Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS05730 and EX31_RS05735 are separated by 35 nucleotides EX31_RS05735 and EX31_RS05740 are separated by 6 nucleotides EX31_RS05740 and EX31_RS05745 are separated by 235 nucleotides
EX31_RS05730: EX31_RS05730 - isochorismatase family protein, at 297,106 to 297,660
_RS05730
EX31_RS05735: EX31_RS05735 - helix-turn-helix domain-containing protein, at 297,696 to 298,610
_RS05735
EX31_RS05740: EX31_RS05740 - acyltransferase, at 298,617 to 299,612
_RS05740
EX31_RS05745: EX31_RS05745 - MFS transporter, at 299,848 to 301,086
_RS05745
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1
2 at 297.664 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.695 kb on + strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.696 kb on - strand at 297.712 kb on - strand at 297.712 kb on - strand at 297.742 kb on + strand at 297.742 kb on + strand at 297.743 kb on - strand at 297.743 kb on - strand at 297.798 kb on + strand, within EX31_RS05735 at 297.798 kb on + strand, within EX31_RS05735 at 297.798 kb on + strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.799 kb on - strand, within EX31_RS05735 at 297.853 kb on + strand, within EX31_RS05735 at 297.854 kb on - strand, within EX31_RS05735 at 297.854 kb on - strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.009 kb on + strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.010 kb on - strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.013 kb on + strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.014 kb on - strand, within EX31_RS05735 at 298.141 kb on + strand, within EX31_RS05735 at 298.269 kb on + strand, within EX31_RS05735 at 298.269 kb on + strand, within EX31_RS05735 at 298.270 kb on - strand, within EX31_RS05735 at 298.270 kb on - strand, within EX31_RS05735 at 298.270 kb on - strand, within EX31_RS05735 at 298.270 kb on - strand, within EX31_RS05735 at 298.270 kb on - strand, within EX31_RS05735 at 298.322 kb on + strand, within EX31_RS05735 at 298.463 kb on + strand, within EX31_RS05735 at 298.479 kb on + strand, within EX31_RS05735 at 298.641 kb on + strand at 298.665 kb on + strand at 298.665 kb on + strand at 298.666 kb on - strand at 298.669 kb on - strand at 298.692 kb on - strand at 298.692 kb on - strand at 298.692 kb on - strand at 298.781 kb on - strand, within EX31_RS05740 at 298.781 kb on - strand, within EX31_RS05740 at 298.781 kb on - strand, within EX31_RS05740 at 298.878 kb on - strand, within EX31_RS05740 at 298.897 kb on + strand, within EX31_RS05740 at 298.898 kb on - strand, within EX31_RS05740 at 298.903 kb on + strand, within EX31_RS05740 at 298.903 kb on + strand, within EX31_RS05740 at 298.903 kb on + strand, within EX31_RS05740 at 298.903 kb on + strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.904 kb on - strand, within EX31_RS05740 at 298.928 kb on + strand, within EX31_RS05740 at 298.946 kb on - strand, within EX31_RS05740 at 298.949 kb on + strand, within EX31_RS05740 at 298.950 kb on - strand, within EX31_RS05740 at 298.950 kb on - strand, within EX31_RS05740 at 298.950 kb on - strand, within EX31_RS05740 at 299.007 kb on + strand, within EX31_RS05740 at 299.065 kb on + strand, within EX31_RS05740 at 299.068 kb on + strand, within EX31_RS05740 at 299.068 kb on + strand, within EX31_RS05740 