Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21125 and EX31_RS21130 are separated by 198 nucleotides EX31_RS21130 and EX31_RS21135 are separated by 36 nucleotides EX31_RS21135 and EX31_RS21140 overlap by 4 nucleotides
EX31_RS21125: EX31_RS21125 - MurR/RpiR family transcriptional regulator, at 868,352 to 869,191
_RS21125
EX31_RS21130: EX31_RS21130 - gamma-glutamyltransferase, at 869,390 to 870,976
_RS21130
EX31_RS21135: EX31_RS21135 - oxalurate catabolism protein HpxX, at 871,013 to 871,207
_RS21135
EX31_RS21140: EX31_RS21140 - AtzE family amidohydrolase, at 871,204 to 872,607
_RS21140
Position (kb) 869
870
871 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 868.395 kb on - strand at 868.395 kb on - strand at 868.405 kb on + strand at 868.405 kb on + strand at 868.405 kb on + strand at 868.406 kb on - strand at 868.406 kb on - strand at 868.406 kb on - strand at 868.406 kb on - strand at 868.429 kb on + strand at 868.429 kb on + strand at 868.430 kb on - strand at 868.430 kb on - strand at 868.482 kb on + strand, within EX31_RS21125 at 868.482 kb on + strand, within EX31_RS21125 at 868.482 kb on + strand, within EX31_RS21125 at 868.482 kb on + strand, within EX31_RS21125 at 868.482 kb on + strand, within EX31_RS21125 at 868.483 kb on - strand, within EX31_RS21125 at 868.483 kb on - strand, within EX31_RS21125 at 868.483 kb on - strand, within EX31_RS21125 at 868.543 kb on + strand, within EX31_RS21125 at 868.543 kb on + strand, within EX31_RS21125 at 868.544 kb on - strand, within EX31_RS21125 at 868.598 kb on + strand, within EX31_RS21125 at 868.598 kb on + strand, within EX31_RS21125 at 868.598 kb on + strand, within EX31_RS21125 at 868.598 kb on + strand, within EX31_RS21125 at 868.599 kb on - strand, within EX31_RS21125 at 868.599 kb on - strand, within EX31_RS21125 at 868.719 kb on - strand, within EX31_RS21125 at 868.737 kb on - strand, within EX31_RS21125 at 868.802 kb on + strand, within EX31_RS21125 at 868.802 kb on + strand, within EX31_RS21125 at 868.802 kb on + strand, within EX31_RS21125 at 868.802 kb on + strand, within EX31_RS21125 at 868.803 kb on - strand, within EX31_RS21125 at 868.803 kb on - strand, within EX31_RS21125 at 868.803 kb on - strand, within EX31_RS21125 at 868.898 kb on - strand, within EX31_RS21125 at 868.899 kb on + strand, within EX31_RS21125 at 868.899 kb on + strand, within EX31_RS21125 at 869.060 kb on - strand, within EX31_RS21125 at 869.060 kb on - strand, within EX31_RS21125 at 869.083 kb on + strand, within EX31_RS21125 at 869.084 kb on - strand, within EX31_RS21125 at 869.085 kb on + strand, within EX31_RS21125 at 869.085 kb on + strand, within EX31_RS21125 at 869.085 kb on + strand, within EX31_RS21125 at 869.086 kb on - strand, within EX31_RS21125 at 869.086 kb on - strand, within EX31_RS21125 at 869.104 