Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20830 and EX31_RS26100 are separated by 164 nucleotides EX31_RS26100 and EX31_RS20835 are separated by 70 nucleotides EX31_RS20835 and EX31_RS20840 are separated by 36 nucleotides EX31_RS20840 and EX31_RS20845 are separated by 32 nucleotides EX31_RS20845 and EX31_RS25590 overlap by 1 nucleotides
EX31_RS20830: EX31_RS20830 - LysR family transcriptional regulator, at 815,202 to 816,095
_RS20830
EX31_RS26100: EX31_RS26100 - AraC family transcriptional regulator, at 816,260 to 816,334
_RS26100
EX31_RS20835: EX31_RS20835 - NIPSNAP family containing protein, at 816,405 to 816,731
_RS20835
EX31_RS20840: EX31_RS20840 - hypothetical protein, at 816,768 to 816,989
_RS20840
EX31_RS20845: EX31_RS20845 - hypothetical protein, at 817,022 to 817,273
_RS20845
EX31_RS25590: EX31_RS25590 - hypothetical protein, at 817,273 to 817,407
_RS25590
Position (kb) 816
817 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 815.269 kb on + strand at 815.270 kb on - strand at 815.270 kb on - strand at 815.270 kb on - strand at 815.272 kb on + strand at 815.272 kb on + strand at 815.273 kb on - strand at 815.278 kb on + strand at 815.278 kb on + strand at 815.337 kb on + strand, within EX31_RS20830 at 815.338 kb on - strand, within EX31_RS20830 at 815.339 kb on + strand, within EX31_RS20830 at 815.339 kb on + strand, within EX31_RS20830 at 815.339 kb on + strand, within EX31_RS20830 at 815.339 kb on + strand, within EX31_RS20830 at 815.339 kb on + strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.340 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.342 kb on - strand, within EX31_RS20830 at 815.349 kb on + strand, within EX31_RS20830 at 815.364 kb on + strand, within EX31_RS20830 at 815.364 kb on + strand, within EX31_RS20830 at 815.364 kb on + strand, within EX31_RS20830 at 815.365 kb on - strand, within EX31_RS20830 at 815.365 kb on - strand, within EX31_RS20830 at 815.366 kb on + strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.367 kb on - strand, within EX31_RS20830 at 815.389 kb on - strand, within EX31_RS20830 at 815.421 kb on - strand, within EX31_RS20830 at 815.421 kb on - strand, within EX31_RS20830 at 815.514 kb on - strand, within EX31_RS20830 at 815.536 kb on - strand, within EX31_RS20830 at 815.575 kb on + strand, within EX31_RS20830 at 815.575 kb on + strand, within EX31_RS20830 at 815.576 kb on - strand, within EX31_RS20830 at 815.691 kb on - strand, within EX31_RS20830 at 815.691 kb on - strand, within EX31_RS20830 at 815.727 kb on - strand, within EX31_RS20830 at 815.802 kb on + strand, within EX31_RS20830 at 815.810 kb on + strand, within EX31_RS20830 at 815.810 kb on + strand, within EX31_RS20830 at 815.811 kb on - strand, within EX31_RS20830 at 815.811 kb on - strand, within EX31_RS20830 at 815.895 kb on - strand, within EX31_RS20830 at 815.895 kb on - strand, within EX31_RS20830 at 816.052 kb on - strand at 816.127 kb on + strand at 816.128 kb on - strand at 816.128 kb on - strand at 816.190 kb on + strand at 816.203 kb on - strand at 816.217 kb on + strand at 816.217 kb on + strand at 816.218 kb on - strand at 816.218 kb on - strand at 816.229 kb on - strand at 816.282 kb on + strand, within EX31_RS26100 at 816.282 kb on + strand, within EX31_RS26100 at 816.283 kb on - strand, within EX31_RS26100 at 816.283 kb on - strand, within EX31_RS26100 at 816.287 kb on - strand, within EX31_RS26100 at 816.339 kb on + strand at 816.339 kb on + strand at 816.339 kb on + strand at 816.340 kb on - strand at 816.373 kb on + strand at 816.389 kb on - strand at 816.390 kb on + strand at 816.398 kb on + strand at 816.399 kb on - strand at 816.429 kb on + strand at 816.429 kb