.887 kb on - strand, within EX31_RS23365 at 436.887 kb on - strand, within EX31_RS23365 at 436.890 kb on + strand, within EX31_RS23365 at 437.034 kb on - strand, within EX31_RS23365 at 437.041 kb on - strand, within EX31_RS23365 at 437.169 kb on + strand, within EX31_RS23365 at 437.169 kb on + strand, within EX31_RS23365 at 437.169 kb on + strand, within EX31_RS23365 at 437.169 kb on + strand, within EX31_RS23365 at 437.170 kb on - strand, within EX31_RS23365 at 437.178 kb on + strand, within EX31_RS23365 at 437.178 kb on + strand, within EX31_RS23365 at 437.179 kb on - strand, within EX31_RS23365 at 437.179 kb on - strand, within EX31_RS23365 at 437.180 kb on + strand, within EX31_RS23365 at 437.180 kb on + strand, within EX31_RS23365 at 437.181 kb on - strand, within EX31_RS23365 at 437.448 kb on + strand, within EX31_RS23365 at 437.449 kb on - strand, within EX31_RS23365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 434,790 + -1.7 434,791 - +2.9 434,827 - +0.1 434,827 - -0.7 434,827 - +0.8 434,827 - +1.9 434,842 + +0.6 434,891 + EX31_RS25645 0.31 -0.1 434,923 + EX31_RS25645 0.67 +1.3 434,939 - EX31_RS25645 0.84 +2.9 434,977 + -0.1 435,048 + +0.9 435,098 + +0.1 435,171 + +1.3 435,171 + +0.9 435,171 + -0.4 435,172 - +0.7 435,172 - +1.4 435,195 + +1.9 435,196 - +0.3 435,200 - +0.2 435,306 + -0.5 435,321 - +0.3 435,321 - -0.2 435,321 - -0.2 435,321 - +0.7 435,321 - -1.4 435,321 - +0.5 435,371 + -0.5 435,372 - -0.1 435,380 + +0.9 435,381 - +1.3 435,381 - -0.9 435,396 + EX31_RS23355 0.16 +0.3 435,485 + EX31_RS23355 0.59 +1.6 435,485 + EX31_RS23355 0.59 -1.2 435,485 + EX31_RS23355 0.59 +1.6 435,485 + EX31_RS23355 0.59 -0.1 435,485 + EX31_RS23355 0.59 +0.1 435,485 + EX31_RS23355 0.59 -0.3 435,485 + EX31_RS23355 0.59 +0.8 435,486 - EX31_RS23355 0.60 +0.9 435,486 - EX31_RS23355 0.60 +0.6 435,486 - EX31_RS23355 0.60 +0.3 435,486 - EX31_RS23355 0.60 +1.4 435,486 - EX31_RS23355 0.60 -0.8 435,486 - EX31_RS23355 0.60 -0.7 435,489 + EX31_RS23355 0.61 -0.4 435,489 + EX31_RS23355 0.61 +1.5 435,490 - EX31_RS23355 0.62 -0.1 435,490 - EX31_RS23355 0.62 +1.5 435,564 - -0.3 435,571 + -0.2 435,572 - -1.2 435,628 + +0.8 435,650 - +0.3 435,667 + +1.3 435,667 + -2.5 435,668 - +1.4 435,679 + +1.0 435,685 + +2.3 435,685 + -0.1 435,685 + +1.5 435,685 + +0.8 435,686 - -0.4 435,686 - -0.8 435,687 + -1.2 435,687 + -0.7 435,687 + +0.6 435,687 + +0.9 435,688 - +1.1 435,688 - +1.3 435,688 - -1.4 435,688 - +0.1 435,688 - +0.3 435,691 + +0.1 435,691 + +1.1 435,691 + -0.4 435,691 + +0.3 435,691 + +2.5 435,691 + +0.0 435,691 + -0.4 435,691 + +0.9 435,691 + -1.1 435,691 + +1.3 435,691 + -0.3 435,691 + +0.9 435,692 - +0.7 435,692 - +0.3 435,692 - -0.4 435,692 - -2.1 435,692 - +1.9 435,692 - -0.3 435,692 - -0.7 435,692 - -0.7 435,692 - +0.4 435,718 + -0.5 435,718 + +0.3 435,719 - +0.3 435,821 + -0.5 435,858 + EX31_RS23360 0.11 +3.5 435,858 + EX31_RS23360 0.11 +1.9 435,858 + EX31_RS23360 0.11 +1.2 435,858 + EX31_RS23360 0.11 +0.9 435,859 - EX31_RS23360 0.11 +1.1 435,859 - EX31_RS23360 0.11 +0.5 435,859 - EX31_RS23360 0.11 +1.4 435,895 + EX31_RS23360 0.15 +0.3 435,901 + EX31_RS23360 0.16 +0.7 436,005 + EX31_RS23360 0.27 +0.6 436,005 + EX31_RS23360 0.27 -0.4 