Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1117 and Ac3H11_1118 are separated by 85 nucleotides Ac3H11_1118 and Ac3H11_1119 are separated by 138 nucleotides Ac3H11_1119 and Ac3H11_1120 are separated by 45 nucleotides
Ac3H11_1117 - Guanine deaminase (EC 3.5.4.3), at 59,818 to 61,092
_1117
Ac3H11_1118 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 61,178 to 61,750
_1118
Ac3H11_1119 - hypothetical protein, at 61,889 to 62,170
_1119
Ac3H11_1120 - Diguanylate cyclase/phosphodiesterase domain 1 (GGDEF), at 62,216 to 63,916
_1120
Position (kb)
61
62 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 60.370 kb on + strand, within Ac3H11_1117 at 60.532 kb on + strand, within Ac3H11_1117 at 60.532 kb on + strand, within Ac3H11_1117 at 60.532 kb on + strand, within Ac3H11_1117 at 60.532 kb on + strand, within Ac3H11_1117 at 60.532 kb on + strand, within Ac3H11_1117 at 60.533 kb on - strand, within Ac3H11_1117 at 60.533 kb on - strand, within Ac3H11_1117 at 60.533 kb on - strand, within Ac3H11_1117 at 60.533 kb on - strand, within Ac3H11_1117 at 60.533 kb on - strand, within Ac3H11_1117 at 60.544 kb on + strand, within Ac3H11_1117 at 60.544 kb on + strand, within Ac3H11_1117 at 60.545 kb on - strand, within Ac3H11_1117 at 60.545 kb on - strand, within Ac3H11_1117 at 60.580 kb on + strand, within Ac3H11_1117 at 60.581 kb on - strand, within Ac3H11_1117 at 60.581 kb on - strand, within Ac3H11_1117 at 60.581 kb on - strand, within Ac3H11_1117 at 60.694 kb on + strand, within Ac3H11_1117 at 60.694 kb on + strand, within Ac3H11_1117 at 60.694 kb on + strand, within Ac3H11_1117 at 60.695 kb on - strand, within Ac3H11_1117 at 60.695 kb on - strand, within Ac3H11_1117 at 60.695 kb on - strand, within Ac3H11_1117 at 60.695 kb on - strand, within Ac3H11_1117 at 60.790 kb on + strand, within Ac3H11_1117 at 60.790 kb on + strand, within Ac3H11_1117 at 60.790 kb on + strand, within Ac3H11_1117 at 60.790 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.793 kb on + strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.794 kb on - strand, within Ac3H11_1117 at 60.848 kb on + strand, within Ac3H11_1117 at 60.848 kb on + strand, within Ac3H11_1117 at 60.848 kb on + strand, within Ac3H11_1117 at 60.848 kb on + strand, within Ac3H11_1117 at 60.849 kb on - strand, within Ac3H11_1117 at 60.849 kb on - strand, within Ac3H11_1117 at 60.849 kb on - strand, within Ac3H11_1117 at 61.177 kb on + strand at 61.177 kb on + strand at 61.177 kb on + strand at 61.177 kb on + strand at 61.790 kb on + strand at 61.790 kb on + strand at 61.791 kb on - strand at 61.791 kb on - strand at 61.838 kb on + strand at 61.839 kb on - strand at 61.918 kb on + strand, within Ac3H11_1119 at 61.918 kb on + strand, within Ac3H11_1119 at 61.919 kb on - strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.973 kb on + strand, within Ac3H11_1119 at 61.973 kb on + strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 62.067 kb on - strand, within Ac3H11_1119 at 62.144 kb on + strand at 62.145 kb on - strand at 62.145 kb on - strand at 62.145 kb on - strand at 62.263 kb on - strand at 62.550 kb on + strand, within Ac3H11_1120 at 62.551 kb on - strand, within Ac3H11_1120 at 62.551 kb on - strand, within Ac3H11_1120