kb on + strand, within EX31_RS21125 at 869.104 kb on + strand, within EX31_RS21125 at 869.104 kb on + strand, within EX31_RS21125 at 869.105 kb on - strand, within EX31_RS21125 at 869.116 kb on + strand at 869.157 kb on + strand at 869.221 kb on + strand at 869.222 kb on - strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.236 kb on + strand at 869.237 kb on - strand at 869.237 kb on - strand at 869.237 kb on - strand at 869.237 kb on - strand at 869.237 kb on - strand at 869.237 kb on - strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.246 kb on + strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.247 kb on - strand at 869.271 kb on - strand at 869.276 kb on + strand at 869.317 kb on - strand at 869.317 kb on - strand at 869.317 kb on - strand at 869.361 kb on + strand at 869.361 kb on + strand at 869.362 kb on - strand at 869.375 kb on + strand at 869.376 kb on - strand at 869.395 kb on + strand at 869.395 kb on + strand at 869.396 kb on - strand at 869.401 kb on + strand at 869.401 kb on + strand at 869.401 kb on + strand at 869.402 kb on - strand at 869.815 kb on + strand, within EX31_RS21130 at 869.815 kb on + strand, within EX31_RS21130 at 870.000 kb on - strand, within EX31_RS21130 at 870.023 kb on - strand, within EX31_RS21130 at 870.080 kb on + strand, within EX31_RS21130 at 870.080 kb on + strand, within EX31_RS21130 at 870.080 kb on + strand, within EX31_RS21130 at 870.080 kb on + strand, within EX31_RS21130 at 870.081 kb on - strand, within EX31_RS21130 at 870.081 kb on - strand, within EX31_RS21130 at 870.081 kb on - strand, within EX31_RS21130 at 870.081 kb on - strand, within EX31_RS21130 at 870.121 kb on + strand, within EX31_RS21130 at 870.121 kb on + strand, within EX31_RS21130 at 870.121 kb on + strand, within EX31_RS21130 at 870.122 kb on - strand, within EX31_RS21130 at 870.122 kb on - strand, within EX31_RS21130 at 870.135 kb on - strand, within EX31_RS21130 at 870.283 kb on + strand, within EX31_RS21130 at 870.283 kb on + strand, within EX31_RS21130 at 870.339 kb on - strand, within EX31_RS21130 at 870.339 kb on - strand, within EX31_RS21130 at 870.397 kb on + strand, within EX31_RS21130 at 870.397 kb on + strand, within EX31_RS21130 at 870.398 kb on - strand, within EX31_RS21130 at 870.398 kb on - strand, within EX31_RS21130 at 870.460 kb on + strand, within EX31_RS21130 at 870.473 kb on + strand, within EX31_RS21130 at 870.473 kb on + strand, within EX31_RS21130 at 870.474 kb on - strand, within EX31_RS21130 at 870.564 kb on + strand, within EX31_RS21130 at 870.565 kb on - strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.624 kb on + strand, within EX31_RS21130 at 870.625 kb on - strand, within EX31_RS21130 at 870.625 kb on - strand, within EX31_RS21130 at 870.626 kb on + strand, within EX31_RS21130 at 870.626 