Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21130 and EX31_RS21135 are separated by 36 nucleotides EX31_RS21135 and EX31_RS21140 overlap by 4 nucleotides EX31_RS21140 and EX31_RS21145 are separated by 54 nucleotides
EX31_RS21130: EX31_RS21130 - gamma-glutamyltransferase, at 869,390 to 870,976
_RS21130
EX31_RS21135: EX31_RS21135 - oxalurate catabolism protein HpxX, at 871,013 to 871,207
_RS21135
EX31_RS21140: EX31_RS21140 - AtzE family amidohydrolase, at 871,204 to 872,607
_RS21140
EX31_RS21145: EX31_RS21145 - oxalurate catabolism protein HpxZ, at 872,662 to 873,045
_RS21145
Position (kb) 871
872
873 Strain fitness (log2 ratio) -2
-1
0
1
2 at 870.283 kb on + strand, within EX31_RS21130 at 870.283 kb on + strand, within EX31_RS21130 at 870.339 kb on - strand, within EX31_RS21130 at 870.339 kb on - strand, within EX31_RS21130 at 870.397 kb on + strand, within EX31_RS21130 at 870.397 kb on + strand, within EX31_RS21130 at 870.398 kb on - strand, within EX31_RS21130 at 870.398 kb on - strand, within EX31_RS21130 at 870.460 kb on + strand, within EX31_RS21130 at 870.473 kb on + strand, within EX31_RS21130 at 870.473 kb on + strand, within EX31_RS21130 at 870.474 kb on - strand, within EX31_RS21130 at 870.564 kb on + strand, within EX31_RS21130 at 870.565 kb on - strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.625 kb on - strand, within EX31_RS21130 at 870.625 kb on - strand, within EX31_RS21130 at 870.626 kb on + strand, within EX31_RS21130 at 870.626 kb on + strand, within EX31_RS21130 at 870.627 kb on - strand, within EX31_RS21130 at 870.627 kb on - strand, within EX31_RS21130 at 870.648 kb on - strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.766 kb on + strand, within EX31_RS21130 at 870.767 kb on - strand, within EX31_RS21130 at 870.953 kb on - strand at 870.989 kb on + strand at 870.989 kb on + strand at 871.021 kb on + strand at 871.022 kb on - strand at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.051 kb on + strand, within EX31_RS21135 at 871.051 kb on + strand, within EX31_RS21135 at 871.161 kb on + strand, within EX31_RS21135 at 871.161 kb on + strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.218 kb on + strand at 871.218 kb on + strand at 871.218 kb on + strand at 871.218 kb on + strand at 871.219 kb on - strand at 871.219 kb on - strand at 871.238 kb on + strand at 871.238 kb on + strand at 871.238 kb on + strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.406 kb on + strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.588 kb on + strand, within EX31_RS21140 at 871.621 kb on + strand, within EX31_RS21140 at 871.622 kb on - strand, within EX31_RS21140 at 871.732 kb on - strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.889 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140 at 872.009 kb on - strand, within EX31_RS21140 at 872.048 kb on + strand, within EX31_RS21140 at 872.048 kb on + strand, within EX31_RS21140 at 872.049 kb on - strand, within EX31_RS21140 at 872.128 kb on - strand, within EX31_RS21140 at 872.158 kb on + strand, within EX31_RS21140 at 872.158 kb on + strand, within EX31_RS21140 at 872.159 kb on - strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.409 kb on + strand, within EX31_RS21140 at 872.410 kb on - strand, within EX31_RS21140 at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.662 kb on - strand at 872.662 kb on - strand at 872.676 kb on + strand at 872.677 kb on - strand at 872.719 kb on + strand, within EX31_RS21145 at 872.719 kb on + strand, within EX31_RS21145 at 872.720 kb on - strand, within EX31_RS21145 at 872.822 kb on - strand, within EX31_RS21145 at 872.873 kb on + strand, within EX31_RS21145 at 872.874 kb on - strand, within EX31_RS21145 at 872.898 kb on + strand, within EX31_RS21145 at 873.168 kb on + strand at 873.182 kb on + strand at 873.182 kb on + strand at 873.249 kb on + strand at 873.249 kb on + strand at 873.250 kb on - strand at 873.288 kb on + strand at 873.289 kb on - strand at 873.289 kb on - strand at 873.436 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.551 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 870,283 + EX31_RS21130 0.56 +0.4 870,283 + EX31_RS21130 0.56 +0.2 870,339 - EX31_RS21130 0.60 -0.1 870,339 - EX31_RS21130 0.60 -0.8 870,397 + EX31_RS21130 0.63 +0.2 870,397 + EX31_RS21130 0.63 +0.3 870,398 - EX31_RS21130 0.64 -1.0 870,398 - EX31_RS21130 0.64 +0.2 870,460 + EX31_RS21130 0.67 -1.3 870,473 + EX31_RS21130 0.68 +0.7 870,473 + EX31_RS21130 0.68 +0.2 870,474 - EX31_RS21130 0.68 +1.1 870,564 + EX31_RS21130 0.74 -1.5 870,565 - EX31_RS21130 0.74 -0.5 870,624 + EX31_RS21130 0.78 -0.6 870,624 + EX31_RS21130 0.78 +0.0 870,624 + EX31_RS21130 0.78 +1.6 870,625 - EX31_RS21130 0.78 -0.5 870,625 - EX31_RS21130 0.78 -0.1 870,626 + EX31_RS21130 0.78 -0.9 870,626 + EX31_RS21130 0.78 -0.8 870,627 - EX31_RS21130 0.78 +0.7 870,627 - EX31_RS21130 0.78 -0.8 870,648 - EX31_RS21130 0.79 -0.3 870,708 + EX31_RS21130 0.83 +0.2 870,708 + EX31_RS21130 0.83 -0.3 870,708 + EX31_RS21130 0.83 -1.6 870,708 + EX31_RS21130 0.83 +0.2 870,708 + EX31_RS21130 0.83 -1.3 870,708 + EX31_RS21130 0.83 +0.7 870,708 + EX31_RS21130 0.83 -0.8 870,708 + EX31_RS21130 0.83 -0.3 870,708 + EX31_RS21130 0.83 -0.0 870,708 + EX31_RS21130 0.83 -0.1 870,708 + EX31_RS21130 0.83 +0.0 870,708 + EX31_RS21130 0.83 +0.4 870,708 + EX31_RS21130 0.83 -0.8 870,708 + EX31_RS21130 0.83 +1.2 870,708 + EX31_RS21130 0.83 +0.4 870,708 + EX31_RS21130 0.83 +0.3 870,709 - EX31_RS21130 0.83 +0.3 870,709 - EX31_RS21130 0.83 +0.1 870,709 - EX31_RS21130 0.83 -0.2 870,709 - EX31_RS21130 0.83 -0.1 870,709 - EX31_RS21130 0.83 +0.5 870,709 - EX31_RS21130 0.83 +1.2 870,709 - EX31_RS21130 0.83 -0.5 870,709 - EX31_RS21130 0.83 +0.1 870,709 - EX31_RS21130 0.83 +0.4 870,709 - EX31_RS21130 0.83 -1.3 870,709 - EX31_RS21130 0.83 +0.2 870,709 - EX31_RS21130 0.83 -1.2 870,709 - EX31_RS21130 0.83 -0.5 870,709 - EX31_RS21130 0.83 +0.7 870,766 + EX31_RS21130 0.87 -2.6 870,767 - EX31_RS21130 0.87 +0.2 870,953 - +0.8 870,989 + -2.1 870,989 + -0.6 871,021 + -0.0 871,022 - +0.4 871,049 + EX31_RS21135 0.18 -0.1 871,049 + EX31_RS21135 0.18 +0.1 871,049 + EX31_RS21135 0.18 -0.6 871,049 + EX31_RS21135 0.18 +0.3 871,050 - EX31_RS21135 0.19 +0.3 871,050 - EX31_RS21135 0.19 -0.5 871,050 - EX31_RS21135 0.19 -0.5 871,050 - EX31_RS21135 0.19 -1.4 871,050 - EX31_RS21135 0.19 -0.2 871,051 + EX31_RS21135 0.19 +0.8 871,051 + EX31_RS21135 0.19 +0.4 871,161 + EX31_RS21135 0.76 +0.5 871,161 + EX31_RS21135 0.76 -0.4 871,162 - EX31_RS21135 0.76 +0.4 