Experiment: R2A with nitrite 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20210 and CA265_RS20215 overlap by 29 nucleotides CA265_RS20215 and CA265_RS20220 are separated by 203 nucleotides CA265_RS20220 and CA265_RS20225 are separated by 32 nucleotides
CA265_RS20210: CA265_RS20210 - hypothetical protein, at 4,993,400 to 4,994,074
_RS20210
CA265_RS20215: CA265_RS20215 - transcriptional regulator, at 4,994,046 to 4,994,249
_RS20215
CA265_RS20220: CA265_RS20220 - hypothetical protein, at 4,994,453 to 4,995,529
_RS20220
CA265_RS20225: CA265_RS20225 - DNA (cytosine-5-)-methyltransferase, at 4,995,562 to 4,996,836
_RS20225
Position (kb)
4994
4995
4996 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4993.454 kb on + strand at 4993.455 kb on - strand at 4993.525 kb on + strand, within CA265_RS20210 at 4993.530 kb on - strand, within CA265_RS20210 at 4993.552 kb on + strand, within CA265_RS20210 at 4993.633 kb on + strand, within CA265_RS20210 at 4993.634 kb on - strand, within CA265_RS20210 at 4993.634 kb on - strand, within CA265_RS20210 at 4993.635 kb on + strand, within CA265_RS20210 at 4993.636 kb on - strand, within CA265_RS20210 at 4993.636 kb on - strand, within CA265_RS20210 at 4993.709 kb on - strand, within CA265_RS20210 at 4993.746 kb on - strand, within CA265_RS20210 at 4993.838 kb on + strand, within CA265_RS20210 at 4993.857 kb on - strand, within CA265_RS20210 at 4993.885 kb on - strand, within CA265_RS20210 at 4993.891 kb on + strand, within CA265_RS20210 at 4993.892 kb on - strand, within CA265_RS20210 at 4993.896 kb on - strand, within CA265_RS20210 at 4993.904 kb on - strand, within CA265_RS20210 at 4994.008 kb on + strand at 4994.112 kb on + strand, within CA265_RS20215 at 4994.118 kb on - strand, within CA265_RS20215 at 4994.146 kb on + strand, within CA265_RS20215 at 4994.166 kb on + strand, within CA265_RS20215 at 4994.198 kb on + strand, within CA265_RS20215 at 4994.217 kb on + strand, within CA265_RS20215 at 4994.218 kb on - strand, within CA265_RS20215 at 4994.290 kb on - strand at 4994.296 kb on + strand at 4994.305 kb on + strand at 4994.306 kb on - strand at 4994.306 kb on - strand at 4994.373 kb on - strand at 4994.524 kb on - strand at 4994.524 kb on - strand at 4994.618 kb on - strand, within CA265_RS20220 at 4994.638 kb on - strand, within CA265_RS20220 at 4994.811 kb on + strand, within CA265_RS20220 at 4994.822 kb on - strand, within CA265_RS20220 at 4994.829 kb on + strand, within CA265_RS20220 at 4994.835 kb on + strand, within CA265_RS20220 at 4994.835 kb on + strand, within CA265_RS20220 at 4994.835 kb on + strand, within CA265_RS20220 at 4994.835 kb on + strand, within CA265_RS20220 at 4994.836 kb on - strand, within CA265_RS20220 at 4994.887 kb on + strand, within CA265_RS20220 at 4994.905 kb on + strand, within CA265_RS20220 at 4994.949 kb on + strand, within CA265_RS20220 at 4995.028 kb on - strand, within CA265_RS20220 at 4995.037 kb on - strand, within CA265_RS20220 at 4995.039 kb on + strand, within CA265_RS20220 at 4995.062 kb on + strand at 4995.064 kb on + strand, within CA265_RS20220 at 4995.169 kb on - strand, within CA265_RS20220 at 4995.283 kb on - strand, within CA265_RS20220 at 4995.288 kb on + strand, within CA265_RS20220 at 4995.305 kb on - strand, within CA265_RS20220 at 4995.324 kb on - strand, within CA265_RS20220 at 4995.325 kb on + strand, within