Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17550 and EX31_RS17555 overlap by 4 nucleotides EX31_RS17555 and EX31_RS17560 are separated by 16 nucleotides EX31_RS17560 and EX31_RS17565 are separated by 573 nucleotides
EX31_RS17550: EX31_RS17550 - dipeptide/oligopeptide/nickel ABC transporter permease/ATP-binding protein, at 62,560 to 64,356
_RS17550
EX31_RS17555: EX31_RS17555 - ABC transporter ATP-binding protein, at 64,353 to 65,138
_RS17555
EX31_RS17560: EX31_RS17560 - FadR family transcriptional regulator, at 65,155 to 65,928
_RS17560
EX31_RS17565: EX31_RS17565 - GGDEF domain-containing protein, at 66,502 to 67,416
_RS17565
Position (kb) 65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 64.186 kb on - strand at 64.246 kb on + strand at 64.246 kb on + strand at 64.246 kb on + strand at 64.246 kb on + strand at 64.246 kb on + strand at 64.247 kb on - strand at 64.247 kb on - strand at 64.247 kb on - strand at 64.321 kb on + strand at 64.321 kb on + strand at 64.321 kb on + strand at 64.321 kb on + strand at 64.321 kb on + strand at 64.352 kb on + strand at 64.352 kb on + strand at 64.401 kb on - strand at 64.454 kb on - strand, within EX31_RS17555 at 64.512 kb on - strand, within EX31_RS17555 at 64.517 kb on + strand, within EX31_RS17555 at 64.602 kb on - strand, within EX31_RS17555 at 64.714 kb on - strand, within EX31_RS17555 at 64.714 kb on - strand, within EX31_RS17555 at 64.717 kb on + strand, within EX31_RS17555 at 64.717 kb on + strand, within EX31_RS17555 at 64.718 kb on - strand, within EX31_RS17555 at 64.742 kb on + strand, within EX31_RS17555 at 64.743 kb on - strand, within EX31_RS17555 at 64.743 kb on - strand, within EX31_RS17555 at 64.907 kb on - strand, within EX31_RS17555 at 64.907 kb on - strand, within EX31_RS17555 at 64.907 kb on - strand, within EX31_RS17555 at 64.907 kb on - strand, within EX31_RS17555 at 64.912 kb on + strand, within EX31_RS17555 at 64.912 kb on + strand, within EX31_RS17555 at 64.912 kb on + strand, within EX31_RS17555 at 64.913 kb on - strand, within EX31_RS17555 at 64.940 kb on - strand, within EX31_RS17555 at 64.974 kb on + strand, within EX31_RS17555 at 64.974 kb on + strand, within EX31_RS17555 at 64.975 kb on - strand, within EX31_RS17555 at 64.975 kb on - strand, within EX31_RS17555 at 64.983 kb on - strand, within EX31_RS17555 at 64.997 kb on - strand, within EX31_RS17555 at 65.037 kb on + strand, within EX31_RS17555 at 65.037 kb on + strand, within EX31_RS17555 at 65.037 kb on + strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.038 kb on - strand, within EX31_RS17555 at 65.050 kb on + strand, within EX31_RS17555 at 65.053 kb on + strand, within EX31_RS17555 at 65.053 kb on + strand, within EX31_RS17555 at 65.054 kb on - strand, within EX31_RS17555 at 65.100 kb on + strand at 65.136 kb on + strand at 65.154 kb on + strand at 65.394 kb on + strand, within EX31_RS17560 at 65.440 kb on + strand, within EX31_RS17560 at 65.472 kb on + strand, within EX31_RS17560 at 65.473 kb on - strand, within EX31_RS17560 at 65.473 kb on - strand, within EX31_RS17560 at 65.476 kb on + strand, within EX31_RS17560 at 65.476 kb on + strand, within EX31_RS17560 at 65.476 kb on + strand, within EX31_RS17560 at 65.476 kb on + strand, within EX31_RS17560 at 