Experiment: R2A with Nickel (II) chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS08225 and LRK54_RS08230 overlap by 4 nucleotides LRK54_RS08230 and LRK54_RS08235 overlap by 4 nucleotides LRK54_RS08235 and LRK54_RS08240 are separated by 23 nucleotides LRK54_RS08240 and LRK54_RS08245 are separated by 54 nucleotides LRK54_RS08245 and LRK54_RS08250 are separated by 171 nucleotides
LRK54_RS08225: LRK54_RS08225 - AI-2E family transporter, at 1,797,750 to 1,798,940
_RS08225
LRK54_RS08230: LRK54_RS08230 - hypothetical protein, at 1,798,937 to 1,799,260
_RS08230
LRK54_RS08235: LRK54_RS08235 - hypothetical protein, at 1,799,257 to 1,799,742
_RS08235
LRK54_RS08240: LRK54_RS08240 - DUF883 family protein, at 1,799,766 to 1,800,128
_RS08240
LRK54_RS08245: LRK54_RS08245 - DUF1328 domain-containing protein, at 1,800,183 to 1,800,338
_RS08245
LRK54_RS08250: LRK54_RS08250 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 1,800,510 to 1,801,133
_RS08250
Position (kb)
1799
1800 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1798.269 kb on + strand, within LRK54_RS08225 at 1798.269 kb on + strand, within LRK54_RS08225 at 1798.269 kb on + strand, within LRK54_RS08225 at 1798.269 kb on + strand, within LRK54_RS08225 at 1798.270 kb on - strand, within LRK54_RS08225 at 1798.270 kb on - strand, within LRK54_RS08225 at 1798.297 kb on + strand, within LRK54_RS08225 at 1798.297 kb on + strand, within LRK54_RS08225 at 1798.297 kb on + strand, within LRK54_RS08225 at 1798.297 kb on + strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.298 kb on - strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.732 kb on + strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1798.733 kb on - strand, within LRK54_RS08225 at 1799.105 kb on + strand, within LRK54_RS08230 at 1799.105 kb on + strand, within LRK54_RS08230 at 1799.106 kb on - strand, within LRK54_RS08230 at 1799.106 kb on - strand, within LRK54_RS08230 at 1799.106 kb on - strand, within LRK54_RS08230 at 1799.106 kb on - strand, within LRK54_RS08230 at 1799.106 kb on - strand, within LRK54_RS08230 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.845 kb on + strand, within LRK54_RS08240 at 1799.846 kb on - strand, within LRK54_RS08240 at 1799.846 kb on - strand, within LRK54_RS08240 at 1799.846 kb on - strand, within LRK54_RS08240 at 1799.846 kb on - strand, within LRK54_RS08240 at 1799.846 kb on - strand, within LRK54_RS08240 at 1799.853 kb on + strand, within LRK54_RS08240 at 1799.853 kb on + strand, within LRK54_RS08240 at 1799.853 kb on + strand, within LRK54_RS08240 at 1799.853 kb on + strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.854 kb on - strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.896 kb on + strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1799.897 kb on - strand, within LRK54_RS08240 at 1800.328 kb on + strand at 1800.328 kb on + strand at 1800.328 kb on + strand at 1800.328 kb on + strand at 1800.329 kb on - strand at 1800.329 kb on - strand at 1800.329 kb on - strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.384 kb on + strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.385 kb on - strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.538 kb on + strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.539 kb on - strand at 1800.661 kb on + strand, within LRK54_RS08250 at 1800.661 kb on + strand, within LRK54_RS08250 at 1800.661 kb on + strand, within LRK54_RS08250 at 1800.661 kb on + strand, within LRK54_RS08250 at 1800.661 kb on + strand, within LRK54_RS08250 at 1800.662 kb on - strand, within LRK54_RS08250 at 1800.662 kb on - strand, within LRK54_RS08250 at 1800.662 kb on - strand, within LRK54_RS08250 at 1800.707 kb on - strand, within LRK54_RS08250 at 1800.715 kb on + strand, within LRK54_RS08250 at 1800.715 kb on + strand, within LRK54_RS08250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3125 mM remove 1,798,269 + LRK54_RS08225 0.44 +0.0 1,798,269 + LRK54_RS08225 0.44 +1.5 1,798,269 + LRK54_RS08225 0.44 +0.5 1,798,269 + LRK54_RS08225 0.44 +0.4 1,798,270 - LRK54_RS08225 0.44 -0.7 1,798,270 - LRK54_RS08225 0.44 -1.4 1,798,297 + LRK54_RS08225 0.46 -0.2 1,798,297 + LRK54_RS08225 0.46 -0.7 1,798,297 + LRK54_RS08225 0.46 +0.8 1,798,297 + LRK54_RS08225 0.46 -0.8 1,798,298 - LRK54_RS08225 0.46 -1.6 1,798,298 - LRK54_RS08225 0.46 -2.3 1,798,298 - LRK54_RS08225 0.46 +0.5 1,798,298 - LRK54_RS08225 0.46 -2.5 1,798,298 - LRK54_RS08225 0.46 +0.7 1,798,298 - LRK54_RS08225 0.46 +0.9 1,798,732 + LRK54_RS08225 0.82 +0.4 1,798,732 + LRK54_RS08225 0.82 -1.9 1,798,732 + LRK54_RS08225 0.82 +0.5 1,798,732 + LRK54_RS08225 0.82 -1.5 1,798,732 + LRK54_RS08225 0.82 +2.0 1,798,732 + LRK54_RS08225 0.82 -2.6 1,798,732 + LRK54_RS08225 0.82 -0.9 1,798,732 + LRK54_RS08225 0.82 +0.4 1,798,732 + LRK54_RS08225 0.82 -1.6 1,798,732 + LRK54_RS08225 0.82 -0.2 1,798,732 + LRK54_RS08225 0.82 -0.8 1,798,733 - LRK54_RS08225 0.83 -0.8 1,798,733 - LRK54_RS08225 0.83 -0.6 1,798,733 - LRK54_RS08225 0.83 -0.8 1,798,733 - LRK54_RS08225 0.83 -1.3 1,798,733 - LRK54_RS08225 0.83 -1.0 1,798,733 - LRK54_RS08225 0.83 -2.6 1,798,733 - LRK54_RS08225 0.83 -0.4 1,798,733 - LRK54_RS08225 0.83 +0.4 1,799,105 + LRK54_RS08230 0.52 -0.1 1,799,105 + LRK54_RS08230 0.52 +0.6 1,799,106 - LRK54_RS08230 0.52 -2.2 1,799,106 - LRK54_RS08230 0.52 -0.7 1,799,106 - LRK54_RS08230 0.52 -0.1 1,799,106 - LRK54_RS08230 0.52 -1.2 1,799,106 - LRK54_RS08230 0.52 +0.2 1,799,845 + LRK54_RS08240 0.22 -1.7 1,799,845 + LRK54_RS08240 0.22 -2.9 1,799,845 + LRK54_RS08240 0.22 +1.5 1,799,845 + LRK54_RS08240 0.22 -1.9 1,799,845 + LRK54_RS08240 0.22 -0.8 1,799,845 + LRK54_RS08240 0.22 -2.1 1,799,845 + LRK54_RS08240 0.22 -4.2 1,799,845 + LRK54_RS08240 0.22 -3.5 1,799,845 + LRK54_RS08240 0.22 -0.0 1,799,845 + LRK54_RS08240 0.22 -1.3 1,799,846 - LRK54_RS08240 0.22 -1.6 1,799,846 - LRK54_RS08240 0.22 -0.4 1,799,846 - LRK54_RS08240 0.22 -1.8 1,799,846 - LRK54_RS08240 0.22 -0.7 1,799,846 - LRK54_RS08240 0.22 -1.0 1,799,853 + LRK54_RS08240 0.24 -2.0 1,799,853 + LRK54_RS08240 0.24 -1.4 1,799,853 + LRK54_RS08240 0.24 -2.3 1,799,853 + LRK54_RS08240 0.24 -1.9 1,799,854 - LRK54_RS08240 0.24 +0.2 1,799,854 - LRK54_RS08240 0.24 -1.1 1,799,854 - LRK54_RS08240 0.24 +0.9 1,799,854 - LRK54_RS08240 0.24 -1.0 1,799,854 - LRK54_RS08240 0.24 -0.3 1,799,854 - LRK54_RS08240 0.24 +1.9 1,799,854 - LRK54_RS08240 0.24 -1.3 1,799,896 + LRK54_RS08240 0.36 -0.7 1,799,896 + LRK54_RS08240 0.36 -0.1 1,799,896 + LRK54_RS08240 0.36 +0.9 1,799,896 + LRK54_RS08240 0.36 -0.7 1,799,896 + LRK54_RS08240 0.36 -0.7 1,799,896 + LRK54_RS08240 0.36 -1.9 1,799,896 + LRK54_RS08240 0.36 +0.0 1,799,896 + LRK54_RS08240 0.36 -1.5 1,799,896 + LRK54_RS08240 0.36 -2.2 1,799,897 - LRK54_RS08240 0.36 -1.4 1,799,897 - LRK54_RS08240 0.36 -0.2 1,799,897 - LRK54_RS08240 0.36 -0.2 1,799,897 - LRK54_RS08240 0.36 -1.3 1,799,897 - LRK54_RS08240 0.36 -0.5 1,799,897 - LRK54_RS08240 0.36 -0.5 1,799,897 - LRK54_RS08240 0.36 +1.0 1,799,897 - LRK54_RS08240 0.36 -1.1 1,799,897 - LRK54_RS08240 0.36 +0.3 1,799,897 - LRK54_RS08240 0.36 +1.8 1,800,328 + -0.5 1,800,328 + -2.6 1,800,328 + -0.8 1,800,328 + -1.1 1,800,329 - +0.9 1,800,329 - +1.5 1,800,329 - +0.5 1,800,384 + +0.2 1,800,384 + -0.1 1,800,384 + -3.6 1,800,384 + -1.5 1,800,384 + +0.8 1,800,384 + -0.4 1,800,384 + +1.0 1,800,384 + +1.0 1,800,385 - -0.2 1,800,385 - +1.4 1,800,385 - +0.9 1,800,385 - -1.5 1,800,385 - +2.2 1,800,385 - -0.0 1,800,385 - +0.8 1,800,385 - -0.4 1,800,385 - -1.9 1,800,385 - -2.2 1,800,538 + +0.5 1,800,538 + -1.0 1,800,538 + +2.4 1,800,538 + +1.1 1,800,538 + -1.4 1,800,538 + +0.8 1,800,538 + -0.4 1,800,538 + -0.6 1,800,538 + -0.9 1,800,539 - +1.1 1,800,539 - -1.4 1,800,539 - -0.7 1,800,539 - +0.3 1,800,539 - -0.2 1,800,539 - +0.1 1,800,539 - -0.8 1,800,539 - +2.5 1,800,539 - -1.6 1,800,539 - -2.0 1,800,661 + LRK54_RS08250 0.24 -1.1 1,800,661 + LRK54_RS08250 0.24 +0.4 1,800,661 + LRK54_RS08250 0.24 -1.9 1,800,661 + LRK54_RS08250 0.24 +1.0 1,800,661 + LRK54_RS08250 0.24 -1.0 1,800,662 - LRK54_RS08250 0.24 +0.5 1,800,662 - LRK54_RS08250 0.24 -1.0 1,800,662 - LRK54_RS08250 0.24 -2.4 1,800,707 - LRK54_RS08250 0.32 -0.3 1,800,715 + LRK54_RS08250 0.33 -2.5 1,800,715 + LRK54_RS08250 0.33 -0.1
Or see this region's nucleotide sequence