436,005 + EX31_RS23360 0.27 +0.5 436,005 + EX31_RS23360 0.27 -0.9 436,047 + EX31_RS23360 0.31 -0.9 436,047 + EX31_RS23360 0.31 -0.3 436,047 + EX31_RS23360 0.31 -0.9 436,047 + EX31_RS23360 0.31 -0.4 436,048 - EX31_RS23360 0.31 +0.3 436,048 - EX31_RS23360 0.31 -0.4 436,048 - EX31_RS23360 0.31 +1.9 436,049 + EX31_RS23360 0.31 +0.0 436,049 + EX31_RS23360 0.31 +0.1 436,049 + EX31_RS23360 0.31 +1.0 436,050 - EX31_RS23360 0.31 +0.2 436,050 - EX31_RS23360 0.31 +1.2 436,050 - EX31_RS23360 0.31 +1.4 436,098 + EX31_RS23360 0.36 +0.9 436,099 - EX31_RS23360 0.37 +1.3 436,143 + EX31_RS23360 0.41 -0.1 436,143 + EX31_RS23360 0.41 +0.3 436,143 + EX31_RS23360 0.41 -0.1 436,143 + EX31_RS23360 0.41 +0.6 436,143 + EX31_RS23360 0.41 -0.3 436,143 + EX31_RS23360 0.41 +2.7 436,143 + EX31_RS23360 0.41 +1.3 436,143 + EX31_RS23360 0.41 -0.4 436,143 + EX31_RS23360 0.41 +1.0 436,143 + EX31_RS23360 0.41 -1.9 436,143 + EX31_RS23360 0.41 +0.9 436,143 + EX31_RS23360 0.41 +0.9 436,143 + EX31_RS23360 0.41 +0.1 436,143 + EX31_RS23360 0.41 -0.1 436,143 + EX31_RS23360 0.41 +0.7 436,144 - EX31_RS23360 0.41 +1.3 436,144 - EX31_RS23360 0.41 +0.1 436,144 - EX31_RS23360 0.41 +0.3 436,144 - EX31_RS23360 0.41 +0.4 436,144 - EX31_RS23360 0.41 -0.0 436,144 - EX31_RS23360 0.41 +1.0 436,144 - EX31_RS23360 0.41 -0.3 436,146 - EX31_RS23360 0.42 -0.2 436,146 - EX31_RS23360 0.42 -1.1 436,319 + EX31_RS23360 0.60 -1.3 436,319 + EX31_RS23360 0.60 -0.4 436,319 + EX31_RS23360 0.60 +1.4 436,410 + EX31_RS23360 0.69 +0.4 436,411 - EX31_RS23360 0.69 +0.5 436,614 + +0.1 436,614 + +0.8 436,614 + +0.2 436,614 + +0.0 436,614 + +0.4 436,614 + +1.5 436,614 + +0.6 436,614 + +1.1 436,614 + -0.9 436,614 + -1.8 436,614 + +0.7 436,614 + -0.7 436,614 + -0.7 436,614 + -0.8 436,614 + +0.8 436,614 + +0.6 436,615 - +0.7 436,615 - -1.5 436,615 - -1.1 436,615 - -0.4 436,615 - +0.9 436,615 - +1.1 436,615 - -0.2 436,626 + +0.1 436,638 + +2.3 436,639 - +0.4 436,639 - +0.1 436,656 + +0.2 436,657 - +0.7 436,657 - +0.3 436,681 + +0.9 436,701 + +0.2 436,701 + +0.3 436,701 + +1.4 436,702 - -0.4 436,704 + -0.4 436,704 + +0.3 436,704 + -1.3 436,705 - +0.0 436,710 + -0.5 436,712 + -0.4 436,712 + -0.1 436,712 + -0.7 436,712 + +0.0 436,712 + +0.8 436,712 + -1.5 436,712 + -0.1 436,713 - -2.2 436,713 - +1.6 436,713 - -0.7 436,724 + -1.2 436,724 + +0.7 436,724 + -0.6 436,725 - +1.0 436,725 - -1.8 436,738 + -1.4 436,738 + +1.2 436,739 - -0.1 436,817 + -0.1 436,825 + -0.5 436,825 + -0.3 436,826 - -0.3 436,886 + EX31_RS23365 0.11 +0.5 436,887 - EX31_RS23365 0.11 +0.7 436,887 - EX31_RS23365 0.11 -1.1 436,887 - EX31_RS23365 0.11 -0.6 436,890 + EX31_RS23365 0.12 +0.7 437,034 - EX31_RS23365 0.22 -0.6 437,041 - EX31_RS23365 0.22 -0.2 437,169 + EX31_RS23365 0.31 -0.1 437,169 + EX31_RS23365 0.31 +0.9 437,169 + EX31_RS23365 0.31 -0.4 437,169 + EX31_RS23365 0.31 +0.2 437,170 - EX31_RS23365 0.31 +0.7 437,178 + EX31_RS23365 0.32 -0.8 437,178 + EX31_RS23365 0.32 -1.8 437,179 - EX31_RS23365 0.32 -1.4 437,179 - EX31_RS23365 0.32 -0.3 437,180 + EX31_RS23365 0.32 +1.7 437,180 + EX31_RS23365 0.32 +1.9 437,181 - EX31_RS23365 0.32 +1.5 437,448 + EX31_RS23365 0.51 +0.8 437,449 - EX31_RS23365 0.51 +1.2
Or see this region's nucleotide sequence