Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22330 and EX31_RS22335 are separated by 67 nucleotides EX31_RS22335 and EX31_RS22340 are separated by 396 nucleotides EX31_RS22340 and EX31_RS22345 are separated by 51 nucleotides EX31_RS22345 and EX31_RS22350 are separated by 112 nucleotides
EX31_RS22330: EX31_RS22330 - hypothetical protein, at 219,689 to 220,270
_RS22330
EX31_RS22335: EX31_RS22335 - MGMT family protein, at 220,338 to 220,646
_RS22335
EX31_RS22340: EX31_RS22340 - site-specific integrase, at 221,043 to 222,134
_RS22340
EX31_RS22345: EX31_RS22345 - hypothetical protein, at 222,186 to 222,998
_RS22345
EX31_RS22350: EX31_RS22350 - hypothetical protein, at 223,111 to 223,578
_RS22350
Position (kb) 221
222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 220.118 kb on - strand, within EX31_RS22330 at 220.241 kb on - strand at 220.266 kb on - strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.269 kb on - strand at 220.269 kb on - strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.289 kb on + strand at 220.290 kb on - strand at 220.354 kb on + strand at 220.355 kb on - strand at 220.383 kb on - strand, within EX31_RS22335 at 220.424 kb on + strand, within EX31_RS22335 at 220.513 kb on - strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.571 kb on - strand, within EX31_RS22335 at 220.571 kb on - strand, within EX31_RS22335 at 220.614 kb on + strand, within EX31_RS22335 at 220.615 kb on - strand, within EX31_RS22335 at 220.674 kb on + strand at 220.674 kb on + strand at 220.675 kb on - strand at 220.699 kb on - strand at 220.716 kb on + strand at 220.716 kb on + strand at 220.717 kb on - strand at 220.748 kb on + strand at 220.748 kb on + strand at 220.749 kb on - strand at 220.801 kb on + strand at 220.974 kb on + strand at 221.009 kb on + strand at 221.010 kb on - strand at 221.022 kb on + strand at 221.022 kb on + strand at 221.029 kb on + strand at 221.030 kb on - strand at 221.030 kb on - strand at 221.054 kb on + strand at 221.054 kb on + strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.079 kb on + strand at 221.079 kb on + strand at 221.095 kb on - strand at 221.179 kb on - strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.306 kb on + strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.307 kb on - strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.310 kb on + strand, within EX31_RS22340 at 221.311 kb on - strand, within EX31_RS22340 at 221.311 kb on - strand, within EX31_RS22340 at 221.371 kb on + strand, within EX31_RS22340 at 221.371 kb on + strand, within EX31_RS22340 at 221.371 kb on + strand, within EX31_RS22340 at 221.371 kb on + strand, within EX31_RS22340 at 221.371 kb on + strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.372 kb on - strand, within EX31_RS22340 at 221.379 kb on + strand, within EX31_RS22340 at 221.380 kb on - strand, within EX31_RS22340 at 221.469 kb on + strand, within EX31_RS22340 at 221.593 kb on - strand, within EX31_RS22340 at 221.609 kb on + strand, within EX31_RS22340 at 221.609 kb on + strand, within EX31_RS22340 at 221.609 kb on + strand, within EX31_RS22340 at 221.617 kb on + strand, within EX31_RS22340 at 221.617 kb on + strand, within EX31_RS22340 at 221.618 kb on - strand, within EX31_RS22340 at 221.618 kb on - strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.626 kb on + strand, within EX31_RS22340 at 