at 299.069 kb on - strand, within EX31_RS05740 at 299.069 kb on - strand, within EX31_RS05740 at 299.089 kb on + strand, within EX31_RS05740 at 299.090 kb on - strand, within EX31_RS05740 at 299.091 kb on + strand, within EX31_RS05740 at 299.091 kb on + strand, within EX31_RS05740 at 299.091 kb on + strand, within EX31_RS05740 at 299.092 kb on - strand, within EX31_RS05740 at 299.092 kb on - strand, within EX31_RS05740 at 299.185 kb on + strand, within EX31_RS05740 at 299.185 kb on + strand, within EX31_RS05740 at 299.185 kb on + strand, within EX31_RS05740 at 299.186 kb on - strand, within EX31_RS05740 at 299.267 kb on - strand, within EX31_RS05740 at 299.334 kb on - strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.335 kb on + strand, within EX31_RS05740 at 299.336 kb on - strand, within EX31_RS05740 at 299.336 kb on - strand, within EX31_RS05740 at 299.336 kb on - strand, within EX31_RS05740 at 299.336 kb on - strand, within EX31_RS05740 at 299.336 kb on - strand, within EX31_RS05740 at 299.357 kb on - strand, within EX31_RS05740 at 299.370 kb on + strand, within EX31_RS05740 at 299.370 kb on + strand, within EX31_RS05740 at 299.370 kb on + strand, within EX31_RS05740 at 299.371 kb on - strand, within EX31_RS05740 at 299.371 kb on - strand, within EX31_RS05740 at 299.438 kb on + strand, within EX31_RS05740 at 299.586 kb on - strand at 299.590 kb on - strand at 299.631 kb on + strand at 299.632 kb on - strand at 299.632 kb on - strand at 299.671 kb on + strand at 299.739 kb on - strand at 299.739 kb on - strand at 299.760 kb on + strand at 299.760 kb on + strand at 299.797 kb on + strand at 299.840 kb on + strand at 299.840 kb on + strand at 299.845 kb on - strand at 299.853 kb on + strand at 299.854 kb on - strand at 299.866 kb on - strand at 299.935 kb on + strand at 299.935 kb on + strand at 299.935 kb on + strand at 299.935 kb on + strand at 299.935 kb on + strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.936 kb on - strand at 299.938 kb on - strand at 299.949 kb on + strand at 299.950 kb on - strand at 299.950 kb on - strand at 299.950 kb on - strand at 299.950 kb on - strand at 299.983 kb on - strand, within EX31_RS05745 at 300.028 kb on + strand, within EX31_RS05745 at 300.029 kb on - strand, within EX31_RS05745 at 300.029 kb on - strand, within EX31_RS05745 at 300.100 kb on - strand, within EX31_RS05745 at 300.153 kb on - strand, within EX31_RS05745 at 300.171 kb on + strand, within EX31_RS05745 at 300.171 kb on + strand, within EX31_RS05745 at 300.172 kb on - strand, within EX31_RS05745 at 300.172 kb on - strand, within EX31_RS05745 at 300.264 kb on + strand, within EX31_RS05745 at 300.265 kb on - strand, within EX31_RS05745 at 300.265 kb on - strand, within EX31_RS05745 at 300.265 kb on - strand, within EX31_RS05745 at 300.267 kb on + strand, within EX31_RS05745 at 300.268 kb on - strand, within EX31_RS05745 at 300.512 kb on + strand, within EX31_RS05745 at 300.585 kb on - strand, within EX31_RS05745 at 300.585 kb on - strand, within EX31_RS05745 at 300.602 kb on + strand, within EX31_RS05745 at 300.603 kb on - strand, within EX31_RS05745 at 300.610 kb on + strand, within EX31_RS05745 at 300.610 kb on + strand, within EX31_RS05745 at 300.611 kb on - strand, within EX31_RS05745 at 300.611 kb on - strand, within EX31_RS05745 at 300.611 kb on - strand, within EX31_RS05745