kb on + strand, within EX31_RS21130 at 870.627 kb on - strand, within EX31_RS21130 at 870.627 kb on - strand, within EX31_RS21130 at 870.648 kb on - strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.708 kb on + strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.709 kb on - strand, within EX31_RS21130 at 870.766 kb on + strand, within EX31_RS21130 at 870.767 kb on - strand, within EX31_RS21130 at 870.953 kb on - strand at 870.989 kb on + strand at 870.989 kb on + strand at 871.021 kb on + strand at 871.022 kb on - strand at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.049 kb on + strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.050 kb on - strand, within EX31_RS21135 at 871.051 kb on + strand, within EX31_RS21135 at 871.051 kb on + strand, within EX31_RS21135 at 871.161 kb on + strand, within EX31_RS21135 at 871.161 kb on + strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.162 kb on - strand, within EX31_RS21135 at 871.218 kb on + strand at 871.218 kb on + strand at 871.218 kb on + strand at 871.218 kb on + strand at 871.219 kb on - strand at 871.219 kb on - strand at 871.238 kb on + strand at 871.238 kb on + strand at 871.238 kb on + strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.239 kb on - strand at 871.406 kb on + strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.407 kb on - strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.578 kb on + strand, within EX31_RS21140 at 871.588 kb on + strand, within EX31_RS21140 at 871.621 kb on + strand, within EX31_RS21140 at 871.622 kb on - strand, within EX31_RS21140 at 871.732 kb on - strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.889 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 868,395 - +1.2 868,395 - -0.2 868,405 + -1.8 868,405 + +0.3 868,405 + -0.7 868,406 - +0.5 868,406 - -0.7 868,406 - +0.9 868,406 - -2.2 868,429 + +0.7 868,429 + -0.0 868,430 - +0.6 868,430 - -0.7 868,482 + EX31_RS21125 0.15 -0.4 868,482 + EX31_RS21125 0.15 -0.2 868,482 + EX31_RS21125 0.15 +0.6 868,482 + EX31_RS21125 0.15 +0.8 868,482 + EX31_RS21125 0.15 +2.1 868,483 - EX31_RS21125 0.16 -2.8 868,483 - EX31_RS21125 0.16 +0.8 868,483 - EX31_RS21125 0.16 -0.0 868,543 + EX31_RS21125 0.23 -0.5 868,543 + EX31_RS21125 0.23 -1.4 868,544 - EX31_RS21125 0.23 +1.4 868,598 + EX31_RS21125 0.29 +0.4 868,598 + EX31_RS21125 0.29 +1.1 868,598 + EX31_RS21125 0.29 +0.1 868,598 + EX31_RS21125 0.29 +0.3 868,599 - EX31_RS21125 0.29 +0.3 868,599 - EX31_RS21125 0.29 +0.8 868,719 - EX31_RS21125 0.44 +0.0 868,737 - EX31_RS21125 0.46 +0.7 868,802 + EX31_RS21125 0.54 -1.1 868,802 + EX31_RS21125 0.54 +0.5 868,802 + EX31_RS21125 0.54 -0.7 868,802 + EX31_RS21125 0.54 -0.3 868,803 - EX31_RS21125 0.54 -1.6 868,803 - EX31_RS21125 0.54 +1.2 868,803 - EX31_RS21125 0.54 +0.7 868,898 - EX31_RS21125 0.65 +2.1 