221.627 kb on - strand, within EX31_RS22340 at 221.627 kb on - strand, within EX31_RS22340 at 221.627 kb on - strand, within EX31_RS22340 at 221.679 kb on - strand, within EX31_RS22340 at 221.679 kb on - strand, within EX31_RS22340 at 221.684 kb on + strand, within EX31_RS22340 at 221.743 kb on + strand, within EX31_RS22340 at 221.744 kb on - strand, within EX31_RS22340 at 221.744 kb on - strand, within EX31_RS22340 at 221.762 kb on + strand, within EX31_RS22340 at 221.763 kb on - strand, within EX31_RS22340 at 221.830 kb on + strand, within EX31_RS22340 at 221.890 kb on + strand, within EX31_RS22340 at 221.902 kb on + strand, within EX31_RS22340 at 221.903 kb on - strand, within EX31_RS22340 at 221.910 kb on + strand, within EX31_RS22340 at 221.910 kb on + strand, within EX31_RS22340 at 221.910 kb on + strand, within EX31_RS22340 at 221.911 kb on - strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.955 kb on + strand, within EX31_RS22340 at 221.956 kb on - strand, within EX31_RS22340 at 221.956 kb on - strand, within EX31_RS22340 at 221.970 kb on + strand, within EX31_RS22340 at 222.072 kb on + strand at 222.072 kb on + strand at 222.072 kb on + strand at 222.073 kb on - strand at 222.073 kb on - strand at 222.073 kb on - strand at 222.073 kb on - strand at 222.143 kb on - strand at 222.143 kb on - strand at 222.143 kb on - strand at 222.148 kb on - strand at 222.148 kb on - strand at 222.149 kb on + strand at 222.150 kb on - strand at 222.152 kb on + strand at 222.152 kb on + strand at 222.152 kb on + strand at 222.152 kb on + strand at 222.153 kb on - strand at 222.153 kb on - strand at 222.153 kb on - strand at 222.179 kb on + strand at 222.213 kb on + strand at 222.219 kb on - strand at 222.226 kb on + strand at 222.248 kb on + strand at 222.314 kb on + strand, within EX31_RS22345 at 222.314 kb on + strand, within EX31_RS22345 at 222.329 kb on + strand, within EX31_RS22345 at 222.329 kb on + strand, within EX31_RS22345 at 222.386 kb on - strand, within EX31_RS22345 at 222.391 kb on + strand, within EX31_RS22345 at 222.391 kb on + strand, within EX31_RS22345 at 222.392 kb on - strand, within EX31_RS22345 at 222.392 kb on - strand, within EX31_RS22345 at 222.392 kb on - strand, within EX31_RS22345 at 222.392 kb on - strand, within EX31_RS22345 at 222.395 kb on + strand, within EX31_RS22345 at 222.395 kb on + strand, within EX31_RS22345 at 222.425 kb on + strand, within EX31_RS22345 at 222.440 kb on + strand, within EX31_RS22345 at 222.499 kb on + strand, within EX31_RS22345 at 222.500 kb on - strand, within EX31_RS22345 at 222.500 kb on - strand, within EX31_RS22345 at 222.517 kb on + strand, within EX31_RS22345 at 222.545 kb on - strand, within EX31_RS22345 at 222.558 kb on - strand, within EX31_RS22345 at 222.605 kb on - strand, within EX31_RS22345 at 222.605 kb on - strand, within EX31_RS22345 at 222.605 kb on - strand, within EX31_RS22345 at 222.605 kb on - strand, within EX31_RS22345 at 222.605 kb on - strand, within EX31_RS22345 at 222.633 kb on - strand, within EX31_RS22345 at 222.643 kb on + strand, within EX31_RS22345 at 222.734 kb on + strand, within EX31_RS22345 at 222.744 kb on - strand, within EX31_RS22345 at 222.744 kb on - strand, within EX31_RS22345 at 222.745 kb on + strand, within EX31_RS22345 at 222.746 kb on - strand, within EX31_RS22345 at 222.746 kb on - strand, within EX31_RS22345 at 