on + strand at 816.430 kb on - strand at 816.430 kb on - strand at 816.430 kb on - strand at 816.436 kb on + strand at 816.437 kb on - strand at 816.555 kb on + strand, within EX31_RS20835 at 816.555 kb on + strand, within EX31_RS20835 at 816.555 kb on + strand, within EX31_RS20835 at 816.556 kb on - strand, within EX31_RS20835 at 816.558 kb on + strand, within EX31_RS20835 at 816.558 kb on + strand, within EX31_RS20835 at 816.558 kb on + strand, within EX31_RS20835 at 816.559 kb on - strand, within EX31_RS20835 at 816.559 kb on - strand, within EX31_RS20835 at 816.559 kb on - strand, within EX31_RS20835 at 816.559 kb on - strand, within EX31_RS20835 at 816.559 kb on - strand, within EX31_RS20835 at 816.615 kb on + strand, within EX31_RS20835 at 816.615 kb on + strand, within EX31_RS20835 at 816.615 kb on + strand, within EX31_RS20835 at 816.615 kb on + strand, within EX31_RS20835 at 816.615 kb on + strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.616 kb on - strand, within EX31_RS20835 at 816.662 kb on + strand, within EX31_RS20835 at 816.662 kb on + strand, within EX31_RS20835 at 816.662 kb on + strand, within EX31_RS20835 at 816.663 kb on - strand, within EX31_RS20835 at 816.680 kb on + strand, within EX31_RS20835 at 816.680 kb on + strand, within EX31_RS20835 at 816.729 kb on + strand at 816.748 kb on + strand at 816.748 kb on + strand at 816.750 kb on + strand at 816.750 kb on + strand at 816.750 kb on + strand at 816.750 kb on + strand at 816.750 kb on + strand at 816.750 kb on + strand at 816.751 kb on - strand at 816.751 kb on - strand at 816.751 kb on - strand at 816.755 kb on + strand at 816.755 kb on + strand at 816.755 kb on + strand at 816.755 kb on + strand at 816.756 kb on - strand at 816.758 kb on + strand at 816.764 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.785 kb on - strand at 816.785 kb on - strand at 816.911 kb on - strand, within EX31_RS20840 at 816.911 kb on - strand, within EX31_RS20840 at 816.914 kb on + strand, within EX31_RS20840 at 816.915 kb on - strand, within EX31_RS20840 at 816.993 kb on + strand at 816.994 kb on - strand at 816.995 kb on + strand at 817.000 kb on + strand at 817.001 kb on - strand at 817.007 kb on + strand at 817.007 kb on + strand at 817.023 kb on + strand at 817.098 kb on - strand, within EX31_RS20845 at 817.114 kb on + strand, within EX31_RS20845 at 817.142 kb on - strand, within EX31_RS20845 at 817.153 kb on + strand, within EX31_RS20845 at 817.154 kb on - strand, within EX31_RS20845 at 817.199 kb on - strand, within EX31_RS20845 at 817.199 kb on - strand, within EX31_RS20845 at 817.215 kb on - strand, within EX31_RS20845 at 817.226 kb on + strand, within EX31_RS20845 at 817.226 kb on + strand, within EX31_RS20845 at 817.261 kb on + strand at 817.261 kb on + strand at 817.262 kb on - strand at 817.262 kb on - strand at 817.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 815,269 + +0.1 815,270 - +2.1 815,270 - +1.0 815,270 - +0.5 815,272 + +0.5 815,272 + +0.1 815,273 - -0.3 815,278 + +0.8 815,278 + +0.8 815,337 + EX31_RS20830 0.15 -1.9 815,338 - EX31_RS20830 0.15 -0.6 815,339 + EX31_RS20830 0.15 +0.1 815,339 + EX31_RS20830 0.15 +1.5 815,339 + EX31_RS20830 0.15 +0.8 815,339 + EX31_RS20830 0.15 +0.5 815,339 + EX31_RS20830 0.15 -0.9 815,340 - EX31_RS20830 0.15 -0.2 815,340 - EX31_RS20830 0.15 +0.8 815,340 - EX31_RS20830 0.15 +0.2 815,340 - EX31_RS20830 0.15 -1.3 815,340 - EX31_RS20830 0.15 +0.5 815,340 - EX31_RS20830 0.15 +0.2 815,340 - EX31_RS20830 0.15 +0.7 815,340 - EX31_RS20830 0.15 -0.6 815,342 - EX31_RS20830 0.16 +1.5 815,342 - EX31_RS20830 0.16 -0.5 815,342 - EX31_RS20830 0.16 +1.5 815,342 - EX31_RS20830 0.16 -0.2 815,342 - EX31_RS20830 0.16 +0.6 815,342 - EX31_RS20830 0.16 +1.0 815,349 + EX31_RS20830 0.16 +0.7 815,364 + EX31_RS20830 0.18 -1.2 815,364 + EX31_RS20830 0.18 +0.3 815,364 + EX31_RS20830 0.18 -1.2 815,365 - EX31_RS20830 0.18 -0.2 815,365 - EX31_RS20830 0.18 +0.4 815,366 + EX31_RS20830 0.18 +0.2 815,367 - EX31_RS20830 0.18 +0.2 815,367 - EX31_RS20830 0.18 +0.3 815,367 - EX31_RS20830 0.18 -1.2 815,367 - EX31_RS20830 0.18 +0.5 815,367 - EX31_RS20830 0.18 +0.4 815,367 - EX31_RS20830 0.18 +0.7 815,389 - EX31_RS20830 0.21 -0.3 815,421 - EX31_RS20830 0.24 -0.3 815,421 - EX31_RS20830 0.24 +0.3 815,514 - EX31_RS20830 0.35 -0.8 815,536 - EX31_RS20830 0.37 -0.2 815,575 + EX31_RS20830 0.42 +0.3 815,575 + EX31_RS20830 0.42 -0.0 815,576 - EX31_RS20830 0.42 +0.0 815,691 - EX31_RS20830 0.55 -0.6 815,691 - EX31_RS20830 0.55 +2.3 815,727 - EX31_RS20830 0.59 +0.9 815,802 + EX31_RS20830 0.67 +0.7 815,810 + EX31_RS20830 0.68 -1.6 815,810 + EX31_RS20830 0.68 +0.3 815,811 - EX31_RS20830 0.68 -0.3 815,811 - EX31_RS20830 0.68 -0.2 815,895 - EX31_RS20830 0.78 +0.1 815,895 - EX31_RS20830 0.78 +1.1 816,052 - +0.5 816,127 + -1.7 816,128 - -0.4 816,128 - +1.4 816,190 + +1.1 816,203 - -0.0 816,217 + -0.0 816,217 + -0.2 816,218 - -0.2 816,218 - +0.9 816,229 - -0.9 816,282 + EX31_RS26100 0.29 +0.2 816,282 + EX31_RS26100 0.29 -1.2 816,283 - EX31_RS26100 0.31 -0.6 816,283 - EX31_RS26100 0.31 +0.2 816,287 - EX31_RS26100 0.36 -0.4 816,339 + +0.8 816,339 + +0.4 816,339 + -0.4 816,340 - +1.8 816,373 + -1.1 816,389 - -0.5 816,390 + -0.5 816,398 + +0.6 816,399 - -0.6 816,429 + -1.2 816,429 + -0.6 816,430 - +0.3 816,430 - +1.6 816,430 - -0.7 816,436 + -1.1 816,437 - -2.1 816,555 + EX31_RS20835 0.46 +0.0 816,555 + EX31_RS20835 0.46 +0.1 816,555 + EX31_RS20835 0.46 -0.7 816,556 - EX31_RS20835 0.46 -0.6 816,558 + EX31_RS20835 0.47 -0.1 816,558 + EX31_RS20835 0.47 -0.3 816,558 + EX31_RS20835 0.47 +0.1 816,559 - EX31_RS20835 0.47 +1.8 816,559 - EX31_RS20835 0.47 +0.3 816,559 - EX31_RS20835 0.47 +0.6 816,559 - EX31_RS20835 0.47 +2.8 816,559 - EX31_RS20835 0.47 -1.8 816,615 + EX31_RS20835 0.64 -1.9 816,615 + EX31_RS20835 0.64 -0.6 816,615 + EX31_RS20835 0.64 -0.1 816,615 + EX31_RS20835 0.64 -0.9 816,615 + EX31_RS20835 0.64 -1.2 816,616 - EX31_RS20835 0.65 +0.5 816,616 - EX31_RS20835 0.65 +0.7 816,616 - EX31_RS20835 0.65 +1.3 816,616 - EX31_RS20835 0.65 +0.6 816,616 - EX31_RS20835 0.65 -0.4 816,616 - EX31_RS20835 0.65 +1.2 816,616 - EX31_RS20835 0.65 -0.4 816,662 + EX31_RS20835 0.79 +0.5 816,662 + EX31_RS20835 0.79 -0.6 816,662 + EX31_RS20835 0.79 -0.3 816,663 - EX31_RS20835 0.79 +0.6 816,680 + EX31_RS20835 0.84 -0.2 816,680 + EX31_RS20835 0.84 +0.3 816,729 + +0.4 816,748 + +0.1 816,748 + -0.6 816,750 + -0.7 816,750 + +2.1 816,750 + -0.5 816,750 + +1.4 816,750 + +1.1 816,750 + -0.2 816,751 - +0.6 816,751 - +0.2 816,751 - +1.1 816,755 + +0.7 816,755 + -0.6 816,755 + -1.2 816,755 + -0.1 816,756 - -0.2 816,758 + -0.1 816,764 + -0.5 816,784 + +0.4 816,784 + +0.2 816,784 + +0.3 816,784 + +0.5 816,785 - -0.6 816,785 - +1.1 816,911 - EX31_RS20840 0.64 +0.5 816,911 - EX31_RS20840 0.64 -0.1 816,914 + EX31_RS20840 0.66 +1.1 816,915 - EX31_RS20840 0.66 +0.3 816,993 + -1.2 816,994 - +1.2 816,995 + -0.0 817,000 + +0.9 817,001 - -2.2 817,007 + -0.9 817,007 + +1.5 817,023 + +0.7 817,098 - EX31_RS20845 0.30 +2.1 817,114 + EX31_RS20845 0.37 -0.4 817,142 - EX31_RS20845 0.48 +0.2 817,153 + EX31_RS20845 0.52 -1.6 817,154 - EX31_RS20845 0.52 -1.7 817,199 - EX31_RS20845 0.70 +1.0 817,199 - EX31_RS20845 0.70 +0.3 817,215 - EX31_RS20845 0.77 -0.3 817,226 + EX31_RS20845 0.81 +0.3 817,226 + EX31_RS20845 0.81 +1.0 817,261 + -0.1 817,261 + -0.1 817,262 - +0.6 817,262 - -0.5 817,275 - -0.4
Or see this region's nucleotide sequence