868,899 + EX31_RS21125 0.65 -0.6 868,899 + EX31_RS21125 0.65 -0.1 869,060 - EX31_RS21125 0.84 -0.2 869,060 - EX31_RS21125 0.84 -0.5 869,083 + EX31_RS21125 0.87 -1.1 869,084 - EX31_RS21125 0.87 -0.1 869,085 + EX31_RS21125 0.87 -0.8 869,085 + EX31_RS21125 0.87 +1.3 869,085 + EX31_RS21125 0.87 +0.5 869,086 - EX31_RS21125 0.87 -0.2 869,086 - EX31_RS21125 0.87 +1.2 869,104 + EX31_RS21125 0.90 -1.0 869,104 + EX31_RS21125 0.90 +0.1 869,104 + EX31_RS21125 0.90 -0.2 869,105 - EX31_RS21125 0.90 -0.2 869,116 + -0.3 869,157 + +0.7 869,221 + +0.6 869,222 - -0.2 869,236 + +0.3 869,236 + -0.8 869,236 + +0.5 869,236 + +1.3 869,236 + +1.0 869,236 + +0.3 869,236 + +0.3 869,236 + -0.2 869,236 + +0.0 869,236 + +0.6 869,236 + -2.5 869,236 + -0.3 869,236 + -0.2 869,237 - +0.5 869,237 - +0.4 869,237 - +1.2 869,237 - -1.0 869,237 - +0.2 869,237 - -0.8 869,246 + +1.1 869,246 + -1.4 869,246 + +0.7 869,246 + -0.1 869,246 + -0.7 869,246 + +1.0 869,246 + +0.4 869,247 - -0.7 869,247 - +0.2 869,247 - +0.4 869,247 - -1.4 869,247 - -0.4 869,247 - -0.4 869,247 - -1.0 869,247 - -0.1 869,271 - +2.1 869,276 + +0.5 869,317 - -0.7 869,317 - +0.4 869,317 - +1.0 869,361 + +1.4 869,361 + +0.2 869,362 - -0.7 869,375 + +0.5 869,376 - +0.2 869,395 + +0.2 869,395 + -0.2 869,396 - -1.0 869,401 + -0.2 869,401 + -0.5 869,401 + -0.4 869,402 - -0.6 869,815 + EX31_RS21130 0.27 -0.3 869,815 + EX31_RS21130 0.27 +0.2 870,000 - EX31_RS21130 0.38 +0.3 870,023 - EX31_RS21130 0.40 -0.2 870,080 + EX31_RS21130 0.43 +0.1 870,080 + EX31_RS21130 0.43 +0.5 870,080 + EX31_RS21130 0.43 +0.8 870,080 + EX31_RS21130 0.43 -0.1 870,081 - EX31_RS21130 0.44 +0.5 870,081 - EX31_RS21130 0.44 +0.5 870,081 - EX31_RS21130 0.44 -0.9 870,081 - EX31_RS21130 0.44 +0.1 870,121 + EX31_RS21130 0.46 +0.3 870,121 + EX31_RS21130 0.46 -0.4 870,121 + EX31_RS21130 0.46 -0.2 870,122 - EX31_RS21130 0.46 -0.3 870,122 - EX31_RS21130 0.46 -0.2 870,135 - EX31_RS21130 0.47 +0.1 870,283 + EX31_RS21130 0.56 +0.5 870,283 + EX31_RS21130 0.56 -1.3 870,339 - EX31_RS21130 0.60 +0.3 870,339 - EX31_RS21130 0.60 -0.1 870,397 + EX31_RS21130 0.63 -0.1 870,397 + EX31_RS21130 0.63 +1.2 870,398 - EX31_RS21130 0.64 -0.1 870,398 - EX31_RS21130 0.64 -0.8 870,460 + EX31_RS21130 0.67 -1.5 870,473 + EX31_RS21130 0.68 -0.6 870,473 + EX31_RS21130 0.68 -1.5 870,474 - EX31_RS21130 0.68 -0.6 870,564 + EX31_RS21130 0.74 +0.3 870,565 - EX31_RS21130 0.74 -0.5 870,624 + EX31_RS21130 0.78 -0.4 870,624 + EX31_RS21130 0.78 -2.7 870,624 + EX31_RS21130 0.78 -0.1 870,625 - EX31_RS21130 0.78 -1.4 870,625 - EX31_RS21130 0.78 -0.2 870,626 + EX31_RS21130 0.78 +0.0 870,626 + EX31_RS21130 0.78 +0.5 870,627 - EX31_RS21130 0.78 +1.2 870,627 - EX31_RS21130 0.78 +0.5 870,648 - EX31_RS21130 0.79 -0.4 870,708 + EX31_RS21130 0.83 -0.4 870,708 + EX31_RS21130 0.83 -0.2 870,708 + EX31_RS21130 0.83 +0.4 870,708 + EX31_RS21130 0.83 -0.3 870,708 + EX31_RS21130 0.83 -0.1 870,708 + EX31_RS21130 0.83 -1.2 870,708 + EX31_RS21130 0.83 +0.0 870,708 + EX31_RS21130 0.83 +0.5 870,708 + EX31_RS21130 0.83 +0.1 870,708 + EX31_RS21130 0.83 -1.2 870,708 + EX31_RS21130 0.83 -0.3 870,708 + EX31_RS21130 0.83 +0.3 870,708 + EX31_RS21130 0.83 +0.4 870,708 + EX31_RS21130 0.83 +0.7 870,708 + EX31_RS21130 0.83 -0.1 870,708 + EX31_RS21130 0.83 +0.6 870,709 - EX31_RS21130 0.83 -0.3 870,709 - EX31_RS21130 0.83 +0.1 870,709 - EX31_RS21130 0.83 -0.4 870,709 - EX31_RS21130 0.83 -0.6 870,709 - EX31_RS21130 0.83 -0.2 870,709 - EX31_RS21130 0.83 +2.1 870,709 - EX31_RS21130 0.83 -1.0 870,709 - EX31_RS21130 0.83 +1.0 870,709 - EX31_RS21130 0.83 -0.0 870,709 - EX31_RS21130 0.83 -0.6 870,709 - EX31_RS21130 0.83 +2.1 870,709 - EX31_RS21130 0.83 +0.0 870,709 - EX31_RS21130 0.83 -0.4 870,709 - EX31_RS21130 0.83 -0.0 870,766 + EX31_RS21130 0.87 +0.1 870,767 - EX31_RS21130 0.87 +1.1 870,953 - -0.6 870,989 + +0.1 870,989 + -0.2 871,021 + +0.2 871,022 - +1.6 871,049 + EX31_RS21135 0.18 +0.3 871,049 + EX31_RS21135 0.18 +0.4 871,049 + EX31_RS21135 0.18 +0.4 871,049 + EX31_RS21135 0.18 -1.0 871,050 - EX31_RS21135 0.19 +0.2 871,050 - EX31_RS21135 0.19 +1.0 871,050 - EX31_RS21135 0.19 -1.0 871,050 - EX31_RS21135 0.19 -1.1 871,050 - EX31_RS21135 0.19 -0.2 871,051 + EX31_RS21135 0.19 +1.0 871,051 + EX31_RS21135 0.19 -0.4 871,161 + EX31_RS21135 0.76 +0.4 871,161 + EX31_RS21135 0.76 -0.1 871,162 - EX31_RS21135 0.76 +1.8 871,162 - EX31_RS21135 0.76 +1.8 871,162 - EX31_RS21135 0.76 +0.6 871,218 + -0.2 871,218 + +0.3 871,218 + +2.1 871,218 + +1.1 871,219 - +0.1 871,219 - +0.3 871,238 + +0.5 871,238 + -0.0 871,238 + +0.0 871,239 - +0.2 871,239 - +0.3 871,239 - +0.4 871,239 - +1.4 871,239 - +0.6 871,239 - +0.8 871,406 + EX31_RS21140 0.14 +1.4 871,407 - EX31_RS21140 0.14 +1.2 871,407 - EX31_RS21140 0.14 -0.8 871,407 - EX31_RS21140 0.14 -0.7 871,407 - EX31_RS21140 0.14 +0.7 871,578 + EX31_RS21140 0.27 -1.0 871,578 + EX31_RS21140 0.27 +0.1 871,578 + EX31_RS21140 0.27 -0.2 871,578 + EX31_RS21140 0.27 -0.1 871,588 + EX31_RS21140 0.27 -0.8 871,621 + EX31_RS21140 0.30 +0.2 871,622 - EX31_RS21140 0.30 -0.1 871,732 - EX31_RS21140 0.38 +0.9 871,763 + EX31_RS21140 0.40 +0.0 871,763 + EX31_RS21140 0.40 -3.1 871,763 + EX31_RS21140 0.40 -0.2 871,763 + EX31_RS21140 0.40 -0.3 871,764 - EX31_RS21140 0.40 -0.9 871,764 - EX31_RS21140 0.40 +0.5 871,764 - EX31_RS21140 0.40 -0.3 871,764 - EX31_RS21140 0.40 +1.3 871,888 + EX31_RS21140 0.49 +1.1 871,888 + EX31_RS21140 0.49 -0.9 871,888 + EX31_RS21140 0.49 -0.1 871,889 - EX31_RS21140 0.49 +0.1 871,899 - EX31_RS21140 0.50 +1.6 871,899 - EX31_RS21140 0.50 +1.6 871,899 - EX31_RS21140 0.50 -0.3 871,965 + EX31_RS21140 0.54 +0.6 871,965 + EX31_RS21140 0.54 -0.5
Or see this region's nucleotide sequence