222.937 kb on + strand at 222.937 kb on + strand at 222.937 kb on + strand at 222.938 kb on - strand at 222.938 kb on - strand at 222.938 kb on - strand at 222.942 kb on - strand at 222.950 kb on + strand at 222.950 kb on + strand at 222.951 kb on - strand at 222.973 kb on + strand at 222.973 kb on + strand at 222.973 kb on + strand at 223.053 kb on + strand at 223.054 kb on - strand at 223.072 kb on + strand at 223.072 kb on + strand at 223.072 kb on + strand at 223.086 kb on + strand at 223.086 kb on + strand at 223.086 kb on + strand at 223.132 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 220,118 - EX31_RS22330 0.74 +0.1 220,241 - -1.7 220,266 - -0.3 220,268 + -1.3 220,268 + +1.1 220,268 + +0.7 220,269 - +0.9 220,269 - +0.7 220,280 + +0.4 220,280 + -0.1 220,280 + -0.4 220,280 + +1.0 220,280 + +0.1 220,280 + -0.0 220,280 + -1.5 220,280 + +1.1 220,280 + -0.1 220,280 + -0.5 220,280 + +0.8 220,280 + +0.0 220,280 + +0.7 220,280 + -0.9 220,281 - +0.2 220,281 - +0.8 220,281 - +0.4 220,281 - +0.6 220,281 - -0.1 220,281 - -0.5 220,281 - -1.2 220,281 - +1.9 220,281 - +0.1 220,281 - -0.2 220,281 - +0.3 220,281 - +0.1 220,289 + +0.6 220,290 - +0.6 220,354 + +0.3 220,355 - -1.2 220,383 - EX31_RS22335 0.15 -0.0 220,424 + EX31_RS22335 0.28 -1.2 220,513 - EX31_RS22335 0.57 +1.6 220,570 + EX31_RS22335 0.75 -0.2 220,570 + EX31_RS22335 0.75 +0.2 220,570 + EX31_RS22335 0.75 -0.7 220,570 + EX31_RS22335 0.75 +0.1 220,571 - EX31_RS22335 0.75 +1.3 220,571 - EX31_RS22335 0.75 -0.3 220,614 + EX31_RS22335 0.89 -0.8 220,615 - EX31_RS22335 0.90 -0.3 220,674 + +0.2 220,674 + +0.6 220,675 - +0.1 220,699 - -0.5 220,716 + +0.5 220,716 + +0.4 220,717 - -0.8 220,748 + +0.3 220,748 + +0.2 220,749 - +1.1 220,801 + +1.5 220,974 + -3.0 221,009 + +0.2 221,010 - +1.1 221,022 + -0.6 221,022 + +0.2 221,029 + +0.4 221,030 - -0.4 221,030 - +0.9 221,054 + -1.0 221,054 + -0.7 221,055 - +1.1 221,055 - -0.0 221,055 - -0.8 221,055 - +0.3 221,055 - +0.5 221,055 - +0.9 221,079 + -0.3 221,079 + -1.1 221,095 - +1.7 221,179 - EX31_RS22340 0.12 +1.1 221,306 + EX31_RS22340 0.24 +0.1 221,306 + EX31_RS22340 0.24 +0.9 221,306 + EX31_RS22340 0.24 +1.8 221,306 + EX31_RS22340 0.24 -0.4 221,306 + EX31_RS22340 0.24 +1.0 221,306 + EX31_RS22340 0.24 +0.3 221,306 + EX31_RS22340 0.24 +0.9 221,306 + EX31_RS22340 0.24 -0.7 221,307 - EX31_RS22340 0.24 +1.2 221,307 - EX31_RS22340 0.24 +1.0 221,307 - EX31_RS22340 0.24 -0.9 221,307 - EX31_RS22340 0.24 +0.7 221,307 - EX31_RS22340 0.24 -0.9 221,307 - EX31_RS22340 0.24 +2.5 221,307 - EX31_RS22340 0.24 +1.0 221,307 - EX31_RS22340 0.24 +0.8 221,307 - EX31_RS22340 0.24 -0.4 221,310 + EX31_RS22340 0.24 -0.5 221,310 + EX31_RS22340 0.24 +1.0 221,310 + EX31_RS22340 0.24 -1.6 221,310 + EX31_RS22340 0.24 +0.4 221,310 + EX31_RS22340 0.24 -1.4 221,310 + EX31_RS22340 0.24 +1.9 221,311 - EX31_RS22340 0.25 -0.1 221,311 - EX31_RS22340 0.25 +0.7 221,371 + EX31_RS22340 0.30 +1.2 221,371 + EX31_RS22340 0.30 -0.7 221,371 + EX31_RS22340 0.30 -0.1 221,371 + EX31_RS22340 0.30 -1.4 221,371 + EX31_RS22340 0.30 +0.3 221,372 - EX31_RS22340 0.30 +0.2 221,372 - EX31_RS22340 0.30 -0.5 221,372 - EX31_RS22340 0.30 +0.9 221,372 - EX31_RS22340 0.30 -0.5 221,372 - EX31_RS22340 0.30 +0.1 221,372 - EX31_RS22340 0.30 -1.3 221,372 - EX31_RS22340 0.30 -0.4 221,379 + EX31_RS22340 0.31 +1.2 221,380 - EX31_RS22340 0.31 +1.5 221,469 + EX31_RS22340 0.39 +0.6 221,593 - EX31_RS22340 