65.477 kb on - strand, within EX31_RS17560 at 65.477 kb on - strand, within EX31_RS17560 at 65.477 kb on - strand, within EX31_RS17560 at 65.656 kb on + strand, within EX31_RS17560 at 65.683 kb on - strand, within EX31_RS17560 at 65.733 kb on + strand, within EX31_RS17560 at 65.733 kb on + strand, within EX31_RS17560 at 65.737 kb on + strand, within EX31_RS17560 at 65.738 kb on - strand, within EX31_RS17560 at 65.738 kb on - strand, within EX31_RS17560 at 65.957 kb on + strand at 65.957 kb on + strand at 65.957 kb on + strand at 65.957 kb on + strand at 65.957 kb on + strand at 65.957 kb on + strand at 65.957 kb on + strand at 65.958 kb on - strand at 65.958 kb on - strand at 65.958 kb on - strand at 65.958 kb on - strand at 65.981 kb on + strand at 65.982 kb on - strand at 65.982 kb on - strand at 65.982 kb on - strand at 65.982 kb on - strand at 66.060 kb on + strand at 66.073 kb on + strand at 66.083 kb on + strand at 66.083 kb on + strand at 66.083 kb on + strand at 66.103 kb on + strand at 66.118 kb on + strand at 66.118 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.120 kb on + strand at 66.121 kb on - strand at 66.121 kb on - strand at 66.121 kb on - strand at 66.121 kb on - strand at 66.121 kb on - strand at 66.121 kb on - strand at 66.135 kb on - strand at 66.201 kb on + strand at 66.251 kb on - strand at 66.259 kb on - strand at 66.325 kb on + strand at 66.436 kb on + strand at 66.436 kb on + strand at 66.436 kb on + strand at 66.437 kb on - strand at 66.477 kb on + strand at 66.478 kb on - strand at 66.481 kb on + strand at 66.481 kb on + strand at 66.482 kb on - strand at 66.482 kb on - strand at 66.482 kb on - strand at 66.497 kb on + strand at 66.497 kb on + strand at 66.497 kb on + strand at 66.498 kb on - strand at 66.498 kb on - strand at 66.498 kb on - strand at 66.498 kb on - strand at 66.498 kb on - strand at 66.502 kb on - strand at 66.502 kb on - strand at 66.563 kb on - strand at 66.675 kb on - strand, within EX31_RS17565 at 66.698 kb on + strand, within EX31_RS17565 at 66.702 kb on + strand, within EX31_RS17565 at 66.703 kb on - strand, within EX31_RS17565 at 66.731 kb on + strand, within EX31_RS17565 at 66.847 kb on + strand, within EX31_RS17565 at 66.847 kb on + strand, within EX31_RS17565 at 66.848 kb on - strand, within EX31_RS17565 at 66.864 kb on + strand, within EX31_RS17565 at 66.876 kb on + strand, within EX31_RS17565 at 66.876 kb on + strand, within EX31_RS17565 at 66.876 kb on + strand, within EX31_RS17565 at 66.876 kb on + strand, within EX31_RS17565 at 66.877 kb on - strand, within EX31_RS17565 at 66.911 kb on + strand, within EX31_RS17565 at 66.916 kb on + strand, within EX31_RS17565 at 66.917 kb on - strand, within EX31_RS17565
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 64,186 - +0.3 64,246 + -0.2 64,246 + -0.5 64,246 + -2.6 64,246 + +0.8 64,246 + +1.2 64,247 - +1.2 64,247 - +1.3 64,247 - -0.8 64,321 + -0.8 64,321 + -0.0 64,321 + +0.9 64,321 + -0.4 64,321 + -0.7 64,352 + -0.0 64,352 + +0.6 64,401 - -0.3 64,454 - EX31_RS17555 0.13 -0.7 64,512 - EX31_RS17555 0.20 +1.5 64,517 + EX31_RS17555 0.21 +0.4 64,602 - EX31_RS17555 0.32 +0.6 64,714 - EX31_RS17555 0.46 +0.5 64,714 - EX31_RS17555 0.46 +0.8 64,717 + EX31_RS17555 0.46 +1.1 64,717 + EX31_RS17555 