Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06715 and EX31_RS06720 overlap by 4 nucleotides EX31_RS06720 and EX31_RS06725 are separated by 170 nucleotides
EX31_RS06715: EX31_RS06715 - N-formylglutamate deformylase, at 127,625 to 128,461
_RS06715
EX31_RS06720: EX31_RS06720 - imidazolonepropionase, at 128,458 to 129,720
_RS06720
EX31_RS06725: EX31_RS06725 - histidine utilization repressor, at 129,891 to 130,709
_RS06725
Position (kb)
128
129
130 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 127.564 kb on - strand at 127.642 kb on + strand at 127.645 kb on - strand at 127.726 kb on - strand, within EX31_RS06715 at 127.752 kb on + strand, within EX31_RS06715 at 127.752 kb on + strand, within EX31_RS06715 at 127.752 kb on + strand, within EX31_RS06715 at 127.753 kb on - strand, within EX31_RS06715 at 127.753 kb on - strand, within EX31_RS06715 at 127.753 kb on - strand, within EX31_RS06715 at 127.753 kb on - strand, within EX31_RS06715 at 127.822 kb on + strand, within EX31_RS06715 at 127.823 kb on - strand, within EX31_RS06715 at 127.824 kb on + strand, within EX31_RS06715 at 127.824 kb on + strand, within EX31_RS06715 at 127.835 kb on - strand, within EX31_RS06715 at 127.846 kb on + strand, within EX31_RS06715 at 127.846 kb on + strand, within EX31_RS06715 at 128.154 kb on - strand, within EX31_RS06715 at 128.154 kb on - strand, within EX31_RS06715 at 128.167 kb on - strand, within EX31_RS06715 at 128.188 kb on + strand, within EX31_RS06715 at 128.190 kb on + strand, within EX31_RS06715 at 128.191 kb on - strand, within EX31_RS06715 at 128.387 kb on - strand at 128.407 kb on + strand at 128.408 kb on - strand at 128.480 kb on - strand at 128.480 kb on - strand at 128.563 kb on + strand at 128.564 kb on - strand at 128.682 kb on + strand, within EX31_RS06720 at 128.682 kb on + strand, within EX31_RS06720 at 128.682 kb on + strand, within EX31_RS06720 at 128.683 kb on - strand, within EX31_RS06720 at 128.683 kb on - strand, within EX31_RS06720 at 128.816 kb on + strand, within EX31_RS06720 at 128.833 kb on + strand, within EX31_RS06720 at 128.969 kb on - strand, within EX31_RS06720 at 129.003 kb on + strand, within EX31_RS06720 at 129.098 kb on - strand, within EX31_RS06720 at 129.120 kb on + strand, within EX31_RS06720 at 129.121 kb on - strand, within EX31_RS06720 at 129.121 kb on - strand, within EX31_RS06720 at 129.122 kb on + strand, within EX31_RS06720 at 129.122 kb on + strand, within EX31_RS06720 at 129.123 kb on - strand, within EX31_RS06720 at 129.123 kb on - strand, within EX31_RS06720 at 129.248 kb on - strand, within EX31_RS06720 at 129.271 kb on - strand, within EX31_RS06720 at 129.311 kb on - strand, within EX31_RS06720 at 129.311 kb on - strand, within EX31_RS06720 at 129.474 kb on + strand, within EX31_RS06720 at 129.621 kb on - strand at 129.661 kb on + strand at 129.661 kb on + strand at 129.661 kb on + strand at 129.662 kb on - strand at 129.662 kb on - strand at 129.759 kb on - strand at 129.769 kb on + strand at 129.820 kb on + strand at 129.876 kb on + strand at 129.919 kb on - strand at 129.919 kb on - strand at 129.941 kb on - strand at 130.108 kb on + strand, within EX31_RS06725 at 130.108 kb on + strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.109 kb on - strand, within EX31_RS06725 at 130.113 