Experiment: R2A with nitrite 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07940 and CA265_RS07945 overlap by 4 nucleotides CA265_RS07945 and CA265_RS07950 are separated by 6 nucleotides
CA265_RS07940: CA265_RS07940 - capsular biosynthesis protein, at 1,981,136 to 1,982,410
_RS07940
CA265_RS07945: CA265_RS07945 - glycosyl transferase, at 1,982,407 to 1,983,009
_RS07945
CA265_RS07950: CA265_RS07950 - hypothetical protein, at 1,983,016 to 1,984,116
_RS07950
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-1
0
1
2 at 1981.424 kb on - strand, within CA265_RS07940 at 1981.437 kb on + strand, within CA265_RS07940 at 1981.438 kb on - strand, within CA265_RS07940 at 1981.438 kb on - strand, within CA265_RS07940 at 1981.538 kb on - strand, within CA265_RS07940 at 1981.663 kb on - strand at 1981.704 kb on + strand, within CA265_RS07940 at 1981.761 kb on + strand, within CA265_RS07940 at 1981.814 kb on + strand, within CA265_RS07940 at 1981.835 kb on - strand, within CA265_RS07940 at 1981.880 kb on - strand, within CA265_RS07940 at 1981.915 kb on + strand, within CA265_RS07940 at 1981.915 kb on + strand, within CA265_RS07940 at 1981.917 kb on + strand, within CA265_RS07940 at 1981.917 kb on + strand, within CA265_RS07940 at 1981.952 kb on + strand, within CA265_RS07940 at 1982.069 kb on + strand, within CA265_RS07940 at 1982.070 kb on - strand, within CA265_RS07940 at 1982.158 kb on + strand, within CA265_RS07940 at 1982.158 kb on + strand, within CA265_RS07940 at 1982.158 kb on + strand, within CA265_RS07940 at 1982.159 kb on - strand, within CA265_RS07940 at 1982.163 kb on + strand, within CA265_RS07940 at 1982.213 kb on - strand, within CA265_RS07940 at 1982.246 kb on + strand, within CA265_RS07940 at 1982.274 kb on - strand, within CA265_RS07940 at 1982.335 kb on + strand at 1982.336 kb on - strand at 1982.337 kb on + strand at 1982.378 kb on - strand at 1982.400 kb on + strand at 1982.400 kb on + strand at 1982.556 kb on + strand, within CA265_RS07945 at 1982.577 kb on - strand, within CA265_RS07945 at 1982.703 kb on - strand, within CA265_RS07945 at 1982.742 kb on + strand, within CA265_RS07945 at 1982.765 kb on + strand, within CA265_RS07945 at 1982.773 kb on + strand, within CA265_RS07945 at 1982.815 kb on - strand, within CA265_RS07945 at 1982.817 kb on + strand, within CA265_RS07945 at 1982.818 kb on - strand, within CA265_RS07945 at 1982.890 kb on + strand, within CA265_RS07945 at 1982.905 kb on - strand, within CA265_RS07945 at 1982.919 kb on - strand, within CA265_RS07945 at 1982.936 kb on - strand, within CA265_RS07945 at 1982.936 kb on - strand, within CA265_RS07945 at 1982.953 kb on - strand at 1983.020 kb on - strand at 1983.020 kb on - strand at 1983.139 kb on - strand, within CA265_RS07950 at 1983.269 kb on - strand, within CA265_RS07950 at 1983.296 kb on - strand, within CA265_RS07950 at 1983.296 kb on - strand, within CA265_RS07950 at 1983.324 kb on + strand, within CA265_RS07950 at 1983.328 kb on - strand, within CA265_RS07950 at 1983.328 kb on - strand, within