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 60,370 + Ac3H11_1117 0.43 -3.0 60,532 + Ac3H11_1117 0.56 +0.0 60,532 + Ac3H11_1117 0.56 -0.2 60,532 + Ac3H11_1117 0.56 -0.3 60,532 + Ac3H11_1117 0.56 -0.7 60,532 + Ac3H11_1117 0.56 +0.3 60,533 - Ac3H11_1117 0.56 -0.4 60,533 - Ac3H11_1117 0.56 +0.7 60,533 - Ac3H11_1117 0.56 +1.4 60,533 - Ac3H11_1117 0.56 -0.2 60,533 - Ac3H11_1117 0.56 -0.3 60,544 + Ac3H11_1117 0.57 +0.1 60,544 + Ac3H11_1117 0.57 +1.7 60,545 - Ac3H11_1117 0.57 -0.5 60,545 - Ac3H11_1117 0.57 +0.6 60,580 + Ac3H11_1117 0.60 -0.1 60,581 - Ac3H11_1117 0.60 -1.1 60,581 - Ac3H11_1117 0.60 +0.6 60,581 - Ac3H11_1117 0.60 +1.0 60,694 + Ac3H11_1117 0.69 +0.8 60,694 + Ac3H11_1117 0.69 +0.5 60,694 + Ac3H11_1117 0.69 -1.2 60,695 - Ac3H11_1117 0.69 -1.2 60,695 - Ac3H11_1117 0.69 +0.3 60,695 - Ac3H11_1117 0.69 +0.8 60,695 - Ac3H11_1117 0.69 -0.1 60,790 + Ac3H11_1117 0.76 -1.4 60,790 + Ac3H11_1117 0.76 -0.4 60,790 + Ac3H11_1117 0.76 +0.4 60,790 + Ac3H11_1117 0.76 -2.7 60,793 + Ac3H11_1117 0.76 -0.2 60,793 + Ac3H11_1117 0.76 +0.2 60,793 + Ac3H11_1117 0.76 +0.7 60,793 + Ac3H11_1117 0.76 +0.0 60,793 + Ac3H11_1117 0.76 +0.1 60,793 + Ac3H11_1117 0.76 -0.2 60,793 + Ac3H11_1117 0.76 -0.4 60,793 + Ac3H11_1117 0.76 +1.3 60,794 - Ac3H11_1117 0.77 +0.5 60,794 - Ac3H11_1117 0.77 +0.9 60,794 - Ac3H11_1117 0.77 +0.2 60,794 - Ac3H11_1117 0.77 +0.8 60,794 - Ac3H11_1117 0.77 -0.3 60,794 - Ac3H11_1117 0.77 -1.2 60,794 - Ac3H11_1117 0.77 +0.5 60,794 - Ac3H11_1117 0.77 -0.4 60,794 - Ac3H11_1117 0.77 +0.0 60,794 - Ac3H11_1117 0.77 +0.1 60,794 - Ac3H11_1117 0.77 +0.1 60,794 - Ac3H11_1117 0.77 +0.2 60,848 + Ac3H11_1117 0.81 +1.5 60,848 + Ac3H11_1117 0.81 +0.9 60,848 + Ac3H11_1117 0.81 -0.5 60,848 + Ac3H11_1117 0.81 -0.2 60,849 - Ac3H11_1117 0.81 +0.0 60,849 - Ac3H11_1117 0.81 -0.7 60,849 - Ac3H11_1117 0.81 +0.6 61,177 + -1.9 61,177 + -1.2 61,177 + -0.7 61,177 + -0.5 61,790 + -0.9 61,790 + +0.3 61,791 - +0.1 61,791 - +0.4 61,838 + -1.1 61,839 - -0.5 61,918 + Ac3H11_1119 0.10 -1.2 61,918 + Ac3H11_1119 0.10 -2.2 61,919 - Ac3H11_1119 0.11 -1.1 61,921 + Ac3H11_1119 0.11 -0.5 61,921 + Ac3H11_1119 0.11 +0.8 61,921 + Ac3H11_1119 0.11 +1.1 61,922 - Ac3H11_1119 0.12 +0.5 61,922 - Ac3H11_1119 0.12 -0.2 61,922 - Ac3H11_1119 0.12 -0.1 61,973 + Ac3H11_1119 0.30 +1.1 61,973 + Ac3H11_1119 0.30 -0.2 61,974 - Ac3H11_1119 0.30 +0.4 61,974 - Ac3H11_1119 0.30 +0.4 61,974 - Ac3H11_1119 0.30 +1.2 61,974 - Ac3H11_1119 0.30 -0.3 62,067 - Ac3H11_1119 0.63 -0.4 62,144 + +0.4 62,145 - -0.5 62,145 - -0.2 62,145 - +1.2 62,263 - +0.3 62,550 + Ac3H11_1120 0.20 -0.4 62,551 - Ac3H11_1120 0.20 -0.9 62,551 - Ac3H11_1120 0.20 -0.2
Or see this region's nucleotide sequence