Experiment: R2A with nitrite 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00385 and CA265_RS00390 are separated by 30 nucleotides CA265_RS00390 and CA265_RS00395 are separated by 53 nucleotides CA265_RS00395 and CA265_RS00400 are separated by 45 nucleotides
CA265_RS00385: CA265_RS00385 - hypothetical protein, at 86,659 to 87,456
_RS00385
CA265_RS00390: CA265_RS00390 - hypothetical protein, at 87,487 to 87,993
_RS00390
CA265_RS00395: CA265_RS00395 - hypothetical protein, at 88,047 to 88,325
_RS00395
CA265_RS00400: CA265_RS00400 - hypothetical protein, at 88,371 to 90,113
_RS00400
Position (kb)
87
88 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 86.523 kb on + strand at 86.523 kb on + strand at 86.524 kb on - strand at 86.524 kb on - strand at 86.524 kb on - strand at 86.654 kb on + strand at 86.654 kb on + strand at 86.659 kb on - strand at 86.854 kb on + strand, within CA265_RS00385 at 86.858 kb on + strand, within CA265_RS00385 at 86.858 kb on + strand, within CA265_RS00385 at 86.880 kb on - strand, within CA265_RS00385 at 86.914 kb on - strand, within CA265_RS00385 at 86.923 kb on - strand, within CA265_RS00385 at 86.979 kb on - strand, within CA265_RS00385 at 86.979 kb on - strand, within CA265_RS00385 at 86.997 kb on + strand, within CA265_RS00385 at 87.166 kb on + strand, within CA265_RS00385 at 87.173 kb on + strand, within CA265_RS00385 at 87.279 kb on + strand, within CA265_RS00385 at 87.280 kb on - strand, within CA265_RS00385 at 87.280 kb on - strand, within CA265_RS00385 at 87.306 kb on - strand, within CA265_RS00385 at 87.378 kb on + strand at 87.379 kb on - strand at 87.447 kb on - strand at 87.509 kb on + strand at 87.557 kb on + strand, within CA265_RS00390 at 87.613 kb on + strand, within CA265_RS00390 at 87.614 kb on - strand, within CA265_RS00390 at 87.618 kb on - strand, within CA265_RS00390 at 87.626 kb on - strand, within CA265_RS00390 at 87.629 kb on - strand, within CA265_RS00390 at 87.652 kb on + strand, within CA265_RS00390 at 87.652 kb on + strand, within CA265_RS00390 at 87.655 kb on + strand, within CA265_RS00390 at 87.655 kb on + strand, within CA265_RS00390 at 87.656 kb on - strand, within CA265_RS00390 at 87.676 kb on - strand, within CA265_RS00390 at 87.687 kb on + strand, within CA265_RS00390 at 87.729 kb on + strand, within CA265_RS00390 at 87.730 kb on - strand, within CA265_RS00390 at 87.748 kb on - strand, within CA265_RS00390 at 87.756 kb on + strand, within CA265_RS00390 at 87.769 kb on - strand, within CA265_RS00390 at 87.809 kb on + strand, within CA265_RS00390 at 87.852 kb on - strand, within CA265_RS00390 at 87.852 kb on - strand, within CA265_RS00390 at 87.892 kb on - strand, within CA265_RS00390 at 87.916 kb on - strand, within CA265_RS00390 at 87.940 kb on - strand, within CA265_RS00390 at 88.003 kb on + strand at 88.048 kb on + strand at 88.158 kb on - strand, within CA265_RS00395 at 88.173 kb on - strand, within CA265_RS00395 at 88.199 kb on + strand, within CA265_RS00395 at 88.227 kb on + strand, within CA265_RS00395 at 88.317 kb on + strand at 88.317 kb on + strand at 88.318 kb on - strand at 88.318 kb on - strand at 88.341 kb on - strand at 88.355 kb on - strand at 88.380 kb on - strand at 88.387 kb on + strand at 88.388 kb on - strand at 88.467 kb on - strand at 88.469 kb on - strand at 88.521 kb on + strand at 88.553 kb on + strand, within CA265_RS00400 at 88.553 kb on + strand, within CA265_RS00400 at 88.561 kb on + strand, within CA265_RS00400 at 88.660 kb on + strand, within CA265_RS00400 at 88.662 kb on + strand, within CA265_RS00400 at 88.663 kb on - strand, within CA265_RS00400 at 88.680 kb on - strand, within CA265_RS00400 at 88.680 kb on - strand, within CA265_RS00400 at 88.680 kb on - strand, within CA265_RS00400 at 88.683 kb on + strand, within CA265_RS00400 at 88.738 kb on + strand, within CA265_RS00400 at 88.770 kb on + strand, within CA265_RS00400 at 88.807 kb on + strand, within CA265_RS00400 at 88.811 kb on - strand, within CA265_RS00400 at 88.842 kb on - strand, within CA265_RS00400 at 88.896 kb on + strand, within CA265_RS00400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 3 mM remove 86,523 + +0.4 86,523 + +0.1 86,524 - +0.1 86,524 - -1.0 86,524 - -1.6 86,654 + +0.7 86,654 + -0.3 86,659 - -0.3 86,854 + CA265_RS00385 0.24 -2.3 86,858 + CA265_RS00385 0.25 -0.5 86,858 + CA265_RS00385 0.25 +0.2 86,880 - CA265_RS00385 0.28 +0.4 86,914 - CA265_RS00385 0.32 +0.1 86,923 - CA265_RS00385 0.33 +0.2 86,979 - CA265_RS00385 0.40 -0.4 86,979 - CA265_RS00385 0.40 +0.7 86,997 + CA265_RS00385 0.42 +0.8 87,166 + CA265_RS00385 0.64 -0.5 87,173 + CA265_RS00385 0.64 +0.8 87,279 + CA265_RS00385 0.78 -0.4 87,280 - CA265_RS00385 0.78 -2.2 87,280 - CA265_RS00385 0.78 +0.8 87,306 - CA265_RS00385 0.81 +0.9 87,378 + +0.2 87,379 - +0.1 87,447 - +0.2 87,509 + -0.3 87,557 + CA265_RS00390 0.14 -0.5 87,613 + CA265_RS00390 0.25 +0.1 87,614 - CA265_RS00390 0.25 -0.2 87,618 - CA265_RS00390 0.26 +0.1 87,626 - CA265_RS00390 0.27 +0.4 87,629 - CA265_RS00390 0.28 -0.7 87,652 + CA265_RS00390 0.33 +1.1 87,652 + CA265_RS00390 0.33 -0.0 87,655 + CA265_RS00390 0.33 -0.0 87,655 + CA265_RS00390 0.33 -0.0 87,656 - CA265_RS00390 0.33 +0.5 87,676 - CA265_RS00390 0.37 -3.1 87,687 + CA265_RS00390 0.39 +0.1 87,729 + CA265_RS00390 0.48 -0.5 87,730 - CA265_RS00390 0.48 -0.9 87,748 - CA265_RS00390 0.51 -0.3 87,756 + CA265_RS00390 0.53 -0.1 87,769 - CA265_RS00390 0.56 +0.2 87,809 + CA265_RS00390 0.64 -0.8 87,852 - CA265_RS00390 0.72 -0.1 87,852 - CA265_RS00390 0.72 -2.0 87,892 - CA265_RS00390 0.80 +0.3 87,916 - CA265_RS00390 0.85 +0.2 87,940 - CA265_RS00390 0.89 -1.0 88,003 + -0.9 88,048 + -1.0 88,158 - CA265_RS00395 0.40 -0.8 88,173 - CA265_RS00395 0.45 +1.1 88,199 + CA265_RS00395 0.54 +0.6 88,227 + CA265_RS00395 0.65 +0.2 88,317 + -0.3 88,317 + +0.2 88,318 - +0.7 88,318 - -0.7 88,341 - -1.3 88,355 - -1.0 88,380 - -1.2 88,387 + -0.4 88,388 - -0.3 88,467 - -0.4 88,469 - +0.2 88,521 + +0.2 88,553 + CA265_RS00400 0.10 +0.8 88,553 + CA265_RS00400 0.10 -0.5 88,561 + CA265_RS00400 0.11 +0.5 88,660 + CA265_RS00400 0.17 +0.2 88,662 + CA265_RS00400 0.17 +0.4 88,663 - CA265_RS00400 0.17 -1.0 88,680 - CA265_RS00400 0.18 -0.3 88,680 - CA265_RS00400 0.18 +0.0 88,680 - CA265_RS00400 0.18 +0.0 88,683 + CA265_RS00400 0.18 +0.1 88,738 + CA265_RS00400 0.21 -0.8 88,770 + CA265_RS00400 0.23 +0.2 88,807 + CA265_RS00400 0.25 -0.2 88,811 - CA265_RS00400 0.25 -0.0 88,842 - CA265_RS00400 0.27 -1.2 88,896 + CA265_RS00400 0.30 +0.3
Or see this region's nucleotide sequence