871,162 - EX31_RS21135 0.76 +0.6 871,162 - EX31_RS21135 0.76 -0.1 871,218 + +0.7 871,218 + -1.9 871,218 + +2.2 871,218 + +0.2 871,219 - -0.0 871,219 - +0.4 871,238 + +0.6 871,238 + -1.0 871,238 + -1.1 871,239 - -0.5 871,239 - -0.6 871,239 - -0.5 871,239 - +0.1 871,239 - +0.5 871,239 - -0.1 871,406 + EX31_RS21140 0.14 -0.4 871,407 - EX31_RS21140 0.14 +0.4 871,407 - EX31_RS21140 0.14 +0.7 871,407 - EX31_RS21140 0.14 -0.6 871,407 - EX31_RS21140 0.14 +0.8 871,578 + EX31_RS21140 0.27 +0.1 871,578 + EX31_RS21140 0.27 +0.5 871,578 + EX31_RS21140 0.27 -0.5 871,578 + EX31_RS21140 0.27 -0.4 871,588 + EX31_RS21140 0.27 -0.1 871,621 + EX31_RS21140 0.30 -0.1 871,622 - EX31_RS21140 0.30 +0.2 871,732 - EX31_RS21140 0.38 +0.2 871,763 + EX31_RS21140 0.40 +0.6 871,763 + EX31_RS21140 0.40 +0.2 871,763 + EX31_RS21140 0.40 -0.8 871,763 + EX31_RS21140 0.40 +0.5 871,764 - EX31_RS21140 0.40 -0.1 871,764 - EX31_RS21140 0.40 +0.0 871,764 - EX31_RS21140 0.40 +0.1 871,764 - EX31_RS21140 0.40 +0.0 871,888 + EX31_RS21140 0.49 +1.2 871,888 + EX31_RS21140 0.49 -2.4 871,888 + EX31_RS21140 0.49 +1.3 871,889 - EX31_RS21140 0.49 -0.8 871,899 - EX31_RS21140 0.50 +1.2 871,899 - EX31_RS21140 0.50 -0.4 871,899 - EX31_RS21140 0.50 -0.2 871,965 + EX31_RS21140 0.54 +0.0 871,965 + EX31_RS21140 0.54 +0.3 872,009 - EX31_RS21140 0.57 +0.6 872,048 + EX31_RS21140 0.60 +0.8 872,048 + EX31_RS21140 0.60 +1.2 872,049 - EX31_RS21140 0.60 +0.2 872,128 - EX31_RS21140 0.66 -0.1 872,158 + EX31_RS21140 0.68 -0.3 872,158 + EX31_RS21140 0.68 -0.6 872,159 - EX31_RS21140 0.68 -0.2 872,248 + EX31_RS21140 0.74 -0.3 872,248 + EX31_RS21140 0.74 -1.3 872,248 + EX31_RS21140 0.74 -0.0 872,248 + EX31_RS21140 0.74 -0.8 872,248 + EX31_RS21140 0.74 -0.1 872,248 + EX31_RS21140 0.74 -0.2 872,248 + EX31_RS21140 0.74 +0.0 872,249 - EX31_RS21140 0.74 -1.2 872,249 - EX31_RS21140 0.74 -0.0 872,249 - EX31_RS21140 0.74 -1.3 872,249 - EX31_RS21140 0.74 -1.2 872,249 - EX31_RS21140 0.74 -0.5 872,249 - EX31_RS21140 0.74 +0.6 872,249 - EX31_RS21140 0.74 +1.5 872,409 + EX31_RS21140 0.86 -1.0 872,410 - EX31_RS21140 0.86 +0.5 872,498 + -0.6 872,498 + +1.2 872,498 + +0.7 872,498 + -0.0 872,498 + -0.7 872,498 + -0.5 872,498 + +0.9 872,499 - -0.6 872,499 - +0.7 872,499 - -0.6 872,499 - +0.7 872,499 - +0.6 872,499 - +1.4 872,499 - +1.4 872,662 - -0.3 872,662 - -0.9 872,676 + -0.1 872,677 - +0.9 872,719 + EX31_RS21145 0.15 -0.6 872,719 + EX31_RS21145 0.15 +0.3 872,720 - EX31_RS21145 0.15 -0.7 872,822 - EX31_RS21145 0.42 +0.5 872,873 + EX31_RS21145 0.55 -1.5 872,874 - EX31_RS21145 0.55 +0.6 872,898 + EX31_RS21145 0.61 -0.1 873,168 + +0.1 873,182 + -0.8 873,182 + +1.2 873,249 + +0.1 873,249 + +1.1 873,250 - +0.3 873,288 + +0.1 873,289 - -0.4 873,289 - +0.5 873,436 + +0.6 873,489 + -1.0 873,489 + +0.8 873,489 + -0.4 873,489 + -0.0 873,551 + -0.1 873,562 + +1.1 873,562 + -0.7 873,562 + +0.7 873,562 + +1.6 873,562 + -2.0 873,562 + +0.3 873,562 + +0.2 873,563 - -1.1 873,563 - +1.4 873,563 - +1.0 873,563 - -0.2 873,563 - +0.7 873,563 - +0.7 873,563 - +2.2 873,563 - +0.3 873,563 - +1.2 873,563 - +0.1 873,563 - +1.2 873,566 - +0.2 873,566 - +0.8 873,566 - -0.2 873,566 - +0.1 873,566 - -0.9 873,566 - +0.6 873,566 - +0.2 873,566 - +0.3 873,566 - -1.3 873,570 - +0.4
Or see this region's nucleotide sequence