Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse3; 5 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06835 and MMP_RS06840 are separated by 38 nucleotides MMP_RS06840 and MMP_RS06845 are separated by 37 nucleotides MMP_RS06845 and MMP_RS06850 are separated by 37 nucleotides MMP_RS06850 and MMP_RS06855 are separated by 31 nucleotides MMP_RS06855 and MMP_RS06860 are separated by 47 nucleotides MMP_RS06860 and MMP_RS06865 are separated by 25 nucleotides MMP_RS06865 and MMP_RS06870 are separated by 96 nucleotides
MMP_RS06835: MMP_RS06835 - 30S ribosomal protein S9, at 1,308,765 to 1,309,169
_RS06835
MMP_RS06840: MMP_RS06840 - DNA-directed RNA polymerase subunit N, at 1,309,208 to 1,309,414
_RS06840
MMP_RS06845: MMP_RS06845 - tRNA-Pro, at 1,309,452 to 1,309,525
_RS06845
MMP_RS06850: MMP_RS06850 - DNA-directed RNA polymerase subunit K, at 1,309,563 to 1,309,736
_RS06850
MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703
_RS06855
MMP_RS06860: MMP_RS06860 - 4Fe-4S binding protein, at 1,310,751 to 1,310,939
_RS06860
MMP_RS06865: MMP_RS06865 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,310,965 to 1,311,231
_RS06865
MMP_RS06870: MMP_RS06870 - DedA family protein, at 1,311,328 to 1,311,801
_RS06870
Position (kb) 1309
1310
1311 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1309.168 kb on + strand at 1309.387 kb on + strand, within MMP_RS06840 at 1309.407 kb on + strand at 1309.425 kb on + strand at 1309.432 kb on - strand at 1309.436 kb on + strand at 1309.540 kb on + strand at 1309.672 kb on - strand at 1309.742 kb on - strand at 1309.836 kb on + strand at 1309.846 kb on + strand at 1309.870 kb on - strand, within MMP_RS06855 at 1309.925 kb on - strand, within MMP_RS06855 at 1309.957 kb on - strand, within MMP_RS06855 at 1309.985 kb on - strand, within MMP_RS06855 at 1310.195 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.204 kb on + strand, within MMP_RS06855 at 1310.266 kb on + strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.374 kb on + strand, within MMP_RS06855 at 1310.402 kb on - strand, within MMP_RS06855 at 1310.519 kb on + strand, within MMP_RS06855 at 1310.521 kb on - strand, within MMP_RS06855 at 1310.591 kb on - strand, within MMP_RS06855 at 1310.592 kb on + strand, within MMP_RS06855 at 1310.632 kb on + strand at 1310.691 kb on - strand at 1310.721 kb on - strand at 1310.722 kb on + strand at 1310.723 kb on - strand at 1310.723 kb on - strand at 1310.724 kb on + strand at 1310.730 kb on - strand at 1310.851 kb on - strand, within MMP_RS06860 at 1310.881 kb on - strand, within MMP_RS06860 at 1310.937 kb on - strand at 1310.941 kb on - strand at 1311.007 kb on - strand, within MMP_RS06865 at 1311.098 kb on - strand, within MMP_RS06865 at 1311.099 kb on + strand, within MMP_RS06865 at 1311.209 kb on + strand at 1311.258 kb on - strand at 1311.267 kb on - strand at 1311.273 kb on - strand at 1311.295 kb on + strand at 1311.343 kb on + strand at 1311.435 kb on - strand, within MMP_RS06870 at 1311.480 kb on - strand, within MMP_RS06870 at 1311.517 kb on + strand, within MMP_RS06870 at 1311.543 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.602 kb on - strand, within MMP_RS06870 at 1311.608 kb on + strand, within MMP_RS06870
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse3; 5 generations remove 1,309,168 + -1.5 1,309,387 + MMP_RS06840 0.86 +0.5 1,309,407 + +0.0 1,309,425 + -0.1 1,309,432 - -0.6 1,309,436 + +0.0 1,309,540 + -1.3 1,309,672 - -1.8 1,309,742 - -0.5 1,309,836 + -0.7 1,309,846 + -0.6 1,309,870 - MMP_RS06855 0.11 -0.0 1,309,925 - MMP_RS06855 0.17 -0.6 1,309,957 - MMP_RS06855 0.20 -0.8 1,309,985 - MMP_RS06855 0.23 -0.9 1,310,195 - MMP_RS06855 0.46 -0.8 1,310,199 - MMP_RS06855 0.46 -1.4 1,310,199 - MMP_RS06855 0.46 -0.6 1,310,204 + MMP_RS06855 0.47 -0.6 1,310,266 + MMP_RS06855 0.53 -0.9 1,310,319 - MMP_RS06855 0.59 -0.6 1,310,319 - MMP_RS06855 0.59 -0.3 1,310,374 + MMP_RS06855 0.65 -1.0 1,310,402 - MMP_RS06855 0.68 -0.8 1,310,519 + MMP_RS06855 0.80 -0.5 1,310,521 - MMP_RS06855 0.80 -0.5 1,310,591 - MMP_RS06855 0.88 -0.3 1,310,592 + MMP_RS06855 0.88 -0.5 1,310,632 + -0.0 1,310,691 - +0.1 1,310,721 - +0.8 1,310,722 + +0.2 1,310,723 - +0.8 1,310,723 - -0.1 1,310,724 + +0.7 1,310,730 - -0.0 1,310,851 - MMP_RS06860 0.53 +0.0 1,310,881 - MMP_RS06860 0.69 +0.2 1,310,937 - +0.1 1,310,941 - +0.5 1,311,007 - MMP_RS06865 0.16 +0.2 1,311,098 - MMP_RS06865 0.50 -0.7 1,311,099 + MMP_RS06865 0.50 -2.1 1,311,209 + -2.5 1,311,258 - -2.9 1,311,267 - -1.1 1,311,273 - -0.8 1,311,295 + -0.1 1,311,343 + +0.1 1,311,435 - MMP_RS06870 0.23 -0.1 1,311,480 - MMP_RS06870 0.32 -0.2 1,311,517 + MMP_RS06870 0.40 +0.4 1,311,543 + MMP_RS06870 0.45 -0.1 1,311,556 + MMP_RS06870 0.48 -0.0 1,311,556 + MMP_RS06870 0.48 -0.6 1,311,602 - MMP_RS06870 0.58 -0.3 1,311,608 + MMP_RS06870 0.59 -0.1
Or see this region's nucleotide sequence