CA265_RS07950 at 1983.523 kb on + strand, within CA265_RS07950 at 1983.523 kb on + strand, within CA265_RS07950 at 1983.524 kb on - strand, within CA265_RS07950 at 1983.524 kb on - strand, within CA265_RS07950 at 1983.525 kb on + strand, within CA265_RS07950 at 1983.552 kb on - strand, within CA265_RS07950 at 1983.624 kb on - strand, within CA265_RS07950 at 1983.815 kb on + strand, within CA265_RS07950 at 1983.833 kb on + strand, within CA265_RS07950 at 1983.878 kb on + strand, within CA265_RS07950 at 1983.889 kb on - strand, within CA265_RS07950 at 1983.889 kb on - strand, within CA265_RS07950 at 1983.889 kb on - strand, within CA265_RS07950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 3 mM remove 1,981,424 - CA265_RS07940 0.23 -0.1 1,981,437 + CA265_RS07940 0.24 -1.0 1,981,438 - CA265_RS07940 0.24 -0.1 1,981,438 - CA265_RS07940 0.24 +0.2 1,981,538 - CA265_RS07940 0.32 -0.3 1,981,663 - +0.2 1,981,704 + CA265_RS07940 0.45 +0.5 1,981,761 + CA265_RS07940 0.49 +1.0 1,981,814 + CA265_RS07940 0.53 +0.1 1,981,835 - CA265_RS07940 0.55 +0.3 1,981,880 - CA265_RS07940 0.58 -0.1 1,981,915 + CA265_RS07940 0.61 +0.1 1,981,915 + CA265_RS07940 0.61 +0.2 1,981,917 + CA265_RS07940 0.61 +0.2 1,981,917 + CA265_RS07940 0.61 +0.2 1,981,952 + CA265_RS07940 0.64 +0.9 1,982,069 + CA265_RS07940 0.73 -0.5 1,982,070 - CA265_RS07940 0.73 -0.4 1,982,158 + CA265_RS07940 0.80 +0.2 1,982,158 + CA265_RS07940 0.80 -0.8 1,982,158 + CA265_RS07940 0.80 +0.3 1,982,159 - CA265_RS07940 0.80 -0.2 1,982,163 + CA265_RS07940 0.81 -0.0 1,982,213 - CA265_RS07940 0.84 +0.2 1,982,246 + CA265_RS07940 0.87 +0.8 1,982,274 - CA265_RS07940 0.89 +0.2 1,982,335 + -0.4 1,982,336 - +0.3 1,982,337 + +0.1 1,982,378 - +0.9 1,982,400 + -0.3 1,982,400 + +0.1 1,982,556 + CA265_RS07945 0.25 -0.7 1,982,577 - CA265_RS07945 0.28 -0.4 1,982,703 - CA265_RS07945 0.49 -0.5 1,982,742 + CA265_RS07945 0.56 +0.5 1,982,765 + CA265_RS07945 0.59 -0.3 1,982,773 + CA265_RS07945 0.61 -0.2 1,982,815 - CA265_RS07945 0.68 +0.0 1,982,817 + CA265_RS07945 0.68 +1.7 1,982,818 - CA265_RS07945 0.68 +1.2 1,982,890 + CA265_RS07945 0.80 +1.9 1,982,905 - CA265_RS07945 0.83 +0.2 1,982,919 - CA265_RS07945 0.85 -0.3 1,982,936 - CA265_RS07945 0.88 -0.2 1,982,936 - CA265_RS07945 0.88 -0.0 1,982,953 - -0.0 1,983,020 - +0.2 1,983,020 - +0.1 1,983,139 - CA265_RS07950 0.11 +0.6 1,983,269 - CA265_RS07950 0.23 +0.8 1,983,296 - CA265_RS07950 0.25 -0.3 1,983,296 - CA265_RS07950 0.25 -0.1 1,983,324 + CA265_RS07950 0.28 +0.2 1,983,328 - CA265_RS07950 0.28 -1.5 1,983,328 - CA265_RS07950 0.28 +1.2 1,983,523 + CA265_RS07950 0.46 -0.0 1,983,523 + CA265_RS07950 0.46 -0.9 1,983,524 - CA265_RS07950 0.46 -0.6 1,983,524 - CA265_RS07950 0.46 -0.8 1,983,525 + CA265_RS07950 0.46 -0.2 1,983,552 - CA265_RS07950 0.49 -0.2 1,983,624 - CA265_RS07950 0.55 -1.3 1,983,815 + CA265_RS07950 0.73 -0.4 1,983,833 + CA265_RS07950 0.74 +0.0 1,983,878 + CA265_RS07950 0.78 +0.1 1,983,889 - CA265_RS07950 0.79 -1.4 1,983,889 - CA265_RS07950 0.79 +0.2 1,983,889 - CA265_RS07950 0.79 -1.4
Or see this region's nucleotide sequence