Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23035 and EX31_RS23040 are separated by 9 nucleotides EX31_RS23040 and EX31_RS23045 are separated by 459 nucleotides
EX31_RS23035: EX31_RS23035 - multidrug effflux MFS transporter, at 369,348 to 370,535
_RS23035
EX31_RS23040: EX31_RS23040 - helix-turn-helix transcriptional regulator, at 370,545 to 371,201
_RS23040
EX31_RS23045: EX31_RS23045 - fluoride efflux transporter CrcB, at 371,661 to 372,044
_RS23045
Position (kb) 370
371
372 Strain fitness (log2 ratio) -2
-1
0
1
2 at 369.566 kb on + strand, within EX31_RS23035 at 369.567 kb on - strand, within EX31_RS23035 at 369.606 kb on + strand, within EX31_RS23035 at 369.802 kb on + strand, within EX31_RS23035 at 369.803 kb on - strand, within EX31_RS23035 at 369.803 kb on - strand, within EX31_RS23035 at 369.837 kb on - strand, within EX31_RS23035 at 369.838 kb on + strand, within EX31_RS23035 at 369.839 kb on - strand, within EX31_RS23035 at 369.839 kb on - strand, within EX31_RS23035 at 369.857 kb on + strand, within EX31_RS23035 at 369.858 kb on - strand, within EX31_RS23035 at 369.858 kb on - strand, within EX31_RS23035 at 369.861 kb on - strand, within EX31_RS23035 at 369.861 kb on - strand, within EX31_RS23035 at 370.005 kb on + strand, within EX31_RS23035 at 370.006 kb on - strand, within EX31_RS23035 at 370.006 kb on - strand, within EX31_RS23035 at 370.006 kb on - strand, within EX31_RS23035 at 370.154 kb on - strand, within EX31_RS23035 at 370.154 kb on - strand, within EX31_RS23035 at 370.344 kb on + strand, within EX31_RS23035 at 370.345 kb on - strand, within EX31_RS23035 at 370.386 kb on + strand, within EX31_RS23035 at 370.410 kb on - strand, within EX31_RS23035 at 370.410 kb on - strand, within EX31_RS23035 at 370.410 kb on - strand, within EX31_RS23035 at 370.410 kb on - strand, within EX31_RS23035 at 370.410 kb on - strand, within EX31_RS23035 at 370.443 kb on + strand at 370.444 kb on - strand at 370.448 kb on - strand at 370.450 kb on - strand at 370.462 kb on - strand at 370.538 kb on + strand at 370.546 kb on + strand at 370.567 kb on - strand at 370.595 kb on - strand at 370.603 kb on + strand at 370.603 kb on + strand at 370.603 kb on + strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.604 kb on - strand at 370.605 kb on + strand at 370.890 kb on - strand, within EX31_RS23040 at 370.891 kb on + strand, within EX31_RS23040 at 370.892 kb on - strand, within EX31_RS23040 at 370.953 kb on - strand, within EX31_RS23040 at 370.993 kb on - strand, within EX31_RS23040 at 371.007 kb on - strand, within EX31_RS23040 at 371.039 kb on - strand, within EX31_RS23040 at 371.039 kb on - strand, within EX31_RS23040 at 371.039 kb on - strand, within EX31_RS23040 at 371.046 kb on + strand, within EX31_RS23040 at 371.047 kb on - strand, within EX31_RS23040 at 371.177 kb on + strand at 371.227 kb on + strand at 371.261 kb on + strand at 371.279 kb on + strand at 371.279 kb on + strand at 371.280 kb on - strand at 371.286 kb on - strand at 371.288 kb on + strand at 371.289 kb on - strand at 371.289 kb