0.50 +1.0 221,609 + EX31_RS22340 0.52 -0.6 221,609 + EX31_RS22340 0.52 +1.0 221,609 + EX31_RS22340 0.52 -0.0 221,617 + EX31_RS22340 0.53 +0.4 221,617 + EX31_RS22340 0.53 +0.2 221,618 - EX31_RS22340 0.53 -0.4 221,618 - EX31_RS22340 0.53 +0.4 221,626 + EX31_RS22340 0.53 -0.4 221,626 + EX31_RS22340 0.53 +0.2 221,626 + EX31_RS22340 0.53 +1.6 221,626 + EX31_RS22340 0.53 +0.3 221,626 + EX31_RS22340 0.53 -0.2 221,626 + EX31_RS22340 0.53 -2.4 221,626 + EX31_RS22340 0.53 -0.3 221,626 + EX31_RS22340 0.53 -0.2 221,627 - EX31_RS22340 0.53 +0.9 221,627 - EX31_RS22340 0.53 +0.3 221,627 - EX31_RS22340 0.53 +1.2 221,679 - EX31_RS22340 0.58 -1.0 221,679 - EX31_RS22340 0.58 -1.9 221,684 + EX31_RS22340 0.59 +0.1 221,743 + EX31_RS22340 0.64 +0.5 221,744 - EX31_RS22340 0.64 +0.1 221,744 - EX31_RS22340 0.64 +2.8 221,762 + EX31_RS22340 0.66 +0.9 221,763 - EX31_RS22340 0.66 +0.4 221,830 + EX31_RS22340 0.72 +1.3 221,890 + EX31_RS22340 0.78 -0.3 221,902 + EX31_RS22340 0.79 +0.4 221,903 - EX31_RS22340 0.79 +0.9 221,910 + EX31_RS22340 0.79 +0.7 221,910 + EX31_RS22340 0.79 +0.4 221,910 + EX31_RS22340 0.79 -0.2 221,911 - EX31_RS22340 0.79 -0.2 221,955 + EX31_RS22340 0.84 +1.1 221,955 + EX31_RS22340 0.84 -0.6 221,955 + EX31_RS22340 0.84 -0.7 221,955 + EX31_RS22340 0.84 +0.6 221,955 + EX31_RS22340 0.84 -0.2 221,955 + EX31_RS22340 0.84 +0.8 221,955 + EX31_RS22340 0.84 +0.8 221,955 + EX31_RS22340 0.84 -1.6 221,956 - EX31_RS22340 0.84 +0.7 221,956 - EX31_RS22340 0.84 -1.0 221,970 + EX31_RS22340 0.85 +1.9 222,072 + -1.7 222,072 + -0.1 222,072 + -0.8 222,073 - -0.0 222,073 - +1.3 222,073 - -0.5 222,073 - +0.3 222,143 - -0.0 222,143 - +0.9 222,143 - -0.4 222,148 - +0.5 222,148 - +1.3 222,149 + +3.1 222,150 - +1.6 222,152 + -0.4 222,152 + -0.1 222,152 + +0.1 222,152 + +0.1 222,153 - -0.2 222,153 - +0.5 222,153 - +0.3 222,179 + +0.3 222,213 + +1.9 222,219 - -0.2 222,226 + +0.3 222,248 + +0.9 222,314 + EX31_RS22345 0.16 +1.1 222,314 + EX31_RS22345 0.16 -0.7 222,329 + EX31_RS22345 0.18 +0.5 222,329 + EX31_RS22345 0.18 -1.9 222,386 - EX31_RS22345 0.25 -0.2 222,391 + EX31_RS22345 0.25 -0.5 222,391 + EX31_RS22345 0.25 -0.6 222,392 - EX31_RS22345 0.25 +2.3 222,392 - EX31_RS22345 0.25 +1.0 222,392 - EX31_RS22345 0.25 +1.9 222,392 - EX31_RS22345 0.25 -0.4 222,395 + EX31_RS22345 0.26 -1.5 222,395 + EX31_RS22345 0.26 +1.1 222,425 + EX31_RS22345 0.29 -0.4 222,440 + EX31_RS22345 0.31 +0.1 222,499 + EX31_RS22345 0.38 -0.2 222,500 - EX31_RS22345 0.39 -0.7 222,500 - EX31_RS22345 0.39 +0.1 222,517 + EX31_RS22345 0.41 +0.2 222,545 - EX31_RS22345 0.44 +1.0 222,558 - EX31_RS22345 0.46 -0.7 222,605 - EX31_RS22345 0.52 +0.5 222,605 - EX31_RS22345 0.52 -0.0 222,605 - EX31_RS22345 0.52 +0.3 222,605 - EX31_RS22345 0.52 +0.3 222,605 - EX31_RS22345 0.52 +0.7 222,633 - EX31_RS22345 0.55 -0.1 222,643 + EX31_RS22345 0.56 +0.7 222,734 + EX31_RS22345 0.67 +0.2 222,744 - EX31_RS22345 0.69 +0.2 222,744 - EX31_RS22345 0.69 +0.9 222,745 + EX31_RS22345 0.69 -2.4 222,746 - EX31_RS22345 0.69 -1.1 222,746 - EX31_RS22345 0.69 +0.6 222,937 + -1.0 222,937 + +2.3 222,937 + +0.5 222,938 - -0.9 222,938 - +0.2 222,938 - +0.4 222,942 - -0.1 222,950 + +0.3 222,950 + +0.5 222,951 - +1.1 222,973 + -1.1 222,973 + +0.2 222,973 + +0.3 223,053 + +1.0 223,054 - +0.3 223,072 + +1.1 223,072 + +0.9 223,072 + +0.6 223,086 + -0.1 223,086 + -0.9 223,086 + -1.8 223,132 + -0.2
Or see this region's nucleotide sequence