0.46 +0.4 64,718 - EX31_RS17555 0.46 -0.2 64,742 + EX31_RS17555 0.49 -0.2 64,743 - EX31_RS17555 0.50 -0.9 64,743 - EX31_RS17555 0.50 -0.6 64,907 - EX31_RS17555 0.70 -0.4 64,907 - EX31_RS17555 0.70 +2.4 64,907 - EX31_RS17555 0.70 -0.8 64,907 - EX31_RS17555 0.70 +0.8 64,912 + EX31_RS17555 0.71 +0.7 64,912 + EX31_RS17555 0.71 -0.8 64,912 + EX31_RS17555 0.71 +0.6 64,913 - EX31_RS17555 0.71 -2.0 64,940 - EX31_RS17555 0.75 +0.1 64,974 + EX31_RS17555 0.79 +1.2 64,974 + EX31_RS17555 0.79 +0.8 64,975 - EX31_RS17555 0.79 -1.5 64,975 - EX31_RS17555 0.79 -0.9 64,983 - EX31_RS17555 0.80 +1.0 64,997 - EX31_RS17555 0.82 -1.3 65,037 + EX31_RS17555 0.87 -0.2 65,037 + EX31_RS17555 0.87 +1.4 65,037 + EX31_RS17555 0.87 +0.2 65,038 - EX31_RS17555 0.87 +1.2 65,038 - EX31_RS17555 0.87 -0.3 65,038 - EX31_RS17555 0.87 -1.6 65,038 - EX31_RS17555 0.87 +0.3 65,038 - EX31_RS17555 0.87 +0.5 65,038 - EX31_RS17555 0.87 -0.9 65,050 + EX31_RS17555 0.89 +0.6 65,053 + EX31_RS17555 0.89 +0.7 65,053 + EX31_RS17555 0.89 -0.8 65,054 - EX31_RS17555 0.89 +0.4 65,100 + -0.1 65,136 + +0.1 65,154 + -0.0 65,394 + EX31_RS17560 0.31 +4.1 65,440 + EX31_RS17560 0.37 +0.5 65,472 + EX31_RS17560 0.41 -1.9 65,473 - EX31_RS17560 0.41 -1.5 65,473 - EX31_RS17560 0.41 +1.8 65,476 + EX31_RS17560 0.41 -1.0 65,476 + EX31_RS17560 0.41 +1.5 65,476 + EX31_RS17560 0.41 -0.2 65,476 + EX31_RS17560 0.41 +1.6 65,477 - EX31_RS17560 0.42 +1.6 65,477 - EX31_RS17560 0.42 +1.8 65,477 - EX31_RS17560 0.42 +0.2 65,656 + EX31_RS17560 0.65 +1.3 65,683 - EX31_RS17560 0.68 +1.2 65,733 + EX31_RS17560 0.75 -1.8 65,733 + EX31_RS17560 0.75 -0.9 65,737 + EX31_RS17560 0.75 -1.2 65,738 - EX31_RS17560 0.75 +0.8 65,738 - EX31_RS17560 0.75 +0.2 65,957 + +0.6 65,957 + -0.0 65,957 + +0.1 65,957 + +0.8 65,957 + -0.8 65,957 + -0.8 65,957 + -0.5 65,958 - +2.4 65,958 - -1.9 65,958 - +0.7 65,958 - +0.7 65,981 + +0.0 65,982 - +0.4 65,982 - -1.1 65,982 - -0.2 65,982 - +1.1 66,060 + -0.3 66,073 + +0.4 66,083 + -0.3 66,083 + +0.3 66,083 + -0.4 66,103 + +1.0 66,118 + +1.1 66,118 + -0.3 66,120 + -2.8 66,120 + +0.4 66,120 + +0.7 66,120 + +1.6 66,120 + -1.2 66,120 + +2.3 66,120 + +1.5 66,120 + -0.2 66,120 + +1.8 66,120 + +1.2 66,120 + +0.8 66,120 + -0.6 66,120 + +0.6 66,120 + -1.2 66,121 - +0.2 66,121 - -0.8 66,121 - -0.2 66,121 - +0.4 66,121 - +0.4 66,121 - -0.8 66,135 - +0.5 66,201 + -2.5 66,251 - +0.3 66,259 - +0.5 66,325 + +1.2 66,436 + -1.9 66,436 + -1.3 66,436 + +0.6 66,437 - -0.5 66,477 + +0.5 66,478 - +1.1 66,481 + -0.6 66,481 + -0.5 66,482 - -0.2 66,482 - +1.5 66,482 - +0.0 66,497 + +0.6 66,497 + -0.0 66,497 + -0.8 66,498 - +1.5 66,498 - +0.7 66,498 - +1.1 66,498 - -0.4 66,498 - +1.3 66,502 - +2.4 66,502 - -0.2 66,563 - +2.4 66,675 - EX31_RS17565 0.19 -2.2 66,698 + EX31_RS17565 0.21 -1.9 66,702 + EX31_RS17565 0.22 -0.5 66,703 - EX31_RS17565 0.22 +0.1 66,731 + EX31_RS17565 0.25 +1.6 66,847 + EX31_RS17565 0.38 -0.4 66,847 + EX31_RS17565 0.38 -0.6 66,848 - EX31_RS17565 0.38 +0.2 66,864 + EX31_RS17565 0.40 -1.5 66,876 + EX31_RS17565 0.41 -0.8 66,876 + EX31_RS17565 0.41 -0.8 66,876 + EX31_RS17565 0.41 +0.6 66,876 + EX31_RS17565 0.41 +0.5 66,877 - EX31_RS17565 0.41 +1.4 66,911 + EX31_RS17565 0.45 -0.8 66,916 + EX31_RS17565 0.45 -0.2 66,917 - EX31_RS17565 0.45 +1.8
Or see this region's nucleotide sequence