CA265_RS20220 at 4995.325 kb on + strand, within CA265_RS20220 at 4995.372 kb on + strand, within CA265_RS20220 at 4995.373 kb on - strand, within CA265_RS20220 at 4995.377 kb on + strand, within CA265_RS20220 at 4995.377 kb on + strand, within CA265_RS20220 at 4995.451 kb on + strand at 4995.452 kb on - strand at 4995.469 kb on + strand at 4995.469 kb on + strand at 4995.480 kb on + strand at 4995.491 kb on + strand at 4995.517 kb on - strand at 4995.517 kb on - strand at 4995.527 kb on + strand at 4995.527 kb on + strand at 4995.564 kb on - strand at 4995.568 kb on - strand at 4995.574 kb on - strand at 4995.774 kb on - strand, within CA265_RS20225 at 4995.794 kb on + strand, within CA265_RS20225 at 4996.017 kb on - strand, within CA265_RS20225 at 4996.523 kb on - strand, within CA265_RS20225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 3 mM remove 4,993,454 + +0.6 4,993,455 - +0.5 4,993,525 + CA265_RS20210 0.19 +0.6 4,993,530 - CA265_RS20210 0.19 -0.8 4,993,552 + CA265_RS20210 0.23 -1.9 4,993,633 + CA265_RS20210 0.35 +0.2 4,993,634 - CA265_RS20210 0.35 +0.3 4,993,634 - CA265_RS20210 0.35 +0.1 4,993,635 + CA265_RS20210 0.35 +0.3 4,993,636 - CA265_RS20210 0.35 +0.8 4,993,636 - CA265_RS20210 0.35 +0.7 4,993,709 - CA265_RS20210 0.46 -0.4 4,993,746 - CA265_RS20210 0.51 -0.0 4,993,838 + CA265_RS20210 0.65 -0.5 4,993,857 - CA265_RS20210 0.68 +0.0 4,993,885 - CA265_RS20210 0.72 -0.5 4,993,891 + CA265_RS20210 0.73 +1.2 4,993,892 - CA265_RS20210 0.73 +1.4 4,993,896 - CA265_RS20210 0.73 +0.1 4,993,904 - CA265_RS20210 0.75 +0.8 4,994,008 + -1.1 4,994,112 + CA265_RS20215 0.32 -0.8 4,994,118 - CA265_RS20215 0.35 +0.1 4,994,146 + CA265_RS20215 0.49 -0.2 4,994,166 + CA265_RS20215 0.59 +0.5 4,994,198 + CA265_RS20215 0.75 -0.3 4,994,217 + CA265_RS20215 0.84 -0.1 4,994,218 - CA265_RS20215 0.84 -0.9 4,994,290 - -1.4 4,994,296 + +0.0 4,994,305 + -0.2 4,994,306 - +0.1 4,994,306 - +1.5 4,994,373 - -0.1 4,994,524 - -2.5 4,994,524 - +0.2 4,994,618 - CA265_RS20220 0.15 +0.3 4,994,638 - CA265_RS20220 0.17 +0.4 4,994,811 + CA265_RS20220 0.33 +1.5 4,994,822 - CA265_RS20220 0.34 +0.5 4,994,829 + CA265_RS20220 0.35 +0.1 4,994,835 + CA265_RS20220 0.35 +0.3 4,994,835 + CA265_RS20220 0.35 +0.4 4,994,835 + CA265_RS20220 0.35 +0.5 4,994,835 + CA265_RS20220 0.35 +0.5 4,994,836 - CA265_RS20220 0.36 +0.9 4,994,887 + CA265_RS20220 0.40 -0.3 4,994,905 + CA265_RS20220 0.42 +0.3 4,994,949 + CA265_RS20220 0.46 -0.7 4,995,028 - CA265_RS20220 0.53 -0.6 4,995,037 - CA265_RS20220 0.54 -0.2 4,995,039 + CA265_RS20220 0.54 +1.0 4,995,062 + +2.8 4,995,064 + CA265_RS20220 0.57 -0.3 4,995,169 - CA265_RS20220 0.66 -0.8 4,995,283 - CA265_RS20220 0.77 +1.0 4,995,288 + CA265_RS20220 0.78 -0.8 4,995,305 - CA265_RS20220 0.79 +0.3 4,995,324 - CA265_RS20220 0.81 +0.7 4,995,325 + CA265_RS20220 0.81 +0.4 4,995,325 + CA265_RS20220 0.81 -0.5 4,995,372 + CA265_RS20220 0.85 +1.3 4,995,373 - CA265_RS20220 0.85 +0.9 4,995,377 + CA265_RS20220 0.86 +0.6 4,995,377 + CA265_RS20220 0.86 +0.9 4,995,451 + +1.0 4,995,452 - +0.4 4,995,469 + -0.3 4,995,469 + +0.3 4,995,480 + +0.4 4,995,491 + -0.5 4,995,517 - +2.4 4,995,517 - +0.2 4,995,527 + +0.1 4,995,527 + -1.0 4,995,564 - +0.1 4,995,568 - +0.6 4,995,574 - -0.1 4,995,774 - CA265_RS20225 0.17 +0.8 4,995,794 + CA265_RS20225 0.18 -2.4 4,996,017 - CA265_RS20225 0.36 +0.0 4,996,523 - CA265_RS20225 0.75 -1.4
Or see this region's nucleotide sequence