on - strand at 371.328 kb on + strand at 371.329 kb on - strand at 371.329 kb on - strand at 371.414 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.423 kb on + strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.424 kb on - strand at 371.510 kb on + strand at 371.510 kb on + strand at 371.510 kb on + strand at 371.511 kb on - strand at 371.511 kb on - strand at 371.511 kb on - strand at 371.511 kb on - strand at 371.511 kb on - strand at 371.542 kb on + strand at 371.543 kb on - strand at 371.545 kb on + strand at 371.545 kb on + strand at 371.545 kb on + strand at 371.546 kb on - strand at 371.546 kb on - strand at 371.546 kb on - strand at 371.546 kb on - strand at 371.555 kb on + strand at 371.555 kb on + strand at 371.556 kb on - strand at 371.556 kb on - strand at 371.556 kb on - strand at 371.572 kb on + strand at 371.656 kb on + strand at 371.656 kb on + strand at 371.657 kb on - strand at 371.657 kb on - strand at 371.690 kb on + strand at 371.690 kb on + strand at 371.691 kb on - strand at 371.691 kb on - strand at 371.700 kb on + strand, within EX31_RS23045 at 371.742 kb on + strand, within EX31_RS23045 at 371.742 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 372.005 kb on + strand, within EX31_RS23045 at 372.030 kb on + strand at 372.031 kb on - strand at 372.031 kb on - strand at 372.031 kb on - strand at 372.038 kb on + strand at 372.038 kb on + strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.044 kb on + strand at 372.045 kb on - strand at 372.045 kb on - strand at 372.045 kb on - strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.063 kb on - strand at 372.095 kb on + strand at 372.135 kb on + strand at 372.176 kb on + strand at 372.177 kb on - strand at 372.177 kb on - strand at 372.177 kb on - strand at 372.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 369,566 + EX31_RS23035 0.18 -2.2 369,567 - EX31_RS23035 0.18 +0.4 369,606 + EX31_RS23035 0.22 +0.9 369,802 + EX31_RS23035 0.38 -0.1 369,803 - EX31_RS23035 0.38 -0.7 369,803 - EX31_RS23035 0.38 +0.8 369,837 - EX31_RS23035 0.41 +0.1 369,838 + EX31_RS23035 0.41 -0.5 369,839 - EX31_RS23035 0.41 +1.3 369,839 - EX31_RS23035 0.41 -0.1 369,857 + EX31_RS23035 0.43 +1.0 369,858 - EX31_RS23035 0.43 -1.5 369,858 - EX31_RS23035 0.43 +0.4 369,861 - EX31_RS23035 0.43 +1.1 369,861 - EX31_RS23035 0.43 -0.8 370,005 + EX31_RS23035 0.55 +1.2 370,006 - EX31_RS23035 0.55 -0.2 370,006 - EX31_RS23035 0.55 +0.9 370,006 - EX31_RS23035 0.55 +0.2 370,154 - EX31_RS23035 0.68 +1.4 370,154 - EX31_RS23035 0.68 +0.4 370,344 + EX31_RS23035 0.84 +1.1 370,345 - EX31_RS23035 0.84 +0.8 370,386 + EX31_RS23035 0.87 +1.5 370,410 - EX31_RS23035 0.89 -1.4 370,410 - EX31_RS23035 0.89 -0.4 370,410 - EX31_RS23035 0.89 +0.9 370,410 - EX31_RS23035 0.89 -0.3 370,410 - EX31_RS23035 0.89 -0.6 370,443 + +0.1 370,444 - -0.1 370,448 - -0.1 370,450 - +0.7 370,462 - +0.1 370,538 + -0.2 370,546 + +0.3 370,567 - +0.2 370,595 - -0.0 370,603 + -0.2 370,603 + +0.5 370,603 + +0.2 370,604 - +0.8 370,604 - +1.3 370,604 - +0.2 370,604 - -0.1 370,604 - -0.3 370,604 - +0.5 370,604 - -1.5 370,604 - +1.0 370,604 - -0.5 370,605 + +1.5 370,890 - EX31_RS23040 0.53 -0.3 370,891 + EX31_RS23040 0.53 +0.4 370,892 - EX31_RS23040 0.53 -0.2 370,953 - EX31_RS23040 0.62 +0.3 370,993 - EX31_RS23040 0.68 +0.2 371,007 - EX31_RS23040 0.70 -0.4 371,039 - EX31_RS23040 0.75 +0.4 371,039 - EX31_RS23040 0.75 -0.1 371,039 - EX31_RS23040 0.75 -0.4 371,046 + EX31_RS23040 0.76 +0.4 371,047 - EX31_RS23040 0.76 -0.6 371,177 + +1.1 371,227 + -0.1 371,261 + +0.5 371,279 + -1.0 371,279 + +0.8 371,280 - -1.1 371,286 - -1.2 371,288 + +1.1 371,289 - -0.2 371,289 - +1.9 371,328 + +1.5 371,329 - -0.1 371,329 - +0.9 371,414 + +0.1 371,423 + +1.3 371,423 + +1.0 371,423 + +0.1 371,423 + -0.7 371,423 + -1.1 371,423 + +0.3 371,423 + -1.8 371,423 + -0.7 371,423 + +0.6 371,424 - -0.4 371,424 - +0.5 371,424 - +1.3 371,424 - -0.3 371,424 - +0.8 371,424 - -0.9 371,424 - -0.7 371,510 + +0.5 371,510 + +0.3 371,510 + -0.9 371,511 - +0.7 371,511 - +0.8 371,511 - -0.3 371,511 - -0.3 371,511 - +1.9 371,542 + -0.4 371,543 - +0.3 371,545 + -0.4 371,545 + -0.9 371,545 + +0.4 371,546 - +0.1 371,546 - +0.4 371,546 - +0.8 371,546 - +1.7 371,555 + +0.3 371,555 + -1.2 371,556 - -0.1 371,556 - +0.5 371,556 - -0.4 371,572 + +0.3 371,656 + +0.9 371,656 + +0.9 371,657 - +0.7 371,657 - -1.7 371,690 + -0.2 371,690 + -0.1 371,691 - -0.4 371,691 - +0.7 371,700 + EX31_RS23045 0.10 -1.9 371,742 + EX31_RS23045 0.21 +0.1 371,742 + EX31_RS23045 0.21 +1.6 371,772 + EX31_RS23045 0.29 -0.5 371,772 + EX31_RS23045 0.29 -0.1 371,772 + EX31_RS23045 0.29 +0.2 371,772 + EX31_RS23045 0.29 -0.8 371,772 + EX31_RS23045 0.29 +0.6 371,773 - EX31_RS23045 0.29 +1.9 371,773 - EX31_RS23045 0.29 -2.7 371,773 - EX31_RS23045 0.29 +1.3 371,779 + EX31_RS23045 0.31 +0.5 371,779 + EX31_RS23045 0.31 +0.3 371,779 + EX31_RS23045 0.31 -0.2 371,779 + EX31_RS23045 0.31 -0.2 371,779 + EX31_RS23045 0.31 +0.3 371,779 + EX31_RS23045 0.31 +0.9 371,779 + EX31_RS23045 0.31 +0.7 371,779 + EX31_RS23045 0.31 +0.1 371,780 - EX31_RS23045 0.31 +0.9 371,780 - EX31_RS23045 0.31 -1.9 371,780 - EX31_RS23045 0.31 +0.2 371,780 - EX31_RS23045 0.31 -0.2 371,780 - EX31_RS23045 0.31 +0.4 371,780 - EX31_RS23045 0.31 +0.3 371,780 - EX31_RS23045 0.31 +0.9 371,983 + EX31_RS23045 0.84 -0.2 371,983 + EX31_RS23045 0.84 +0.1 371,983 + EX31_RS23045 0.84 -1.2 371,984 - EX31_RS23045 0.84 +0.6 371,984 - EX31_RS23045 0.84 +0.3 371,984 - EX31_RS23045 0.84 -0.6 371,984 - EX31_RS23045 0.84 -0.1 371,984 - EX31_RS23045 0.84 -0.6 372,005 + EX31_RS23045 0.90 -1.9 372,030 + +0.3 372,031 - +0.8 372,031 - +0.4 372,031 - -0.2 372,038 + -0.2 372,038 + +0.6 372,039 - -0.4 372,039 - -1.9 372,039 - +0.1 372,039 - +0.6 372,039 - -0.3 372,044 + -0.1 372,045 - -0.1 372,045 - +0.4 372,045 - -0.9 372,050 + -0.9 372,050 + -0.8 372,050 + -0.4 372,050 + -1.5 372,050 + +0.5 372,051 - +0.7 372,051 - -0.4 372,051 - +0.3 372,051 - +0.6 372,051 - +0.5 372,051 - +0.8 372,051 - +0.1 372,063 - +0.6 372,095 + -1.4 372,135 + +0.5 372,176 + -0.6 372,177 - -1.5 372,177 - +0.9 372,177 - +1.1 372,177 - +0.8
Or see this region's nucleotide sequence