kb on - strand, within EX31_RS06725 at 130.113 kb on - strand, within EX31_RS06725 at 130.113 kb on - strand, within EX31_RS06725 at 130.113 kb on - strand, within EX31_RS06725 at 130.148 kb on - strand, within EX31_RS06725 at 130.211 kb on - strand, within EX31_RS06725 at 130.229 kb on - strand, within EX31_RS06725 at 130.229 kb on - strand, within EX31_RS06725 at 130.583 kb on - strand, within EX31_RS06725 at 130.598 kb on + strand, within EX31_RS06725 at 130.709 kb on + strand at 130.709 kb on + strand at 130.710 kb on - strand at 130.710 kb on - strand at 130.710 kb on - strand at 130.710 kb on - strand at 130.710 kb on - strand at 130.710 kb on - strand at 130.717 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 127,564 - -0.8 127,642 + +1.0 127,645 - +1.6 127,726 - EX31_RS06715 0.12 -1.0 127,752 + EX31_RS06715 0.15 +0.0 127,752 + EX31_RS06715 0.15 +3.4 127,752 + EX31_RS06715 0.15 +1.0 127,753 - EX31_RS06715 0.15 +0.5 127,753 - EX31_RS06715 0.15 -1.0 127,753 - EX31_RS06715 0.15 +0.8 127,753 - EX31_RS06715 0.15 -1.2 127,822 + EX31_RS06715 0.24 -0.5 127,823 - EX31_RS06715 0.24 -2.2 127,824 + EX31_RS06715 0.24 +0.0 127,824 + EX31_RS06715 0.24 +3.6 127,835 - EX31_RS06715 0.25 +0.4 127,846 + EX31_RS06715 0.26 +0.2 127,846 + EX31_RS06715 0.26 -0.8 128,154 - EX31_RS06715 0.63 +2.2 128,154 - EX31_RS06715 0.63 -1.4 128,167 - EX31_RS06715 0.65 +2.2 128,188 + EX31_RS06715 0.67 +2.1 128,190 + EX31_RS06715 0.68 +0.9 128,191 - EX31_RS06715 0.68 +1.5 128,387 - +1.8 128,407 + +1.1 128,408 - -0.8 128,480 - -1.0 128,480 - +1.1 128,563 + +0.3 128,564 - -1.0 128,682 + EX31_RS06720 0.18 +3.2 128,682 + EX31_RS06720 0.18 +0.6 128,682 + EX31_RS06720 0.18 +1.0 128,683 - EX31_RS06720 0.18 +0.0 128,683 - EX31_RS06720 0.18 +0.8 128,816 + EX31_RS06720 0.28 +0.0 128,833 + EX31_RS06720 0.30 -2.0 128,969 - EX31_RS06720 0.40 +1.6 129,003 + EX31_RS06720 0.43 +0.5 129,098 - EX31_RS06720 0.51 +0.9 129,120 + EX31_RS06720 0.52 +0.8 129,121 - EX31_RS06720 0.52 +1.6 129,121 - EX31_RS06720 0.52 +0.6 129,122 + EX31_RS06720 0.53 +1.0 129,122 + EX31_RS06720 0.53 -0.2 129,123 - EX31_RS06720 0.53 +0.4 129,123 - EX31_RS06720 0.53 -0.8 129,248 - EX31_RS06720 0.63 +0.6 129,271 - EX31_RS06720 0.64 +1.0 129,311 - EX31_RS06720 0.68 +1.4 129,311 - EX31_RS06720 0.68 +0.6 129,474 + EX31_RS06720 0.80 +0.2 129,621 - -1.2 129,661 + +0.8 129,661 + +2.0 129,661 + +0.0 129,662 - +0.6 129,662 - -0.3 129,759 - +0.0 129,769 + +0.9 129,820 + -0.2 129,876 + -0.1 129,919 - +1.7 129,919 - +1.0 129,941 - +0.2 130,108 + EX31_RS06725 0.26 +2.4 130,108 + EX31_RS06725 0.26 -0.7 130,109 - EX31_RS06725 0.27 +0.6 130,109 - EX31_RS06725 0.27 -0.2 130,109 - EX31_RS06725 0.27 +0.8 130,109 - EX31_RS06725 0.27 +0.0 130,109 - EX31_RS06725 0.27 -0.5 130,109 - EX31_RS06725 0.27 -0.1 130,109 - EX31_RS06725 0.27 +0.8 130,113 - EX31_RS06725 0.27 -1.2 130,113 - EX31_RS06725 0.27 -0.8 130,113 - EX31_RS06725 0.27 +1.0 130,113 - EX31_RS06725 0.27 -0.2 130,148 - EX31_RS06725 0.31 +0.0 130,211 - EX31_RS06725 0.39 +0.5 130,229 - EX31_RS06725 0.41 -0.6 130,229 - EX31_RS06725 0.41 +0.2 130,583 - EX31_RS06725 0.84 +0.4 130,598 + EX31_RS06725 0.86 -1.7 130,709 + -0.4 130,709 + -1.4 130,710 - +0.6 130,710 - +1.3 130,710 - -0.4 130,710 - +1.1 130,710 - -0.0 130,710 - +1.6 130,717 - +0.5
Or see this region's nucleotide sequence