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 297,664 + -0.4 297,695 + +0.5 297,695 + -0.5 297,695 + -0.7 297,695 + +1.2 297,695 + -0.2 297,695 + +0.7 297,695 + -1.0 297,696 - -0.0 297,696 - -0.1 297,696 - -0.5 297,696 - +0.1 297,696 - +0.5 297,696 - -0.3 297,696 - -0.9 297,696 - +1.0 297,696 - -0.4 297,712 - +0.7 297,712 - -1.1 297,742 + -0.3 297,742 + -0.5 297,743 - -2.0 297,743 - +0.3 297,798 + EX31_RS05735 0.11 +0.3 297,798 + EX31_RS05735 0.11 -1.6 297,798 + EX31_RS05735 0.11 -0.6 297,799 - EX31_RS05735 0.11 -0.1 297,799 - EX31_RS05735 0.11 +0.7 297,799 - EX31_RS05735 0.11 -1.1 297,799 - EX31_RS05735 0.11 +0.0 297,799 - EX31_RS05735 0.11 -0.6 297,799 - EX31_RS05735 0.11 +0.3 297,799 - EX31_RS05735 0.11 +0.0 297,799 - EX31_RS05735 0.11 +0.5 297,799 - EX31_RS05735 0.11 +0.7 297,799 - EX31_RS05735 0.11 +1.1 297,799 - EX31_RS05735 0.11 -1.1 297,853 + EX31_RS05735 0.17 +0.6 297,854 - EX31_RS05735 0.17 -0.8 297,854 - EX31_RS05735 0.17 -0.4 298,009 + EX31_RS05735 0.34 +0.7 298,009 + EX31_RS05735 0.34 -0.4 298,009 + EX31_RS05735 0.34 +0.7 298,009 + EX31_RS05735 0.34 +0.7 298,009 + EX31_RS05735 0.34 +0.3 298,009 + EX31_RS05735 0.34 -0.5 298,009 + EX31_RS05735 0.34 +0.4 298,009 + EX31_RS05735 0.34 +0.9 298,009 + EX31_RS05735 0.34 -0.3 298,009 + EX31_RS05735 0.34 +1.0 298,009 + EX31_RS05735 0.34 +0.8 298,009 + EX31_RS05735 0.34 -1.9 298,009 + EX31_RS05735 0.34 -0.3 298,009 + EX31_RS05735 0.34 +0.8 298,009 + EX31_RS05735 0.34 +0.3 298,009 + EX31_RS05735 0.34 -1.9 298,010 - EX31_RS05735 0.34 -0.2 298,010 - EX31_RS05735 0.34 -1.5 298,010 - EX31_RS05735 0.34 -0.2 298,010 - EX31_RS05735 0.34 +0.5 298,010 - EX31_RS05735 0.34 -0.6 298,010 - EX31_RS05735 0.34 -0.4 298,010 - EX31_RS05735 0.34 -0.6 298,010 - EX31_RS05735 0.34 -0.0 298,010 - EX31_RS05735 0.34 -0.2 298,010 - EX31_RS05735 0.34 +0.4 298,010 - EX31_RS05735 0.34 -0.3 298,010 - EX31_RS05735 0.34 +1.6 298,010 - EX31_RS05735 0.34 -0.6 298,010 - EX31_RS05735 0.34 -0.4 298,010 - EX31_RS05735 0.34 -0.2 298,010 - EX31_RS05735 0.34 +0.7 298,010 - EX31_RS05735 0.34 +0.5 298,010 - EX31_RS05735 0.34 +0.0 298,013 + EX31_RS05735 0.35 +1.1 298,013 + EX31_RS05735 0.35 -0.7 298,013 + EX31_RS05735 0.35 -0.8 298,013 + EX31_RS05735 0.35 +0.3 298,013 + EX31_RS05735 0.35 +0.4 298,013 + EX31_RS05735 0.35 -0.2 298,013 + EX31_RS05735 0.35 +1.3 298,013 + EX31_RS05735 0.35 -1.0 298,014 - EX31_RS05735 0.35 +0.5 298,014 - EX31_RS05735 0.35 -1.3 298,014 - EX31_RS05735 0.35 -1.4 298,014 - EX31_RS05735 0.35 +2.2 298,014 - EX31_RS05735 0.35 +1.1 298,014 - EX31_RS05735 0.35 +1.0 298,014 - EX31_RS05735 0.35 +0.1 298,014 - EX31_RS05735 0.35 -0.4 298,141 + EX31_RS05735 0.49 +0.0 298,269 + EX31_RS05735 0.63 -0.1 298,269 + EX31_RS05735 0.63 -0.4 298,270 - EX31_RS05735 0.63 -0.9 298,270 - EX31_RS05735 0.63 -0.3 298,270 - EX31_RS05735 0.63 +0.5 298,270 - EX31_RS05735 0.63 -1.4 298,270 - EX31_RS05735 0.63 -1.6 298,322 + EX31_RS05735 0.68 -0.6 298,463 + EX31_RS05735 0.84 +1.1 298,479 + EX31_RS05735 0.86 +0.6 298,641 + +0.4 298,665 + +0.2 298,665 + +2.3 298,666 - +0.0 298,669 - -0.0 298,692 - +0.7 298,692 - +0.2 298,692 - -0.1 298,781 - EX31_RS05740 0.16 +0.6 298,781 - EX31_RS05740 0.16 +0.3 298,781 - EX31_RS05740 0.16 -0.2 298,878 - EX31_RS05740 0.26 -0.6 298,897 + EX31_RS05740 0.28 +0.3 298,898 - EX31_RS05740 0.28 -0.8 298,903 + EX31_RS05740 0.29 -0.2 298,903 + EX31_RS05740 0.29 -0.4 298,903 + EX31_RS05740 0.29 +1.0 298,903 + EX31_RS05740 0.29 -0.8 298,904 - EX31_RS05740 0.29 +0.3 298,904 - EX31_RS05740 0.29 +0.1 298,904 - EX31_RS05740 0.29 +0.8 298,904 - EX31_RS05740 0.29 +1.2 298,904 - EX31_RS05740 0.29 -0.3 298,904 - EX31_RS05740 0.29 +0.1 298,904 - EX31_RS05740 0.29 -0.3 298,928 + EX31_RS05740 0.31 +0.1 298,946 - EX31_RS05740 0.33 -0.7 298,949 + EX31_RS05740 0.33 -0.9 298,950 - EX31_RS05740 0.33 -0.1 298,950 - EX31_RS05740 0.33 -1.8 298,950 - EX31_RS05740 0.33 -0.9 299,007 + EX31_RS05740 0.39 -1.3 299,065 + EX31_RS05740 0.45 -1.2 299,068 + EX31_RS05740 0.45 -0.6 299,068 + EX31_RS05740 0.45 -0.2 299,069 - EX31_RS05740 0.45 +0.3 299,069 - EX31_RS05740 0.45 -0.7 299,089 + EX31_RS05740 0.47 +0.6 299,090 - EX31_RS05740 0.47 +0.1 299,091 + EX31_RS05740 0.48 +0.4 299,091 + EX31_RS05740 0.48 -0.0 299,091 + EX31_RS05740 0.48 -2.3 299,092 - EX31_RS05740 0.48 +0.2 299,092 - EX31_RS05740 0.48 +0.6 299,185 + EX31_RS05740 0.57 -0.2 299,185 + EX31_RS05740 0.57 -2.4 299,185 + EX31_RS05740 0.57 -2.2 299,186 - EX31_RS05740 0.57 -1.2 299,267 - EX31_RS05740 0.65 -1.0 299,334 - EX31_RS05740 0.72 +1.0 299,335 + EX31_RS05740 0.72 -0.6 299,335 + EX31_RS05740 0.72 -0.2 299,335 + EX31_RS05740 0.72 -0.9 299,335 + EX31_RS05740 0.72 +1.1 299,335 + EX31_RS05740 0.72 +0.0 299,335 + EX31_RS05740 0.72 -0.6 299,335 + EX31_RS05740 0.72 +0.5 299,336 - EX31_RS05740 0.72 -0.1 299,336 - EX31_RS05740 0.72 -0.7 299,336 - EX31_RS05740 0.72 +0.6 299,336 - EX31_RS05740 0.72 -2.4 299,336 - EX31_RS05740 0.72 -0.9 299,357 - EX31_RS05740 0.74 +0.5 299,370 + EX31_RS05740 0.76 +0.8 299,370 + EX31_RS05740 0.76 -0.9 299,370 + EX31_RS05740 0.76 -0.5 299,371 - EX31_RS05740 0.76 +0.4 299,371 - EX31_RS05740 0.76 -0.8 299,438 + EX31_RS05740 0.82 -1.2 299,586 - +0.5 299,590 - +0.4 299,631 + -0.8 299,632 - -0.3 299,632 - +0.4 299,671 + -0.3 299,739 - +0.1 299,739 - -0.2 299,760 + +0.1 299,760 + +0.7 299,797 + +0.3 299,840 + +0.0 299,840 + -2.6 299,845 - +0.1 299,853 + +1.0 299,854 - -1.7 299,866 - +0.0 299,935 + +1.3 299,935 + +0.4 299,935 + +1.1 299,935 + +1.3 299,935 + -0.7 299,936 - +0.5 299,936 - -0.4 299,936 - -1.1 299,936 - -1.6 299,936 - -0.3 299,936 - +0.6 299,936 - -1.2 299,936 - +0.0 299,936 - -0.5 299,936 - +0.5 299,938 - -0.3 299,949 + +1.2 299,950 - +0.1 299,950 - -0.9 299,950 - -0.4 299,950 - +0.0 299,983 - EX31_RS05745 0.11 +0.3 300,028 + EX31_RS05745 0.15 +0.3 300,029 - EX31_RS05745 0.15 +0.7 300,029 - EX31_RS05745 0.15 +0.6 300,100 - EX31_RS05745 0.20 +0.6 300,153 - EX31_RS05745 0.25 +0.3 300,171 + EX31_RS05745 0.26 +1.2 300,171 + EX31_RS05745 0.26 -0.1 300,172 - EX31_RS05745 0.26 +0.2 300,172 - EX31_RS05745 0.26 +0.5 300,264 + EX31_RS05745 0.34 -0.9 300,265 - EX31_RS05745 0.34 -1.6 300,265 - EX31_RS05745 0.34 +0.1 300,265 - EX31_RS05745 0.34 -0.2 300,267 + EX31_RS05745 0.34 -0.9 300,268 - EX31_RS05745 0.34 -0.4 300,512 + EX31_RS05745 0.54 -0.2 300,585 - EX31_RS05745 0.59 +0.1 300,585 - EX31_RS05745 0.59 +0.3 300,602 + EX31_RS05745 0.61 -0.3 300,603 - EX31_RS05745 0.61 +0.1 300,610 + EX31_RS05745 0.62 -0.1 300,610 + EX31_RS05745 0.62 +0.4 300,611 - EX31_RS05745 0.62 +1.1 300,611 - EX31_RS05745 0.62 -0.3 300,611 - EX31_RS05745 0.62 +0.3
Or see this region's nucleotide sequence